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The map label for this gene is lipA [H]

Identifier: 15603795

GI number: 15603795

Start: 2168964

End: 2169926

Strand: Direct

Name: lipA [H]

Synonym: PM1930

Alternate gene names: 15603795

Gene position: 2168964-2169926 (Clockwise)

Preceding gene: 15603794

Following gene: 15603797

Centisome position: 96.08

GC content: 41.33

Gene sequence:

>963_bases
ATGGGTACACCTTTTAAAATGGAACGTGGTGTCAAATACCGTGATGCTGCTAAAACGTCCATTATCCCGGTAAAAAATAT
CGATCCAAATCAGGAATTATTAAAAAAACCGGAATGGATGAAAATCAAACTCCCTGCGAATTCTGCCAAAATTGATAGCA
TTAAAAACGGCATGCGTCGTCATGGTTTACACTCAGTGTGTGAAGAAGCATCGTGCCCGAATTTACATGAATGTTTTAAT
CACGGAACAGCAACCTTCATGATTTTAGGGGCAATTTGTACTCGCCGTTGTCCTTTCTGTGATGTGGCGCATGGTAAGCC
TTTACCGCCAGATCCTGAAGAACCACGTAAGTTAGCGGAGACAATCCAAGACATGAAATTGCGTTATGTGGTGATTACTT
CTGTGGATCGTGATGACTTGCCTGATCGTGGTGCGGGTCACTTTGCAGAATGTGTAAAAGAAATTCGTCAGCTCAATCCA
AATATCAAAATTGAGATTTTGGTTCCTGATTTTCGTGGGCGAATTGAGCAAGCGTTGGATAAATTGAAAGACAATCCACC
AGATGTGTTTAACCATAATTTAGAAAATGTACCACGTTTATACCGTGAAATTCGTCCAGGAGCAGATTATCAATGGTCTT
TGAAATTGCTTAAAGATTTTAAAGCGATGTTTCCACATATTCCGACCAAATCAGGTTTAATGGTGGGGTTAGGTGAAACT
AATGAAGAGATTCTGCAAGTGATGCAAGATCTACGCGATCATGGTGTGACTATGTTAACCTTAGGACAATATTTACAACC
AAGTCGCCATCATTTACCTGTTGCACGTTATGTACCTCCGGCAGAGTTTGATGAGTTCCGTGATAAAGCACAAGCCATGG
GCTTTGAACACGCTGCTTGCGGTCCGTTTGTGCGTTCTTCTTATCATGCAGACTTGCAAGCCAAAGGCGAAATTGTGAAA
TAA

Upstream 100 bases:

>100_bases
GCACAATTATTAGGATATAATGCAACAAATTTTTAACATTTCTGAAAGTTGTTGAAAACCGACCGCACTTTCAAACGTCA
TTAGAAGTTAAGGAACCAAG

Downstream 100 bases:

>100_bases
TGCCTTGATAAAACTTGGAAAATAAAAAATGGAAAGGGCTTAACTTTCCATTTTTTCTTACCAGATTGTGCATGTTATCA
AAGAGGGTAAGCACGATTTA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MGTPFKMERGVKYRDAAKTSIIPVKNIDPNQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFN
HGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPRKLAETIQDMKLRYVVITSVDRDDLPDRGAGHFAECVKEIRQLNP
NIKIEILVPDFRGRIEQALDKLKDNPPDVFNHNLENVPRLYREIRPGADYQWSLKLLKDFKAMFPHIPTKSGLMVGLGET
NEEILQVMQDLRDHGVTMLTLGQYLQPSRHHLPVARYVPPAEFDEFRDKAQAMGFEHAACGPFVRSSYHADLQAKGEIVK

Sequences:

>Translated_320_residues
MGTPFKMERGVKYRDAAKTSIIPVKNIDPNQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFN
HGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPRKLAETIQDMKLRYVVITSVDRDDLPDRGAGHFAECVKEIRQLNP
NIKIEILVPDFRGRIEQALDKLKDNPPDVFNHNLENVPRLYREIRPGADYQWSLKLLKDFKAMFPHIPTKSGLMVGLGET
NEEILQVMQDLRDHGVTMLTLGQYLQPSRHHLPVARYVPPAEFDEFRDKAQAMGFEHAACGPFVRSSYHADLQAKGEIVK
>Mature_319_residues
GTPFKMERGVKYRDAAKTSIIPVKNIDPNQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFNH
GTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPRKLAETIQDMKLRYVVITSVDRDDLPDRGAGHFAECVKEIRQLNPN
IKIEILVPDFRGRIEQALDKLKDNPPDVFNHNLENVPRLYREIRPGADYQWSLKLLKDFKAMFPHIPTKSGLMVGLGETN
EEILQVMQDLRDHGVTMLTLGQYLQPSRHHLPVARYVPPAEFDEFRDKAQAMGFEHAACGPFVRSSYHADLQAKGEIVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=286, Percent_Identity=43.7062937062937, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=44.4, Blast_Score=214, Evalue=6e-56,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=75.7009345794392, Blast_Score=511, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=40.5594405594406, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=43.2343234323432, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI221513272, Length=289, Percent_Identity=45.6747404844291, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36349; Mature: 36218

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTPFKMERGVKYRDAAKTSIIPVKNIDPNQELLKKPEWMKIKLPANSAKIDSIKNGMRR
CCCCCCCCCCCCHHHHCCCCEEECCCCCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
HGLHSVCEEASCPNLHECFNHGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPRKLAE
HHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
TIQDMKLRYVVITSVDRDDLPDRGAGHFAECVKEIRQLNPNIKIEILVPDFRGRIEQALD
HHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
KLKDNPPDVFNHNLENVPRLYREIRPGADYQWSLKLLKDFKAMFPHIPTKSGLMVGLGET
HHCCCCCHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
NEEILQVMQDLRDHGVTMLTLGQYLQPSRHHLPVARYVPPAEFDEFRDKAQAMGFEHAAC
HHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHC
GPFVRSSYHADLQAKGEIVK
CHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GTPFKMERGVKYRDAAKTSIIPVKNIDPNQELLKKPEWMKIKLPANSAKIDSIKNGMRR
CCCCCCCCCCCHHHHCCCCEEECCCCCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
HGLHSVCEEASCPNLHECFNHGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPRKLAE
HHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
TIQDMKLRYVVITSVDRDDLPDRGAGHFAECVKEIRQLNPNIKIEILVPDFRGRIEQALD
HHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
KLKDNPPDVFNHNLENVPRLYREIRPGADYQWSLKLLKDFKAMFPHIPTKSGLMVGLGET
HHCCCCCHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
NEEILQVMQDLRDHGVTMLTLGQYLQPSRHHLPVARYVPPAEFDEFRDKAQAMGFEHAAC
HHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHC
GPFVRSSYHADLQAKGEIVK
CHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA