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The map label for this gene is mazG [H]

Identifier: 13470638

GI number: 13470638

Start: 291212

End: 292036

Strand: Reverse

Name: mazG [H]

Synonym: mll0404

Alternate gene names: 13470638

Gene position: 292036-291212 (Counterclockwise)

Preceding gene: 13470639

Following gene: 13470629

Centisome position: 4.15

GC content: 62.67

Gene sequence:

>825_bases
ATGACGCCATCCAAAGACATTTCACGCCTGATCGAGATCATGGCGGCGCTGCGTGCGCCCAAGACCGGCTGCCCCTGGGA
CATCGAGCAGGATTTCTCGACCATCGCGCCCTATACGATCGAGGAAGCGTATGAGGTGGCGGACGCCATCGCGCGCGGTG
ACCTCAACGATCTGCGCGATGAACTCGGCGACCTGCTGCTGCAGGTCGTCTACCACGCGCAAATGGCCGAGGAAGCGGGC
GAATTCGCCTTCCCGGATGTCGTCCAGGCCATCACCACCAAGATGATCCGGCGCCACCCGCATGTCTTCGGCGACGAAGA
AGCCCGCGGCGCCGGCATGGCCAAGGGCATGTGGGAGAAGATCAAGGCCGAGGAGAAGGCGGAGAAGCGGCAAGCCCGTC
TCGCCCGCGGCCTCGACCCCGAAGACAATGGTAAGGGATTTCTGGACAGTGTTCCGGTCGCCCTGCCCGCTTTGACGCGG
GCGCTCAAACTTCAGGAGAAGGCTGCCCGTGTCGGCTTCGACTGGAGCGAGGCAGCCCCCATCCTCGACAAGATCGAGGA
GGAGATCGGCGAATTGCGTGAGGCTCTGGCGAGGGGCGAGACGGCCTCGATCAAGGACGAGTTCGGCGACATGCTGTTCG
CCGTCGTTAATCTCGGCCGTCATCTCAAGCTCGATTCGGAAGCCGCGCTGAGCGGCACCAACGAGAAATTCCGGTCGCGC
TTCCACTATGTCGAGCAGGCTCTTGAGAAGTCCGGCAACACACTGGAGAAGGCCACCCTGGACGAGATGGAAGCGCTCTG
GCAGGAAGCGAAGAGCGCGAAATAG

Upstream 100 bases:

>100_bases
TCGTCTCCGTGCTCGACTGAAGCGGCTCAGATGTGGACAAGGCTCCCTCGCCGGCTGACAGCATTGGCCGCGCGTCGGGC
CGCAGGATAGCATCCGCCGC

Downstream 100 bases:

>100_bases
GGCCGATAGACCGACAATTCCGGAGGGAAAACCGCCTTTTTACTGGAAATTCTTCGGCTTACCCGTTGTTTTTACGACGC
AATCGGCTTTCGATTTCTTC

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTPSKDISRLIEIMAALRAPKTGCPWDIEQDFSTIAPYTIEEAYEVADAIARGDLNDLRDELGDLLLQVVYHAQMAEEAG
EFAFPDVVQAITTKMIRRHPHVFGDEEARGAGMAKGMWEKIKAEEKAEKRQARLARGLDPEDNGKGFLDSVPVALPALTR
ALKLQEKAARVGFDWSEAAPILDKIEEEIGELREALARGETASIKDEFGDMLFAVVNLGRHLKLDSEAALSGTNEKFRSR
FHYVEQALEKSGNTLEKATLDEMEALWQEAKSAK

Sequences:

>Translated_274_residues
MTPSKDISRLIEIMAALRAPKTGCPWDIEQDFSTIAPYTIEEAYEVADAIARGDLNDLRDELGDLLLQVVYHAQMAEEAG
EFAFPDVVQAITTKMIRRHPHVFGDEEARGAGMAKGMWEKIKAEEKAEKRQARLARGLDPEDNGKGFLDSVPVALPALTR
ALKLQEKAARVGFDWSEAAPILDKIEEEIGELREALARGETASIKDEFGDMLFAVVNLGRHLKLDSEAALSGTNEKFRSR
FHYVEQALEKSGNTLEKATLDEMEALWQEAKSAK
>Mature_273_residues
TPSKDISRLIEIMAALRAPKTGCPWDIEQDFSTIAPYTIEEAYEVADAIARGDLNDLRDELGDLLLQVVYHAQMAEEAGE
FAFPDVVQAITTKMIRRHPHVFGDEEARGAGMAKGMWEKIKAEEKAEKRQARLARGLDPEDNGKGFLDSVPVALPALTRA
LKLQEKAARVGFDWSEAAPILDKIEEEIGELREALARGETASIKDEFGDMLFAVVNLGRHLKLDSEAALSGTNEKFRSRF
HYVEQALEKSGNTLEKATLDEMEALWQEAKSAK

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=269, Percent_Identity=49.0706319702602, Blast_Score=258, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 30458; Mature: 30327

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSKDISRLIEIMAALRAPKTGCPWDIEQDFSTIAPYTIEEAYEVADAIARGDLNDLRD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHH
ELGDLLLQVVYHAQMAEEAGEFAFPDVVQAITTKMIRRHPHVFGDEEARGAGMAKGMWEK
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHH
IKAEEKAEKRQARLARGLDPEDNGKGFLDSVPVALPALTRALKLQEKAARVGFDWSEAAP
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ILDKIEEEIGELREALARGETASIKDEFGDMLFAVVNLGRHLKLDSEAALSGTNEKFRSR
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHH
FHYVEQALEKSGNTLEKATLDEMEALWQEAKSAK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPSKDISRLIEIMAALRAPKTGCPWDIEQDFSTIAPYTIEEAYEVADAIARGDLNDLRD
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHH
ELGDLLLQVVYHAQMAEEAGEFAFPDVVQAITTKMIRRHPHVFGDEEARGAGMAKGMWEK
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHH
IKAEEKAEKRQARLARGLDPEDNGKGFLDSVPVALPALTRALKLQEKAARVGFDWSEAAP
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ILDKIEEEIGELREALARGETASIKDEFGDMLFAVVNLGRHLKLDSEAALSGTNEKFRSR
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHH
FHYVEQALEKSGNTLEKATLDEMEALWQEAKSAK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]