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The map label for this gene is kefA [C]

Identifier: 13471329

GI number: 13471329

Start: 1068205

End: 1070757

Strand: Reverse

Name: kefA [C]

Synonym: mll1272

Alternate gene names: 13471329

Gene position: 1070757-1068205 (Counterclockwise)

Preceding gene: 13471335

Following gene: 13471326

Centisome position: 15.22

GC content: 62.79

Gene sequence:

>2553_bases
ATGGCCGTCTTTATCAAGGACAGGCTTTTAAATGCCCTTGCCGCCGGTGAGTCGCCGATAGTCATGCTTTTGAGATTGCT
TCGCCTCGTTTTGGTTTTCACGCTGGTCATTTCGGCGTCGCCTTCGTTCGAGGCGATGGCGCAGGCGCTTGGCCAGGGCT
CCGCCGGCTTGATCACCGATCAGCAGAAAGTCATCCAGGGCCTGACTGCCAAGACCGACGATCTCGAGAAGAAGATACAG
CAGAACGGCGAGGACGATGCCTCGCTTGTGGACATCCGTCTGCAACTGGAGGATCTGTCGCGGGCGGCGCTGACCAGCGC
GCTGGTGTTCCGTCCACGGCTTACCGACATCAACAATCGCATCGAGGTGCTTGGTCCGCCGCCGGCCGCGGGCCAGCCGC
AGGAGCCCGACATCGTGACCGGCGAGCGCCAGGCGCTGGCGTCGGAAAAGGCCGAGATCAACGCGGTCGTCGCCAGCGCG
CAGAACCTGTCGATCCGCATCAGCGGGCTGATCGACAAGATCGGCAATATGCGCAGCGAGCAGTTCCGCAACTTTTTGAC
CAAGCGCTACGCACTGTCCGAGGCGCTGAGCCCACAGGTGTTTTCCGACGCCCGGGACGAATACGCCAACTTTTACAAGG
CGGTGTCGTCTTGGCTGAGCTTCGCGTTCAAGTTCAAGTTCCAGGCCATCCTCGCGGCGACCTTCGTGGCGCTCGGGCTG
GCGCTGGTGCTGCTGGTCGGCGGCAGGCGCCTGTTCGGGCGCATATTCGAAGCCGATCCGGCAGTCGAGGATCCGTCCTA
TCTCAGCCGCCTGTCCGTAGCATTCTGGTCGACCTTGCTGCCGACGCTGGCGGTCGGCGTGTTCCTGGCCTCCACGGTGT
TCTTTTTCAACTATTACAATGTGTTGCGCGGCGACATCGGCGTCTTCCTGAATGCGCTGGCGGCAGTCATCGCGGTGGTG
TTCTGCGTCAACCGGTTGACCAATGCCGCGCTTGAGCCGCGGCTGCCGAACTGGCGGCTCATTCCCGTCCAAACCGGCCC
GGCGCGCTGGCTGGTGCGGCTGACAACCGCCATGGCCGTGGTCATCGGCGTCAACAATTTCCTGTCGATCGTCAACGACA
AGATGGGCTCGCCACTGTCGCTGACCATCGCCCGCAGTTTCGTCGCCACCATTGTCGTCGGCATCATCCTGATCCTGATG
GCGATGCTGCGGCCGTTCAAGGCACGCGACGGAAGCTGGCGGCCATGGCCGGCTTGGCTGCGCTATCTGGCGCTGGCGCT
TGGCCTGTTCACCATCGCCGCCGCCCTGCTTGGCTATATCGGCCTTGCCCTGTTCGTCTCGCTGCAGGTGGTGGTCACAG
GCACGGCGCTGATCACCGCCTATATCGGCTTCCTGTCGGCGCAGGCGATCGGCGAGGAGGGGGCGTTCGCCAACACCACT
GTCGGGCGCTGGCTGTCGGCCAACTCCAGTTACGAGGACGCAGCACTCGACCAGCTCGGCCTCGTCGTCAGTGTCGCCAT
CAATTTGATGATCGTGCTGGTCTTCCTGCCTCTGATCCTTTTGATGTGGGGGTTCCAGCCCGGCGACATCCAGGCCTGGG
CCTACAAGCTGGCGACCGGGATCACCATCGGCTCGGTGACGATTTCGGTCACTGGCATCCTTTCGGGCATCATCGTCTTC
ATCATCGGCTATTTCCTGACCCGCTGGTTCCAGGGCTGGCTCGACGGGTCGGTGATGGCGCGCGGCAAGGTCGACACCGG
CGTGCGCAATTCGATCCGGCTGGCGGTCGGCTATGCCGGCGTCGCCGTGGCGGGGCTGGTCGGCATATCGGCGGCGGGTA
TCGACCTCTCCAGCCTGGCGCTGGTCGCCGGTGCGCTTTCCCTCGGCATCGGCTTTGGCCTGCAGAATGTCGTGTCGAAT
TTCGTCTCCGGCCTGATCCTGCTGGCCGAGCGGCCGTTCAAGGTCGGCGATTGGATCGTCGCCGGCGACGTCAGCGGTAC
GGTCAAGAAGATCAGCGTGCGCGCCACCGAGATCGAGACTTTCCAGCGGCAGTCGGTGATCCTGCCCAATTCGAACCTGA
TCAACAACGCCGTCGGCAACTGGACGCATCGCAACAAGCTCGGCCGCATCGACATCAAGGTCGGCGTCGCCTATGGCAGC
GACGTCAAGCAGGTGCATGCCATCCTGCTGGAAATCGCCCGCAGCCACCCGATGGTGCTGAAGAACCCCGAACCCTTCGT
GCTGTTTTCCAATTTTGGACCAGCGGCACTTGAGTTCGAAATCCGTGTGTTCCTGGCCGATGTGACGAACGGCAACATCG
TGCAGAACGACGTCCGCTTCGCCGTGCTGGAAAAATTCAGCGACCAGCATATCGAGATCCCGTCGACACCGCGTGCCGTC
GTCGAGGTGCACAAGCCCAAGGCCTGGCCGACAGACGACGACAAGATCGAGGCCGACTTCGTCGAACAGGAACAGGCGAA
GGCGGACGCCGCGGCCGAAGCGAAAAAGCTCGCCAAATCGGGACGCAAGCTGCGCAAGCCGGATCCCGACTAG

Upstream 100 bases:

>100_bases
CGATGCGGCAGACTATAGAGCAGGCCGCATCCGCAAAGCCAATATTGCGCCATGGCCTTTCGACTGGCCGACGCCGCGAT
GCGACGGCTTGCCCCACGAT

Downstream 100 bases:

>100_bases
GGCCGGTTCCCGTCAGCTTCTCGCGACCAACGCCGTCAATGCCGCGCGCAGATCGGTATCGCCGCGCCTGACTTCGCCAA
CGCCGTGCTCGACATCGATG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 850; Mature: 849

Protein sequence:

>850_residues
MAVFIKDRLLNALAAGESPIVMLLRLLRLVLVFTLVISASPSFEAMAQALGQGSAGLITDQQKVIQGLTAKTDDLEKKIQ
QNGEDDASLVDIRLQLEDLSRAALTSALVFRPRLTDINNRIEVLGPPPAAGQPQEPDIVTGERQALASEKAEINAVVASA
QNLSIRISGLIDKIGNMRSEQFRNFLTKRYALSEALSPQVFSDARDEYANFYKAVSSWLSFAFKFKFQAILAATFVALGL
ALVLLVGGRRLFGRIFEADPAVEDPSYLSRLSVAFWSTLLPTLAVGVFLASTVFFFNYYNVLRGDIGVFLNALAAVIAVV
FCVNRLTNAALEPRLPNWRLIPVQTGPARWLVRLTTAMAVVIGVNNFLSIVNDKMGSPLSLTIARSFVATIVVGIILILM
AMLRPFKARDGSWRPWPAWLRYLALALGLFTIAAALLGYIGLALFVSLQVVVTGTALITAYIGFLSAQAIGEEGAFANTT
VGRWLSANSSYEDAALDQLGLVVSVAINLMIVLVFLPLILLMWGFQPGDIQAWAYKLATGITIGSVTISVTGILSGIIVF
IIGYFLTRWFQGWLDGSVMARGKVDTGVRNSIRLAVGYAGVAVAGLVGISAAGIDLSSLALVAGALSLGIGFGLQNVVSN
FVSGLILLAERPFKVGDWIVAGDVSGTVKKISVRATEIETFQRQSVILPNSNLINNAVGNWTHRNKLGRIDIKVGVAYGS
DVKQVHAILLEIARSHPMVLKNPEPFVLFSNFGPAALEFEIRVFLADVTNGNIVQNDVRFAVLEKFSDQHIEIPSTPRAV
VEVHKPKAWPTDDDKIEADFVEQEQAKADAAAEAKKLAKSGRKLRKPDPD

Sequences:

>Translated_850_residues
MAVFIKDRLLNALAAGESPIVMLLRLLRLVLVFTLVISASPSFEAMAQALGQGSAGLITDQQKVIQGLTAKTDDLEKKIQ
QNGEDDASLVDIRLQLEDLSRAALTSALVFRPRLTDINNRIEVLGPPPAAGQPQEPDIVTGERQALASEKAEINAVVASA
QNLSIRISGLIDKIGNMRSEQFRNFLTKRYALSEALSPQVFSDARDEYANFYKAVSSWLSFAFKFKFQAILAATFVALGL
ALVLLVGGRRLFGRIFEADPAVEDPSYLSRLSVAFWSTLLPTLAVGVFLASTVFFFNYYNVLRGDIGVFLNALAAVIAVV
FCVNRLTNAALEPRLPNWRLIPVQTGPARWLVRLTTAMAVVIGVNNFLSIVNDKMGSPLSLTIARSFVATIVVGIILILM
AMLRPFKARDGSWRPWPAWLRYLALALGLFTIAAALLGYIGLALFVSLQVVVTGTALITAYIGFLSAQAIGEEGAFANTT
VGRWLSANSSYEDAALDQLGLVVSVAINLMIVLVFLPLILLMWGFQPGDIQAWAYKLATGITIGSVTISVTGILSGIIVF
IIGYFLTRWFQGWLDGSVMARGKVDTGVRNSIRLAVGYAGVAVAGLVGISAAGIDLSSLALVAGALSLGIGFGLQNVVSN
FVSGLILLAERPFKVGDWIVAGDVSGTVKKISVRATEIETFQRQSVILPNSNLINNAVGNWTHRNKLGRIDIKVGVAYGS
DVKQVHAILLEIARSHPMVLKNPEPFVLFSNFGPAALEFEIRVFLADVTNGNIVQNDVRFAVLEKFSDQHIEIPSTPRAV
VEVHKPKAWPTDDDKIEADFVEQEQAKADAAAEAKKLAKSGRKLRKPDPD
>Mature_849_residues
AVFIKDRLLNALAAGESPIVMLLRLLRLVLVFTLVISASPSFEAMAQALGQGSAGLITDQQKVIQGLTAKTDDLEKKIQQ
NGEDDASLVDIRLQLEDLSRAALTSALVFRPRLTDINNRIEVLGPPPAAGQPQEPDIVTGERQALASEKAEINAVVASAQ
NLSIRISGLIDKIGNMRSEQFRNFLTKRYALSEALSPQVFSDARDEYANFYKAVSSWLSFAFKFKFQAILAATFVALGLA
LVLLVGGRRLFGRIFEADPAVEDPSYLSRLSVAFWSTLLPTLAVGVFLASTVFFFNYYNVLRGDIGVFLNALAAVIAVVF
CVNRLTNAALEPRLPNWRLIPVQTGPARWLVRLTTAMAVVIGVNNFLSIVNDKMGSPLSLTIARSFVATIVVGIILILMA
MLRPFKARDGSWRPWPAWLRYLALALGLFTIAAALLGYIGLALFVSLQVVVTGTALITAYIGFLSAQAIGEEGAFANTTV
GRWLSANSSYEDAALDQLGLVVSVAINLMIVLVFLPLILLMWGFQPGDIQAWAYKLATGITIGSVTISVTGILSGIIVFI
IGYFLTRWFQGWLDGSVMARGKVDTGVRNSIRLAVGYAGVAVAGLVGISAAGIDLSSLALVAGALSLGIGFGLQNVVSNF
VSGLILLAERPFKVGDWIVAGDVSGTVKKISVRATEIETFQRQSVILPNSNLINNAVGNWTHRNKLGRIDIKVGVAYGSD
VKQVHAILLEIARSHPMVLKNPEPFVLFSNFGPAALEFEIRVFLADVTNGNIVQNDVRFAVLEKFSDQHIEIPSTPRAVV
EVHKPKAWPTDDDKIEADFVEQEQAKADAAAEAKKLAKSGRKLRKPDPD

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=263, Percent_Identity=35.7414448669202, Blast_Score=167, Evalue=3e-42,
Organism=Escherichia coli, GI2367355, Length=345, Percent_Identity=28.1159420289855, Blast_Score=149, Evalue=1e-36,
Organism=Escherichia coli, GI1789291, Length=237, Percent_Identity=28.6919831223629, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787591, Length=160, Percent_Identity=32.5, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 91947; Mature: 91816

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFIKDRLLNALAAGESPIVMLLRLLRLVLVFTLVISASPSFEAMAQALGQGSAGLITD
CEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
QQKVIQGLTAKTDDLEKKIQQNGEDDASLVDIRLQLEDLSRAALTSALVFRPRLTDINNR
HHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCC
IEVLGPPPAAGQPQEPDIVTGERQALASEKAEINAVVASAQNLSIRISGLIDKIGNMRSE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCHHHH
QFRNFLTKRYALSEALSPQVFSDARDEYANFYKAVSSWLSFAFKFKFQAILAATFVALGL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVLLVGGRRLFGRIFEADPAVEDPSYLSRLSVAFWSTLLPTLAVGVFLASTVFFFNYYN
HHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLRGDIGVFLNALAAVIAVVFCVNRLTNAALEPRLPNWRLIPVQTGPARWLVRLTTAMAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
VIGVNNFLSIVNDKMGSPLSLTIARSFVATIVVGIILILMAMLRPFKARDGSWRPWPAWL
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RYLALALGLFTIAAALLGYIGLALFVSLQVVVTGTALITAYIGFLSAQAIGEEGAFANTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
VGRWLSANSSYEDAALDQLGLVVSVAINLMIVLVFLPLILLMWGFQPGDIQAWAYKLATG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
ITIGSVTISVTGILSGIIVFIIGYFLTRWFQGWLDGSVMARGKVDTGVRNSIRLAVGYAG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEHHH
VAVAGLVGISAAGIDLSSLALVAGALSLGIGFGLQNVVSNFVSGLILLAERPFKVGDWIV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
AGDVSGTVKKISVRATEIETFQRQSVILPNSNLINNAVGNWTHRNKLGRIDIKVGVAYGS
ECCCCCCHHEEEEEHHHHHHHHHCEEECCCCCHHHHCCCCCHHHCCCCEEEEEEEEECCC
DVKQVHAILLEIARSHPMVLKNPEPFVLFSNFGPAALEFEIRVFLADVTNGNIVQNDVRF
CHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCCEEEEEEEEEEEECCCCCEEHHHHHH
AVLEKFSDQHIEIPSTPRAVVEVHKPKAWPTDDDKIEADFVEQEQAKADAAAEAKKLAKS
HHHHHCCCCCEECCCCCHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GRKLRKPDPD
CCCCCCCCCC
>Mature Secondary Structure 
AVFIKDRLLNALAAGESPIVMLLRLLRLVLVFTLVISASPSFEAMAQALGQGSAGLITD
EEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
QQKVIQGLTAKTDDLEKKIQQNGEDDASLVDIRLQLEDLSRAALTSALVFRPRLTDINNR
HHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCC
IEVLGPPPAAGQPQEPDIVTGERQALASEKAEINAVVASAQNLSIRISGLIDKIGNMRSE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCHHHH
QFRNFLTKRYALSEALSPQVFSDARDEYANFYKAVSSWLSFAFKFKFQAILAATFVALGL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVLLVGGRRLFGRIFEADPAVEDPSYLSRLSVAFWSTLLPTLAVGVFLASTVFFFNYYN
HHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLRGDIGVFLNALAAVIAVVFCVNRLTNAALEPRLPNWRLIPVQTGPARWLVRLTTAMAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
VIGVNNFLSIVNDKMGSPLSLTIARSFVATIVVGIILILMAMLRPFKARDGSWRPWPAWL
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RYLALALGLFTIAAALLGYIGLALFVSLQVVVTGTALITAYIGFLSAQAIGEEGAFANTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
VGRWLSANSSYEDAALDQLGLVVSVAINLMIVLVFLPLILLMWGFQPGDIQAWAYKLATG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
ITIGSVTISVTGILSGIIVFIIGYFLTRWFQGWLDGSVMARGKVDTGVRNSIRLAVGYAG
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEHHH
VAVAGLVGISAAGIDLSSLALVAGALSLGIGFGLQNVVSNFVSGLILLAERPFKVGDWIV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
AGDVSGTVKKISVRATEIETFQRQSVILPNSNLINNAVGNWTHRNKLGRIDIKVGVAYGS
ECCCCCCHHEEEEEHHHHHHHHHCEEECCCCCHHHHCCCCCHHHCCCCEEEEEEEEECCC
DVKQVHAILLEIARSHPMVLKNPEPFVLFSNFGPAALEFEIRVFLADVTNGNIVQNDVRF
CHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCCEEEEEEEEEEEECCCCCEEHHHHHH
AVLEKFSDQHIEIPSTPRAVVEVHKPKAWPTDDDKIEADFVEQEQAKADAAAEAKKLAKS
HHHHHCCCCCEECCCCCHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GRKLRKPDPD
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]