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The map label for this gene is lipB

Identifier: 15603794

GI number: 15603794

Start: 2168243

End: 2168899

Strand: Direct

Name: lipB

Synonym: PM1929

Alternate gene names: 15603794

Gene position: 2168243-2168899 (Clockwise)

Preceding gene: 15603793

Following gene: 15603795

Centisome position: 96.05

GC content: 41.1

Gene sequence:

>657_bases
ATGTCTAATCTATCCCCACTACTTGTTCGCCAACTTGGCATACAAGATTACCAAACGGTTTGGCAACAAATGCAAACCTT
TACTGATTCACGTGATGTGGATACACAAGATGAAATCTGGCTCGTTCAACACCCTGCGGTTTTTACCCAAGGGCAAGCAG
GTAAAGCAGAACATTTATTGCAACAAACGACTATTCCGGTGGTGCAATCTGATCGGGGTGGACAAATCACCTATCATGGC
TTAGGGCAACAAGTGATGTATGTGTTGGTTGATATTAAACGCCAAAAAGCCTTAGGTAATGAATTTAATGTACGCCAATT
AGTGAGCGCGCTTGAACAAGCGGTGGTGAATACCTTAGCAGATTATGGCATTCACGCTTATCCTAAAGCGGATGCACCCG
GTGTGTATATTGATGAGAAGAAGATTTGTTCTTTAGGCTTACGAATCCGTAAGGGTTGCTCTTTTCATGGTCTGGCGTTG
AATATTAATATGGATTTATCGCCATTCCACTTTATTAATCCTTGTGGTTATGCCGGTTTAGAAATGTGCCAGTTAGCGGA
TTTCATTGATCCTCAACAAGCAAGCTGTGAAAAAGTCGCACCGAAATTAGTTGCACACTTTGCACAATTATTAGGATATA
ATGCAACAAATTTTTAA

Upstream 100 bases:

>100_bases
GGTGTCTATTGATATCATTGCAGAGCATATTGAACAAGTCGAAGTGCTTTATGTTGAGCTTGCCAAAATTGATGGCGTCA
GAATGGTGCTATAACGAAAA

Downstream 100 bases:

>100_bases
CATTTCTGAAAGTTGTTGAAAACCGACCGCACTTTCAAACGTCATTAGAAGTTAAGGAACCAAGATGGGTACACCTTTTA
AAATGGAACGTGGTGTCAAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSNLSPLLVRQLGIQDYQTVWQQMQTFTDSRDVDTQDEIWLVQHPAVFTQGQAGKAEHLLQQTTIPVVQSDRGGQITYHG
LGQQVMYVLVDIKRQKALGNEFNVRQLVSALEQAVVNTLADYGIHAYPKADAPGVYIDEKKICSLGLRIRKGCSFHGLAL
NINMDLSPFHFINPCGYAGLEMCQLADFIDPQQASCEKVAPKLVAHFAQLLGYNATNF

Sequences:

>Translated_218_residues
MSNLSPLLVRQLGIQDYQTVWQQMQTFTDSRDVDTQDEIWLVQHPAVFTQGQAGKAEHLLQQTTIPVVQSDRGGQITYHG
LGQQVMYVLVDIKRQKALGNEFNVRQLVSALEQAVVNTLADYGIHAYPKADAPGVYIDEKKICSLGLRIRKGCSFHGLAL
NINMDLSPFHFINPCGYAGLEMCQLADFIDPQQASCEKVAPKLVAHFAQLLGYNATNF
>Mature_217_residues
SNLSPLLVRQLGIQDYQTVWQQMQTFTDSRDVDTQDEIWLVQHPAVFTQGQAGKAEHLLQQTTIPVVQSDRGGQITYHGL
GQQVMYVLVDIKRQKALGNEFNVRQLVSALEQAVVNTLADYGIHAYPKADAPGVYIDEKKICSLGLRIRKGCSFHGLALN
INMDLSPFHFINPCGYAGLEMCQLADFIDPQQASCEKVAPKLVAHFAQLLGYNATNF

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=207, Percent_Identity=28.5024154589372, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI87081767, Length=205, Percent_Identity=63.4146341463415, Blast_Score=273, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6323268, Length=157, Percent_Identity=31.2101910828025, Blast_Score=82, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21355483, Length=224, Percent_Identity=27.2321428571429, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_PASMU (P57977)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246868.1
- ProteinModelPortal:   P57977
- SMR:   P57977
- GeneID:   1245276
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1929
- NMPDR:   fig|272843.1.peg.1930
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- ProtClustDB:   PRK14342
- BioCyc:   PMUL272843:PM1929-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24280; Mature: 24149

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLSPLLVRQLGIQDYQTVWQQMQTFTDSRDVDTQDEIWLVQHPAVFTQGQAGKAEHLL
CCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEECCCCCHHHHHH
QQTTIPVVQSDRGGQITYHGLGQQVMYVLVDIKRQKALGNEFNVRQLVSALEQAVVNTLA
HHHCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
DYGIHAYPKADAPGVYIDEKKICSLGLRIRKGCSFHGLALNINMDLSPFHFINPCGYAGL
HCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHH
EMCQLADFIDPQQASCEKVAPKLVAHFAQLLGYNATNF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SNLSPLLVRQLGIQDYQTVWQQMQTFTDSRDVDTQDEIWLVQHPAVFTQGQAGKAEHLL
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEECCCCCHHHHHH
QQTTIPVVQSDRGGQITYHGLGQQVMYVLVDIKRQKALGNEFNVRQLVSALEQAVVNTLA
HHHCCCEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
DYGIHAYPKADAPGVYIDEKKICSLGLRIRKGCSFHGLALNINMDLSPFHFINPCGYAGL
HCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHH
EMCQLADFIDPQQASCEKVAPKLVAHFAQLLGYNATNF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100