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The map label for this gene is ygfT [H]

Identifier: 13471619

GI number: 13471619

Start: 1364695

End: 1366050

Strand: Reverse

Name: ygfT [H]

Synonym: mll1646

Alternate gene names: 13471619

Gene position: 1366050-1364695 (Counterclockwise)

Preceding gene: 13471620

Following gene: 13471618

Centisome position: 19.41

GC content: 65.71

Gene sequence:

>1356_bases
ATGCCAGAAGGCCAGTTCAAGGAAGGTATCGTCGGCGGCAGGCTTGCGGCCGAGCAGTACGCCGATAATTTTTCCGACCT
GCATCCGCCGCTCGATCATCATGAGGGGTTGGTCGAATCCGACCGCTGCTATTTCTGCTATGACGCGCCATGCATGAACG
CGTGCCCGACCTCGATCGACATCCCGCTGTTTATCCGTCAGATCTCGACCGGCAACCCGATCGGCTCAGCCAAGACCATC
TTCGACCAGAACATTTTGGGCGGCATGTGCGCTCGCGTCTGCCCGACCGAGACGCTGTGCGAAGAGGTCTGCGTGCGCGA
GGTGGCGGAAGGCAAGCCGGTGCAGATCGGACGCCTGCAGCGCTACGCCACCGATGTCGCCATGGCCGAGAACAAGCAGT
TCTACCCGCGCGCGGAGCCGACCGGAAAGACGGTCGCCGTGGTCGGGGCCGGCCCGGCCGGCCTTGCCGCCGCGCACCGG
CTCGCCCGTCATGGCCATGACGTAACCATACTGGAAGCACGGCCGAAGGCCGGCGGCCTCAACGAATACGGCATCGCCGC
CTATAAGAGCGTCGACAATTTCGCCCAGGCCGAGGTCGAATATGTCACCGCGATCGGCGGCATCGACATCCAGAACGGCA
AGGCGCTCGGCCGCGACTACCAGCTTTCCGACCTGATCCGCAACTACGACGCGGTGTTCCTCGGCATGGGGCTTGGCGGC
GTCAACGCGCTGCGCGCCGATGGCGAGGATGCCGAAGGCGTCACCAATGCGGTGGAGTTCATCGCCGAGCTGCGCCAGGC
CAGCGATCTCTCCGGCCTGCCGGTCGGCCGGCGCGTCGTCGTCATCGGCGGCGGCATGACGGCGATCGACGCCGCCGTGC
AGTCTAAGCTGCTCGGCGCCGAGGAGGTGACGATCTGCTACCGTCGTGGTCAGGAGCACATGAACGCTTCTGGGTTCGAG
CAGGATCTTGCCGCCGCCAACGGCGTCACCATCCGCCACTGGCTGCAGCCGAAGCGGGTGATCGCCGAGGCCGGCCAGGT
GTCGGGCATCGAACTCGAATACACGGCATTGAAGGGCGACAAGCTCGCCGGCACCGGCGAGACGTTGACGCTCGTCGCCG
ACCAGGTGTTCAAGGCGATCGGCCAGAGCTTTGTCCCGGCCGCGCTCAACGGCAGCGAGGCCTCGATCGAGCTCGAAGCC
GGCCGCATCAAGGTCGACGGGGAAGGGCGGACATCGCTGGCGAAAGTCTGGGCCGGCGGCGACTGCATCTTTGGCGGTGA
CGACCTGACCGTCTCGGCCGTGGCGCAAGGGCGCGATGCGGCGGAAAGCATACACAGGAGTCTGACGCAGGGATAA

Upstream 100 bases:

>100_bases
TTTATCAAGGCTTTTCAGCAGCTTGCGCCGAAAATTGACCAATTGATAAAAAAATAAAACGTGCTAGCCTTCCCCAAAAC
GAAAACATGGGGAACTGACA

Downstream 100 bases:

>100_bases
GGATCATGGCCACGGTGGAACCGGGCATCGCCCGTCACTACGACATAGCGGGTCTCGAGCAGCGCATCCTTGCCGCGCTC
GCCGATACGGGCGTGGACGT

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MPEGQFKEGIVGGRLAAEQYADNFSDLHPPLDHHEGLVESDRCYFCYDAPCMNACPTSIDIPLFIRQISTGNPIGSAKTI
FDQNILGGMCARVCPTETLCEEVCVREVAEGKPVQIGRLQRYATDVAMAENKQFYPRAEPTGKTVAVVGAGPAGLAAAHR
LARHGHDVTILEARPKAGGLNEYGIAAYKSVDNFAQAEVEYVTAIGGIDIQNGKALGRDYQLSDLIRNYDAVFLGMGLGG
VNALRADGEDAEGVTNAVEFIAELRQASDLSGLPVGRRVVVIGGGMTAIDAAVQSKLLGAEEVTICYRRGQEHMNASGFE
QDLAAANGVTIRHWLQPKRVIAEAGQVSGIELEYTALKGDKLAGTGETLTLVADQVFKAIGQSFVPAALNGSEASIELEA
GRIKVDGEGRTSLAKVWAGGDCIFGGDDLTVSAVAQGRDAAESIHRSLTQG

Sequences:

>Translated_451_residues
MPEGQFKEGIVGGRLAAEQYADNFSDLHPPLDHHEGLVESDRCYFCYDAPCMNACPTSIDIPLFIRQISTGNPIGSAKTI
FDQNILGGMCARVCPTETLCEEVCVREVAEGKPVQIGRLQRYATDVAMAENKQFYPRAEPTGKTVAVVGAGPAGLAAAHR
LARHGHDVTILEARPKAGGLNEYGIAAYKSVDNFAQAEVEYVTAIGGIDIQNGKALGRDYQLSDLIRNYDAVFLGMGLGG
VNALRADGEDAEGVTNAVEFIAELRQASDLSGLPVGRRVVVIGGGMTAIDAAVQSKLLGAEEVTICYRRGQEHMNASGFE
QDLAAANGVTIRHWLQPKRVIAEAGQVSGIELEYTALKGDKLAGTGETLTLVADQVFKAIGQSFVPAALNGSEASIELEA
GRIKVDGEGRTSLAKVWAGGDCIFGGDDLTVSAVAQGRDAAESIHRSLTQG
>Mature_450_residues
PEGQFKEGIVGGRLAAEQYADNFSDLHPPLDHHEGLVESDRCYFCYDAPCMNACPTSIDIPLFIRQISTGNPIGSAKTIF
DQNILGGMCARVCPTETLCEEVCVREVAEGKPVQIGRLQRYATDVAMAENKQFYPRAEPTGKTVAVVGAGPAGLAAAHRL
ARHGHDVTILEARPKAGGLNEYGIAAYKSVDNFAQAEVEYVTAIGGIDIQNGKALGRDYQLSDLIRNYDAVFLGMGLGGV
NALRADGEDAEGVTNAVEFIAELRQASDLSGLPVGRRVVVIGGGMTAIDAAVQSKLLGAEEVTICYRRGQEHMNASGFEQ
DLAAANGVTIRHWLQPKRVIAEAGQVSGIELEYTALKGDKLAGTGETLTLVADQVFKAIGQSFVPAALNGSEASIELEAG
RIKVDGEGRTSLAKVWAGGDCIFGGDDLTVSAVAQGRDAAESIHRSLTQG

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=457, Percent_Identity=33.0415754923414, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI237757300, Length=111, Percent_Identity=43.2432432432432, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI87082180, Length=459, Percent_Identity=35.9477124183007, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789606, Length=450, Percent_Identity=35.3333333333333, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788468, Length=426, Percent_Identity=34.7417840375587, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1788811, Length=457, Percent_Identity=33.4792122538293, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1789243, Length=438, Percent_Identity=29.6803652968037, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71984108, Length=462, Percent_Identity=30.7359307359307, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17570289, Length=454, Percent_Identity=26.6519823788546, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320030, Length=465, Percent_Identity=25.1612903225806, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640763, Length=450, Percent_Identity=33.3333333333333, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI18858217, Length=450, Percent_Identity=33.3333333333333, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24665547, Length=449, Percent_Identity=29.6213808463252, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24665543, Length=449, Percent_Identity=29.6213808463252, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28574881, Length=449, Percent_Identity=29.6213808463252, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24665539, Length=449, Percent_Identity=29.6213808463252, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00037 Fer4; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 47707; Mature: 47576

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGQFKEGIVGGRLAAEQYADNFSDLHPPLDHHEGLVESDRCYFCYDAPCMNACPTSID
CCCCCCCCCCCCCHHHHHHHHCCHHHCCCCHHHHCCCCCCCCEEEEECCCCCCCCCCCCC
IPLFIRQISTGNPIGSAKTIFDQNILGGMCARVCPTETLCEEVCVREVAEGKPVQIGRLQ
CHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHH
RYATDVAMAENKQFYPRAEPTGKTVAVVGAGPAGLAAAHRLARHGHDVTILEARPKAGGL
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCC
NEYGIAAYKSVDNFAQAEVEYVTAIGGIDIQNGKALGRDYQLSDLIRNYDAVFLGMGLGG
CCCCHHHHHHHHHHHHHHHEEEEEECCEEECCCCCCCCCCHHHHHHHCCCEEEEECCCCC
VNALRADGEDAEGVTNAVEFIAELRQASDLSGLPVGRRVVVIGGGMTAIDAAVQSKLLGA
CHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCC
EEVTICYRRGQEHMNASGFEQDLAAANGVTIRHWLQPKRVIAEAGQVSGIELEYTALKGD
HHHHHHHHCCHHHCCCCCCHHHHHHCCCCEEEECCCHHHHHHHCCCCCCEEEEEEEECCC
KLAGTGETLTLVADQVFKAIGQSFVPAALNGSEASIELEAGRIKVDGEGRTSLAKVWAGG
CCCCCCCCHHHHHHHHHHHHCHHCCCCCCCCCCCEEEEECCEEEECCCCCCHHHHEECCC
DCIFGGDDLTVSAVAQGRDAAESIHRSLTQG
CEEECCCCCEEEEHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PEGQFKEGIVGGRLAAEQYADNFSDLHPPLDHHEGLVESDRCYFCYDAPCMNACPTSID
CCCCCCCCCCCCHHHHHHHHCCHHHCCCCHHHHCCCCCCCCEEEEECCCCCCCCCCCCC
IPLFIRQISTGNPIGSAKTIFDQNILGGMCARVCPTETLCEEVCVREVAEGKPVQIGRLQ
CHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHHHH
RYATDVAMAENKQFYPRAEPTGKTVAVVGAGPAGLAAAHRLARHGHDVTILEARPKAGGL
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCC
NEYGIAAYKSVDNFAQAEVEYVTAIGGIDIQNGKALGRDYQLSDLIRNYDAVFLGMGLGG
CCCCHHHHHHHHHHHHHHHEEEEEECCEEECCCCCCCCCCHHHHHHHCCCEEEEECCCCC
VNALRADGEDAEGVTNAVEFIAELRQASDLSGLPVGRRVVVIGGGMTAIDAAVQSKLLGA
CHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCC
EEVTICYRRGQEHMNASGFEQDLAAANGVTIRHWLQPKRVIAEAGQVSGIELEYTALKGD
HHHHHHHHCCHHHCCCCCCHHHHHHCCCCEEEECCCHHHHHHHCCCCCCEEEEEEEECCC
KLAGTGETLTLVADQVFKAIGQSFVPAALNGSEASIELEAGRIKVDGEGRTSLAKVWAGG
CCCCCCCCHHHHHHHHHHHHCHHCCCCCCCCCCCEEEEECCEEEECCCCCCHHHHEECCC
DCIFGGDDLTVSAVAQGRDAAESIHRSLTQG
CEEECCCCCEEEEHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]