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The map label for this gene is napF [C]

Identifier: 15603623

GI number: 15603623

Start: 1981681

End: 1982226

Strand: Direct

Name: napF [C]

Synonym: PM1758

Alternate gene names: 15603623

Gene position: 1981681-1982226 (Clockwise)

Preceding gene: 15603622

Following gene: 15603629

Centisome position: 87.78

GC content: 42.67

Gene sequence:

>546_bases
ATGCACAATAATGACTCAACTCATCGGCACCGTCAGAAAGATGAACGTTATTATGAAGCCTATCTTTCTCACCATACTAT
TTCACGTCGTGGTTTATTGCGTGGCTTATTGGGGGGAAACACGAAACAGGAAAAACGTTTAAAAAATCGACCGCCCTTTG
CGGCTAAAGAAGCCTTATTACTTCAAGTGTGTCATGGATGTGCGGATTGTATTGATGCCTGTCCTTATGGCTTGCTTCGC
TTGGTGGAAGGAAAACCCGTGCTAGAAATTGATTTTTCAGCCTGTGATTTTTGTGGTAAATGTGCCGAAGCTTGTACTAC
AGGCGCCTTACATCGAGCATTTTCAGCGGACACGGAATTACGTCCAATCTTTAGCGAAAAATGTTTAATTACACAAGGGC
AATCCTGTTCTGAATGTCAGCAAAAATGCCCACAACATGCCTTGTCGATTCAACATAAACAACTTGTTGTTAGTCCAGCT
TGTCATGGCTGTGGTCAATGCAAGATAAGTTGTATGCTGGGGGCAATTACCTTATCTCGCTGTTAA

Upstream 100 bases:

>100_bases
TTGCGTTATTGACGCAAGCAACGTTAAATCAATGGCAGCAACAGCTTGATATTCAAGTGAACAGCGTACGTCTTTATCGT
TAAATCACATGAGGCAAAGC

Downstream 100 bases:

>100_bases
AACATCTTTATTCTTCTTAAGTGGGAAGCCAGAAAAAAGGGGCGTATAACGCCCCAACTAACCTAATTGTGCTTTTAAGA
CAGCACATGATTCTTGATTT

Product: hypothetical protein

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MHNNDSTHRHRQKDERYYEAYLSHHTISRRGLLRGLLGGNTKQEKRLKNRPPFAAKEALLLQVCHGCADCIDACPYGLLR
LVEGKPVLEIDFSACDFCGKCAEACTTGALHRAFSADTELRPIFSEKCLITQGQSCSECQQKCPQHALSIQHKQLVVSPA
CHGCGQCKISCMLGAITLSRC

Sequences:

>Translated_181_residues
MHNNDSTHRHRQKDERYYEAYLSHHTISRRGLLRGLLGGNTKQEKRLKNRPPFAAKEALLLQVCHGCADCIDACPYGLLR
LVEGKPVLEIDFSACDFCGKCAEACTTGALHRAFSADTELRPIFSEKCLITQGQSCSECQQKCPQHALSIQHKQLVVSPA
CHGCGQCKISCMLGAITLSRC
>Mature_181_residues
MHNNDSTHRHRQKDERYYEAYLSHHTISRRGLLRGLLGGNTKQEKRLKNRPPFAAKEALLLQVCHGCADCIDACPYGLLR
LVEGKPVLEIDFSACDFCGKCAEACTTGALHRAFSADTELRPIFSEKCLITQGQSCSECQQKCPQHALSIQHKQLVVSPA
CHGCGQCKISCMLGAITLSRC

Specific function: Involved In Electron Transfer. [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=159, Percent_Identity=32.0754716981132, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788533, Length=161, Percent_Identity=29.1925465838509, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 20046; Mature: 20046

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
10.5 %Cys+Met (Translated Protein)
9.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
10.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNNDSTHRHRQKDERYYEAYLSHHTISRRGLLRGLLGGNTKQEKRLKNRPPFAAKEALL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHH
LQVCHGCADCIDACPYGLLRLVEGKPVLEIDFSACDFCGKCAEACTTGALHRAFSADTEL
HHHHHHHHHHHHHCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RPIFSEKCLITQGQSCSECQQKCPQHALSIQHKQLVVSPACHGCGQCKISCMLGAITLSR
CHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCC
C
C
>Mature Secondary Structure
MHNNDSTHRHRQKDERYYEAYLSHHTISRRGLLRGLLGGNTKQEKRLKNRPPFAAKEALL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHH
LQVCHGCADCIDACPYGLLRLVEGKPVLEIDFSACDFCGKCAEACTTGALHRAFSADTEL
HHHHHHHHHHHHHCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RPIFSEKCLITQGQSCSECQQKCPQHALSIQHKQLVVSPACHGCGQCKISCMLGAITLSR
CHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCC
C
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]