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The map label for this gene is ptsB

Identifier: 15603711

GI number: 15603711

Start: 2085618

End: 2087042

Strand: Reverse

Name: ptsB

Synonym: PM1846

Alternate gene names: 15603711

Gene position: 2087042-2085618 (Counterclockwise)

Preceding gene: 15603712

Following gene: 15603710

Centisome position: 92.45

GC content: 43.58

Gene sequence:

>1425_bases
ATGAACTTCTCGCAAATCGCCCAACAAGTGATTGACAAACTCGGTGGTAAAGACAATATCTCTGCTGCGGCACATTGTGC
TACCCGCTTGAGAATCGTTGTAAAAAATGAAAACTTGATTGATAAAAAAGGCATTGAGAATATTGAAGGTGTCAAAGGTC
AATTTGCCGTAGCAGGACAATACCAAATTATTTTTGGTTCTGGCACAGTCAATAAAGTCTATGCAGCGTTAAGCAAGTTG
TTAGGTATCGGTGATATGACGACTTCTGAAGTCGCTGCCGCTGGTACAGAAAAACAAGGGTTATTACAACGCTTAGTGAA
AGGTCTAGCTGATATTTTCGTCCCAATTATTCCTGCCATCGTTGCCGGTGGTTTGTTAATGGGTATTCACAGTATGTTGA
CAGCAAAAGGTTTCTTCGTTGAAGAAAAAAATGTTGTCGATCTGTATCCCGCAATAGCTGATTTAGTGGATTTTATTAAT
ACCATTGCAAATGCACCTTTTGTCTTCTTACCTGTATTACTTGGTTTTTCCGCCACTCGAAAATTTGGCGGTAATCCATT
CTTAGGAGCGGCACTTGGCATGCTATTAGTGCACCCAGCACTTTCTGATGGCTGGAATTATGCACTGACACTCGCTAAAG
GCAATATTCAATATTGGCATATCTTTGGTTTAGAAATTGAACGTGTCGGCTACCAAGGTACAGTTATCCCCGTTCTTGTC
GCCTCTTGGGTCTTAGCAACCTTGGAGAAAAACCTACGTAAAGTCGTTCCGTCATTTCTTGATAACTTAATTACCCCACT
TTTTGCATTATTTATAACGGGCTTACTCGCTTTTACCGTAATAGGTCCTATCGGGCGTGAAGCGGGGTCTTTAATTAGTA
CGGGCTTGACCTGGTTGTATGACACATTAGGATTCGTGGGGGGTGCCATTTTCGGTACACTTTACGCACCCATTGTTATC
ACAGGTATGCACCAAACATTTATCGCTGTCGAAACACAATTGCTTGCTGAGGTGGCTCGCACTGGAGGTACCTTTATCTT
CCCAATCGCCGCGATGTCAAATATTGCTCAAGGTGCAGCTTGTTTGGGCGCCGCTTATGTGATGAAAGATGCCAAAGTAA
GAGGGATCGCCGTACCTTCTGGCATCTCAGCCTTGTTAGGGATTACTGAGCCCGCCATGTTCGGTGTCAACTTACGCTAT
CGCTATCCTTTCATATCTGCCATGATCGGTGCTGGCATTTCCAGTGCGGTCATTGCCTTGTTTAATGTTAAAGCCATTGC
TTTAGGCGCTGCGGGCTTGCCGGGTATCCCCTCAATTAAGCCGGATAGTTTAGCAATGTATTGTGTCGGTATGCTTATTT
CCGCATCGATTGCATTTACTCTCACCGTCATTTTAGGTAAAAGAGCACAACTAAAAGCAGAATAA

Upstream 100 bases:

>100_bases
TAAAACGTTTTAGTTAAATGCACTTTTATAACTAAAACGTTTTAGTTAAAATAAAATTACACACTTTTTCTACATCAACA
CAACTTTATAGGTGGTGACC

Downstream 100 bases:

>100_bases
GACGATTGTTGCGTGTATAAAAATGCCGTGATAGAAAAAAATCACGGCATTTTTCTTTTTAAAAGCGTATGATATCCGCA
TATAGGATGCTCATTTTTAT

Product: PtsB

Products: NA

Alternate protein names: EIIBC-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MNFSQIAQQVIDKLGGKDNISAAAHCATRLRIVVKNENLIDKKGIENIEGVKGQFAVAGQYQIIFGSGTVNKVYAALSKL
LGIGDMTTSEVAAAGTEKQGLLQRLVKGLADIFVPIIPAIVAGGLLMGIHSMLTAKGFFVEEKNVVDLYPAIADLVDFIN
TIANAPFVFLPVLLGFSATRKFGGNPFLGAALGMLLVHPALSDGWNYALTLAKGNIQYWHIFGLEIERVGYQGTVIPVLV
ASWVLATLEKNLRKVVPSFLDNLITPLFALFITGLLAFTVIGPIGREAGSLISTGLTWLYDTLGFVGGAIFGTLYAPIVI
TGMHQTFIAVETQLLAEVARTGGTFIFPIAAMSNIAQGAACLGAAYVMKDAKVRGIAVPSGISALLGITEPAMFGVNLRY
RYPFISAMIGAGISSAVIALFNVKAIALGAAGLPGIPSIKPDSLAMYCVGMLISASIAFTLTVILGKRAQLKAE

Sequences:

>Translated_474_residues
MNFSQIAQQVIDKLGGKDNISAAAHCATRLRIVVKNENLIDKKGIENIEGVKGQFAVAGQYQIIFGSGTVNKVYAALSKL
LGIGDMTTSEVAAAGTEKQGLLQRLVKGLADIFVPIIPAIVAGGLLMGIHSMLTAKGFFVEEKNVVDLYPAIADLVDFIN
TIANAPFVFLPVLLGFSATRKFGGNPFLGAALGMLLVHPALSDGWNYALTLAKGNIQYWHIFGLEIERVGYQGTVIPVLV
ASWVLATLEKNLRKVVPSFLDNLITPLFALFITGLLAFTVIGPIGREAGSLISTGLTWLYDTLGFVGGAIFGTLYAPIVI
TGMHQTFIAVETQLLAEVARTGGTFIFPIAAMSNIAQGAACLGAAYVMKDAKVRGIAVPSGISALLGITEPAMFGVNLRY
RYPFISAMIGAGISSAVIALFNVKAIALGAAGLPGIPSIKPDSLAMYCVGMLISASIAFTLTVILGKRAQLKAE
>Mature_474_residues
MNFSQIAQQVIDKLGGKDNISAAAHCATRLRIVVKNENLIDKKGIENIEGVKGQFAVAGQYQIIFGSGTVNKVYAALSKL
LGIGDMTTSEVAAAGTEKQGLLQRLVKGLADIFVPIIPAIVAGGLLMGIHSMLTAKGFFVEEKNVVDLYPAIADLVDFIN
TIANAPFVFLPVLLGFSATRKFGGNPFLGAALGMLLVHPALSDGWNYALTLAKGNIQYWHIFGLEIERVGYQGTVIPVLV
ASWVLATLEKNLRKVVPSFLDNLITPLFALFITGLLAFTVIGPIGREAGSLISTGLTWLYDTLGFVGGAIFGTLYAPIVI
TGMHQTFIAVETQLLAEVARTGGTFIFPIAAMSNIAQGAACLGAAYVMKDAKVRGIAVPSGISALLGITEPAMFGVNLRY
RYPFISAMIGAGISSAVIALFNVKAIALGAAGLPGIPSIKPDSLAMYCVGMLISASIAFTLTVILGKRAQLKAE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain

Homologues:

Organism=Escherichia coli, GI2367362, Length=470, Percent_Identity=37.0212765957447, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI48994906, Length=478, Percent_Identity=31.3807531380753, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1790159, Length=467, Percent_Identity=28.051391862955, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1788769, Length=493, Percent_Identity=30.8316430020284, Blast_Score=178, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTSBC_PASMU (Q9CJZ2)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246785.1
- ProteinModelPortal:   Q9CJZ2
- GeneID:   1245193
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1846
- NMPDR:   fig|272843.1.peg.1847
- HOGENOM:   HBG673521
- OMA:   VITGMHQ
- ProtClustDB:   CLSK870636
- BioCyc:   PMUL272843:PM1846-MONOMER
- BRENDA:   2.7.1.69
- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973
- Gene3D:   G3DSA:3.30.1360.60
- TIGRFAMs:   TIGR00826
- TIGRFAMs:   TIGR01996

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC; SSF55604 PTS_EIIB

EC number: =2.7.1.69

Molecular weight: Translated: 49909; Mature: 49909

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS51098 PTS_EIIB_TYPE_1; PS01035 PTS_EIIB_TYPE_1_CYS; PS51103 PTS_EIIC_TYPE_1

Important sites: ACT_SITE 26-26

Signals:

None

Transmembrane regions:

HASH(0xf66188c)-; HASH(0xf951b00)-; HASH(0xd9e5114)-; HASH(0xf7257dc)-; HASH(0xdecbf04)-; HASH(0xf719778)-; HASH(0xf2bc69c)-; HASH(0xf5e0048)-; HASH(0xed4944c)-; HASH(0xf963344)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFSQIAQQVIDKLGGKDNISAAAHCATRLRIVVKNENLIDKKGIENIEGVKGQFAVAGQ
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEECE
YQIIFGSGTVNKVYAALSKLLGIGDMTTSEVAAAGTEKQGLLQRLVKGLADIFVPIIPAI
EEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VAGGLLMGIHSMLTAKGFFVEEKNVVDLYPAIADLVDFINTIANAPFVFLPVLLGFSATR
HHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
KFGGNPFLGAALGMLLVHPALSDGWNYALTLAKGNIQYWHIFGLEIERVGYQGTVIPVLV
HCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEEEEEEECCCCCCHHHHHH
ASWVLATLEKNLRKVVPSFLDNLITPLFALFITGLLAFTVIGPIGREAGSLISTGLTWLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
DTLGFVGGAIFGTLYAPIVITGMHQTFIAVETQLLAEVARTGGTFIFPIAAMSNIAQGAA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
CLGAAYVMKDAKVRGIAVPSGISALLGITEPAMFGVNLRYRYPFISAMIGAGISSAVIAL
HHHHHHHHHCCCCCEEECCCHHHHHHHCCCCHHCCCEEEEECHHHHHHHHCCHHHHHHHH
FNVKAIALGAAGLPGIPSIKPDSLAMYCVGMLISASIAFTLTVILGKRAQLKAE
HHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
>Mature Secondary Structure
MNFSQIAQQVIDKLGGKDNISAAAHCATRLRIVVKNENLIDKKGIENIEGVKGQFAVAGQ
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEECE
YQIIFGSGTVNKVYAALSKLLGIGDMTTSEVAAAGTEKQGLLQRLVKGLADIFVPIIPAI
EEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VAGGLLMGIHSMLTAKGFFVEEKNVVDLYPAIADLVDFINTIANAPFVFLPVLLGFSATR
HHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
KFGGNPFLGAALGMLLVHPALSDGWNYALTLAKGNIQYWHIFGLEIERVGYQGTVIPVLV
HCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEEEEEEECCCCCCHHHHHH
ASWVLATLEKNLRKVVPSFLDNLITPLFALFITGLLAFTVIGPIGREAGSLISTGLTWLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
DTLGFVGGAIFGTLYAPIVITGMHQTFIAVETQLLAEVARTGGTFIFPIAAMSNIAQGAA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
CLGAAYVMKDAKVRGIAVPSGISALLGITEPAMFGVNLRYRYPFISAMIGAGISSAVIAL
HHHHHHHHHCCCCCEEECCCHHHHHHHCCCCHHCCCEEEEECHHHHHHHHCCHHHHHHHH
FNVKAIALGAAGLPGIPSIKPDSLAMYCVGMLISASIAFTLTVILGKRAQLKAE
HHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100