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The map label for this gene is iunH [H]

Identifier: 15603632

GI number: 15603632

Start: 1991928

End: 1992860

Strand: Direct

Name: iunH [H]

Synonym: PM1767

Alternate gene names: 15603632

Gene position: 1991928-1992860 (Clockwise)

Preceding gene: 15603630

Following gene: 15603634

Centisome position: 88.24

GC content: 41.69

Gene sequence:

>933_bases
ATGAAAAAAATTATTCTAGATTGTGATCCAGGGCATGATGATGCCATTGCGATTCTGTTAGCGCATGGCAATCCAGAAAT
TGATCTGTTAGCAATCACGACTGTGGTAGGTAATCAAACGCTAGAAAAAGTCAGTCGTAATGCTTTAGCAGTAGCTGAAA
TAGCCAATATCCTTAACGTACCTATTGCCAAAGGTTGTGTGCGACCTTTAGTGCGAGAAGTAGAAGTTGCACCAAGTATC
CATGGTGAATCGGGGATGGATGGACCTGTTTTGCCCGAACCCACACTGAGTTTAGATTCACGTCATGCCGTCGATTTAAT
TATTGAGTTAGTGATGACCCACCCGCCAAAAACAATTACGTTGGTGCCTACCGGTGGTTTAACGAATATTGCCATGGCGG
TGAGAAAAGAACCTCGTATTGTGGAACGCGTGAAGGAAATTGTCTTAATGGGAGGAGGCTATCACACAGGCAATTGGAGT
GCTGTTGCCGAATTTAATATTAAGATTGATCCTGAAGCTGCACATATTGTGTTCAATGAAAAATGGTCATTAACTATGGT
GGGATTGGATCTGACGCATCAAGCTTTAGCCACAGAAGATGTGATTAAAAACATTGAAAAAATTCAAACAAAACCAGCTA
AGTTTGTTCTCGAATTGTTGGCGTTTTTTCGCAAAATGTATAAGCAAGCACAAAATTTTGATTATCCGCCAGTACATGAT
CCTTGCGCTGTGGCTTATGTGATTGATCCGACGGTTTGTACGACGAAAGTGGTGCCCATTAATATTGAGTTAACAGGCAC
TCACACATTGGGGATGACAGTGGCAGATTTTCGTTATCCGATTCCTGAAAATTGTCACACACAGGTGGCAATGCAACTGG
ATAAAGCGAAATTTTGGGGCTTGATTTACGATGCTTTGCAACGTATTGGTTAA

Upstream 100 bases:

>100_bases
TTTTGAGCTTAAAGGATTGTCCTCAAACCCAATTAGCTCTACGCTGAAGGAAAATTATTAAGATAAACAAGATGTTATTC
TAAGTAGGAGAGGAATGGAC

Downstream 100 bases:

>100_bases
CGCATCAAGCAAGGGGCATATGCCCCTTTTGCTTTTTATAGCTGCATGATTGTCTTGAGGAGACTATCTATATCCGCTAA
ACCACGTAAATCTAACCAAA

Product: IunH

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKKIILDCDPGHDDAIAILLAHGNPEIDLLAITTVVGNQTLEKVSRNALAVAEIANILNVPIAKGCVRPLVREVEVAPSI
HGESGMDGPVLPEPTLSLDSRHAVDLIIELVMTHPPKTITLVPTGGLTNIAMAVRKEPRIVERVKEIVLMGGGYHTGNWS
AVAEFNIKIDPEAAHIVFNEKWSLTMVGLDLTHQALATEDVIKNIEKIQTKPAKFVLELLAFFRKMYKQAQNFDYPPVHD
PCAVAYVIDPTVCTTKVVPINIELTGTHTLGMTVADFRYPIPENCHTQVAMQLDKAKFWGLIYDALQRIG

Sequences:

>Translated_310_residues
MKKIILDCDPGHDDAIAILLAHGNPEIDLLAITTVVGNQTLEKVSRNALAVAEIANILNVPIAKGCVRPLVREVEVAPSI
HGESGMDGPVLPEPTLSLDSRHAVDLIIELVMTHPPKTITLVPTGGLTNIAMAVRKEPRIVERVKEIVLMGGGYHTGNWS
AVAEFNIKIDPEAAHIVFNEKWSLTMVGLDLTHQALATEDVIKNIEKIQTKPAKFVLELLAFFRKMYKQAQNFDYPPVHD
PCAVAYVIDPTVCTTKVVPINIELTGTHTLGMTVADFRYPIPENCHTQVAMQLDKAKFWGLIYDALQRIG
>Mature_310_residues
MKKIILDCDPGHDDAIAILLAHGNPEIDLLAITTVVGNQTLEKVSRNALAVAEIANILNVPIAKGCVRPLVREVEVAPSI
HGESGMDGPVLPEPTLSLDSRHAVDLIIELVMTHPPKTITLVPTGGLTNIAMAVRKEPRIVERVKEIVLMGGGYHTGNWS
AVAEFNIKIDPEAAHIVFNEKWSLTMVGLDLTHQALATEDVIKNIEKIQTKPAKFVLELLAFFRKMYKQAQNFDYPPVHD
PCAVAYVIDPTVCTTKVVPINIELTGTHTLGMTVADFRYPIPENCHTQVAMQLDKAKFWGLIYDALQRIG

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=306, Percent_Identity=42.8104575163399, Blast_Score=240, Evalue=8e-65,
Organism=Escherichia coli, GI1786871, Length=305, Percent_Identity=40.9836065573771, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1786213, Length=303, Percent_Identity=36.963696369637, Blast_Score=161, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI32565474, Length=268, Percent_Identity=33.955223880597, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17565698, Length=299, Percent_Identity=27.4247491638796, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320608, Length=325, Percent_Identity=37.5384615384615, Blast_Score=180, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24583915, Length=189, Percent_Identity=32.8042328042328, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24641837, Length=191, Percent_Identity=36.6492146596859, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24641839, Length=309, Percent_Identity=26.537216828479, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 34040; Mature: 34040

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIILDCDPGHDDAIAILLAHGNPEIDLLAITTVVGNQTLEKVSRNALAVAEIANILNV
CCEEEEECCCCCCCEEEEEEECCCCCEEEEEEEEHHCCHHHHHHHHCHHHHHHHHHHHCC
PIAKGCVRPLVREVEVAPSIHGESGMDGPVLPEPTLSLDSRHAVDLIIELVMTHPPKTIT
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
LVPTGGLTNIAMAVRKEPRIVERVKEIVLMGGGYHTGNWSAVAEFNIKIDPEAAHIVFNE
EEECCCHHHHHHHHCCCCHHHHHHHHHEEECCCEECCCCEEEEEEEEEECCCEEEEEEEC
KWSLTMVGLDLTHQALATEDVIKNIEKIQTKPAKFVLELLAFFRKMYKQAQNFDYPPVHD
CEEEEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PCAVAYVIDPTVCTTKVVPINIELTGTHTLGMTVADFRYPIPENCHTQVAMQLDKAKFWG
CEEEEEEECCCCCEEEEEEEEEEEECCEEECCEEECCCCCCCCCCHHHHHHHHHHHHHHH
LIYDALQRIG
HHHHHHHHCC
>Mature Secondary Structure
MKKIILDCDPGHDDAIAILLAHGNPEIDLLAITTVVGNQTLEKVSRNALAVAEIANILNV
CCEEEEECCCCCCCEEEEEEECCCCCEEEEEEEEHHCCHHHHHHHHCHHHHHHHHHHHCC
PIAKGCVRPLVREVEVAPSIHGESGMDGPVLPEPTLSLDSRHAVDLIIELVMTHPPKTIT
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
LVPTGGLTNIAMAVRKEPRIVERVKEIVLMGGGYHTGNWSAVAEFNIKIDPEAAHIVFNE
EEECCCHHHHHHHHCCCCHHHHHHHHHEEECCCEECCCCEEEEEEEEEECCCEEEEEEEC
KWSLTMVGLDLTHQALATEDVIKNIEKIQTKPAKFVLELLAFFRKMYKQAQNFDYPPVHD
CEEEEEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PCAVAYVIDPTVCTTKVVPINIELTGTHTLGMTVADFRYPIPENCHTQVAMQLDKAKFWG
CEEEEEEECCCCCEEEEEEEEEEEECCEEECCEEECCCCCCCCCCHHHHHHHHHHHHHHH
LIYDALQRIG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA