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The map label for this gene is glxR [H]

Identifier: 13470524

GI number: 13470524

Start: 180247

End: 181164

Strand: Direct

Name: glxR [H]

Synonym: mlr0254

Alternate gene names: 13470524

Gene position: 180247-181164 (Clockwise)

Preceding gene: 13470523

Following gene: 13470528

Centisome position: 2.56

GC content: 64.05

Gene sequence:

>918_bases
ATGGAAACCATAGGCTTCATCGGTCTCGGCATCATGGGCGCGCCGATGGCGGGGCATCTGCTCGATGCCGGCTATAAGGT
CATCGCCAGCGACCATCGCTCGAAGCCGCTGGCTGATCTCGTAGCCAAGGGCCTGAAAACAGTCTCCGGCCACGCCGCCG
TCGCCAAGGCGGCCGACATCATCATCACCATGGTGCCGGATACCCCGCAGGTCGCCGATGTGCTGTTCGGCGACAATGGC
GTCGCGTCCGGCCTGACCAGGGGCAAGCTGGTCATCGACATGAGCTCGATCTCGCCGATCGAGACCAAGGTCTTCGCCGG
CAAGATCAACGAACTCGGCTGCGACTATCTCGACGCGCCGGTCTCGGGCGGCGAGGTCGGCGCCAAAGCGGCGTCGCTGA
CCATCATGGTCGGCGGCGAGGAAAAGGCCTTCGAGCGCGCCAAACCCGTCTTCGAGAAGATGGGCAAGAACATCACTTTG
GTCGGCCCCAACGGCGTCGGCCAGACCACTAAGGTCGCGAACCAGATCGTCGTCGCGTTGACCATTGAAGCCGTCGCCGA
AGCGCTTGTTTTTGCCTCGAAAGCCGGCGCCGACCCAGCCAGGGTCAGGCAGGCGCTGATGGGTGGGCTGGCGGCGTCAC
GCATCCTCGAAGTGCATGGCGAGCGCATGGTCAAGCGCACCTTCGCGCCGGGCTTCCGCATCGAACTGCACCAGAAGGAT
CTCAACCTGGCATTGGAGGGCGCCAAGGCGCTCGGCGTCTCGTTGCCCAACACCTCGACCACCCAGCAGCTGTTCAACTC
GTGTGCGGCCAATGGCGGCGCCAGGGAGGATCACTCGGCTCTGGTCAGGGCTCTGGAGCGGATGGCGAATTTCGAGGTCG
GTGGCGCAGTGGCCGAAGTGAAAGGCAAAGCGGCATAG

Upstream 100 bases:

>100_bases
CGGCGCCGAAGCAGCCAATTTCCCCCCTCGTGGGGGAGATGTCCGGCAGGACAGAGGGGGGCGTGACAGAACGCGGCCTA
ACAAAGGACGGAGAAAAACA

Downstream 100 bases:

>100_bases
GCCGGGAGAGGGCGGCGCAACGTTCCCAAGCCGCCACCTCTTGTCCTGCACAAGAACGGGTTCCCGCGCTGTCGCCGGAA
CCCGTTTCTCGTCTTTTTGT

Product: oxidoreductase

Products: NA

Alternate protein names: Tartronate semialdehyde reductase; TSAR [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
METIGFIGLGIMGAPMAGHLLDAGYKVIASDHRSKPLADLVAKGLKTVSGHAAVAKAADIIITMVPDTPQVADVLFGDNG
VASGLTRGKLVIDMSSISPIETKVFAGKINELGCDYLDAPVSGGEVGAKAASLTIMVGGEEKAFERAKPVFEKMGKNITL
VGPNGVGQTTKVANQIVVALTIEAVAEALVFASKAGADPARVRQALMGGLAASRILEVHGERMVKRTFAPGFRIELHQKD
LNLALEGAKALGVSLPNTSTTQQLFNSCAANGGAREDHSALVRALERMANFEVGGAVAEVKGKAA

Sequences:

>Translated_305_residues
METIGFIGLGIMGAPMAGHLLDAGYKVIASDHRSKPLADLVAKGLKTVSGHAAVAKAADIIITMVPDTPQVADVLFGDNG
VASGLTRGKLVIDMSSISPIETKVFAGKINELGCDYLDAPVSGGEVGAKAASLTIMVGGEEKAFERAKPVFEKMGKNITL
VGPNGVGQTTKVANQIVVALTIEAVAEALVFASKAGADPARVRQALMGGLAASRILEVHGERMVKRTFAPGFRIELHQKD
LNLALEGAKALGVSLPNTSTTQQLFNSCAANGGAREDHSALVRALERMANFEVGGAVAEVKGKAA
>Mature_305_residues
METIGFIGLGIMGAPMAGHLLDAGYKVIASDHRSKPLADLVAKGLKTVSGHAAVAKAADIIITMVPDTPQVADVLFGDNG
VASGLTRGKLVIDMSSISPIETKVFAGKINELGCDYLDAPVSGGEVGAKAASLTIMVGGEEKAFERAKPVFEKMGKNITL
VGPNGVGQTTKVANQIVVALTIEAVAEALVFASKAGADPARVRQALMGGLAASRILEVHGERMVKRTFAPGFRIELHQKD
LNLALEGAKALGVSLPNTSTTQQLFNSCAANGGAREDHSALVRALERMANFEVGGAVAEVKGKAA

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=297, Percent_Identity=31.986531986532, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI40556376, Length=284, Percent_Identity=26.7605633802817, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1786719, Length=293, Percent_Identity=66.5529010238908, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI145693186, Length=290, Percent_Identity=42.7586206896552, Blast_Score=244, Evalue=4e-66,
Organism=Escherichia coli, GI1790315, Length=285, Percent_Identity=32.9824561403509, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1789092, Length=282, Percent_Identity=32.9787234042553, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557316, Length=277, Percent_Identity=33.2129963898917, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24655230, Length=300, Percent_Identity=37, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI19922568, Length=300, Percent_Identity=37, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=27.4021352313167, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- InterPro:   IPR006398 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.60 [H]

Molecular weight: Translated: 31465; Mature: 31465

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIGFIGLGIMGAPMAGHLLDAGYKVIASDHRSKPLADLVAKGLKTVSGHAAVAKAADI
CCCCCEEEHHHHCCCHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHCEE
IITMVPDTPQVADVLFGDNGVASGLTRGKLVIDMSSISPIETKVFAGKINELGCDYLDAP
EEEECCCCCHHHHHEECCCCHHCCCCCCEEEEEECCCCCCHHHHHHCCHHHCCCCCCCCC
VSGGEVGAKAASLTIMVGGEEKAFERAKPVFEKMGKNITLVGPNGVGQTTKVANQIVVAL
CCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHEEHEE
TIEAVAEALVFASKAGADPARVRQALMGGLAASRILEVHGERMVKRTFAPGFRIELHQKD
EHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
LNLALEGAKALGVSLPNTSTTQQLFNSCAANGGAREDHSALVRALERMANFEVGGAVAEV
CCEEEECCHHEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEC
KGKAA
CCCCC
>Mature Secondary Structure
METIGFIGLGIMGAPMAGHLLDAGYKVIASDHRSKPLADLVAKGLKTVSGHAAVAKAADI
CCCCCEEEHHHHCCCHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHCEE
IITMVPDTPQVADVLFGDNGVASGLTRGKLVIDMSSISPIETKVFAGKINELGCDYLDAP
EEEECCCCCHHHHHEECCCCHHCCCCCCEEEEEECCCCCCHHHHHHCCHHHCCCCCCCCC
VSGGEVGAKAASLTIMVGGEEKAFERAKPVFEKMGKNITLVGPNGVGQTTKVANQIVVAL
CCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHEEHEE
TIEAVAEALVFASKAGADPARVRQALMGGLAASRILEVHGERMVKRTFAPGFRIELHQKD
EHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
LNLALEGAKALGVSLPNTSTTQQLFNSCAANGGAREDHSALVRALERMANFEVGGAVAEV
CCEEEECCHHEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEC
KGKAA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10601204; 9278503 [H]