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The map label for this gene is fruB [H]

Identifier: 15603662

GI number: 15603662

Start: 2023150

End: 2024295

Strand: Reverse

Name: fruB [H]

Synonym: PM1797

Alternate gene names: 15603662

Gene position: 2024295-2023150 (Counterclockwise)

Preceding gene: 15603663

Following gene: 15603661

Centisome position: 89.67

GC content: 42.76

Gene sequence:

>1146_bases
GTGTTTGAACTCACCGAAAACGACATTCACTTATCCTCTCAAGCCGTCAATAAAGATCAAGCCATTGAAATGGTTGCTCA
AGCACTTATACAATCAGGCTACGTAGAAGAAGGCTACTTAGCTGGTATGCTTGAACGTGAAGCGCAAATTACTACCTATT
TGGGCAATGGTATTGCGATTCCACATGGTTCTGTGACCACCCGCGCTTTAGTCAAAAAAACAGGCGTGAAAATTTTTCAG
TTTCCACAAGGCGTCGCTTGGGATAAAGATAATATCGCCTACCTTGTGATTGGACTTGCTGCTGATTCAGATGGACATCT
AGCTCTCCTGCGTCAATTAGCTCGTCTGCTTCACGATAAAGAAACGGTCAAACACTTAATCACAACACAGGATGTGTCCG
AATTTTATGCGCGCTTAATGGGGAAAAGTGCGACTAACCAACCTGATTTAATTAGCTTAGATCTTGATACGACTAGCCTA
TTAACCTTGACAGCACGTAATGCCGAAAAATTACAACAGTACCAAGCCGTCAATAACGAATTTGTTCGTGAAGTACTTGC
TAGCCCTGCGTTGCCTTTAGGGCAAGGGATTTGGTTGACGGATGCTACGCTCGGCAATCAAAAAAATGCTTGTGCTTTTA
GTCGTGCTAAAAACCCATTCTTACATAATGGTAAAAGCGTCCATGCCGTACTCACCATTGCAGCAATTGATAATAACATT
CATCCCGTCCTGATCCGTTTATTAGAACCAGAAGTTCAACAACAATTACTCACTGGCTCAAAAGAAACAATTGCCTCCTT
ATTAAACCTTGAGCACAAGCACGTAAACCAAACGGAAGGGACTGCGGGTTTAAATGAACAGATTGAACCTATTGAAGCGA
TTTTTACGCTGCGTAATCTACATGGGTTACACGCTCGTCCTGCCGCAATCTTAGTCAGTCATCTCAAAAAATATAATGCG
TCTATCGCCGTGCAAAATTTAACAACCAACAGTCAACTGATCAGCGCAAAAAGTTTGCTTAAAGTTATCGCACTGGGTGC
ACAACAAGGACATCGTTTACGCTTTGTTGCCACTGGTGCAGAAGCACAGCAAGCCATACAATGTGTAGGTGAGCTTATTG
ACAACCATTTAGGTGAGACAGCATGA

Upstream 100 bases:

>100_bases
ATGTGAAAATCAGCAGTTTTTTTCGCTTTTGGCAATAAAGAGAAAGATGACATTTTCTCGACATATGCATGATCTATAAT
GAAGAAGTACAGGAGTCGCC

Downstream 100 bases:

>100_bases
CAAAAGTACTGACCATTACACTAAATTCTGCCTACGACTTAGTAGGGCATCTCCCTCACCTAACACGAGGTGCTGTAAAC
CAGGTGGATTCTTTAGGACT

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Fructose PTS diphosphoryl transfer protein; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIIA-Fru; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MFELTENDIHLSSQAVNKDQAIEMVAQALIQSGYVEEGYLAGMLEREAQITTYLGNGIAIPHGSVTTRALVKKTGVKIFQ
FPQGVAWDKDNIAYLVIGLAADSDGHLALLRQLARLLHDKETVKHLITTQDVSEFYARLMGKSATNQPDLISLDLDTTSL
LTLTARNAEKLQQYQAVNNEFVREVLASPALPLGQGIWLTDATLGNQKNACAFSRAKNPFLHNGKSVHAVLTIAAIDNNI
HPVLIRLLEPEVQQQLLTGSKETIASLLNLEHKHVNQTEGTAGLNEQIEPIEAIFTLRNLHGLHARPAAILVSHLKKYNA
SIAVQNLTTNSQLISAKSLLKVIALGAQQGHRLRFVATGAEAQQAIQCVGELIDNHLGETA

Sequences:

>Translated_381_residues
MFELTENDIHLSSQAVNKDQAIEMVAQALIQSGYVEEGYLAGMLEREAQITTYLGNGIAIPHGSVTTRALVKKTGVKIFQ
FPQGVAWDKDNIAYLVIGLAADSDGHLALLRQLARLLHDKETVKHLITTQDVSEFYARLMGKSATNQPDLISLDLDTTSL
LTLTARNAEKLQQYQAVNNEFVREVLASPALPLGQGIWLTDATLGNQKNACAFSRAKNPFLHNGKSVHAVLTIAAIDNNI
HPVLIRLLEPEVQQQLLTGSKETIASLLNLEHKHVNQTEGTAGLNEQIEPIEAIFTLRNLHGLHARPAAILVSHLKKYNA
SIAVQNLTTNSQLISAKSLLKVIALGAQQGHRLRFVATGAEAQQAIQCVGELIDNHLGETA
>Mature_381_residues
MFELTENDIHLSSQAVNKDQAIEMVAQALIQSGYVEEGYLAGMLEREAQITTYLGNGIAIPHGSVTTRALVKKTGVKIFQ
FPQGVAWDKDNIAYLVIGLAADSDGHLALLRQLARLLHDKETVKHLITTQDVSEFYARLMGKSATNQPDLISLDLDTTSL
LTLTARNAEKLQQYQAVNNEFVREVLASPALPLGQGIWLTDATLGNQKNACAFSRAKNPFLHNGKSVHAVLTIAAIDNNI
HPVLIRLLEPEVQQQLLTGSKETIASLLNLEHKHVNQTEGTAGLNEQIEPIEAIFTLRNLHGLHARPAAILVSHLKKYNA
SIAVQNLTTNSQLISAKSLLKVIALGAQQGHRLRFVATGAEAQQAIQCVGELIDNHLGETA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=385, Percent_Identity=42.3376623376623, Blast_Score=297, Evalue=8e-82,
Organism=Escherichia coli, GI1790027, Length=144, Percent_Identity=38.8888888888889, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016258
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 41505; Mature: 41505

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFELTENDIHLSSQAVNKDQAIEMVAQALIQSGYVEEGYLAGMLEREAQITTYLGNGIAI
CCCCCCCCEECCHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEECCCEEC
PHGSVTTRALVKKTGVKIFQFPQGVAWDKDNIAYLVIGLAADSDGHLALLRQLARLLHDK
CCCCHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHH
ETVKHLITTQDVSEFYARLMGKSATNQPDLISLDLDTTSLLTLTARNAEKLQQYQAVNNE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHH
FVREVLASPALPLGQGIWLTDATLGNQKNACAFSRAKNPFLHNGKSVHAVLTIAAIDNNI
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEEEECCCC
HPVLIRLLEPEVQQQLLTGSKETIASLLNLEHKHVNQTEGTAGLNEQIEPIEAIFTLRNL
HHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
HGLHARPAAILVSHLKKYNASIAVQNLTTNSQLISAKSLLKVIALGAQQGHRLRFVATGA
CCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCH
EAQQAIQCVGELIDNHLGETA
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFELTENDIHLSSQAVNKDQAIEMVAQALIQSGYVEEGYLAGMLEREAQITTYLGNGIAI
CCCCCCCCEECCHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEECCCEEC
PHGSVTTRALVKKTGVKIFQFPQGVAWDKDNIAYLVIGLAADSDGHLALLRQLARLLHDK
CCCCHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHH
ETVKHLITTQDVSEFYARLMGKSATNQPDLISLDLDTTSLLTLTARNAEKLQQYQAVNNE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHH
FVREVLASPALPLGQGIWLTDATLGNQKNACAFSRAKNPFLHNGKSVHAVLTIAAIDNNI
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEEEECCCC
HPVLIRLLEPEVQQQLLTGSKETIASLLNLEHKHVNQTEGTAGLNEQIEPIEAIFTLRNL
HHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
HGLHARPAAILVSHLKKYNASIAVQNLTTNSQLISAKSLLKVIALGAQQGHRLRFVATGA
CCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCH
EAQQAIQCVGELIDNHLGETA
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 8763608 [H]