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The map label for this gene is ald [H]

Identifier: 13470604

GI number: 13470604

Start: 255890

End: 257005

Strand: Reverse

Name: ald [H]

Synonym: mll0362

Alternate gene names: 13470604

Gene position: 257005-255890 (Counterclockwise)

Preceding gene: 13470606

Following gene: 13470603

Centisome position: 3.65

GC content: 67.38

Gene sequence:

>1116_bases
ATGCGCGTCGGCTGCCCCAAGGAAATCAAGAACCACGAATATCGCGTCGGCCTCACGCCAGGCTCGGTCCGCGAATATGT
GGCGCACGGCCACGAAGTGCTGGTCGAGGCCGGCGCAGGCGCCGGCATCGGCGCCGATGACAACGCCTATCGCGCCGCCG
GCGCCACCATCGCCAAGACCGCCGCCGACGTGTTCGCCAAGTCGGACATGATCGTCAAGGTCAAGGAGCCGCAGCCCAAT
GAGTGGGTTCAACTGCGCGACGGCCAGATCCTGTACACCTACCTTCATCTGGCTCCGGATCCGGAACAGACCAAGGGCCT
ACTCGCTTCGGGCGTCACGGCGATCGCCTACGAAACCGTCACCGACGATCGCGGCGGCCTGCCGCTGCTCGCACCGATGT
CGGAAGTCGCCGGCCGGCTGTCGATCCAGGCCGGCGCCACGGCGCTGCAGAAGGCCAATGGCGGCCGCGGCGTGCTGCTC
GGCGGCGTGCCCGGCGTGCTGCCCGGCAAGGTCACCGTGCTCGGCGGCGGCGTCGTCGGCCTGCACGCGGCCCGCATGGC
CGCCGGCCTTGGCGCCGACGTCACCATCATCGACCGCTCGATCCCGCGACTGCGCCAACTCGACGACCTCTTTGCCGGCC
GTGTGCACACCCGCTACTCCACCGTCGAAGCGCTCGAGGAAGAATGCTTCTCGGCCGACATCGTCGTCGGCGCTGTGCTG
ATCCCTGGCGCTGCAGCGCCCAAGCTCGTCACCCGCGAAATGCTGTCGGGCATGAAGAAGGGCTCGGTGCTGGTCGACGT
CGCCATCGACCAGGGCGGCTGCTTCGAGACCTCGCATGCGACGACTCACGCCGAGCCGACCTACGAAGTCGATGGCGTCA
TCCACTATTGCGTCGCTAACATGCCAGGTGCCGTGCCGGTCACCTCGGCCCACGCCCTTAACAACGCCACGCTGCACTAC
GGCCTGCAGCTCGCCGACAAGGGCCTCAAGGCGCTGGTCGACGACCATCATTTGCGCAACGGCCTCAATGTCCACAAGGG
CAAGATCACCAACCGCGCGGTCGCCGAAGCGCTCGGCTACGAGCTGGTCGAGCCGAAAGCAGTTCTCGCGGCCTGA

Upstream 100 bases:

>100_bases
TGCACACGCAAGCGCATTCGCAAACACTTGCGAGCCGATTCATGGTTCACTCCGACAATACAGGGAGTGAGCCCAAAAGG
GCCCGGATCAGGAGAAGAAA

Downstream 100 bases:

>100_bases
TACACCCAGAAAAAGTCAATTTGCTGTTGTTGCCCGCCGGTCCACTCCGGCGGGCTTTTTATTGGCCTTTCTAGGCGACA
AGCCTGGTTACTCGGCAGAT

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRVGCPKEIKNHEYRVGLTPGSVREYVAHGHEVLVEAGAGAGIGADDNAYRAAGATIAKTAADVFAKSDMIVKVKEPQPN
EWVQLRDGQILYTYLHLAPDPEQTKGLLASGVTAIAYETVTDDRGGLPLLAPMSEVAGRLSIQAGATALQKANGGRGVLL
GGVPGVLPGKVTVLGGGVVGLHAARMAAGLGADVTIIDRSIPRLRQLDDLFAGRVHTRYSTVEALEEECFSADIVVGAVL
IPGAAAPKLVTREMLSGMKKGSVLVDVAIDQGGCFETSHATTHAEPTYEVDGVIHYCVANMPGAVPVTSAHALNNATLHY
GLQLADKGLKALVDDHHLRNGLNVHKGKITNRAVAEALGYELVEPKAVLAA

Sequences:

>Translated_371_residues
MRVGCPKEIKNHEYRVGLTPGSVREYVAHGHEVLVEAGAGAGIGADDNAYRAAGATIAKTAADVFAKSDMIVKVKEPQPN
EWVQLRDGQILYTYLHLAPDPEQTKGLLASGVTAIAYETVTDDRGGLPLLAPMSEVAGRLSIQAGATALQKANGGRGVLL
GGVPGVLPGKVTVLGGGVVGLHAARMAAGLGADVTIIDRSIPRLRQLDDLFAGRVHTRYSTVEALEEECFSADIVVGAVL
IPGAAAPKLVTREMLSGMKKGSVLVDVAIDQGGCFETSHATTHAEPTYEVDGVIHYCVANMPGAVPVTSAHALNNATLHY
GLQLADKGLKALVDDHHLRNGLNVHKGKITNRAVAEALGYELVEPKAVLAA
>Mature_371_residues
MRVGCPKEIKNHEYRVGLTPGSVREYVAHGHEVLVEAGAGAGIGADDNAYRAAGATIAKTAADVFAKSDMIVKVKEPQPN
EWVQLRDGQILYTYLHLAPDPEQTKGLLASGVTAIAYETVTDDRGGLPLLAPMSEVAGRLSIQAGATALQKANGGRGVLL
GGVPGVLPGKVTVLGGGVVGLHAARMAAGLGADVTIIDRSIPRLRQLDDLFAGRVHTRYSTVEALEEECFSADIVVGAVL
IPGAAAPKLVTREMLSGMKKGSVLVDVAIDQGGCFETSHATTHAEPTYEVDGVIHYCVANMPGAVPVTSAHALNNATLHY
GLQLADKGLKALVDDHHLRNGLNVHKGKITNRAVAEALGYELVEPKAVLAA

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=343, Percent_Identity=30.9037900874636, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI122939153, Length=343, Percent_Identity=30.9037900874636, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1787887, Length=377, Percent_Identity=32.0954907161804, Blast_Score=135, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17550456, Length=376, Percent_Identity=30.0531914893617, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 38726; Mature: 38726

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGCPKEIKNHEYRVGLTPGSVREYVAHGHEVLVEAGAGAGIGADDNAYRAAGATIAKT
CCCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHCCCHHHHH
AADVFAKSDMIVKVKEPQPNEWVQLRDGQILYTYLHLAPDPEQTKGLLASGVTAIAYETV
HHHHHHCCCEEEEECCCCCCCCEEEECCEEEEEEEEECCCHHHHHHHHHCCCEEEEEEEE
TDDRGGLPLLAPMSEVAGRLSIQAGATALQKANGGRGVLLGGVPGVLPGKVTVLGGGVVG
CCCCCCCEEECCHHHHHHEEEEHHCHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCHHH
LHAARMAAGLGADVTIIDRSIPRLRQLDDLFAGRVHTRYSTVEALEEECFSADIVVGAVL
HHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHEEEEEE
IPGAAAPKLVTREMLSGMKKGSVLVDVAIDQGGCFETSHATTHAEPTYEVDGVIHYCVAN
CCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCEEHHHHHHHHHHC
MPGAVPVTSAHALNNATLHYGLQLADKGLKALVDDHHLRNGLNVHKGKITNRAVAEALGY
CCCCCCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
ELVEPKAVLAA
HHCCCHHHHCC
>Mature Secondary Structure
MRVGCPKEIKNHEYRVGLTPGSVREYVAHGHEVLVEAGAGAGIGADDNAYRAAGATIAKT
CCCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHCCCHHHHH
AADVFAKSDMIVKVKEPQPNEWVQLRDGQILYTYLHLAPDPEQTKGLLASGVTAIAYETV
HHHHHHCCCEEEEECCCCCCCCEEEECCEEEEEEEEECCCHHHHHHHHHCCCEEEEEEEE
TDDRGGLPLLAPMSEVAGRLSIQAGATALQKANGGRGVLLGGVPGVLPGKVTVLGGGVVG
CCCCCCCEEECCHHHHHHEEEEHHCHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCHHH
LHAARMAAGLGADVTIIDRSIPRLRQLDDLFAGRVHTRYSTVEALEEECFSADIVVGAVL
HHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHEEEEEE
IPGAAAPKLVTREMLSGMKKGSVLVDVAIDQGGCFETSHATTHAEPTYEVDGVIHYCVAN
CCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCEEHHHHHHHHHHC
MPGAVPVTSAHALNNATLHYGLQLADKGLKALVDDHHLRNGLNVHKGKITNRAVAEALGY
CCCCCCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
ELVEPKAVLAA
HHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]