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The map label for this gene is rumA

Identifier: 15603731

GI number: 15603731

Start: 2108148

End: 2109461

Strand: Reverse

Name: rumA

Synonym: PM1866

Alternate gene names: 15603731

Gene position: 2109461-2108148 (Counterclockwise)

Preceding gene: 15603732

Following gene: 15603730

Centisome position: 93.44

GC content: 42.09

Gene sequence:

>1314_bases
ATGGTATTACACTATACCCCTCATCAATCCGCCCCACGCAACACAACATTCGTTGCGGAAATTCTTGATCTTGATTATCA
AGGACGTGGTGTAGCCAAAGTACAAGGCAAAACGTGGTTCATTGAAAATGCACTGCCACAAGAAAAAGTGGAAGTGCGCA
TTGTCGATGAAAAACGCCATTATGGTCATGGGATCAGCTGCAAAATTCTCACTCCACATCCAGATCGCCAGTCAGCAAAA
TGTGCTTACTATGCCCAGTGCGGTGGTTGCCAAAGTCAACATATTCCAATTGACATGCAACGTCAGGCTAAACAACAAGC
CTTATTCCAACGCTTACAACAATTACAACCTCAAGCGACCTTCATGCCCATGATCGTCGCAGCGCCTTGGCATTATCGCC
GTCGTGTGCGTTTAAGCGTGCGGTTTCATCCCAAAAGCAAACAACTTGCGATGGGTTTGCGTCAGAGAAATACTCAACAA
ATCGTGAATCTGCAGCATTGTGATGTGCTTGAAATCCCCTTAAGTCAACTCTTACCTAAACTACATTTGTTGTTTTCAAC
ATGGTCCCTGCCTAAAAACCTAGGGCATGTGGAGTTAGTGCATGCGGATAATGGAATTGCGATGTTATTACGCCATACAG
GAAATTTAGCGCAAACTGACCGCACTTTATTAACCAATTTTGCGCAACAAGAAAACTTAATGTTGTTTGTACAAGATGAT
CAACAGATCACCCAACTACATGGCGAGGCACCTTACTACATACTACGCGATGGCACCAAATTACAGTTTGATATCCGTGA
CTTTATCCAAGTGAATGCTGTTGTAAATCAGAAAATGATTGATACTGCTCTTGAGTGGTTGGAACTCACATCGAACGATA
ACGTATTAGATTTGTTTTGTGGTATGGGAAACTTCACCCTCCCAATCAGTCGTCAGGTCAATCAGGTTGTGGGCATTGAA
GGCGTAGGAGAAATGGTGGAGAAAGCAAAACGAAATGCGGAACAAAATCAATGTGATAATGTCCAATTCTATCAGGCGAA
TTTAGATCAACCTTTTGTGCAACAACATTGGGCGAGCCAACATTTTAATAAAATTTTACTGGACCCACCACGTACAGGCG
CGGCATTTGCCTTACATGCCTTATGTGAATTGGGCGCAGAAAAAATCTTATATGTTTCCTGCAATCCTGCTACATTAGTA
CGTGATACAGCGATTTTATTACAATTTAACTACCGACTTAAGAAAGTCGCAATGATCGATATGTTCCCCAATACAGGACA
TTTAGAATCCATCAGTTTATTTGAAAAAGAATAG

Upstream 100 bases:

>100_bases
TAAACGTTTCACTCGCATCGCGCTTAAACAGTATTTAGGGAATAAGCCCTTAAAAAGCCGAGAATTATTTACTCAATATA
TGTTGTATCAAAAATAAACT

Downstream 100 bases:

>100_bases
GATAGATTATGGTTGCAGTGCGAGGATCACACTTACTCAATCCAAAAGATTTTGTTATTGAAACCTGGTGTGCCAGTTTA
GAGGTTTCACCAGAAGTACG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MVLHYTPHQSAPRNTTFVAEILDLDYQGRGVAKVQGKTWFIENALPQEKVEVRIVDEKRHYGHGISCKILTPHPDRQSAK
CAYYAQCGGCQSQHIPIDMQRQAKQQALFQRLQQLQPQATFMPMIVAAPWHYRRRVRLSVRFHPKSKQLAMGLRQRNTQQ
IVNLQHCDVLEIPLSQLLPKLHLLFSTWSLPKNLGHVELVHADNGIAMLLRHTGNLAQTDRTLLTNFAQQENLMLFVQDD
QQITQLHGEAPYYILRDGTKLQFDIRDFIQVNAVVNQKMIDTALEWLELTSNDNVLDLFCGMGNFTLPISRQVNQVVGIE
GVGEMVEKAKRNAEQNQCDNVQFYQANLDQPFVQQHWASQHFNKILLDPPRTGAAFALHALCELGAEKILYVSCNPATLV
RDTAILLQFNYRLKKVAMIDMFPNTGHLESISLFEKE

Sequences:

>Translated_437_residues
MVLHYTPHQSAPRNTTFVAEILDLDYQGRGVAKVQGKTWFIENALPQEKVEVRIVDEKRHYGHGISCKILTPHPDRQSAK
CAYYAQCGGCQSQHIPIDMQRQAKQQALFQRLQQLQPQATFMPMIVAAPWHYRRRVRLSVRFHPKSKQLAMGLRQRNTQQ
IVNLQHCDVLEIPLSQLLPKLHLLFSTWSLPKNLGHVELVHADNGIAMLLRHTGNLAQTDRTLLTNFAQQENLMLFVQDD
QQITQLHGEAPYYILRDGTKLQFDIRDFIQVNAVVNQKMIDTALEWLELTSNDNVLDLFCGMGNFTLPISRQVNQVVGIE
GVGEMVEKAKRNAEQNQCDNVQFYQANLDQPFVQQHWASQHFNKILLDPPRTGAAFALHALCELGAEKILYVSCNPATLV
RDTAILLQFNYRLKKVAMIDMFPNTGHLESISLFEKE
>Mature_437_residues
MVLHYTPHQSAPRNTTFVAEILDLDYQGRGVAKVQGKTWFIENALPQEKVEVRIVDEKRHYGHGISCKILTPHPDRQSAK
CAYYAQCGGCQSQHIPIDMQRQAKQQALFQRLQQLQPQATFMPMIVAAPWHYRRRVRLSVRFHPKSKQLAMGLRQRNTQQ
IVNLQHCDVLEIPLSQLLPKLHLLFSTWSLPKNLGHVELVHADNGIAMLLRHTGNLAQTDRTLLTNFAQQENLMLFVQDD
QQITQLHGEAPYYILRDGTKLQFDIRDFIQVNAVVNQKMIDTALEWLELTSNDNVLDLFCGMGNFTLPISRQVNQVVGIE
GVGEMVEKAKRNAEQNQCDNVQFYQANLDQPFVQQHWASQHFNKILLDPPRTGAAFALHALCELGAEKILYVSCNPATLV
RDTAILLQFNYRLKKVAMIDMFPNTGHLESISLFEKE

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=195, Percent_Identity=32.3076923076923, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI51173878, Length=195, Percent_Identity=32.3076923076923, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789148, Length=432, Percent_Identity=44.6759259259259, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1790403, Length=175, Percent_Identity=32, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787083, Length=392, Percent_Identity=25, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24666579, Length=197, Percent_Identity=30.9644670050761, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_PASMU (Q9CJX3)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246805.1
- ProteinModelPortal:   Q9CJX3
- SMR:   Q9CJX3
- GeneID:   1245213
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1866
- NMPDR:   fig|272843.1.peg.1867
- HOGENOM:   HBG690163
- OMA:   VVFVEGA
- ProtClustDB:   PRK13168
- BioCyc:   PMUL272843:PM1866-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 50022; Mature: 50022

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 394-394 BINDING 270-270 BINDING 299-299 BINDING 304-304 BINDING 320-320 BINDING 347-347 BINDING 368-368

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLHYTPHQSAPRNTTFVAEILDLDYQGRGVAKVQGKTWFIENALPQEKVEVRIVDEKRH
CEEEECCCCCCCCCCEEEEHHHCCCCCCCCEEEECCCEEEEECCCCCCCEEEEEEECCCC
YGHGISCKILTPHPDRQSAKCAYYAQCGGCQSQHIPIDMQRQAKQQALFQRLQQLQPQAT
CCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
FMPMIVAAPWHYRRRVRLSVRFHPKSKQLAMGLRQRNTQQIVNLQHCDVLEIPLSQLLPK
HCCEEEECCCCCEEEEEEEEEECCCHHHHHHHHHHCCHHHHHCCCCCCEEECCHHHHHHH
LHLLFSTWSLPKNLGHVELVHADNGIAMLLRHTGNLAQTDRTLLTNFAQQENLMLFVQDD
HHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECC
QQITQLHGEAPYYILRDGTKLQFDIRDFIQVNAVVNQKMIDTALEWLELTSNDNVLDLFC
HHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GMGNFTLPISRQVNQVVGIEGVGEMVEKAKRNAEQNQCDNVQFYQANLDQPFVQQHWASQ
CCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHH
HFNKILLDPPRTGAAFALHALCELGAEKILYVSCNPATLVRDTAILLQFNYRLKKVAMID
HCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHCEEEEEEECCCEEEEEEEE
MFPNTGHLESISLFEKE
ECCCCCCCCEECCCCCC
>Mature Secondary Structure
MVLHYTPHQSAPRNTTFVAEILDLDYQGRGVAKVQGKTWFIENALPQEKVEVRIVDEKRH
CEEEECCCCCCCCCCEEEEHHHCCCCCCCCEEEECCCEEEEECCCCCCCEEEEEEECCCC
YGHGISCKILTPHPDRQSAKCAYYAQCGGCQSQHIPIDMQRQAKQQALFQRLQQLQPQAT
CCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
FMPMIVAAPWHYRRRVRLSVRFHPKSKQLAMGLRQRNTQQIVNLQHCDVLEIPLSQLLPK
HCCEEEECCCCCEEEEEEEEEECCCHHHHHHHHHHCCHHHHHCCCCCCEEECCHHHHHHH
LHLLFSTWSLPKNLGHVELVHADNGIAMLLRHTGNLAQTDRTLLTNFAQQENLMLFVQDD
HHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECC
QQITQLHGEAPYYILRDGTKLQFDIRDFIQVNAVVNQKMIDTALEWLELTSNDNVLDLFC
HHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GMGNFTLPISRQVNQVVGIEGVGEMVEKAKRNAEQNQCDNVQFYQANLDQPFVQQHWASQ
CCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHH
HFNKILLDPPRTGAAFALHALCELGAEKILYVSCNPATLVRDTAILLQFNYRLKKVAMID
HCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHCEEEEEEECCCEEEEEEEE
MFPNTGHLESISLFEKE
ECCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100