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The map label for this gene is gatA [H]

Identifier: 13470503

GI number: 13470503

Start: 160894

End: 162321

Strand: Direct

Name: gatA [H]

Synonym: mlr0229

Alternate gene names: 13470503

Gene position: 160894-162321 (Clockwise)

Preceding gene: 13470502

Following gene: 13470504

Centisome position: 2.29

GC content: 64.08

Gene sequence:

>1428_bases
ATGTCCGATCTCGACCTTTGCTACATGCCGGCCCATGAGGCGCTGCGGCTGTTCAAGGCGAAGAAACTCTCGCCTGTCGA
ATTGATGCAGGCCACTATTCGCCGCGCCGAAGCCACCAGATCGTCGATCAACTGCTTCACCTTCACGCATTTCGAAGAGG
CGATGAAGCTGGCCGCCAGGGCCGAGGCGAAATACGCCAGGGGCGCCAGGGCCGGCGCGCTCGAAGGCCTGCCGATCGGC
ATCAAGGACGAGAGCTACATCAAGGGCAAGCCGACCTCGCATGGCTCGCTGCTGTCCAGGGATGCAATAGGCGGCCACAC
CTCGACGATGAACGAGCGCATCATGAAGGCCGGCGGCATCGTGCATGCCCGCACGGCGACACCCGAGTTCAGCTGCGCGG
GCTATACCTGGTCGAAGCGGTGGGGCGTCACCCGCAACCCGTGGAATCCAGACTTCACGCCGGGCGGTTCGTCGGGTGGT
GCTGCGGCAACGCTCGCTTCCGGAACATCATCGATCGCCACCGGTTCGGACATTGCCGGATCGATCCGCATTCCCGCCTC
AGCCTGCGGTCTCGTCGGCTACAAGCCGCCTTACGGCCGCAATCCCGATGAGCCGCCCTTCAACCTCGATTTCTACTGCC
ACACCGGGCCGCTGGCGCGAAACGTCAGGGATGCGATCCTGCTGCAGAACGTCATGGCCGGACCGAGCCCACTCGACATC
GCCACGCTGCGTCCCAAATTACGCCTGCCGTTCGACTTCAAGCCGATCAAAGGCTGGAGAATAGCCTTTTCCATGGACCT
CGGCTCCTTCGAGGTGGACCCTGACGTGCGCAAGAACACGCTCGCCGCCTGCGATGTGTTCCGTTCGCTCGGCGCCACGG
TCGAGGAAGTCGATCTTGGCTGGGATGATGGGGTGCTCAAGGCCGGGATGGCCTATCTCGAGCACCTGTTCGGCGCGTCG
CTGTCGCAACTGCTCGCGGAGCACGCCAAAGACATGACCAGCTATGCCCGGCGGTTTGCCGAGGATGGGCGAAAGTCCAA
GGCCACCGACTTTGTGGCGACGCTGGATGTGGCGGCCAGGATGTACCAGACGCTCGGTCCGCTGCTGGAGACCTACGATG
TGCTGATCTGCCCGACAAACGCGCTGCCCGCGGTGCCGGCGGAGTTCGACCAGACCACGGACAGGGTCGAAATCAACGGC
ACAGAGGTGAACCCCTCGCTGGGCTGGGTGATGACGACGCCGTTCAACATGCTGAGCCGTTGCCCGGTTCTGTCCATACC
GTCGGGCCACGCCGCCAGCGGCGTGCCGACAGGCATCCAGATCGTCGGCCGCACCTATAGCGACGCCGATGTCTTTCGGG
CCGGGCTGGCCTATGAAACGGCCCGGGGGCAATGGTATGGGGACACCGGGACACGCCCGTCGCTCTGA

Upstream 100 bases:

>100_bases
TAGCGTCGCCGGACCACCCAACCAATAGCCACCGGACCGCGACGCGCGGTCCGGCAACCCTTCATGCCAGGTGCGGTCTC
GCGATCGCGGGAGATTTCCG

Downstream 100 bases:

>100_bases
GTCATTCCGGGATCAGCCGGCGTGGCATATGTAGCAAGGCAATCTGTGCGGCGGGATATGTCCGCCGCGGGTCCGAAAGG
CCTGACGAACATGGCGCAAC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSDLDLCYMPAHEALRLFKAKKLSPVELMQATIRRAEATRSSINCFTFTHFEEAMKLAARAEAKYARGARAGALEGLPIG
IKDESYIKGKPTSHGSLLSRDAIGGHTSTMNERIMKAGGIVHARTATPEFSCAGYTWSKRWGVTRNPWNPDFTPGGSSGG
AAATLASGTSSIATGSDIAGSIRIPASACGLVGYKPPYGRNPDEPPFNLDFYCHTGPLARNVRDAILLQNVMAGPSPLDI
ATLRPKLRLPFDFKPIKGWRIAFSMDLGSFEVDPDVRKNTLAACDVFRSLGATVEEVDLGWDDGVLKAGMAYLEHLFGAS
LSQLLAEHAKDMTSYARRFAEDGRKSKATDFVATLDVAARMYQTLGPLLETYDVLICPTNALPAVPAEFDQTTDRVEING
TEVNPSLGWVMTTPFNMLSRCPVLSIPSGHAASGVPTGIQIVGRTYSDADVFRAGLAYETARGQWYGDTGTRPSL

Sequences:

>Translated_475_residues
MSDLDLCYMPAHEALRLFKAKKLSPVELMQATIRRAEATRSSINCFTFTHFEEAMKLAARAEAKYARGARAGALEGLPIG
IKDESYIKGKPTSHGSLLSRDAIGGHTSTMNERIMKAGGIVHARTATPEFSCAGYTWSKRWGVTRNPWNPDFTPGGSSGG
AAATLASGTSSIATGSDIAGSIRIPASACGLVGYKPPYGRNPDEPPFNLDFYCHTGPLARNVRDAILLQNVMAGPSPLDI
ATLRPKLRLPFDFKPIKGWRIAFSMDLGSFEVDPDVRKNTLAACDVFRSLGATVEEVDLGWDDGVLKAGMAYLEHLFGAS
LSQLLAEHAKDMTSYARRFAEDGRKSKATDFVATLDVAARMYQTLGPLLETYDVLICPTNALPAVPAEFDQTTDRVEING
TEVNPSLGWVMTTPFNMLSRCPVLSIPSGHAASGVPTGIQIVGRTYSDADVFRAGLAYETARGQWYGDTGTRPSL
>Mature_474_residues
SDLDLCYMPAHEALRLFKAKKLSPVELMQATIRRAEATRSSINCFTFTHFEEAMKLAARAEAKYARGARAGALEGLPIGI
KDESYIKGKPTSHGSLLSRDAIGGHTSTMNERIMKAGGIVHARTATPEFSCAGYTWSKRWGVTRNPWNPDFTPGGSSGGA
AATLASGTSSIATGSDIAGSIRIPASACGLVGYKPPYGRNPDEPPFNLDFYCHTGPLARNVRDAILLQNVMAGPSPLDIA
TLRPKLRLPFDFKPIKGWRIAFSMDLGSFEVDPDVRKNTLAACDVFRSLGATVEEVDLGWDDGVLKAGMAYLEHLFGASL
SQLLAEHAKDMTSYARRFAEDGRKSKATDFVATLDVAARMYQTLGPLLETYDVLICPTNALPAVPAEFDQTTDRVEINGT
EVNPSLGWVMTTPFNMLSRCPVLSIPSGHAASGVPTGIQIVGRTYSDADVFRAGLAYETARGQWYGDTGTRPSL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=27.7665995975855, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI222831590, Length=512, Percent_Identity=25.9765625, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=352, Percent_Identity=28.4090909090909, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=226, Percent_Identity=28.7610619469027, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=261, Percent_Identity=29.5019157088123, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=183, Percent_Identity=33.879781420765, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=183, Percent_Identity=33.879781420765, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=288, Percent_Identity=26.7361111111111, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=177, Percent_Identity=32.7683615819209, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320448, Length=187, Percent_Identity=35.2941176470588, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319685, Length=386, Percent_Identity=26.1658031088083, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550774, Length=255, Percent_Identity=32.5490196078431, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24648435, Length=255, Percent_Identity=32.5490196078431, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24648437, Length=255, Percent_Identity=32.5490196078431, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24644968, Length=488, Percent_Identity=25.2049180327869, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078093, Length=508, Percent_Identity=25.5905511811024, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652985, Length=488, Percent_Identity=25, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922090, Length=488, Percent_Identity=25, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652981, Length=488, Percent_Identity=25, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24652983, Length=488, Percent_Identity=25, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648441, Length=180, Percent_Identity=36.1111111111111, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648439, Length=180, Percent_Identity=36.1111111111111, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648113, Length=483, Percent_Identity=25.879917184265, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356731, Length=270, Percent_Identity=28.1481481481481, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 51241; Mature: 51110

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLDLCYMPAHEALRLFKAKKLSPVELMQATIRRAEATRSSINCFTFTHFEEAMKLAAR
CCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
AEAKYARGARAGALEGLPIGIKDESYIKGKPTSHGSLLSRDAIGGHTSTMNERIMKAGGI
HHHHHHCCCCCCCCCCCEECCCCCCEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCE
VHARTATPEFSCAGYTWSKRWGVTRNPWNPDFTPGGSSGGAAATLASGTSSIATGSDIAG
EEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
SIRIPASACGLVGYKPPYGRNPDEPPFNLDFYCHTGPLARNVRDAILLQNVMAGPSPLDI
EEECCHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEE
ATLRPKLRLPFDFKPIKGWRIAFSMDLGSFEVDPDVRKNTLAACDVFRSLGATVEEVDLG
EECCCCEECCCCCCCCCCEEEEEEECCCCEECCCCHHHHHHHHHHHHHHHCCCCEECCCC
WDDGVLKAGMAYLEHLFGASLSQLLAEHAKDMTSYARRFAEDGRKSKATDFVATLDVAAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
MYQTLGPLLETYDVLICPTNALPAVPAEFDQTTDRVEINGTEVNPSLGWVMTTPFNMLSR
HHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCEEEECCCEECCCCCEEEECCHHHHHC
CPVLSIPSGHAASGVPTGIQIVGRTYSDADVFRAGLAYETARGQWYGDTGTRPSL
CCEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCEECCCCCEECCCCCCCCC
>Mature Secondary Structure 
SDLDLCYMPAHEALRLFKAKKLSPVELMQATIRRAEATRSSINCFTFTHFEEAMKLAAR
CCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
AEAKYARGARAGALEGLPIGIKDESYIKGKPTSHGSLLSRDAIGGHTSTMNERIMKAGGI
HHHHHHCCCCCCCCCCCEECCCCCCEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCE
VHARTATPEFSCAGYTWSKRWGVTRNPWNPDFTPGGSSGGAAATLASGTSSIATGSDIAG
EEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
SIRIPASACGLVGYKPPYGRNPDEPPFNLDFYCHTGPLARNVRDAILLQNVMAGPSPLDI
EEECCHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEE
ATLRPKLRLPFDFKPIKGWRIAFSMDLGSFEVDPDVRKNTLAACDVFRSLGATVEEVDLG
EECCCCEECCCCCCCCCCEEEEEEECCCCEECCCCHHHHHHHHHHHHHHHCCCCEECCCC
WDDGVLKAGMAYLEHLFGASLSQLLAEHAKDMTSYARRFAEDGRKSKATDFVATLDVAAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
MYQTLGPLLETYDVLICPTNALPAVPAEFDQTTDRVEINGTEVNPSLGWVMTTPFNMLSR
HHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCEEEECCCEECCCCCEEEECCHHHHHC
CPVLSIPSGHAASGVPTGIQIVGRTYSDADVFRAGLAYETARGQWYGDTGTRPSL
CCEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCEECCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA