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The map label for this gene is pepE

Identifier: 161723315

GI number: 161723315

Start: 528656

End: 529360

Strand: Direct

Name: pepE

Synonym: PM0454

Alternate gene names: 161723315

Gene position: 528656-529360 (Clockwise)

Preceding gene: 15602309

Following gene: 15602320

Centisome position: 23.42

GC content: 40.71

Gene sequence:

>705_bases
ATGAAAAACATGTTATTAATGAGCGGTTCGAAATATCAAAATACAGACTATTTAGTGCATACGCTACCTTGGCTACAGGA
TTTTTTGGCAGACTATCAAGGGAAAACCGTTGCTTTTGTACCTTATGCTGGTGTTCGTCAAAGTTATGATGAGTACGAAT
TAAAAGTCCAAAAAGCATTAGCTGAATTAAATGTGGCGATTCTTTCGGTTCACCGTGCAGAAAAACATGCAGAAATCATT
GAAAAAGCGGATGTTATTGCGATTGGGGGAGGTAATACGTTTTGTTTATTAAAAGGCATGTATGAACATCATTTATTGCC
ATTGATTCGCGAAAAAGTTCAGTCGGGTACACCGTATTTTGGTTGGAGCGCGGGGGCAAATGTGGCAGGTCGCTCTATTA
TGACCACCAATGATATGCCGATTACTTATCCGCCATCTTTTGACGCTTTAAATTTATTTCCACACCAACTAAATCCGCAT
TTTATTTCGGGTAAACCTGCTGGGCATAATGGGGAAAGTCGCGAAGAACGCTTAGCGGAATTCTTAATTGTCAACCCAAC
TGCCAACGTCTATGCCTTGCCAGAAGGAACCGCACTGCATATTCAAGGTCAGCAAGCGCGTGTATTGGGTCAACATGATG
TGTTATTGTTCAGTGAAAATATGCAATTAGCGACCTTGCCGGTGAATAGCGTATTTGATTATTAA

Upstream 100 bases:

>100_bases
ACTGTGATGTGAGTCACGAAATCAACAATGCCGGCTGTTTATTTATGGCGGTGCATTGCATAATGAGAGAAATATGACCA
TGAGTTCAGTGAGGAAAACG

Downstream 100 bases:

>100_bases
TCTCGCTTTCTGGTCTTTTTGTGAACTTTGTTTGGTGAAAAGATCAGAAAGACTTATAATCTGTACTGCTTTTTGATTAA
TAAGAGAGAGAACAAAATGA

Product: peptidase E

Products: NA

Alternate protein names: Alpha-aspartyl dipeptidase; Asp-specific dipeptidase; Dipeptidase E

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKNMLLMSGSKYQNTDYLVHTLPWLQDFLADYQGKTVAFVPYAGVRQSYDEYELKVQKALAELNVAILSVHRAEKHAEII
EKADVIAIGGGNTFCLLKGMYEHHLLPLIREKVQSGTPYFGWSAGANVAGRSIMTTNDMPITYPPSFDALNLFPHQLNPH
FISGKPAGHNGESREERLAEFLIVNPTANVYALPEGTALHIQGQQARVLGQHDVLLFSENMQLATLPVNSVFDY

Sequences:

>Translated_234_residues
MKNMLLMSGSKYQNTDYLVHTLPWLQDFLADYQGKTVAFVPYAGVRQSYDEYELKVQKALAELNVAILSVHRAEKHAEII
EKADVIAIGGGNTFCLLKGMYEHHLLPLIREKVQSGTPYFGWSAGANVAGRSIMTTNDMPITYPPSFDALNLFPHQLNPH
FISGKPAGHNGESREERLAEFLIVNPTANVYALPEGTALHIQGQQARVLGQHDVLLFSENMQLATLPVNSVFDY
>Mature_234_residues
MKNMLLMSGSKYQNTDYLVHTLPWLQDFLADYQGKTVAFVPYAGVRQSYDEYELKVQKALAELNVAILSVHRAEKHAEII
EKADVIAIGGGNTFCLLKGMYEHHLLPLIREKVQSGTPYFGWSAGANVAGRSIMTTNDMPITYPPSFDALNLFPHQLNPH
FISGKPAGHNGESREERLAEFLIVNPTANVYALPEGTALHIQGQQARVLGQHDVLLFSENMQLATLPVNSVFDY

Specific function: Hydrolyzes dipeptides containing N-terminal aspartate residues. May play a role in allowing the cell to use peptide aspartate to spare carbon otherwise required for the synthesis of the aspartate family of amino acids

COG id: COG3340

COG function: function code E; Peptidase E

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S51 family

Homologues:

Organism=Escherichia coli, GI1790452, Length=215, Percent_Identity=43.7209302325581, Blast_Score=182, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24641669, Length=206, Percent_Identity=43.6893203883495, Blast_Score=159, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPE_PASMU (Q9CNH7)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245391.2
- ProteinModelPortal:   Q9CNH7
- SMR:   Q9CNH7
- MEROPS:   S51.001
- GeneID:   1243801
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0454
- NMPDR:   fig|272843.1.peg.454
- HOGENOM:   HBG298965
- OMA:   ALWRQGD
- ProtClustDB:   PRK05282
- BioCyc:   PMUL272843:PM0454-MONOMER
- BRENDA:   3.4.13.21
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00510
- InterPro:   IPR005320
- InterPro:   IPR023172

Pfam domain/function: PF03575 Peptidase_S51

EC number: =3.4.13.21

Molecular weight: Translated: 26058; Mature: 26058

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: ACT_SITE 123-123 ACT_SITE 138-138 ACT_SITE 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNMLLMSGSKYQNTDYLVHTLPWLQDFLADYQGKTVAFVPYAGVRQSYDEYELKVQKAL
CCCEEEECCCCCCCCCEEEEHHHHHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHHH
AELNVAILSVHRAEKHAEIIEKADVIAIGGGNTFCLLKGMYEHHLLPLIREKVQSGTPYF
HHHHHHEEEHHHHHHHHHHHHHCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCCC
GWSAGANVAGRSIMTTNDMPITYPPSFDALNLFPHQLNPHFISGKPAGHNGESREERLAE
CCCCCCCCCCCEEEEECCCCEEECCCCCCEECCCCCCCCEEECCCCCCCCCCHHHHHHHE
FLIVNPTANVYALPEGTALHIQGQQARVLGQHDVLLFSENMQLATLPVNSVFDY
EEEECCCCCEEECCCCCEEEEECCCEEEECCCEEEEEECCCEEEEECHHHHCCC
>Mature Secondary Structure
MKNMLLMSGSKYQNTDYLVHTLPWLQDFLADYQGKTVAFVPYAGVRQSYDEYELKVQKAL
CCCEEEECCCCCCCCCEEEEHHHHHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHHH
AELNVAILSVHRAEKHAEIIEKADVIAIGGGNTFCLLKGMYEHHLLPLIREKVQSGTPYF
HHHHHHEEEHHHHHHHHHHHHHCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCCC
GWSAGANVAGRSIMTTNDMPITYPPSFDALNLFPHQLNPHFISGKPAGHNGESREERLAE
CCCCCCCCCCCEEEEECCCCEEECCCCCCEECCCCCCCCEEECCCCCCCCCCHHHHHHHE
FLIVNPTANVYALPEGTALHIQGQQARVLGQHDVLLFSENMQLATLPVNSVFDY
EEEECCCCCEEECCCCCEEEEECCCEEEECCCEEEEEECCCEEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100