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The map label for this gene is bioD

Identifier: 15603769

GI number: 15603769

Start: 2143849

End: 2144490

Strand: Direct

Name: bioD

Synonym: PM1904

Alternate gene names: 15603769

Gene position: 2143849-2144490 (Clockwise)

Preceding gene: 15603768

Following gene: 15603770

Centisome position: 94.97

GC content: 40.03

Gene sequence:

>642_bases
ATGGCAAAGGTTTTTTTTATCACTGGCATTGACACCGATATTGGTAAAACCATCGCCACAGGTTGGTACGCCAAGAAATT
GATGCAACAAGGCGCGAGTGTCATCACGCAAAAGATGATCCAAACAGGCTGCCGAGGAATTGCCGAAGATTTACTTACAC
ACCGAAAAATACAAGGTATTGAGCTTACAGAAGAAGATAAGCAAGGTATCACTTGTCCTTATGTGTTTGATTATCCTTGC
TCACCGCATTTAGCCGCGAAATTAGAACAGCGCACGATCGAACGTAAAAAAATTGAAACCTGCACCGCACTTTTGTGTGA
AAAATATGATTATGTATTATTGGAAGGGGCGGGCGGCTTGTGTGTACCTTATAATGAGGAAGAAACCACCCTTGATTATC
TTTGTCAGCATCAATACCCCGTGATTTTAGTCACCTCTGGCAAGCTAGGTAGTATTAATCATACCTTATTGAGTTTACAA
GTACTCAACTCCAAAAGAGTAAGTGTGCATGCGGTGATATACAACTTGTATCCGGAAACAGATCAAGTCATTAGCCAAGA
AACACAACATTTTTTACGTCGTTATTTAGAGAAATACTCGCCTAATACCTTATTTGAAGTAATGGATTTGATTTCGGTCT
AA

Upstream 100 bases:

>100_bases
AATATCAACAACATTACTGTAACGCACAACACAAAGTAGAACTCACTTACACGCCGATGTATGTGTTGGCGCGTTTAACC
TAACCTTAGAAGGAAAAAGT

Downstream 100 bases:

>100_bases
GATATATTCTTGAGCAAATTGCACTTGAAATGGAGTGTTGTTTTGCCTAAGATGAAAGCGTCTCGGATTAAGACATCATT
GAGTTTAGGTTGTCTCATCA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase 2; DTBS 2; Dethiobiotin synthase 2

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MAKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGCRGIAEDLLTHRKIQGIELTEEDKQGITCPYVFDYPC
SPHLAAKLEQRTIERKKIETCTALLCEKYDYVLLEGAGGLCVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQ
VLNSKRVSVHAVIYNLYPETDQVISQETQHFLRRYLEKYSPNTLFEVMDLISV

Sequences:

>Translated_213_residues
MAKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGCRGIAEDLLTHRKIQGIELTEEDKQGITCPYVFDYPC
SPHLAAKLEQRTIERKKIETCTALLCEKYDYVLLEGAGGLCVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQ
VLNSKRVSVHAVIYNLYPETDQVISQETQHFLRRYLEKYSPNTLFEVMDLISV
>Mature_212_residues
AKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGCRGIAEDLLTHRKIQGIELTEEDKQGITCPYVFDYPCS
PHLAAKLEQRTIERKKIETCTALLCEKYDYVLLEGAGGLCVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQV
LNSKRVSVHAVIYNLYPETDQVISQETQHFLRRYLEKYSPNTLFEVMDLISV

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=179, Percent_Identity=32.4022346368715, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI87081944, Length=191, Percent_Identity=27.7486910994764, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD2_PASMU (Q9CJT7)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246843.1
- ProteinModelPortal:   Q9CJT7
- SMR:   Q9CJT7
- GeneID:   1245251
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1904
- NMPDR:   fig|272843.1.peg.1905
- HOGENOM:   HBG650065
- OMA:   LNNYNHE
- ProtClustDB:   PRK00090
- BioCyc:   PMUL272843:PM1904-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 24145; Mature: 24014

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGCRGIAEDLLTHRKIQGI
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELTEEDKQGITCPYVFDYPCSPHLAAKLEQRTIERKKIETCTALLCEKYDYVLLEGAGGL
CCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
CVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQVLNSKRVSVHAVIYNLYPET
EECCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHEEEECCCCCEEEEEEEEECCCCH
DQVISQETQHFLRRYLEKYSPNTLFEVMDLISV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGCRGIAEDLLTHRKIQGI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELTEEDKQGITCPYVFDYPCSPHLAAKLEQRTIERKKIETCTALLCEKYDYVLLEGAGGL
CCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
CVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQVLNSKRVSVHAVIYNLYPET
EECCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHEEEECCCCCEEEEEEEEECCCCH
DQVISQETQHFLRRYLEKYSPNTLFEVMDLISV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100