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The map label for this gene is potD [H]

Identifier: 13470509

GI number: 13470509

Start: 166605

End: 167696

Strand: Reverse

Name: potD [H]

Synonym: mll0236

Alternate gene names: 13470509

Gene position: 167696-166605 (Counterclockwise)

Preceding gene: 13470511

Following gene: 13470508

Centisome position: 2.38

GC content: 63.1

Gene sequence:

>1092_bases
ATGTCCGTCAAATCCGCATCCGGGAATCCGCTTTCCATCCTGAAGCGCCATCTGCGCGCCGCCTGCGCCGCCACGGCACT
GCTGCTTGGCGTGGGCAGCCTCGCCGAGGCCGCCGATCTCAACGCGCTGATCTGGTGCGACCATTCCGATCCGGCCCTGT
TGCAGCCCTTCGAGGAAGCCAACAACGTCAAGGTCAACGTCAAGGAGTTCGAAGGCACCGGTGCTGGCCTGGCCATCGTC
GAACAGTCGCAGCCCGGCGACTGGGACGTGATGGTGATCGATAGCATCGACGTGCCGCGCGGCGTCGAAAAGGGCCTGTT
CGAGCCACTGCCCGAAGACAAGCTGCCCCTCGCCGATCTCTTCCCGCAAGTGAAGATGGACGGCTCGACCGTGGTCGGCG
GCAAGCGCTACGGCATCACCGAAAAATTCGGCTACAACACGATCGGCTTCAACAAGACCAAGGTCGACCCGGCCGACATG
CAGTCCCTGGCGTCGCTGACCAGCGATAAATACAAGGGCAAGGTCGCCATCTACGACTATTACCTGCCGGTCATCGGCAT
GGCGGCACTCGCCATCGGCAAGAAGACGGCCGATCTGACCGAAGCCGACCTGCCCGCCCTCAAGGCCGAGCTGCTCAAGA
TGAAGGCCAACGCTAAGCTGGTCGGCGAAGTCACCGCCAGCCAGACCGCGCTTGCTACCGGCGAGGTCGACATATTGGTC
GGCGGCGGCGAATGGGTGACGGCGGGGCTGGCCAAGGAGAACCCGGCGCTCGACTTCTCCATCCCCAAGGAGGGCGCGGT
GCTGTGGTCGCAGTCGCTGGCCATGTTCAAGGCGTCCAAGAACAAGGATCTGGCGCTGAAATTCATCCAGTACATTATGA
GCCCCGAGGGCCAGGCGCGTCTTGCCACCTCGTCCTGCTATTGGGGCATGCCCGCCAACAGCAAGGCGGCGCTGACCGAC
GACCAGAAAAAGGTCCTGCGCTTCGACGAGCAGCCGGGCTTCCTCGCCCGTGCGCAAGCCTATCCCGCGCCGAACGCCGA
TCTCGACAAGAAGATGCAGGATATGTGGACCGAAATGCTGCAGGCGAAGTGA

Upstream 100 bases:

>100_bases
TCCGCTCATAGCAGCCTCTTGCCACAAAAACAGAATTGACTCAATTTTAGAATTGGTTCAATTTTTTGCAAGAAGCCACA
AAGGCAAGGGAGAACACTCG

Downstream 100 bases:

>100_bases
GCTGATACCGCTCGTGAGTTCAAACGGTAACAGCGGAAATGCCCTGCCCCGGGCGGGCCGCGCCTTGCCCTGGGCGCTGG
TGACACCGGCGCTCGGCTGG

Product: binding protein component of ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSVKSASGNPLSILKRHLRAACAATALLLGVGSLAEAADLNALIWCDHSDPALLQPFEEANNVKVNVKEFEGTGAGLAIV
EQSQPGDWDVMVIDSIDVPRGVEKGLFEPLPEDKLPLADLFPQVKMDGSTVVGGKRYGITEKFGYNTIGFNKTKVDPADM
QSLASLTSDKYKGKVAIYDYYLPVIGMAALAIGKKTADLTEADLPALKAELLKMKANAKLVGEVTASQTALATGEVDILV
GGGEWVTAGLAKENPALDFSIPKEGAVLWSQSLAMFKASKNKDLALKFIQYIMSPEGQARLATSSCYWGMPANSKAALTD
DQKKVLRFDEQPGFLARAQAYPAPNADLDKKMQDMWTEMLQAK

Sequences:

>Translated_363_residues
MSVKSASGNPLSILKRHLRAACAATALLLGVGSLAEAADLNALIWCDHSDPALLQPFEEANNVKVNVKEFEGTGAGLAIV
EQSQPGDWDVMVIDSIDVPRGVEKGLFEPLPEDKLPLADLFPQVKMDGSTVVGGKRYGITEKFGYNTIGFNKTKVDPADM
QSLASLTSDKYKGKVAIYDYYLPVIGMAALAIGKKTADLTEADLPALKAELLKMKANAKLVGEVTASQTALATGEVDILV
GGGEWVTAGLAKENPALDFSIPKEGAVLWSQSLAMFKASKNKDLALKFIQYIMSPEGQARLATSSCYWGMPANSKAALTD
DQKKVLRFDEQPGFLARAQAYPAPNADLDKKMQDMWTEMLQAK
>Mature_362_residues
SVKSASGNPLSILKRHLRAACAATALLLGVGSLAEAADLNALIWCDHSDPALLQPFEEANNVKVNVKEFEGTGAGLAIVE
QSQPGDWDVMVIDSIDVPRGVEKGLFEPLPEDKLPLADLFPQVKMDGSTVVGGKRYGITEKFGYNTIGFNKTKVDPADMQ
SLASLTSDKYKGKVAIYDYYLPVIGMAALAIGKKTADLTEADLPALKAELLKMKANAKLVGEVTASQTALATGEVDILVG
GGEWVTAGLAKENPALDFSIPKEGAVLWSQSLAMFKASKNKDLALKFIQYIMSPEGQARLATSSCYWGMPANSKAALTDD
QKKVLRFDEQPGFLARAQAYPAPNADLDKKMQDMWTEMLQAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=281, Percent_Identity=26.6903914590747, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787078, Length=373, Percent_Identity=25.201072386059, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39003; Mature: 38872

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKSASGNPLSILKRHLRAACAATALLLGVGSLAEAADLNALIWCDHSDPALLQPFEEA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCHHHCCHHHC
NNVKVNVKEFEGTGAGLAIVEQSQPGDWDVMVIDSIDVPRGVEKGLFEPLPEDKLPLADL
CCEEEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCHHHH
FPQVKMDGSTVVGGKRYGITEKFGYNTIGFNKTKVDPADMQSLASLTSDKYKGKVAIYDY
CCCCCCCCCEEECCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEH
YLPVIGMAALAIGKKTADLTEADLPALKAELLKMKANAKLVGEVTASQTALATGEVDILV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCEEEEE
GGGEWVTAGLAKENPALDFSIPKEGAVLWSQSLAMFKASKNKDLALKFIQYIMSPEGQAR
ECCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEE
LATSSCYWGMPANSKAALTDDQKKVLRFDEQPGFLARAQAYPAPNADLDKKMQDMWTEML
EEECCEEECCCCCCCCCCCCCHHHHHEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHH
QAK
HCC
>Mature Secondary Structure 
SVKSASGNPLSILKRHLRAACAATALLLGVGSLAEAADLNALIWCDHSDPALLQPFEEA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCHHHCCHHHC
NNVKVNVKEFEGTGAGLAIVEQSQPGDWDVMVIDSIDVPRGVEKGLFEPLPEDKLPLADL
CCEEEEEEEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCHHHH
FPQVKMDGSTVVGGKRYGITEKFGYNTIGFNKTKVDPADMQSLASLTSDKYKGKVAIYDY
CCCCCCCCCEEECCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEH
YLPVIGMAALAIGKKTADLTEADLPALKAELLKMKANAKLVGEVTASQTALATGEVDILV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCEEEEE
GGGEWVTAGLAKENPALDFSIPKEGAVLWSQSLAMFKASKNKDLALKFIQYIMSPEGQAR
ECCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEE
LATSSCYWGMPANSKAALTDDQKKVLRFDEQPGFLARAQAYPAPNADLDKKMQDMWTEML
EEECCEEECCCCCCCCCCCCCHHHHHEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHH
QAK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]