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The map label for this gene is otsA [H]

Identifier: 13470877

GI number: 13470877

Start: 541391

End: 542953

Strand: Reverse

Name: otsA [H]

Synonym: mll0691

Alternate gene names: 13470877

Gene position: 542953-541391 (Counterclockwise)

Preceding gene: 13470878

Following gene: 13470876

Centisome position: 7.72

GC content: 61.16

Gene sequence:

>1563_bases
GTGCAATGGTCGCCCGAAACGCTGCGTCACATCCTGGCGACCGAACTTCCCGATGCCCAGGTGATCGTCGTCTCAAACCG
TGAACCGTATATCCACAACCGCGAGGGCGACGACATCCAGCTGGTCGTACCCGCCAGCGGGCTCGTCTCCGCCCTGGAGC
CGATCACGCGTGCCTGCGCGGGCACCTGGATCGCCTATGGCGGTGGTTCGGCCGACAAGCTGGTTGTCGACAAGAACGAC
CGGATCGAGGTGCCGCCGGACAACCCTTCCTACACGCTGCGCCGTGTCTGGCTGAGCGAGGAAGAACAGCAAGGCTATTA
TCTCGGCTTTGCCAATGAAGGCCTCTGGCCGCTGTGCCATATCGCCTTCACCCGGCCGATATTCCGCGCCTCGGATTGGG
AGGCCTACGAGGCGGTCAACCGCAAATTCGCCGACACCGTCGTCGCGGAAGCCCGCAACGAACGGCCCATCGTGCTGGTC
CAGGATTATCATTTCGCGCTGCTGCCGCGAATGATCCGTGAACGGCTGCCGGAGGCGATCATCATCACCTTCTGGCATAT
TCCGTGGCCGAATTCCGAGGTGTTCAGCATCTGCCCATGGCGCGAAAAGATTCTCGACGGCCTGCTCGGCAGTTCTATCG
TCGGTTTCCACACCCAGTTCCATTGCAACAACTTCATCGACAGCGTCGACCGTTTCCTGGAAAGCCGGATCGAGCGCGAG
GATTCGGCCATTTCCTATGGCGGCCAGATCAGCCTGGTCCATGCCTATCCGATCTCAATCGAGTGGCCGCCGGCGCAATT
GGGCAAACTGCCTGACGTGGCGCAATGCCGCCGGCAGGTTCGCGAGAAATTCGGCCTGGCCGAAAATGTCAAGCTGTGCG
TGGGCGTCGAACGTCTCGACTACACCAAAGGCATCCTCGATCGCTTCAACGCGCTCGACGAACTGCTGACGCTTCATCCC
GAATGGATCGGCAAGGTGGCGTTCCTGCAGATCGCTGCGCCCAGCCGCGGCACGTTGCCTGCCTATCAACAATTGTACGA
GGAGTGCCTGCGCCATGCCGAGGATCTCAACCAGCGCTATGGTCGCGAAGGCTACACACCAGTCCTGATGATAACGGAAC
ATCACTCGCAGCAGCAGGTCTACGAAATCTACCGTGCAGCCGACGTTTGCATGGTCACCAGTCTGCATGACGGCATGAAC
CTGGTTGCCAAGGAGTTTGTCGCGGCGCGTGAGGACGAACAGGGCGTGCTGTTGCTCAGCATGTTCGCCGGCGCCTCCAA
AGAGCTGCTGGACGCACTGATCGTCAATCCCTATGACGCGGCTATGATGGGAGATGCCTTGCTGCAGGCCTTGACCATGT
CGCAGGAAGAGCAAAGCCAGCGCATGCGACGCATGCGCGAGATCGTACGCGACAACAACGTCTATCGGTGGGCCGGCAGC
ATGCTTCTCGATGCGGCGCGCTTGCGCAAGCGCGACGCGGTCGATCGCGCCGTCAGCGCCGCTCCAGCGGCCCCAGCCAA
TGTCATATCGCTGCTCGATCGCAGACGCGTGGCGGCGCTATGA

Upstream 100 bases:

>100_bases
GGCGCGCAAGGGCCGCAGGCTGGCCGTTGCGGGGGTTGACCCTGCTCGCGGCTCATTGCCGGAATTCGAGAAGAACGGGC
AGTCGGCGGCCGCGGCACTG

Downstream 100 bases:

>100_bases
TCTCTCTCTCGCCAAACCTCGCCGTGCCTGTCGTTCCGCAAGGGCCATGCAGCCTGTTCCTCGATATCGACGGCACGCTT
CTCGAACATGCCGCGCATCC

Product: trehalose-6-phosphate phosphatase

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MQWSPETLRHILATELPDAQVIVVSNREPYIHNREGDDIQLVVPASGLVSALEPITRACAGTWIAYGGGSADKLVVDKND
RIEVPPDNPSYTLRRVWLSEEEQQGYYLGFANEGLWPLCHIAFTRPIFRASDWEAYEAVNRKFADTVVAEARNERPIVLV
QDYHFALLPRMIRERLPEAIIITFWHIPWPNSEVFSICPWREKILDGLLGSSIVGFHTQFHCNNFIDSVDRFLESRIERE
DSAISYGGQISLVHAYPISIEWPPAQLGKLPDVAQCRRQVREKFGLAENVKLCVGVERLDYTKGILDRFNALDELLTLHP
EWIGKVAFLQIAAPSRGTLPAYQQLYEECLRHAEDLNQRYGREGYTPVLMITEHHSQQQVYEIYRAADVCMVTSLHDGMN
LVAKEFVAAREDEQGVLLLSMFAGASKELLDALIVNPYDAAMMGDALLQALTMSQEEQSQRMRRMREIVRDNNVYRWAGS
MLLDAARLRKRDAVDRAVSAAPAAPANVISLLDRRRVAAL

Sequences:

>Translated_520_residues
MQWSPETLRHILATELPDAQVIVVSNREPYIHNREGDDIQLVVPASGLVSALEPITRACAGTWIAYGGGSADKLVVDKND
RIEVPPDNPSYTLRRVWLSEEEQQGYYLGFANEGLWPLCHIAFTRPIFRASDWEAYEAVNRKFADTVVAEARNERPIVLV
QDYHFALLPRMIRERLPEAIIITFWHIPWPNSEVFSICPWREKILDGLLGSSIVGFHTQFHCNNFIDSVDRFLESRIERE
DSAISYGGQISLVHAYPISIEWPPAQLGKLPDVAQCRRQVREKFGLAENVKLCVGVERLDYTKGILDRFNALDELLTLHP
EWIGKVAFLQIAAPSRGTLPAYQQLYEECLRHAEDLNQRYGREGYTPVLMITEHHSQQQVYEIYRAADVCMVTSLHDGMN
LVAKEFVAAREDEQGVLLLSMFAGASKELLDALIVNPYDAAMMGDALLQALTMSQEEQSQRMRRMREIVRDNNVYRWAGS
MLLDAARLRKRDAVDRAVSAAPAAPANVISLLDRRRVAAL
>Mature_520_residues
MQWSPETLRHILATELPDAQVIVVSNREPYIHNREGDDIQLVVPASGLVSALEPITRACAGTWIAYGGGSADKLVVDKND
RIEVPPDNPSYTLRRVWLSEEEQQGYYLGFANEGLWPLCHIAFTRPIFRASDWEAYEAVNRKFADTVVAEARNERPIVLV
QDYHFALLPRMIRERLPEAIIITFWHIPWPNSEVFSICPWREKILDGLLGSSIVGFHTQFHCNNFIDSVDRFLESRIERE
DSAISYGGQISLVHAYPISIEWPPAQLGKLPDVAQCRRQVREKFGLAENVKLCVGVERLDYTKGILDRFNALDELLTLHP
EWIGKVAFLQIAAPSRGTLPAYQQLYEECLRHAEDLNQRYGREGYTPVLMITEHHSQQQVYEIYRAADVCMVTSLHDGMN
LVAKEFVAAREDEQGVLLLSMFAGASKELLDALIVNPYDAAMMGDALLQALTMSQEEQSQRMRRMREIVRDNNVYRWAGS
MLLDAARLRKRDAVDRAVSAAPAAPANVISLLDRRRVAAL

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=491, Percent_Identity=31.9755600814664, Blast_Score=230, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI71987012, Length=429, Percent_Identity=27.7389277389277, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115533558, Length=369, Percent_Identity=28.9972899728997, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI115533560, Length=369, Percent_Identity=28.9972899728997, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319602, Length=478, Percent_Identity=31.3807531380753, Blast_Score=253, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6323917, Length=382, Percent_Identity=29.3193717277487, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6323537, Length=397, Percent_Identity=28.463476070529, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6320279, Length=415, Percent_Identity=27.2289156626506, Blast_Score=163, Evalue=8e-41,
Organism=Drosophila melanogaster, GI19920676, Length=501, Percent_Identity=33.3333333333333, Blast_Score=266, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 58997; Mature: 58997

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWSPETLRHILATELPDAQVIVVSNREPYIHNREGDDIQLVVPASGLVSALEPITRACA
CCCCHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCEEEEECCHHHHHHHHHHHHHHH
GTWIAYGGGSADKLVVDKNDRIEVPPDNPSYTLRRVWLSEEEQQGYYLGFANEGLWPLCH
CCEEEECCCCCCEEEECCCCEEECCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCHHHH
IAFTRPIFRASDWEAYEAVNRKFADTVVAEARNERPIVLVQDYHFALLPRMIRERLPEAI
HHHHCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCEE
IITFWHIPWPNSEVFSICPWREKILDGLLGSSIVGFHTQFHCNNFIDSVDRFLESRIERE
EEEEEECCCCCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DSAISYGGQISLVHAYPISIEWPPAQLGKLPDVAQCRRQVREKFGLAENVKLCVGVERLD
CCCCCCCCEEEEEEEEEEEEECCHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHH
YTKGILDRFNALDELLTLHPEWIGKVAFLQIAAPSRGTLPAYQQLYEECLRHAEDLNQRY
HHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
GREGYTPVLMITEHHSQQQVYEIYRAADVCMVTSLHDGMNLVAKEFVAAREDEQGVLLLS
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
MFAGASKELLDALIVNPYDAAMMGDALLQALTMSQEEQSQRMRRMREIVRDNNVYRWAGS
ECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEHHH
MLLDAARLRKRDAVDRAVSAAPAAPANVISLLDRRRVAAL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQWSPETLRHILATELPDAQVIVVSNREPYIHNREGDDIQLVVPASGLVSALEPITRACA
CCCCHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCEEEEECCHHHHHHHHHHHHHHH
GTWIAYGGGSADKLVVDKNDRIEVPPDNPSYTLRRVWLSEEEQQGYYLGFANEGLWPLCH
CCEEEECCCCCCEEEECCCCEEECCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCHHHH
IAFTRPIFRASDWEAYEAVNRKFADTVVAEARNERPIVLVQDYHFALLPRMIRERLPEAI
HHHHCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCEE
IITFWHIPWPNSEVFSICPWREKILDGLLGSSIVGFHTQFHCNNFIDSVDRFLESRIERE
EEEEEECCCCCCCCEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DSAISYGGQISLVHAYPISIEWPPAQLGKLPDVAQCRRQVREKFGLAENVKLCVGVERLD
CCCCCCCCEEEEEEEEEEEEECCHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHH
YTKGILDRFNALDELLTLHPEWIGKVAFLQIAAPSRGTLPAYQQLYEECLRHAEDLNQRY
HHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
GREGYTPVLMITEHHSQQQVYEIYRAADVCMVTSLHDGMNLVAKEFVAAREDEQGVLLLS
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
MFAGASKELLDALIVNPYDAAMMGDALLQALTMSQEEQSQRMRRMREIVRDNNVYRWAGS
ECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEHHH
MLLDAARLRKRDAVDRAVSAAPAAPANVISLLDRRRVAAL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA