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The map label for this gene is ilvA

Identifier: 15603489

GI number: 15603489

Start: 1833711

End: 1835252

Strand: Reverse

Name: ilvA

Synonym: PM1624

Alternate gene names: 15603489

Gene position: 1835252-1833711 (Counterclockwise)

Preceding gene: 15603490

Following gene: 15603483

Centisome position: 81.3

GC content: 43.77

Gene sequence:

>1542_bases
ATGGTCAATAACCTCCATTCAGCTCACCCAACAGGTGCAGAATACCTCAAAGCCGTGTTGAGTTCTAAAGTCTATGACGT
GGCGCAAGTGACTCCGCTTCAAGACATGGCAAAACTGTCTGAACGTTTAGGTAATAAAGTGTTTATCAAACGTGAAGATC
GCCAACCCGTACACAGTTTCAAACTGCGTGGTGCGTATGCCATGATTGCTGGCTTAAGTGCCGAACAAAAAGCCTCGGGC
GTGATTGCCGCCTCAGCGGGAAACCATGCACAAGGCGTGGCGCTGTCTGCCAAACATCTTGGCTTGCGTGCCTTAATCGT
GATGCCACAAAACACCCCAAGTATCAAAGTCGATGCCGTGCGTGGATTTGGTGGTGAAGTGCTATTACATGGCGCCAATT
TCGATGAAGCCAAAGCCAAAGCGATTGAATTGGCGGAAAGCAAAAATATGACATTCATTCCGCCTTTTGATCACCCCGCG
GTGATTGCAGGACAAGGCTCGATTGCCATGGAATTGTTACAACAAAACAGCCAAATTGATCGTATTTTTGTCCCTGTTGG
TGGCGGTGGCTTAGCCGCAGGCATTGCTGTATTAATTAAACAATTAATGCCCGAAATCAAAGTAATTGGGGTTGAGTCGA
AAGATTCCGCCTGCTTGTATCGTGCTCTCAAAGCAGGTAAGCCCATTGATTTAGATCGCGTTGGCTTATTTGCTGACGGG
GTCGCAGTGAAACGTATTGGCGATGAGACTTTCCGCGTTTGTCAGCAATATATTGATGATGTAGTGTTAGTAGATGGTGA
TGAAATCTGCGCTGCGGTCAAAGATATTTTTGAGAATGTGCGCGCTATTGCTGAACCGTCGGGGGCATTATCGCTTGCTG
GTTTAAAAAAATACGTGAAAGAGCACAATATTCAAGGTGAAACCTTGGTGAATGTATTATCTGGTGCCAATTTAAATTTC
CATACCTTACGTTATGTGTCAGAGCGTTGTGAAATTGGTGAACAGCACGAAGCCCTTTTAGCCGTGACCATTCCTGAACA
ACCAGGGAGTTTCTTAAAATTCTGCCATATTTTAGGTCATGTCCCTGTTACTGAATTTAAATACCGTTATGCCGACGACA
AACAAGCTTGTATTTTCGTGGGGGTACGCATTACAGGGCAAGAAGAAAAACAAACGATCATCAATCAATTACAACAAAAT
GGTTATGATTTAATTGATTTGTCTAATGATGATATCGCCAAAACGCATGTACGCTATATGATTGGCGGACGTTCAAACAG
TCCCTTAAAAGAGCGTTTATACAGTTTTGAATTCCCAGAACAAAAAGGCGCCTTATTAAAATTCCTCGAAACTTTGGGGC
AAACACATTGGAATATTTCCGTGTTCCATTATCGTGCGCACGGGGCAGATTACGGTAACGTGCTTGCTGGCTTCCAATTA
AATGATGAAGATCTCGACGCCTTTAATCAGCATTTGGAAAAACTGGGTTATGTCTATCAAGATGTCACAGAAAGCCCTGC
TTATCGTTATTTCTTAGTGTAA

Upstream 100 bases:

>100_bases
TCGGGTGGGTAAGCTTGCCCACCGTTTTCTCATTGATAAATTGATTACGATTTTTTTCTTGCTGAAACAAGAAAATTTAA
AAAAATAACGGACAACCTCT

Downstream 100 bases:

>100_bases
GTCAAGAAAAAAGTGCGGTTATTTTGACCGCACTTTTTAATACTCTTTGCAACCTTTGTTACATGTGTTATTTCACAAAC
CCACTAAACTCTAATTCGTA

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MVNNLHSAHPTGAEYLKAVLSSKVYDVAQVTPLQDMAKLSERLGNKVFIKREDRQPVHSFKLRGAYAMIAGLSAEQKASG
VIAASAGNHAQGVALSAKHLGLRALIVMPQNTPSIKVDAVRGFGGEVLLHGANFDEAKAKAIELAESKNMTFIPPFDHPA
VIAGQGSIAMELLQQNSQIDRIFVPVGGGGLAAGIAVLIKQLMPEIKVIGVESKDSACLYRALKAGKPIDLDRVGLFADG
VAVKRIGDETFRVCQQYIDDVVLVDGDEICAAVKDIFENVRAIAEPSGALSLAGLKKYVKEHNIQGETLVNVLSGANLNF
HTLRYVSERCEIGEQHEALLAVTIPEQPGSFLKFCHILGHVPVTEFKYRYADDKQACIFVGVRITGQEEKQTIINQLQQN
GYDLIDLSNDDIAKTHVRYMIGGRSNSPLKERLYSFEFPEQKGALLKFLETLGQTHWNISVFHYRAHGADYGNVLAGFQL
NDEDLDAFNQHLEKLGYVYQDVTESPAYRYFLV

Sequences:

>Translated_513_residues
MVNNLHSAHPTGAEYLKAVLSSKVYDVAQVTPLQDMAKLSERLGNKVFIKREDRQPVHSFKLRGAYAMIAGLSAEQKASG
VIAASAGNHAQGVALSAKHLGLRALIVMPQNTPSIKVDAVRGFGGEVLLHGANFDEAKAKAIELAESKNMTFIPPFDHPA
VIAGQGSIAMELLQQNSQIDRIFVPVGGGGLAAGIAVLIKQLMPEIKVIGVESKDSACLYRALKAGKPIDLDRVGLFADG
VAVKRIGDETFRVCQQYIDDVVLVDGDEICAAVKDIFENVRAIAEPSGALSLAGLKKYVKEHNIQGETLVNVLSGANLNF
HTLRYVSERCEIGEQHEALLAVTIPEQPGSFLKFCHILGHVPVTEFKYRYADDKQACIFVGVRITGQEEKQTIINQLQQN
GYDLIDLSNDDIAKTHVRYMIGGRSNSPLKERLYSFEFPEQKGALLKFLETLGQTHWNISVFHYRAHGADYGNVLAGFQL
NDEDLDAFNQHLEKLGYVYQDVTESPAYRYFLV
>Mature_513_residues
MVNNLHSAHPTGAEYLKAVLSSKVYDVAQVTPLQDMAKLSERLGNKVFIKREDRQPVHSFKLRGAYAMIAGLSAEQKASG
VIAASAGNHAQGVALSAKHLGLRALIVMPQNTPSIKVDAVRGFGGEVLLHGANFDEAKAKAIELAESKNMTFIPPFDHPA
VIAGQGSIAMELLQQNSQIDRIFVPVGGGGLAAGIAVLIKQLMPEIKVIGVESKDSACLYRALKAGKPIDLDRVGLFADG
VAVKRIGDETFRVCQQYIDDVVLVDGDEICAAVKDIFENVRAIAEPSGALSLAGLKKYVKEHNIQGETLVNVLSGANLNF
HTLRYVSERCEIGEQHEALLAVTIPEQPGSFLKFCHILGHVPVTEFKYRYADDKQACIFVGVRITGQEEKQTIINQLQQN
GYDLIDLSNDDIAKTHVRYMIGGRSNSPLKERLYSFEFPEQKGALLKFLETLGQTHWNISVFHYRAHGADYGNVLAGFQL
NDEDLDAFNQHLEKLGYVYQDVTESPAYRYFLV

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family

Homologues:

Organism=Homo sapiens, GI11345492, Length=304, Percent_Identity=32.2368421052632, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI19923959, Length=308, Percent_Identity=29.8701298701299, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI33469958, Length=313, Percent_Identity=26.517571884984, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1790207, Length=507, Percent_Identity=69.8224852071006, Blast_Score=717, Evalue=0.0,
Organism=Escherichia coli, GI1789505, Length=289, Percent_Identity=39.4463667820069, Blast_Score=191, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI71991565, Length=346, Percent_Identity=32.0809248554913, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17537387, Length=381, Percent_Identity=31.758530183727, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17508781, Length=285, Percent_Identity=34.0350877192982, Blast_Score=141, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6320930, Length=515, Percent_Identity=50.4854368932039, Blast_Score=480, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6322631, Length=318, Percent_Identity=34.9056603773585, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319788, Length=265, Percent_Identity=26.4150943396226, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355833, Length=325, Percent_Identity=31.6923076923077, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24645328, Length=257, Percent_Identity=29.9610894941634, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): THD1_PASMU (Q9CKJ2)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246563.1
- ProteinModelPortal:   Q9CKJ2
- SMR:   Q9CKJ2
- PRIDE:   Q9CKJ2
- GeneID:   1244971
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1624
- NMPDR:   fig|272843.1.peg.1625
- HOGENOM:   HBG714501
- OMA:   YIAPFDD
- ProtClustDB:   PRK09224
- BioCyc:   PMUL272843:PM1624-MONOMER
- BRENDA:   4.3.1.19
- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721
- TIGRFAMs:   TIGR01124

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.3.1.19

Molecular weight: Translated: 56289; Mature: 56289

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNNLHSAHPTGAEYLKAVLSSKVYDVAQVTPLQDMAKLSERLGNKVFIKREDRQPVHSF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCHHHH
KLRGAYAMIAGLSAEQKASGVIAASAGNHAQGVALSAKHLGLRALIVMPQNTPSIKVDAV
HHCCHHHEEECCCCCHHCCCEEEECCCCCCCCEEEEHHHCCEEEEEEECCCCCCEEEEEE
RGFGGEVLLHGANFDEAKAKAIELAESKNMTFIPPFDHPAVIAGQGSIAMELLQQNSQID
ECCCCEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEECCCHHHHHHHHCCCCCC
RIFVPVGGGGLAAGIAVLIKQLMPEIKVIGVESKDSACLYRALKAGKPIDLDRVGLFADG
EEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCHHCCCEEECC
VAVKRIGDETFRVCQQYIDDVVLVDGDEICAAVKDIFENVRAIAEPSGALSLAGLKKYVK
CHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
EHNIQGETLVNVLSGANLNFHTLRYVSERCEIGEQHEALLAVTIPEQPGSFLKFCHILGH
HCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCC
VPVTEFKYRYADDKQACIFVGVRITGQEEKQTIINQLQQNGYDLIDLSNDDIAKTHVRYM
CCHHHEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCHHHHEEEEE
IGGRSNSPLKERLYSFEFPEQKGALLKFLETLGQTHWNISVFHYRAHGADYGNVLAGFQL
ECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEECCEE
NDEDLDAFNQHLEKLGYVYQDVTESPAYRYFLV
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MVNNLHSAHPTGAEYLKAVLSSKVYDVAQVTPLQDMAKLSERLGNKVFIKREDRQPVHSF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCHHHH
KLRGAYAMIAGLSAEQKASGVIAASAGNHAQGVALSAKHLGLRALIVMPQNTPSIKVDAV
HHCCHHHEEECCCCCHHCCCEEEECCCCCCCCEEEEHHHCCEEEEEEECCCCCCEEEEEE
RGFGGEVLLHGANFDEAKAKAIELAESKNMTFIPPFDHPAVIAGQGSIAMELLQQNSQID
ECCCCEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEECCCHHHHHHHHCCCCCC
RIFVPVGGGGLAAGIAVLIKQLMPEIKVIGVESKDSACLYRALKAGKPIDLDRVGLFADG
EEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCHHCCCEEECC
VAVKRIGDETFRVCQQYIDDVVLVDGDEICAAVKDIFENVRAIAEPSGALSLAGLKKYVK
CHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
EHNIQGETLVNVLSGANLNFHTLRYVSERCEIGEQHEALLAVTIPEQPGSFLKFCHILGH
HCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCC
VPVTEFKYRYADDKQACIFVGVRITGQEEKQTIINQLQQNGYDLIDLSNDDIAKTHVRYM
CCHHHEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCHHHHEEEEE
IGGRSNSPLKERLYSFEFPEQKGALLKFLETLGQTHWNISVFHYRAHGADYGNVLAGFQL
ECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEECCEE
NDEDLDAFNQHLEKLGYVYQDVTESPAYRYFLV
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100