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The map label for this gene is potD [H]

Identifier: 13471692

GI number: 13471692

Start: 1455769

End: 1456830

Strand: Reverse

Name: potD [H]

Synonym: mll1741

Alternate gene names: 13471692

Gene position: 1456830-1455769 (Counterclockwise)

Preceding gene: 13471693

Following gene: 13471691

Centisome position: 20.71

GC content: 60.83

Gene sequence:

>1062_bases
ATGCGCATGCCAAGACTGACTGCTCTCTTGACGGCGACCGCCGTCTTCACCACCGCACTCACGCTGGCCGCCAGTGCCGC
CGAAGTGCATGTGCTCAACTGGAAAGGCTACGGCGCCGACGAGCCCTGGGCGGTCGCCGCCTTCGAGAAGGCGACCGGCA
ACAAGGTCGTCAACGACTTCTTCAATTCCGAACAGGAAATGCTGACCAAGATCCGGACCAATCCGGGGCTTTACGACGTG
GTCATGATCAACGCCGCCTTCAATGACCAGGCGATGGCGGAAAAACTGATCCAGCCGATCGATACCTCGAAGCTGCCGAA
CTATGCCGACATCAGCAAGGACAAAGCCGGTTCGCCGATGCTCGACCATGACGGCAAGGTCTATGGCGTGCCATGGGTGT
GGGGCCTGACGGCGCTCGCCATCAATGAAAAATCCTTCGACAAGCCGCCGACGTCGATCGCCGAAATGTGGGATCCCAAG
CACAAGGGCCGCGTCGTCATTCGCGACGACGCCGTCGAGGCGGTGCAGTTCGGCGCTATCGCCACCGGCCAGAACATCAA
CGACATCAAGGATATGGATGCGGTCAAGACGAAGCTCACCTCGCTGATGCCGCAGATCAAGACCTTCTGGAGCTCGGAGA
ACGACTGGAACCAGATGGTCGCCTCCAACCAGATCGACATCGGCACCTATTGGAGCGGCTCGGCCGACCGTGCCAAGACG
CATTTCAAGCTGCCGGTTTCGCTGGTCATTCCGCAGGAAGGCGCCGTCGCCTGGCTGGATGCCTTCTCCATTCCGGTCGG
CTCCAAGAATGTCGAAGGCGCGCAGGCCTTCATCAACTGGATGATCGACCCGAAATTCTACGTCGAATGGGTCACCAAGG
TCGGCGCGCCTGTCTCGGCAAACACCAAGGCGGTGGAGGCGCTGCCGCAGGATGCCTTCAACCGCAAGGTGATGGGCGAT
CCCGAGGTCGCCAAGCGCATCCAGTTCCAGGCGCCGATCACCGATGCCCAGCGCGAGGCCTACCTGGCGCTCTGGCAGCA
GCTCAAGGTCGACGTGAAATAA

Upstream 100 bases:

>100_bases
GAGCCAGGCCGCCGCGACCGGCACGCCGGGCCAGAACCAGAAGGCCCATCAGACAGCCTGACAAGCCCCGCCAAGCGGGC
AAACAAAGAGAGGAACCGAC

Downstream 100 bases:

>100_bases
AACCCACGTCCGGACCAGCCGGCGGTTCGCGCCGGCTGGTGCTTTCAATCCTGGGGAGGAGGGTGGCATGGCAGCACAAA
GCGCGACCGCATCGCGCAGC

Product: periplasmic spermidine/putrescine-binding protein or ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MRMPRLTALLTATAVFTTALTLAASAAEVHVLNWKGYGADEPWAVAAFEKATGNKVVNDFFNSEQEMLTKIRTNPGLYDV
VMINAAFNDQAMAEKLIQPIDTSKLPNYADISKDKAGSPMLDHDGKVYGVPWVWGLTALAINEKSFDKPPTSIAEMWDPK
HKGRVVIRDDAVEAVQFGAIATGQNINDIKDMDAVKTKLTSLMPQIKTFWSSENDWNQMVASNQIDIGTYWSGSADRAKT
HFKLPVSLVIPQEGAVAWLDAFSIPVGSKNVEGAQAFINWMIDPKFYVEWVTKVGAPVSANTKAVEALPQDAFNRKVMGD
PEVAKRIQFQAPITDAQREAYLALWQQLKVDVK

Sequences:

>Translated_353_residues
MRMPRLTALLTATAVFTTALTLAASAAEVHVLNWKGYGADEPWAVAAFEKATGNKVVNDFFNSEQEMLTKIRTNPGLYDV
VMINAAFNDQAMAEKLIQPIDTSKLPNYADISKDKAGSPMLDHDGKVYGVPWVWGLTALAINEKSFDKPPTSIAEMWDPK
HKGRVVIRDDAVEAVQFGAIATGQNINDIKDMDAVKTKLTSLMPQIKTFWSSENDWNQMVASNQIDIGTYWSGSADRAKT
HFKLPVSLVIPQEGAVAWLDAFSIPVGSKNVEGAQAFINWMIDPKFYVEWVTKVGAPVSANTKAVEALPQDAFNRKVMGD
PEVAKRIQFQAPITDAQREAYLALWQQLKVDVK
>Mature_353_residues
MRMPRLTALLTATAVFTTALTLAASAAEVHVLNWKGYGADEPWAVAAFEKATGNKVVNDFFNSEQEMLTKIRTNPGLYDV
VMINAAFNDQAMAEKLIQPIDTSKLPNYADISKDKAGSPMLDHDGKVYGVPWVWGLTALAINEKSFDKPPTSIAEMWDPK
HKGRVVIRDDAVEAVQFGAIATGQNINDIKDMDAVKTKLTSLMPQIKTFWSSENDWNQMVASNQIDIGTYWSGSADRAKT
HFKLPVSLVIPQEGAVAWLDAFSIPVGSKNVEGAQAFINWMIDPKFYVEWVTKVGAPVSANTKAVEALPQDAFNRKVMGD
PEVAKRIQFQAPITDAQREAYLALWQQLKVDVK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=326, Percent_Identity=31.5950920245399, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787078, Length=304, Percent_Identity=29.9342105263158, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787711, Length=257, Percent_Identity=26.4591439688716, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38992; Mature: 38992

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMPRLTALLTATAVFTTALTLAASAAEVHVLNWKGYGADEPWAVAAFEKATGNKVVNDF
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEHHCCCCHHHHHH
FNSEQEMLTKIRTNPGLYDVVMINAAFNDQAMAEKLIQPIDTSKLPNYADISKDKAGSPM
HCCHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
LDHDGKVYGVPWVWGLTALAINEKSFDKPPTSIAEMWDPKHKGRVVIRDDAVEAVQFGAI
CCCCCCEEECCHHHHHEEEEECCCCCCCCCHHHHHHCCCCCCCEEEEECCCHHHHHHCCE
ATGQNINDIKDMDAVKTKLTSLMPQIKTFWSSENDWNQMVASNQIDIGTYWSGSADRAKT
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEECEEECCCCCCCCE
HFKLPVSLVIPQEGAVAWLDAFSIPVGSKNVEGAQAFINWMIDPKFYVEWVTKVGAPVSA
EEECCEEEEECCCCCEEEEHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCC
NTKAVEALPQDAFNRKVMGDPEVAKRIQFQAPITDAQREAYLALWQQLKVDVK
CCHHHHHCCHHHHCCCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRMPRLTALLTATAVFTTALTLAASAAEVHVLNWKGYGADEPWAVAAFEKATGNKVVNDF
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEHHCCCCHHHHHH
FNSEQEMLTKIRTNPGLYDVVMINAAFNDQAMAEKLIQPIDTSKLPNYADISKDKAGSPM
HCCHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
LDHDGKVYGVPWVWGLTALAINEKSFDKPPTSIAEMWDPKHKGRVVIRDDAVEAVQFGAI
CCCCCCEEECCHHHHHEEEEECCCCCCCCCHHHHHHCCCCCCCEEEEECCCHHHHHHCCE
ATGQNINDIKDMDAVKTKLTSLMPQIKTFWSSENDWNQMVASNQIDIGTYWSGSADRAKT
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEECEEECCCCCCCCE
HFKLPVSLVIPQEGAVAWLDAFSIPVGSKNVEGAQAFINWMIDPKFYVEWVTKVGAPVSA
EEECCEEEEECCCCCEEEEHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCC
NTKAVEALPQDAFNRKVMGDPEVAKRIQFQAPITDAQREAYLALWQQLKVDVK
CCHHHHHCCHHHHCCCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]