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The map label for this gene is rluE

Identifier: 15603470

GI number: 15603470

Start: 1816565

End: 1817290

Strand: Reverse

Name: rluE

Synonym: PM1605

Alternate gene names: 15603470

Gene position: 1817290-1816565 (Counterclockwise)

Preceding gene: 15603480

Following gene: 15603469

Centisome position: 80.5

GC content: 42.01

Gene sequence:

>726_bases
ATGAAACCAAACACCAAATTTAACCAAAAAACGAAACCACGTCCTTCGTCACTACGATCCGTGAATAAAAGAAAAGCAGC
AGAAATTAAACCACAAAAGCGTCCTTTATCCTTTCGTCAGAAAAAAACAGTGAATTTTGCCGAAACCCAAGTCATTTTGT
TTAACAAGCCTTATGATGTGTTGACACAATTTAGCGATGAGGAAGGACGTGCCACCTTAAAAGCGTTTATCCCGATTCCT
CATGTCTATCCCGCTGGACGTTTAGATCGCGATAGTGAAGGCTTAGTGTTACTGACCAATAACGGCGAAATCCAACATCG
TTTGGCAGTACCCAAATTCAACACAGAAAAAACCTATTTCGCCCAAGTGGAAGGCATTCCGCAAGAAACGGATTTAGCAC
AACTGCGTCAAGGCGTGGTCTTAAACGATGGTAAAACATTACCCGCCAAAGTACGCTTAGTGCCTGAGCCAAACTGGTTG
TGGCAACGCAATCCACCGATCCGTGAACGCAAACAGATTCCGACCAGTTGGTTGGAAATTAAAATACATGAAGGGCGTAA
CCGCCAAGTGCGCCGCATGACGGCACATATTGGCTTTCCTACTTTGCGTTTAATTCGTTATCAAATGGCATTGTTTAATT
TAGCCAATCTTGAACAAGGGCAATATCGCCAACTTTCTGAAGTAGAATTGACCGCACTTTATCAACAATTAAAGTTAAAA
AAATAA

Upstream 100 bases:

>100_bases
TTGGAACTGGCGCAAGTTCGCAAGCATTTATTAAATTTTTGTTAAAAAATTTCTTTATTTTTCGATCTAGATCCCATTTT
CAACTGACCACCTTTAACAT

Downstream 100 bases:

>100_bases
TAAGGACAGGCTATGCATAAACCCAACGTCACCCTTGCCTGTGTGGTGCATTGCAAAGGCAAATTTTTATTTGTAGAAGA
ACTGGAACACGGCAAACTGA

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKPNTKFNQKTKPRPSSLRSVNKRKAAEIKPQKRPLSFRQKKTVNFAETQVILFNKPYDVLTQFSDEEGRATLKAFIPIP
HVYPAGRLDRDSEGLVLLTNNGEIQHRLAVPKFNTEKTYFAQVEGIPQETDLAQLRQGVVLNDGKTLPAKVRLVPEPNWL
WQRNPPIRERKQIPTSWLEIKIHEGRNRQVRRMTAHIGFPTLRLIRYQMALFNLANLEQGQYRQLSEVELTALYQQLKLK
K

Sequences:

>Translated_241_residues
MKPNTKFNQKTKPRPSSLRSVNKRKAAEIKPQKRPLSFRQKKTVNFAETQVILFNKPYDVLTQFSDEEGRATLKAFIPIP
HVYPAGRLDRDSEGLVLLTNNGEIQHRLAVPKFNTEKTYFAQVEGIPQETDLAQLRQGVVLNDGKTLPAKVRLVPEPNWL
WQRNPPIRERKQIPTSWLEIKIHEGRNRQVRRMTAHIGFPTLRLIRYQMALFNLANLEQGQYRQLSEVELTALYQQLKLK
K
>Mature_241_residues
MKPNTKFNQKTKPRPSSLRSVNKRKAAEIKPQKRPLSFRQKKTVNFAETQVILFNKPYDVLTQFSDEEGRATLKAFIPIP
HVYPAGRLDRDSEGLVLLTNNGEIQHRLAVPKFNTEKTYFAQVEGIPQETDLAQLRQGVVLNDGKTLPAKVRLVPEPNWL
WQRNPPIRERKQIPTSWLEIKIHEGRNRQVRRMTAHIGFPTLRLIRYQMALFNLANLEQGQYRQLSEVELTALYQQLKLK
K

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family

Homologues:

Organism=Escherichia coli, GI87081838, Length=219, Percent_Identity=57.9908675799087, Blast_Score=257, Evalue=6e-70,
Organism=Escherichia coli, GI1788510, Length=187, Percent_Identity=31.0160427807487, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790453, Length=189, Percent_Identity=26.984126984127, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787524, Length=197, Percent_Identity=29.4416243654822, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUE_PASMU (Q9CKK7)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246544.1
- ProteinModelPortal:   Q9CKK7
- SMR:   Q9CKK7
- GeneID:   1244952
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1605
- NMPDR:   fig|272843.1.peg.1606
- HOGENOM:   HBG754478
- OMA:   KHKLAKT
- BioCyc:   PMUL272843:PM1605-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 28058; Mature: 28058

Theoretical pI: Translated: 11.01; Mature: 11.01

Prosite motif: PS01149 PSI_RSU

Important sites: ACT_SITE 89-89

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNTKFNQKTKPRPSSLRSVNKRKAAEIKPQKRPLSFRQKKTVNFAETQVILFNKPYDV
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCEEEEEEECCHHH
LTQFSDEEGRATLKAFIPIPHVYPAGRLDRDSEGLVLLTNNGEIQHRLAVPKFNTEKTYF
HHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEE
AQVEGIPQETDLAQLRQGVVLNDGKTLPAKVRLVPEPNWLWQRNPPIRERKQIPTSWLEI
EEECCCCCCCCHHHHHCCEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCEEEEE
KIHEGRNRQVRRMTAHIGFPTLRLIRYQMALFNLANLEQGQYRQLSEVELTALYQQLKLK
EEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
K
C
>Mature Secondary Structure
MKPNTKFNQKTKPRPSSLRSVNKRKAAEIKPQKRPLSFRQKKTVNFAETQVILFNKPYDV
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCEEEEEEECCHHH
LTQFSDEEGRATLKAFIPIPHVYPAGRLDRDSEGLVLLTNNGEIQHRLAVPKFNTEKTYF
HHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEE
AQVEGIPQETDLAQLRQGVVLNDGKTLPAKVRLVPEPNWLWQRNPPIRERKQIPTSWLEI
EEECCCCCCCCHHHHHCCEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCEEEEE
KIHEGRNRQVRRMTAHIGFPTLRLIRYQMALFNLANLEQGQYRQLSEVELTALYQQLKLK
EEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100