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The map label for this gene is potD-A [H]

Identifier: 13470502

GI number: 13470502

Start: 159762

End: 160796

Strand: Direct

Name: potD-A [H]

Synonym: mlr0227

Alternate gene names: 13470502

Gene position: 159762-160796 (Clockwise)

Preceding gene: 13470501

Following gene: 13470503

Centisome position: 2.27

GC content: 59.42

Gene sequence:

>1035_bases
ATGAACTGGAAACTGACCGCGGCGGCCGCCGGGCTGTCGCTGCTCGCCGCAACCGGCGTTGCCCGCGCCGATGGCGAACT
CAACATCTACAATTGGGGCAACTACACCAGCCCGGACCTCATCAAGAAGTTCGAGGACACCTACAAGGTCAAGGTCACCG
TCACCGACTATGATTCCAATGACACCGCATTGGCCAAGGTCCGCCAGGGCGCGTCCGGCTATGACATCGTCGTGCCGTCG
GCCAGCGTCGTGCCGGTCTGGATCAGCGAAGGTCTGCTCCTGGAGACCGACCCTAACAAGATGGAGAATTTCAAGAATGT
CGATCCGCGATGGGCCGACGTTCCCTTCGATCCAGGCCGCAAATACACTGTTCCATGGCAGTGGGGCACGACAGGCATTT
CGGTGAACAAGTCGGTGTATTCCGGCGACCCGAACACCTCGGCGATTTTCCTCGACCCGCCGCCGGAGCTTGTCGGCAAG
ATCAATGTCGTGCCCGAAATGGGGGACATCATGTTCCTCGCCATCGCTTATGAAGGCGGTAAATATTGCACCAACGATAT
GCAGGTGCTGAAAAAGGTGCACGACAAGCTGGTCGAGGCGAAGGCGAAATGGCTCTCGCTCGACTACGCGGCCGTAGACG
GCCTGGGCCGGGGCGACATTGCCGCCGGCGTCAACTGGAACGGCATATCGCTGCGCGAGCGGCTGCAGAACAACAAGATC
GTCTACGGCTATCCCAAGGAAGGCTTTCCGATCTGGATGGACAATGTCGCCGTCCTCAAGGACGCCAAGAATGTCGAGAA
CGCCAAGCTGTTCCAGAACTTCATCATGGACCCGAAGAACGCGGCGATGATCTCGGCTTTCGCCCGCTACGCCAACGGCA
TCAAGGGCTCCGAGGCTTTCATGCCGGACGACATGAAGACGGCGCCGGAAGTGGACGTTCCGGCTGAATTTGCCGCCAAG
GGCCAGTTCATGCCGGCCTGTCCATCGGATGTGCAGGCGCTCTACACGAAGATCTGGACCGACCTCCAGAAATAG

Upstream 100 bases:

>100_bases
GCCGGCCCACCTTGGGCCGAAGGGCAGAGCCAGGCTTGTTCATCAGCAGGAGACGGACCGAGGCGGCGACGCAGCCGGTC
CCAACAATGGGAGCACTAGC

Downstream 100 bases:

>100_bases
CGTCGCCGGACCACCCAACCAATAGCCACCGGACCGCGACGCGCGGTCCGGCAACCCTTCATGCCAGGTGCGGTCTCGCG
ATCGCGGGAGATTTCCGATG

Product: binding protein component of ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNWKLTAAAAGLSLLAATGVARADGELNIYNWGNYTSPDLIKKFEDTYKVKVTVTDYDSNDTALAKVRQGASGYDIVVPS
ASVVPVWISEGLLLETDPNKMENFKNVDPRWADVPFDPGRKYTVPWQWGTTGISVNKSVYSGDPNTSAIFLDPPPELVGK
INVVPEMGDIMFLAIAYEGGKYCTNDMQVLKKVHDKLVEAKAKWLSLDYAAVDGLGRGDIAAGVNWNGISLRERLQNNKI
VYGYPKEGFPIWMDNVAVLKDAKNVENAKLFQNFIMDPKNAAMISAFARYANGIKGSEAFMPDDMKTAPEVDVPAEFAAK
GQFMPACPSDVQALYTKIWTDLQK

Sequences:

>Translated_344_residues
MNWKLTAAAAGLSLLAATGVARADGELNIYNWGNYTSPDLIKKFEDTYKVKVTVTDYDSNDTALAKVRQGASGYDIVVPS
ASVVPVWISEGLLLETDPNKMENFKNVDPRWADVPFDPGRKYTVPWQWGTTGISVNKSVYSGDPNTSAIFLDPPPELVGK
INVVPEMGDIMFLAIAYEGGKYCTNDMQVLKKVHDKLVEAKAKWLSLDYAAVDGLGRGDIAAGVNWNGISLRERLQNNKI
VYGYPKEGFPIWMDNVAVLKDAKNVENAKLFQNFIMDPKNAAMISAFARYANGIKGSEAFMPDDMKTAPEVDVPAEFAAK
GQFMPACPSDVQALYTKIWTDLQK
>Mature_344_residues
MNWKLTAAAAGLSLLAATGVARADGELNIYNWGNYTSPDLIKKFEDTYKVKVTVTDYDSNDTALAKVRQGASGYDIVVPS
ASVVPVWISEGLLLETDPNKMENFKNVDPRWADVPFDPGRKYTVPWQWGTTGISVNKSVYSGDPNTSAIFLDPPPELVGK
INVVPEMGDIMFLAIAYEGGKYCTNDMQVLKKVHDKLVEAKAKWLSLDYAAVDGLGRGDIAAGVNWNGISLRERLQNNKI
VYGYPKEGFPIWMDNVAVLKDAKNVENAKLFQNFIMDPKNAAMISAFARYANGIKGSEAFMPDDMKTAPEVDVPAEFAAK
GQFMPACPSDVQALYTKIWTDLQK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=272, Percent_Identity=31.25, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1787078, Length=361, Percent_Identity=27.1468144044321, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37855; Mature: 37855

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWKLTAAAAGLSLLAATGVARADGELNIYNWGNYTSPDLIKKFEDTYKVKVTVTDYDSN
CCEEEEHHHHHHHHHHHHCCEECCCCEEEEECCCCCCHHHHHHHCCCEEEEEEEEECCCC
DTALAKVRQGASGYDIVVPSASVVPVWISEGLLLETDPNKMENFKNVDPRWADVPFDPGR
CHHHHHHHCCCCCCEEEECCCCEEEEEECCCEEEECCCHHHHHCCCCCCCCCCCCCCCCC
KYTVPWQWGTTGISVNKSVYSGDPNTSAIFLDPPPELVGKINVVPEMGDIMFLAIAYEGG
EEEEEECCCCCCEEECCEEECCCCCCEEEEECCCHHHHEEEEECCCCCCEEEEEEEECCC
KYCTNDMQVLKKVHDKLVEAKAKWLSLDYAAVDGLGRGDIAAGVNWNGISLRERLQNNKI
CEEHHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCEECCCCCCCHHHHHHHCCCCE
VYGYPKEGFPIWMDNVAVLKDAKNVENAKLFQNFIMDPKNAAMISAFARYANGIKGSEAF
EEECCCCCCCEEECCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
MPDDMKTAPEVDVPAEFAAKGQFMPACPSDVQALYTKIWTDLQK
CCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNWKLTAAAAGLSLLAATGVARADGELNIYNWGNYTSPDLIKKFEDTYKVKVTVTDYDSN
CCEEEEHHHHHHHHHHHHCCEECCCCEEEEECCCCCCHHHHHHHCCCEEEEEEEEECCCC
DTALAKVRQGASGYDIVVPSASVVPVWISEGLLLETDPNKMENFKNVDPRWADVPFDPGR
CHHHHHHHCCCCCCEEEECCCCEEEEEECCCEEEECCCHHHHHCCCCCCCCCCCCCCCCC
KYTVPWQWGTTGISVNKSVYSGDPNTSAIFLDPPPELVGKINVVPEMGDIMFLAIAYEGG
EEEEEECCCCCCEEECCEEECCCCCCEEEEECCCHHHHEEEEECCCCCCEEEEEEEECCC
KYCTNDMQVLKKVHDKLVEAKAKWLSLDYAAVDGLGRGDIAAGVNWNGISLRERLQNNKI
CEEHHHHHHHHHHHHHHHHHHHHHEEEEHHHHCCCCCCCEECCCCCCCHHHHHHHCCCCE
VYGYPKEGFPIWMDNVAVLKDAKNVENAKLFQNFIMDPKNAAMISAFARYANGIKGSEAF
EEECCCCCCCEEECCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
MPDDMKTAPEVDVPAEFAAKGQFMPACPSDVQALYTKIWTDLQK
CCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]