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The map label for this gene is panB

Identifier: 13470477

GI number: 13470477

Start: 134301

End: 135161

Strand: Reverse

Name: panB

Synonym: mll0197

Alternate gene names: 13470477

Gene position: 135161-134301 (Counterclockwise)

Preceding gene: 13470482

Following gene: 13470476

Centisome position: 1.92

GC content: 65.85

Gene sequence:

>861_bases
TTGGCCACGAAACTGCCGACCACCGACATTCGCATCGGCGCGGAGCTGATTCGCCGCCGTAAGGGCGGCGTCCCGTTGGT
CTGCCTGACCGCCTACACCTATCCCGTCGCCCGCCTGCTCGACCCGCATGTCGACCTGCTTCTGGTCGGCGACAGCGTCT
CCATGGTCCTGCATGGCCATGCGACGACGCTCGGCGCCTCGCTGGGGATGATGATCGCGCATGGGCAAGCGGTGATGCGC
GGCTCGGCCAGGGCCTGCGTGGTCGTCGATATGCCGGCCGGCAGCTACGAGGATTCGGCGGCGCAGGCTGTGGCCTCAGC
GCGGCGCATCGTTGACGAGACCGGCTGCCAGGCGGTGAAGCTTGAAGGCGGCGTCGACATGGCCGGGCAGATTGCGGCAA
TCGCCGCCGCCGGCATTCCGGTCATGGGTCATATCGGCCTGCTGCCGCAATCGGTGGAAAAGGACGGCGGCTACAAGATC
AAGGGCCGCACGGATGAGACTGTCGCGGCGCTGATGGCGGACGCGCGCGCCGTCGAAAAGGCCGGCGCGTTCTCCGTGGT
CATCGAGGGCACCATCGAGGCGGTCGCCGCCGACATCACACGCCGCATCGCCATTCCAACCATCGGCATCGGTGCCAGCG
GCGACTGCGATGGCCAGATTCTGGTCATCGACGACATGGTCGGGCTAACCGTCGATCGCGTGCCGAAATTCGTCAAGGAA
TATGCCGACCTGCGCAGCGTGATCGCGAACGCCGCCGAGCGCTACGCAGCGGAGGTGCGAGACCGGACTTTCCCCGGCCC
TAGCCATGTCTTTTCGGGTTCAAAAGCCTCTTCGGACTTAAACGGCGGGGATGAATCATGA

Upstream 100 bases:

>100_bases
AGGCGACAGGATGTGCGCTTGCCAAGCCTTTGCGCTCTTTCTAAAGCTCAGCGCGCCCATGCGGATATCCAATCCAGCCA
TGGGCCGAGGGAGGTGACGA

Downstream 100 bases:

>100_bases
GCCGTCCCGAGGTCGTCAACAGCGTCGCTGCACTTCGCGCGCAGGTCAGTGACTGGCGGCGCGACGGCCTGCGCGTTGCC
ATGGTGCCAACCATGGGCGC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MATKLPTTDIRIGAELIRRRKGGVPLVCLTAYTYPVARLLDPHVDLLLVGDSVSMVLHGHATTLGASLGMMIAHGQAVMR
GSARACVVVDMPAGSYEDSAAQAVASARRIVDETGCQAVKLEGGVDMAGQIAAIAAAGIPVMGHIGLLPQSVEKDGGYKI
KGRTDETVAALMADARAVEKAGAFSVVIEGTIEAVAADITRRIAIPTIGIGASGDCDGQILVIDDMVGLTVDRVPKFVKE
YADLRSVIANAAERYAAEVRDRTFPGPSHVFSGSKASSDLNGGDES

Sequences:

>Translated_286_residues
MATKLPTTDIRIGAELIRRRKGGVPLVCLTAYTYPVARLLDPHVDLLLVGDSVSMVLHGHATTLGASLGMMIAHGQAVMR
GSARACVVVDMPAGSYEDSAAQAVASARRIVDETGCQAVKLEGGVDMAGQIAAIAAAGIPVMGHIGLLPQSVEKDGGYKI
KGRTDETVAALMADARAVEKAGAFSVVIEGTIEAVAADITRRIAIPTIGIGASGDCDGQILVIDDMVGLTVDRVPKFVKE
YADLRSVIANAAERYAAEVRDRTFPGPSHVFSGSKASSDLNGGDES
>Mature_285_residues
ATKLPTTDIRIGAELIRRRKGGVPLVCLTAYTYPVARLLDPHVDLLLVGDSVSMVLHGHATTLGASLGMMIAHGQAVMRG
SARACVVVDMPAGSYEDSAAQAVASARRIVDETGCQAVKLEGGVDMAGQIAAIAAAGIPVMGHIGLLPQSVEKDGGYKIK
GRTDETVAALMADARAVEKAGAFSVVIEGTIEAVAADITRRIAIPTIGIGASGDCDGQILVIDDMVGLTVDRVPKFVKEY
ADLRSVIANAAERYAAEVRDRTFPGPSHVFSGSKASSDLNGGDES

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=247, Percent_Identity=38.8663967611336, Blast_Score=163, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319653, Length=267, Percent_Identity=37.0786516853933, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_RHILO (Q98NC9)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102046.1
- ProteinModelPortal:   Q98NC9
- SMR:   Q98NC9
- GeneID:   1224709
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll0197
- NMPDR:   fig|266835.1.peg.151
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29638; Mature: 29507

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: ACT_SITE 189-189 BINDING 90-90 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKLPTTDIRIGAELIRRRKGGVPLVCLTAYTYPVARLLDPHVDLLLVGDSVSMVLHGH
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCEEEEECCCEEEEEECC
ATTLGASLGMMIAHGQAVMRGSARACVVVDMPAGSYEDSAAQAVASARRIVDETGCQAVK
HHHHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LEGGVDMAGQIAAIAAAGIPVMGHIGLLPQSVEKDGGYKIKGRTDETVAALMADARAVEK
ECCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
AGAFSVVIEGTIEAVAADITRRIAIPTIGIGASGDCDGQILVIDDMVGLTVDRVPKFVKE
CCCEEEEEEHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
YADLRSVIANAAERYAAEVRDRTFPGPSHVFSGSKASSDLNGGDES
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATKLPTTDIRIGAELIRRRKGGVPLVCLTAYTYPVARLLDPHVDLLLVGDSVSMVLHGH
CCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCEEEEECCCEEEEEECC
ATTLGASLGMMIAHGQAVMRGSARACVVVDMPAGSYEDSAAQAVASARRIVDETGCQAVK
HHHHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LEGGVDMAGQIAAIAAAGIPVMGHIGLLPQSVEKDGGYKIKGRTDETVAALMADARAVEK
ECCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
AGAFSVVIEGTIEAVAADITRRIAIPTIGIGASGDCDGQILVIDDMVGLTVDRVPKFVKE
CCCEEEEEEHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
YADLRSVIANAAERYAAEVRDRTFPGPSHVFSGSKASSDLNGGDES
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968