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The map label for this gene is potB [H]

Identifier: 13471691

GI number: 13471691

Start: 1454832

End: 1455701

Strand: Reverse

Name: potB [H]

Synonym: mll1740

Alternate gene names: 13471691

Gene position: 1455701-1454832 (Counterclockwise)

Preceding gene: 13471692

Following gene: 13471690

Centisome position: 20.69

GC content: 63.33

Gene sequence:

>870_bases
ATGGCAGCACAAAGCGCGACCGCATCGCGCAGCGGGTTGAGATCGGCTCTGCCGCTGCTGGCGCCGGCCTATCTCTGGCT
GACGGTGGCGATCTTCCTGCCGCTGTCGGCCATGGTCTTTTTCTCCTTCATGACCGACCTGCCGTTGTCGGGAAAGCCCT
GGGCTTTCACCCTTGGCAACTACGCCGCCTTCTTCTCGCAGAGCCTTTATCTCACGCTGCTGCTCGCCTCGCTGCGGCTT
GGGCTCGAAGTGACGCTGTGGTGCGTCGTCATCGGCTATCCCGCCGCTTACGTGCTAGCCAAGGTGCTGAAGGGCCGCAG
CCGCGAAGCGATCTTCCTGCTGGTCATCCTGCCGTTCTGGTCGAACGGGCTGGTGCGCATCTTCTCCTGGGCGATGGTTT
TGCGCGAAGGCGGCATACTCGATACGGCGCTGAACGCCGTGCTGCCGTTCAAGATCAACATCGATCTGATGTATTCCTAT
CCGGCCGTCATCATCGGGCTGGTGCACTCCTACGTGCCCTACATGGTGCTGACCTGTTATCTCACCTTGCAGGCGATCGA
CGATTCGCTGATCGAGGCCGGGCGTTCGCTTGGCGCCTCGCGGCTGCAGGTGCTGAAACGGGTGATCATCCCGCTGTCCA
TGCCGGGACTGGTGGCAGGCGCGGCACTGATCTTCGTGCCGGTTGTCGGCTCGTTCATGGAACCGCGCATCCTCGGCGGC
CGCACCGGCACCTTCTACGGCACTGTGATCGAGGACCAGTTCGTCGCCGTCTTCAACTGGCCGCTGGGAGCGGCGCTCTC
CTTCATCCTGCTCGCCGTCGTGCTGGTCATCCTGGCGATTGCCTCGCCGGTGTTGCGGAGGGCCGCGTGA

Upstream 100 bases:

>100_bases
CTCTGGCAGCAGCTCAAGGTCGACGTGAAATAAAACCCACGTCCGGACCAGCCGGCGGTTCGCGCCGGCTGGTGCTTTCA
ATCCTGGGGAGGAGGGTGGC

Downstream 100 bases:

>100_bases
TGGCGACCACCCGCATCCTCGAAGGGCTCGGCCGCCTCTATATCTTGCTGCTGCTCGCCTTTCTCTATCTGCCCATCATC
ATCATGGCGCTGATGTCGTT

Product: permease protein of polyamine ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAAQSATASRSGLRSALPLLAPAYLWLTVAIFLPLSAMVFFSFMTDLPLSGKPWAFTLGNYAAFFSQSLYLTLLLASLRL
GLEVTLWCVVIGYPAAYVLAKVLKGRSREAIFLLVILPFWSNGLVRIFSWAMVLREGGILDTALNAVLPFKINIDLMYSY
PAVIIGLVHSYVPYMVLTCYLTLQAIDDSLIEAGRSLGASRLQVLKRVIIPLSMPGLVAGAALIFVPVVGSFMEPRILGG
RTGTFYGTVIEDQFVAVFNWPLGAALSFILLAVVLVILAIASPVLRRAA

Sequences:

>Translated_289_residues
MAAQSATASRSGLRSALPLLAPAYLWLTVAIFLPLSAMVFFSFMTDLPLSGKPWAFTLGNYAAFFSQSLYLTLLLASLRL
GLEVTLWCVVIGYPAAYVLAKVLKGRSREAIFLLVILPFWSNGLVRIFSWAMVLREGGILDTALNAVLPFKINIDLMYSY
PAVIIGLVHSYVPYMVLTCYLTLQAIDDSLIEAGRSLGASRLQVLKRVIIPLSMPGLVAGAALIFVPVVGSFMEPRILGG
RTGTFYGTVIEDQFVAVFNWPLGAALSFILLAVVLVILAIASPVLRRAA
>Mature_288_residues
AAQSATASRSGLRSALPLLAPAYLWLTVAIFLPLSAMVFFSFMTDLPLSGKPWAFTLGNYAAFFSQSLYLTLLLASLRLG
LEVTLWCVVIGYPAAYVLAKVLKGRSREAIFLLVILPFWSNGLVRIFSWAMVLREGGILDTALNAVLPFKINIDLMYSYP
AVIIGLVHSYVPYMVLTCYLTLQAIDDSLIEAGRSLGASRLQVLKRVIIPLSMPGLVAGAALIFVPVVGSFMEPRILGGR
TGTFYGTVIEDQFVAVFNWPLGAALSFILLAVVLVILAIASPVLRRAA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=249, Percent_Identity=40.1606425702811, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787080, Length=220, Percent_Identity=36.8181818181818, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787713, Length=270, Percent_Identity=27.4074074074074, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31338; Mature: 31207

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQSATASRSGLRSALPLLAPAYLWLTVAIFLPLSAMVFFSFMTDLPLSGKPWAFTLGN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHH
YAAFFSQSLYLTLLLASLRLGLEVTLWCVVIGYPAAYVLAKVLKGRSREAIFLLVILPFW
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEECCC
SNGLVRIFSWAMVLREGGILDTALNAVLPFKINIDLMYSYPAVIIGLVHSYVPYMVLTCY
CCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
LTLQAIDDSLIEAGRSLGASRLQVLKRVIIPLSMPGLVAGAALIFVPVVGSFMEPRILGG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEECC
RTGTFYGTVIEDQFVAVFNWPLGAALSFILLAVVLVILAIASPVLRRAA
CCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AAQSATASRSGLRSALPLLAPAYLWLTVAIFLPLSAMVFFSFMTDLPLSGKPWAFTLGN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHH
YAAFFSQSLYLTLLLASLRLGLEVTLWCVVIGYPAAYVLAKVLKGRSREAIFLLVILPFW
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEECCC
SNGLVRIFSWAMVLREGGILDTALNAVLPFKINIDLMYSYPAVIIGLVHSYVPYMVLTCY
CCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
LTLQAIDDSLIEAGRSLGASRLQVLKRVIIPLSMPGLVAGAALIFVPVVGSFMEPRILGG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEECC
RTGTFYGTVIEDQFVAVFNWPLGAALSFILLAVVLVILAIASPVLRRAA
CCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]