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The map label for this gene is fruA [H]

Identifier: 15603660

GI number: 15603660

Start: 2021041

End: 2022207

Strand: Reverse

Name: fruA [H]

Synonym: PM1795

Alternate gene names: 15603660

Gene position: 2022207-2021041 (Counterclockwise)

Preceding gene: 15603661

Following gene: 15603655

Centisome position: 89.58

GC content: 43.1

Gene sequence:

>1167_bases
ATGAACATTTTTCTCACACAAAAAGAAGACATTGGCAATGCAAAAGTATTCTTATTGCATCAAGTTATCACAGCAGCCGC
CCATCAACAAGGTCACCAAATCACCACATTAGATGAGGCAGATCTGGTCATTGTATTCGGTGAAGCAATACACAACCCTC
AATTTGTAGGTAAAAAATGCGTTATTGTCGATGCAGAAGAGGCGTTCAATGCTCCCGAAAATACTTTAACTAAGGCTTTC
GCAGAGGCAACTCCCTATGTTTTAAACGAGCATGCCGAAAGTGTAACTCCCTCTTTCTCTACGCCTTATAACGTGAATCG
CTTGATTGCGATTACGGCTTGTCCAACGGGAGTAACACAAACTTTTATGGCTGCCGAAGCCATTATGAACTATGCGAAAC
AACAAGGTTGGGATGTTAAGGTCGAAACCCGTGGACAGATTGGCGTGAAAGATATTCTAACCGAAGCGGATATTGCAGAG
GCTGATCTTGTTCTTGTGGCAACGGATGTGGAAACCGATCTGTCAAAATTTGAAGGAAAGATGTTATACCGTACATCAAC
ACGCGCAGTCCTCAACAATACAGAAAGCACTTTCCAACAAGCATTAACACAGGCGACAGTTTATCATGCGCCGAAAGAGG
ATGATGTCGGCATTATCACCCCACCACCACGTGACTACCACAATGATTTTGCGACCTGTGATGCCACTAAAAAGATTGTC
GCAGTTACTGCTTGTCCAACAGGGGTAACACAAACCTTTATGTCTGCAGAAGCGATCATGTTGTATGCGAAAACACAAGG
TTGGTGTGTCAAAGTCGAAACACACGGGCAAATGGGGGCAGATAACCTGATTACAGCTGAAGAAGTAGCAGCCGCTGACT
TAGTCTTTGCCGCCACGGATATAGAGATTGATTTATCGAAGTTTGAAGGTAAACCGCTTTATCGTACCTCAACGCATGCA
ACTTTAAAAAACATTGCACAAGAGTTTGATAAAGCATTCATGCAAGCGACTCCTTATACGCATACGAAGCAAGAAGCGTC
TAAAAATGCTTGTTGTTCAACAAGCACAACCAGCGAATGTTGTCAGTTAAAATGTGGTATAGCACGTTGGTTACCGCTCA
TTTTACTTGCCGCACTCATCATTGCGCTCGTAGCTTATTTTAAATAA

Upstream 100 bases:

>100_bases
AGCTTAGCAGTTTCACAAAGTAATGTAGGCATCACTGACTTGACGTTACTCAACCCAATTTTAGAAAAAATAAAAATCAC
ATTCATTTAAGGATAATTTC

Downstream 100 bases:

>100_bases
AACCTAGCCCGAATTGGCAATAAATATTCATAAGAAAAAAGCCCGACACATGCCGGGCTTTCTTGTTGATGAGGGGTCCC
TTAATTTTGACAATCTGCTT

Product: hypothetical protein

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNIFLTQKEDIGNAKVFLLHQVITAAAHQQGHQITTLDEADLVIVFGEAIHNPQFVGKKCVIVDAEEAFNAPENTLTKAF
AEATPYVLNEHAESVTPSFSTPYNVNRLIAITACPTGVTQTFMAAEAIMNYAKQQGWDVKVETRGQIGVKDILTEADIAE
ADLVLVATDVETDLSKFEGKMLYRTSTRAVLNNTESTFQQALTQATVYHAPKEDDVGIITPPPRDYHNDFATCDATKKIV
AVTACPTGVTQTFMSAEAIMLYAKTQGWCVKVETHGQMGADNLITAEEVAAADLVFAATDIEIDLSKFEGKPLYRTSTHA
TLKNIAQEFDKAFMQATPYTHTKQEASKNACCSTSTTSECCQLKCGIARWLPLILLAALIIALVAYFK

Sequences:

>Translated_388_residues
MNIFLTQKEDIGNAKVFLLHQVITAAAHQQGHQITTLDEADLVIVFGEAIHNPQFVGKKCVIVDAEEAFNAPENTLTKAF
AEATPYVLNEHAESVTPSFSTPYNVNRLIAITACPTGVTQTFMAAEAIMNYAKQQGWDVKVETRGQIGVKDILTEADIAE
ADLVLVATDVETDLSKFEGKMLYRTSTRAVLNNTESTFQQALTQATVYHAPKEDDVGIITPPPRDYHNDFATCDATKKIV
AVTACPTGVTQTFMSAEAIMLYAKTQGWCVKVETHGQMGADNLITAEEVAAADLVFAATDIEIDLSKFEGKPLYRTSTHA
TLKNIAQEFDKAFMQATPYTHTKQEASKNACCSTSTTSECCQLKCGIARWLPLILLAALIIALVAYFK
>Mature_388_residues
MNIFLTQKEDIGNAKVFLLHQVITAAAHQQGHQITTLDEADLVIVFGEAIHNPQFVGKKCVIVDAEEAFNAPENTLTKAF
AEATPYVLNEHAESVTPSFSTPYNVNRLIAITACPTGVTQTFMAAEAIMNYAKQQGWDVKVETRGQIGVKDILTEADIAE
ADLVLVATDVETDLSKFEGKMLYRTSTRAVLNNTESTFQQALTQATVYHAPKEDDVGIITPPPRDYHNDFATCDATKKIV
AVTACPTGVTQTFMSAEAIMLYAKTQGWCVKVETHGQMGADNLITAEEVAAADLVFAATDIEIDLSKFEGKPLYRTSTHA
TLKNIAQEFDKAFMQATPYTHTKQEASKNACCSTSTTSECCQLKCGIARWLPLILLAALIIALVAYFK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=151, Percent_Identity=54.3046357615894, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1790387, Length=88, Percent_Identity=48.8636363636364, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082348, Length=159, Percent_Identity=32.7044025157233, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788730, Length=89, Percent_Identity=38.2022471910112, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 42359; Mature: 42359

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFLTQKEDIGNAKVFLLHQVITAAAHQQGHQITTLDEADLVIVFGEAIHNPQFVGKKC
CCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCHHCCCCCCCCEE
VIVDAEEAFNAPENTLTKAFAEATPYVLNEHAESVTPSFSTPYNVNRLIAITACPTGVTQ
EEEECHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHH
TFMAAEAIMNYAKQQGWDVKVETRGQIGVKDILTEADIAEADLVLVATDVETDLSKFEGK
HHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCE
MLYRTSTRAVLNNTESTFQQALTQATVYHAPKEDDVGIITPPPRDYHNDFATCDATKKIV
EEEEECCHHHHCCCHHHHHHHHHHHHHEECCCCCCCEEECCCCCCCCCCCCCCCCCCEEE
AVTACPTGVTQTFMSAEAIMLYAKTQGWCVKVETHGQMGADNLITAEEVAAADLVFAATD
EEEECCCHHHHHHHCCEEEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHEEEEEEC
IEIDLSKFEGKPLYRTSTHATLKNIAQEFDKAFMQATPYTHTKQEASKNACCSTSTTSEC
EEEEHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHH
CQLKCGIARWLPLILLAALIIALVAYFK
HHHHHCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIFLTQKEDIGNAKVFLLHQVITAAAHQQGHQITTLDEADLVIVFGEAIHNPQFVGKKC
CCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCHHCCCCCCCCEE
VIVDAEEAFNAPENTLTKAFAEATPYVLNEHAESVTPSFSTPYNVNRLIAITACPTGVTQ
EEEECHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHH
TFMAAEAIMNYAKQQGWDVKVETRGQIGVKDILTEADIAEADLVLVATDVETDLSKFEGK
HHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCE
MLYRTSTRAVLNNTESTFQQALTQATVYHAPKEDDVGIITPPPRDYHNDFATCDATKKIV
EEEEECCHHHHCCCHHHHHHHHHHHHHEECCCCCCCEEECCCCCCCCCCCCCCCCCCEEE
AVTACPTGVTQTFMSAEAIMLYAKTQGWCVKVETHGQMGADNLITAEEVAAADLVFAATD
EEEECCCHHHHHHHCCEEEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHEEEEEEC
IEIDLSKFEGKPLYRTSTHATLKNIAQEFDKAFMQATPYTHTKQEASKNACCSTSTTSEC
EEEEHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHH
CQLKCGIARWLPLILLAALIIALVAYFK
HHHHHCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]