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The map label for this gene is ttcA [H]

Identifier: 13470364

GI number: 13470364

Start: 37184

End: 37999

Strand: Direct

Name: ttcA [H]

Synonym: mlr0047

Alternate gene names: 13470364

Gene position: 37184-37999 (Clockwise)

Preceding gene: 13470363

Following gene: 13470365

Centisome position: 0.53

GC content: 62.5

Gene sequence:

>816_bases
GTGCCGTCCTCGGTCGAGTTCAACAAATTGCGCAAGCGGCTGCTGCGGCTGACCCGCCAGGCGATCGAGGACTTCTCGAT
GGTGGAGCCGGGCCAGCGCTGGCTGGTCGCGCTGTCGGGCGGCAAGGATTCCTACGGCCTGCTTGCCTTGCTGCTCGACC
TCAAATGGCGCGGGCTGCTGCCGGTCGAATTGCTGGCCTGCAATCTCGACCAGGGCCAGCCGAACTTTCCCAAGCACATC
CTGCCCGATTATCTCAACGCCAACGGCATCGCGCATCGCATCGAATATCAGGACACCTATTCGGTGGTCACTAACAAGCT
GCCGGAAGGCAGCACCTATTGCTCGCTCTGCTCGCGGCTGCGGCGCGGCCATCTCTACCGCATTGCGCGCGAGGAGGGGT
GCTCGGCGCTGGTGCTCGGCCACCACCGCGAGGACATTCTCGAGACCTTCTTCATGAACCTGTTCCATGGCGGCCGTCTC
GCCGCCATGCCGCCAAAGCTGCTCAACGACGAGGGCGACGTCATGGTGCTGCGGCCACTGAGCTATTGCGCCGAGATCGA
CCTCGAGAAATTCGCCGCGGCGATGCGGTTCCCGATCATCCCTTGCGACCTCTGCGGCAGCCAGGAGGGTCTCCAGCGCA
ACGCCATGAAGGCGATGCTGGAAGACATCGAAAAGCGCATGCCCGGCCGCAAGGACACCATGATCCGCGCGCTGTCCAAT
ACCAGGCCGTCGCATCTGCTCGACAGGAAACTGTTCGATTTCGCCGCCCTGAACCAGACCCTCATCACAGGACAAGACGC
TTCAGATGACATTTGA

Upstream 100 bases:

>100_bases
TGCGGACATCACATCCCGGGGCCGCGCCATGAACATGCTGCCAGACATCGAGACGCTTGAAACGGCCGCAGAAGGGGGCT
CCCATCCGCTGTTCGCCGAT

Downstream 100 bases:

>100_bases
CGATAACGCGATCGACTGGCTGGCCAGCCTGCTGGCCGAGGCCGCCGACACCGAAATCATGCCCCGCTTTCGCCGGCTGG
GCGACGGCGATATCAGACAG

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MPSSVEFNKLRKRLLRLTRQAIEDFSMVEPGQRWLVALSGGKDSYGLLALLLDLKWRGLLPVELLACNLDQGQPNFPKHI
LPDYLNANGIAHRIEYQDTYSVVTNKLPEGSTYCSLCSRLRRGHLYRIAREEGCSALVLGHHREDILETFFMNLFHGGRL
AAMPPKLLNDEGDVMVLRPLSYCAEIDLEKFAAAMRFPIIPCDLCGSQEGLQRNAMKAMLEDIEKRMPGRKDTMIRALSN
TRPSHLLDRKLFDFAALNQTLITGQDASDDI

Sequences:

>Translated_271_residues
MPSSVEFNKLRKRLLRLTRQAIEDFSMVEPGQRWLVALSGGKDSYGLLALLLDLKWRGLLPVELLACNLDQGQPNFPKHI
LPDYLNANGIAHRIEYQDTYSVVTNKLPEGSTYCSLCSRLRRGHLYRIAREEGCSALVLGHHREDILETFFMNLFHGGRL
AAMPPKLLNDEGDVMVLRPLSYCAEIDLEKFAAAMRFPIIPCDLCGSQEGLQRNAMKAMLEDIEKRMPGRKDTMIRALSN
TRPSHLLDRKLFDFAALNQTLITGQDASDDI
>Mature_270_residues
PSSVEFNKLRKRLLRLTRQAIEDFSMVEPGQRWLVALSGGKDSYGLLALLLDLKWRGLLPVELLACNLDQGQPNFPKHIL
PDYLNANGIAHRIEYQDTYSVVTNKLPEGSTYCSLCSRLRRGHLYRIAREEGCSALVLGHHREDILETFFMNLFHGGRLA
AMPPKLLNDEGDVMVLRPLSYCAEIDLEKFAAAMRFPIIPCDLCGSQEGLQRNAMKAMLEDIEKRMPGRKDTMIRALSNT
RPSHLLDRKLFDFAALNQTLITGQDASDDI

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=266, Percent_Identity=51.8796992481203, Blast_Score=295, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI193211090, Length=233, Percent_Identity=30.9012875536481, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540090, Length=219, Percent_Identity=26.4840182648402, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 30659; Mature: 30528

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSVEFNKLRKRLLRLTRQAIEDFSMVEPGQRWLVALSGGKDSYGLLALLLDLKWRGLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCC
PVELLACNLDQGQPNFPKHILPDYLNANGIAHRIEYQDTYSVVTNKLPEGSTYCSLCSRL
CHHHHEECCCCCCCCCCHHHCCHHHCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHH
RRGHLYRIAREEGCSALVLGHHREDILETFFMNLFHGGRLAAMPPKLLNDEGDVMVLRPL
HHCHHEEHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCEEEECCH
SYCAEIDLEKFAAAMRFPIIPCDLCGSQEGLQRNAMKAMLEDIEKRMPGRKDTMIRALSN
HHHHHCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
TRPSHLLDRKLFDFAALNQTLITGQDASDDI
CCHHHHHHHHHHHHHHHCHHHCCCCCCCCCC
>Mature Secondary Structure 
PSSVEFNKLRKRLLRLTRQAIEDFSMVEPGQRWLVALSGGKDSYGLLALLLDLKWRGLL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCC
PVELLACNLDQGQPNFPKHILPDYLNANGIAHRIEYQDTYSVVTNKLPEGSTYCSLCSRL
CHHHHEECCCCCCCCCCHHHCCHHHCCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHH
RRGHLYRIAREEGCSALVLGHHREDILETFFMNLFHGGRLAAMPPKLLNDEGDVMVLRPL
HHCHHEEHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCEEEECCH
SYCAEIDLEKFAAAMRFPIIPCDLCGSQEGLQRNAMKAMLEDIEKRMPGRKDTMIRALSN
HHHHHCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
TRPSHLLDRKLFDFAALNQTLITGQDASDDI
CCHHHHHHHHHHHHHHHCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]