Click here to switch to the map view.

The map label for this gene is tgt

Identifier: 13470901

GI number: 13470901

Start: 569926

End: 571056

Strand: Reverse

Name: tgt

Synonym: mll0721

Alternate gene names: 13470901

Gene position: 571056-569926 (Counterclockwise)

Preceding gene: 13470902

Following gene: 13470899

Centisome position: 8.12

GC content: 65.61

Gene sequence:

>1131_bases
ATGGCTAAGCCGTTCAGCTTCAAGGTCCTTGCCACCGACGGCAGGGCGCGGCGCGGTATCATCGACATGCCGCGCGGCGA
AATCCGCACGCCTGCCTTCATGCCGGTCGGCACCGGCGGCACCGTGAAGACCATGTATATGGACCAGGTGCGCGGCGTCG
GCGCCGACATCATCCTGGGCAACACCTATCACCTGATGCTGCGGCCCGGCGCCGAGCGTGTCGCGCGACTTGGCGGATTG
CATGAATTCGCCCGCTGGCCGCATCCGATCCTGACCGACAGCGGCGGTTTCCAGGTGATGTCGCTGTCGAAGCTCAGGAA
ACTGACCGAAAAGGGCGTCACCTTCCGCTCGCATATCGATGGCGCGCCTTACGAAATGTCGCCGGAGCGCTCGATCGAGA
TCCAGAGCCTGCTCGATTCCGACATCCAGATGCAGCTCGACGAATGCACGGCGCTGCCGGCCGAGCTCAAGGAGATCGAG
CGCGCCATGGAACTGTCGCTGCGTTGGGCTGAGCGCTGCAAGACGGCGTTCGGCGACCAGCCGGGCAAGGCGATGTTCGG
CATCGTACAGGGCGGCGACAATGCGGCACTTCGGGTGCGCTCGGCGCAGGCGCTGAGCGCGATGGGCTTGAAGGGCTATG
CGGTCGGCGGCCTGGCGGTCGGCGAGCCGCAAGCGGTGATGCTCGAAATGCTCGACATCACCTGTCCCGAACTGCCCGCG
GACAAGCCGCGCTATCTCATGGGCGTCGGTACGCCCGACGACATCCTGAAGTCCGTGGCGCGCGGCATCGATATGTTCGA
CTGCGTGATGCCGACGCGGGCCGGCCGGCACGGCCTGGCCTACACCAGGCGCGGCAAGGTCAATCTGCGCAACGCCCGCC
ATGCCGACGATCCGCGCCCGCTCGACGAGGAAAGCGATTGTCCGGCGGCACGCGATTATTCGCGCGCCTATCTGCACCAT
CTGGTGCGCTCGCAGGAGGCGCTGGGCGCGATGCTGCTCACCTGGAACAACCTGTCCTACTATCAAAAGCTGATGCAGGA
CATTCGCGCCGCGATCGAGACCCAAACCTTCGAGGCGCGTGGGGCCGAGATCACAGAGGGCTGGGCGAGGGGCGATATCC
CGGTCCTGTAG

Upstream 100 bases:

>100_bases
GCTGTGCCGCCCTGGCCCAAATTCATGCGCGGCTGCATCCAATATCGGCAATCGCTCGACAGACAGGCGCCTGACGCGCC
CATCCACGACAAGGAATTCA

Downstream 100 bases:

>100_bases
AACCTTAAGTACTCAGCGAGTTGGATGCGCGCAGGCTGCAGCCGGTGATCTGCAACGTGCCGTCGGGCTGCTGCTGCAGC
GTATAGACCGCCTCGTAATC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAKPFSFKVLATDGRARRGIIDMPRGEIRTPAFMPVGTGGTVKTMYMDQVRGVGADIILGNTYHLMLRPGAERVARLGGL
HEFARWPHPILTDSGGFQVMSLSKLRKLTEKGVTFRSHIDGAPYEMSPERSIEIQSLLDSDIQMQLDECTALPAELKEIE
RAMELSLRWAERCKTAFGDQPGKAMFGIVQGGDNAALRVRSAQALSAMGLKGYAVGGLAVGEPQAVMLEMLDITCPELPA
DKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAYTRRGKVNLRNARHADDPRPLDEESDCPAARDYSRAYLHH
LVRSQEALGAMLLTWNNLSYYQKLMQDIRAAIETQTFEARGAEITEGWARGDIPVL

Sequences:

>Translated_376_residues
MAKPFSFKVLATDGRARRGIIDMPRGEIRTPAFMPVGTGGTVKTMYMDQVRGVGADIILGNTYHLMLRPGAERVARLGGL
HEFARWPHPILTDSGGFQVMSLSKLRKLTEKGVTFRSHIDGAPYEMSPERSIEIQSLLDSDIQMQLDECTALPAELKEIE
RAMELSLRWAERCKTAFGDQPGKAMFGIVQGGDNAALRVRSAQALSAMGLKGYAVGGLAVGEPQAVMLEMLDITCPELPA
DKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAYTRRGKVNLRNARHADDPRPLDEESDCPAARDYSRAYLHH
LVRSQEALGAMLLTWNNLSYYQKLMQDIRAAIETQTFEARGAEITEGWARGDIPVL
>Mature_375_residues
AKPFSFKVLATDGRARRGIIDMPRGEIRTPAFMPVGTGGTVKTMYMDQVRGVGADIILGNTYHLMLRPGAERVARLGGLH
EFARWPHPILTDSGGFQVMSLSKLRKLTEKGVTFRSHIDGAPYEMSPERSIEIQSLLDSDIQMQLDECTALPAELKEIER
AMELSLRWAERCKTAFGDQPGKAMFGIVQGGDNAALRVRSAQALSAMGLKGYAVGGLAVGEPQAVMLEMLDITCPELPAD
KPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAYTRRGKVNLRNARHADDPRPLDEESDCPAARDYSRAYLHHL
VRSQEALGAMLLTWNNLSYYQKLMQDIRAAIETQTFEARGAEITEGWARGDIPVL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=343, Percent_Identity=43.731778425656, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI13375872, Length=280, Percent_Identity=25, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI1786607, Length=355, Percent_Identity=55.2112676056338, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17544680, Length=353, Percent_Identity=42.7762039660057, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI25146502, Length=137, Percent_Identity=30.6569343065693, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920510, Length=356, Percent_Identity=44.3820224719101, Blast_Score=318, Evalue=6e-87,
Organism=Drosophila melanogaster, GI21355311, Length=251, Percent_Identity=23.9043824701195, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_RHILO (Q98M57)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102470.1
- ProteinModelPortal:   Q98M57
- SMR:   Q98M57
- GeneID:   1225133
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll0721
- NMPDR:   fig|266835.1.peg.575
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41490; Mature: 41359

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPFSFKVLATDGRARRGIIDMPRGEIRTPAFMPVGTGGTVKTMYMDQVRGVGADIILG
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEHHHHHHHCCCCCEEEEC
NTYHLMLRPGAERVARLGGLHEFARWPHPILTDSGGFQVMSLSKLRKLTEKGVTFRSHID
CEEEEEECCCHHHHHHHCCHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCEEEHHCC
GAPYEMSPERSIEIQSLLDSDIQMQLDECTALPAELKEIERAMELSLRWAERCKTAFGDQ
CCCCCCCCCCCCHHHHHHCCHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGKAMFGIVQGGDNAALRVRSAQALSAMGLKGYAVGGLAVGEPQAVMLEMLDITCPELPA
CCCEEEEEEECCCCCEEEEEHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHCCCCCCCC
DKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAYTRRGKVNLRNARHADDPRP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCEEECCCCCCCCCCC
LDEESDCPAARDYSRAYLHHLVRSQEALGAMLLTWNNLSYYQKLMQDIRAAIETQTFEAR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
GAEITEGWARGDIPVL
CCCHHCCCCCCCCCCC
>Mature Secondary Structure 
AKPFSFKVLATDGRARRGIIDMPRGEIRTPAFMPVGTGGTVKTMYMDQVRGVGADIILG
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEHHHHHHHCCCCCEEEEC
NTYHLMLRPGAERVARLGGLHEFARWPHPILTDSGGFQVMSLSKLRKLTEKGVTFRSHID
CEEEEEECCCHHHHHHHCCHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCEEEHHCC
GAPYEMSPERSIEIQSLLDSDIQMQLDECTALPAELKEIERAMELSLRWAERCKTAFGDQ
CCCCCCCCCCCCHHHHHHCCHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGKAMFGIVQGGDNAALRVRSAQALSAMGLKGYAVGGLAVGEPQAVMLEMLDITCPELPA
CCCEEEEEEECCCCCEEEEEHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHCCCCCCCC
DKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRAGRHGLAYTRRGKVNLRNARHADDPRP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCEEECCCCCCCCCCC
LDEESDCPAARDYSRAYLHHLVRSQEALGAMLLTWNNLSYYQKLMQDIRAAIETQTFEAR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
GAEITEGWARGDIPVL
CCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968