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The map label for this gene is pgk

Identifier: 15603725

GI number: 15603725

Start: 2101833

End: 2102996

Strand: Direct

Name: pgk

Synonym: PM1860

Alternate gene names: 15603725

Gene position: 2101833-2102996 (Clockwise)

Preceding gene: 15603724

Following gene: 15603726

Centisome position: 93.1

GC content: 42.61

Gene sequence:

>1164_bases
ATGTCAGTAATCAAAATGACAGACTTGGATTTGAGCGGCAAACGTGTGTTTATTCGTGCTGATTTAAATGTGCCAGTCAA
AGATGGCAAAGTGACTTCTGATGCACGTATCCGTGCGACCATCCCTACCTTGAAACTTGCGCTTGAAAAAGGCGCAAAAG
TGATGGTGACTTCACACTTAGGTCGCCCAACAGAAGGCGAATTTGATGAAGCAAACTCACTGAAACCTGTAGTTGATTAC
TTAAATGCAAATTTAGATGTGCCTGTTCGTTTAGTACGCGATTATTTAGACGGTGTGGACGTTAACCAAGGCGAAATCGT
GGTATTAGAAAACGTGCGCATTAACAAAGGTGAGAAGAAAAACGATCCTGAATTAGGCAAAAAATATGCCGCACTTTGCG
ATGTCTTTGTGATGGATGCCTTTGGTACTGCACACCGTGCGCAAGCCTCGACTTACGGTGTGGCTGAATATGCGCCAGTG
GCTTGTGCAGGTCCATTATTAGCGGCTGAATTAGATGCTTTAGGCAAAGCCTTAAAAGAACCTGCGCGTCCAATGGTGGC
GATTGTAGGGGGGTCGAAAGTCTCAACCAAATTAGAAGTGTTAAATTCCTTATCTAAAATTGCGGACCAAATTATTGTGG
GCGGGGGTATCGCCAATACCTTCATTGCTGCAGCAGGTCATAACGTCGGTAAATCCTTATATGAAGAAGATTTAATTCCT
GTGGCGAAAAACTTAGCAGCAAGCACAGATATTCCTGTACCCGTTGATGTTCGCGTTGGGTTAGAATTCTCTGAAACTGC
AGCAGCAACAGAAAAAGCCGTCAATGAAGTGAAAGATGATGAATCCATTTTTGATATTGGTGATAAATCAGCAGAACAAC
TCGCGGAAATCATCAAAAATGCGAAAACGATTCTTTGGAATGGTCCAGTGGGCGTGTTTGAGTTCCCGAATTTCCGTAAA
GGGACTGAAATCATTTCTCACGCAATCGCTAATAGCGAAGGTTTCTCAATTGCAGGTGGTGGTGACACATTAGCGGCGAT
TGACCTATTCGGTATCAAAGATAAAATTTCTTATATTTCAACAGGTGGTGGTGCATTCTTAGAATTCGTTGAAGGTAAAG
TATTACCAGCAGTAGAAATCTTAGAAAAACGTGCAAATGGTTAA

Upstream 100 bases:

>100_bases
AATAAAAATTATTTGATCTAACGCAGTACAGCCTAGTTAGAAAGGGGTATAATGCGTTTAGTTTTCTCATTTTTTAATTT
AACCAGTAAGAGGATAAAAT

Downstream 100 bases:

>100_bases
TTGGGTAGGGGCAGGCTTGTGTAGTCGCCCCTCTCTATTTTGAATGAGAATAAGCATTAGGCTTATCAAGAAACGAAATT
TCACTCTAATCAAATAAATA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSVIKMTDLDLSGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHLGRPTEGEFDEANSLKPVVDY
LNANLDVPVRLVRDYLDGVDVNQGEIVVLENVRINKGEKKNDPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEYAPV
ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEEDLIP
VAKNLAASTDIPVPVDVRVGLEFSETAAATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNAKTILWNGPVGVFEFPNFRK
GTEIISHAIANSEGFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG

Sequences:

>Translated_387_residues
MSVIKMTDLDLSGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHLGRPTEGEFDEANSLKPVVDY
LNANLDVPVRLVRDYLDGVDVNQGEIVVLENVRINKGEKKNDPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEYAPV
ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEEDLIP
VAKNLAASTDIPVPVDVRVGLEFSETAAATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNAKTILWNGPVGVFEFPNFRK
GTEIISHAIANSEGFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG
>Mature_386_residues
SVIKMTDLDLSGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHLGRPTEGEFDEANSLKPVVDYL
NANLDVPVRLVRDYLDGVDVNQGEIVVLENVRINKGEKKNDPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEYAPVA
CAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEEDLIPV
AKNLAASTDIPVPVDVRVGLEFSETAAATEKAVNEVKDDESIFDIGDKSAEQLAEIIKNAKTILWNGPVGVFEFPNFRKG
TEIISHAIANSEGFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=37.8640776699029, Blast_Score=245, Evalue=6e-65,
Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=37.2262773722628, Blast_Score=242, Evalue=5e-64,
Organism=Escherichia coli, GI1789294, Length=385, Percent_Identity=81.5584415584416, Blast_Score=633, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=39.9515738498789, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6319857, Length=419, Percent_Identity=38.4248210023866, Blast_Score=236, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=38.0145278450363, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI17136394, Length=410, Percent_Identity=39.0243902439024, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_PASMU (P57973)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246799.1
- ProteinModelPortal:   P57973
- SMR:   P57973
- PRIDE:   P57973
- GeneID:   1245207
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1860
- NMPDR:   fig|272843.1.peg.1861
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   PMUL272843:PM1860-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41030; Mature: 40899

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 113-113 BINDING 146-146 BINDING 197-197 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIKMTDLDLSGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHL
CCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCEEEEEECC
GRPTEGEFDEANSLKPVVDYLNANLDVPVRLVRDYLDGVDVNQGEIVVLENVRINKGEKK
CCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEECEEECCCCCC
NDPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEYAPVACAGPLLAAELDALGKALKE
CCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHCCCHHHCCHHHHHHHHHHHHHHHC
PARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEEDLIP
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCHHHHHHHHHHHHH
VAKNLAASTDIPVPVDVRVGLEFSETAAATEKAVNEVKDDESIFDIGDKSAEQLAEIIKN
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHC
AKTILWNGPVGVFEFPNFRKGTEIISHAIANSEGFSIAGGGDTLAAIDLFGIKDKISYIS
CCEEEECCCCCCEECCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEEEEECCHHHHHEEE
TGGGAFLEFVEGKVLPAVEILEKRANG
CCCHHHHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SVIKMTDLDLSGKRVFIRADLNVPVKDGKVTSDARIRATIPTLKLALEKGAKVMVTSHL
CEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCEEEEEECC
GRPTEGEFDEANSLKPVVDYLNANLDVPVRLVRDYLDGVDVNQGEIVVLENVRINKGEKK
CCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEECEEECCCCCC
NDPELGKKYAALCDVFVMDAFGTAHRAQASTYGVAEYAPVACAGPLLAAELDALGKALKE
CCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHCCCHHHCCHHHHHHHHHHHHHHHC
PARPMVAIVGGSKVSTKLEVLNSLSKIADQIIVGGGIANTFIAAAGHNVGKSLYEEDLIP
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCHHHHHHHHHHHHH
VAKNLAASTDIPVPVDVRVGLEFSETAAATEKAVNEVKDDESIFDIGDKSAEQLAEIIKN
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHC
AKTILWNGPVGVFEFPNFRKGTEIISHAIANSEGFSIAGGGDTLAAIDLFGIKDKISYIS
CCEEEECCCCCCEECCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEEEEECCHHHHHEEE
TGGGAFLEFVEGKVLPAVEILEKRANG
CCCHHHHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100