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The map label for this gene is ada [H]

Identifier: 13471777

GI number: 13471777

Start: 1534955

End: 1535509

Strand: Direct

Name: ada [H]

Synonym: mlr1860

Alternate gene names: 13471777

Gene position: 1534955-1535509 (Clockwise)

Preceding gene: 13471776

Following gene: 13471778

Centisome position: 21.82

GC content: 69.73

Gene sequence:

>555_bases
ATGAACCCGATACAAACCCACATGCCGACGACAGCCCGCAACACCGCCACCCTCGACACCGTCGCCTACGCCGTCGGCCA
ATCGGCGATCGGCAAGATCCTGGCGGCGCGCAGCGCGATCGGGGTCTGCGCCATCCTGATCGGCTCCGACGCCGAGGCGC
TGGAGCAGGACCTCGCCGACCGTTTCCCCGGCAAGGTGCTGGTCGAAGACGAGGCCGCCCTGCGCGGCGATCTCGCGGCG
ATTGCCCGCTTCATCGAAACGCCCGGCGCCGGCCTTGGCCTGCCGCTCGACATGCGCAACGGCACGCCGTTCCAGCAAAG
AGTGTGGGAGGTGTTGCGCGCCATCCCCTGTGGCGCCACCATCACCTACACCGCGCTTGCCCGGCGCCTTGGCCGGCCGA
ACGGCGCGCGTGCCGTGGCGACCGCCTGTGCCGCCAACGCCATCGCGCTCGGCATTCCCTGCCACCGCGTTGTCCGTGCC
GACGGCACGCTGTCGGGCTACCGCTGGGGCATCGAACGCAAGCGCGCTTTGCTCGATAAGGAGGCCGCGATATGA

Upstream 100 bases:

>100_bases
AGCCCCTCAAACCCTGAGATTTGAATTTGAAAGCAAGCATCGGAGTGAGGGACGCAAGGCACGCGGCTAGGGTTCAAGCA
CAAACACAGGAGCGAAGACA

Downstream 100 bases:

>100_bases
ACGCCCACGCCAAGGTTGCCGCACCCGTTGTGGAAGCCGCCGAAACACGCGTCGCCACTTACGACTGGCCGGCGCTGGCC
GCCGAACTCGACGGCTTCGG

Product: O6-methylguanine-DNA methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MNPIQTHMPTTARNTATLDTVAYAVGQSAIGKILAARSAIGVCAILIGSDAEALEQDLADRFPGKVLVEDEAALRGDLAA
IARFIETPGAGLGLPLDMRNGTPFQQRVWEVLRAIPCGATITYTALARRLGRPNGARAVATACAANAIALGIPCHRVVRA
DGTLSGYRWGIERKRALLDKEAAI

Sequences:

>Translated_184_residues
MNPIQTHMPTTARNTATLDTVAYAVGQSAIGKILAARSAIGVCAILIGSDAEALEQDLADRFPGKVLVEDEAALRGDLAA
IARFIETPGAGLGLPLDMRNGTPFQQRVWEVLRAIPCGATITYTALARRLGRPNGARAVATACAANAIALGIPCHRVVRA
DGTLSGYRWGIERKRALLDKEAAI
>Mature_184_residues
MNPIQTHMPTTARNTATLDTVAYAVGQSAIGKILAARSAIGVCAILIGSDAEALEQDLADRFPGKVLVEDEAALRGDLAA
IARFIETPGAGLGLPLDMRNGTPFQQRVWEVLRAIPCGATITYTALARRLGRPNGARAVATACAANAIALGIPCHRVVRA
DGTLSGYRWGIERKRALLDKEAAI

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=162, Percent_Identity=47.5308641975309, Blast_Score=153, Evalue=7e-39,
Organism=Escherichia coli, GI1787596, Length=84, Percent_Identity=50, Blast_Score=95, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=48.2352941176471, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=50, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=88, Percent_Identity=40.9090909090909, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137554, Length=145, Percent_Identity=38.6206896551724, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19386; Mature: 19386

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00374 MGMT ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIQTHMPTTARNTATLDTVAYAVGQSAIGKILAARSAIGVCAILIGSDAEALEQDLAD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHH
RFPGKVLVEDEAALRGDLAAIARFIETPGAGLGLPLDMRNGTPFQQRVWEVLRAIPCGAT
HCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCH
ITYTALARRLGRPNGARAVATACAANAIALGIPCHRVVRADGTLSGYRWGIERKRALLDK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
EAAI
HCCC
>Mature Secondary Structure
MNPIQTHMPTTARNTATLDTVAYAVGQSAIGKILAARSAIGVCAILIGSDAEALEQDLAD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHH
RFPGKVLVEDEAALRGDLAAIARFIETPGAGLGLPLDMRNGTPFQQRVWEVLRAIPCGAT
HCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCH
ITYTALARRLGRPNGARAVATACAANAIALGIPCHRVVRADGTLSGYRWGIERKRALLDK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
EAAI
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]