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The map label for this gene is rimO

Identifier: 15603436

GI number: 15603436

Start: 1779706

End: 1781046

Strand: Reverse

Name: rimO

Synonym: PM1571

Alternate gene names: 15603436

Gene position: 1781046-1779706 (Counterclockwise)

Preceding gene: 15603437

Following gene: 15603435

Centisome position: 78.9

GC content: 41.39

Gene sequence:

>1341_bases
ATGACATCTTCTTTACCCAATATTGGTTTTATTAGTCTCGGTTGCCCTAAAAATTTAGTAGATTCTGAACGTATTCTGAC
GGAGTTACGTTCAGATGGTTACAATATTATTCCAAGTTATGAAAATGCGGATCTTGTCATTGTCAACACTTGTGGTTTTA
TTGACAGCGCAGTACAAGAATCCCTCGAGGCAATTGGCGAAGCGCTGGAAGAAAATGGCAAAGTGATTGTGACGGGTTGC
TTAGGTGCCAAAGAAGATCGTATTCGCGAAGTCCACCCGAAAGTGTTAGAAGTAACAGGTCCACATAGTTACGAAGCTGT
CATGCAGCAAGTGCATAAATATGTACCTAAGCCTGCCTATAATCCGTATGTCAATCTTGTACCCAAACAAGGTGTAAAAC
TGACACCAAAACATTATGCCTATTTAAAAATCTCAGAAGGCTGTGATCATCGTTGTACTTTCTGCATTATTCCTTCCATG
CGTGGTGACTTAGACAGTCGTTCCATTACACAAGTATTAGATGAAGCAAAACGCCTTGTTGAAGCGGGAGTAAAAGAAAT
CTTGGTCGTTTCACAAGATACTTCTGCCTATGCGTTAGATCGTAGCAAAGAAGAACAAAATAAAACGGTATTCTGGAATG
GCATGCCAATTAAAAATAACTTAATTAGCCTCTGTGAGCAACTGGGTAAACTCGGTGTGTGGGTACGCTTACATTATGTT
TACCCTTACCCACATGTTGATCAATTAATTCCATTAATGGCGGAAGGCAAAATCCTGCCTTATTTAGATATTCCCTTACA
ACATGCTAGCCCTAAAATCTTAAAAGCCATGAAAAGACCCGGTTCAATTGAGCGAACCCTAGAACGGATCAAAAAATGGC
GTGAAATCTGTCCTGATCTCACATTACGCTCAACCTTTATTGTCGGATTTCCGGGTGAAAGCGAAGAAGATTTTCAACTT
CTTTTAGATTTCTTAAAAGAAGCCCTACTTGATCGCGTTGGCTGTTTCAAATTCAGCCCAGTAGAAGGAGCTATTGCAAC
AGAAATGCCCGATCAAGTCCCAGAAGAGGTGAAAGAAGCACGTTTTCACCGCTTTATGCAGTTACAACAAGAAATCTCTG
CAGCGCGCCTACAACAAAAAGTAGGCAAGGTCTTCACTGTGCTCGTGGACGAAGTAGATGAAGAAGGCATCATTGCACGT
TCCATAGCAGATGCACCAGAAATAGATGGTGTGGTTTACATTGATAATCCAAATCGAGTGGCTGTTAAAGCGGGACAATT
TATTGAAGTCAAGATCACACGGGCTGACGCCTACGATCTTTACGCGTCGCTAATAAACTAG

Upstream 100 bases:

>100_bases
GCTTTTTTTAGACTTTTTCTGCTCATTTCGTAGTCTTCTCTAGATAAATTCTGTACAATAAAGAATTACTCGAATTTATT
CCTTTTTTGAGATCTTTTTT

Downstream 100 bases:

>100_bases
TTATTTCTTTTAATAACTTTACATTATAAGCATAGTGTTCACTTCGCCATCGTCGTAGAATTAGCAACCAATTTTTTTAC
GTCCTTGTTTCATAGCAGTA

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTSSLPNIGFISLGCPKNLVDSERILTELRSDGYNIIPSYENADLVIVNTCGFIDSAVQESLEAIGEALEENGKVIVTGC
LGAKEDRIREVHPKVLEVTGPHSYEAVMQQVHKYVPKPAYNPYVNLVPKQGVKLTPKHYAYLKISEGCDHRCTFCIIPSM
RGDLDSRSITQVLDEAKRLVEAGVKEILVVSQDTSAYALDRSKEEQNKTVFWNGMPIKNNLISLCEQLGKLGVWVRLHYV
YPYPHVDQLIPLMAEGKILPYLDIPLQHASPKILKAMKRPGSIERTLERIKKWREICPDLTLRSTFIVGFPGESEEDFQL
LLDFLKEALLDRVGCFKFSPVEGAIATEMPDQVPEEVKEARFHRFMQLQQEISAARLQQKVGKVFTVLVDEVDEEGIIAR
SIADAPEIDGVVYIDNPNRVAVKAGQFIEVKITRADAYDLYASLIN

Sequences:

>Translated_446_residues
MTSSLPNIGFISLGCPKNLVDSERILTELRSDGYNIIPSYENADLVIVNTCGFIDSAVQESLEAIGEALEENGKVIVTGC
LGAKEDRIREVHPKVLEVTGPHSYEAVMQQVHKYVPKPAYNPYVNLVPKQGVKLTPKHYAYLKISEGCDHRCTFCIIPSM
RGDLDSRSITQVLDEAKRLVEAGVKEILVVSQDTSAYALDRSKEEQNKTVFWNGMPIKNNLISLCEQLGKLGVWVRLHYV
YPYPHVDQLIPLMAEGKILPYLDIPLQHASPKILKAMKRPGSIERTLERIKKWREICPDLTLRSTFIVGFPGESEEDFQL
LLDFLKEALLDRVGCFKFSPVEGAIATEMPDQVPEEVKEARFHRFMQLQQEISAARLQQKVGKVFTVLVDEVDEEGIIAR
SIADAPEIDGVVYIDNPNRVAVKAGQFIEVKITRADAYDLYASLIN
>Mature_445_residues
TSSLPNIGFISLGCPKNLVDSERILTELRSDGYNIIPSYENADLVIVNTCGFIDSAVQESLEAIGEALEENGKVIVTGCL
GAKEDRIREVHPKVLEVTGPHSYEAVMQQVHKYVPKPAYNPYVNLVPKQGVKLTPKHYAYLKISEGCDHRCTFCIIPSMR
GDLDSRSITQVLDEAKRLVEAGVKEILVVSQDTSAYALDRSKEEQNKTVFWNGMPIKNNLISLCEQLGKLGVWVRLHYVY
PYPHVDQLIPLMAEGKILPYLDIPLQHASPKILKAMKRPGSIERTLERIKKWREICPDLTLRSTFIVGFPGESEEDFQLL
LDFLKEALLDRVGCFKFSPVEGAIATEMPDQVPEEVKEARFHRFMQLQQEISAARLQQKVGKVFTVLVDEVDEEGIIARS
IADAPEIDGVVYIDNPNRVAVKAGQFIEVKITRADAYDLYASLIN

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=27.6150627615063, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=27.6150627615063, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI93277076, Length=423, Percent_Identity=26.4775413711584, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1787057, Length=440, Percent_Identity=75.2272727272727, Blast_Score=704, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=443, Percent_Identity=25.7336343115124, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17553146, Length=480, Percent_Identity=26.875, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71996771, Length=314, Percent_Identity=24.5222929936306, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=25.9958071278826, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19922432, Length=468, Percent_Identity=23.7179487179487, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_PASMU (Q9CKN9)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246510.1
- ProteinModelPortal:   Q9CKN9
- SMR:   Q9CKN9
- GeneID:   1244918
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1571
- NMPDR:   fig|272843.1.peg.1572
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   PRK14862
- BioCyc:   PMUL272843:PM1571-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50071; Mature: 49940

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSLPNIGFISLGCPKNLVDSERILTELRSDGYNIIPSYENADLVIVNTCGFIDSAVQE
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEEEECCCHHHHHHHH
SLEAIGEALEENGKVIVTGCLGAKEDRIREVHPKVLEVTGPHSYEAVMQQVHKYVPKPAY
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCC
NPYVNLVPKQGVKLTPKHYAYLKISEGCDHRCTFCIIPSMRGDLDSRSITQVLDEAKRLV
CCCEECCCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
EAGVKEILVVSQDTSAYALDRSKEEQNKTVFWNGMPIKNNLISLCEQLGKLGVWVRLHYV
HHCHHHEEEEECCCCHHHHCCCHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEE
YPYPHVDQLIPLMAEGKILPYLDIPLQHASPKILKAMKRPGSIERTLERIKKWREICPDL
CCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
TLRSTFIVGFPGESEEDFQLLLDFLKEALLDRVGCFKFSPVEGAIATEMPDQVPEEVKEA
EECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHCCCHHHHHHHHHH
RFHRFMQLQQEISAARLQQKVGKVFTVLVDEVDEEGIIARSIADAPEIDGVVYIDNPNRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEECCCEE
AVKAGQFIEVKITRADAYDLYASLIN
EEECCCEEEEEEEECCHHHHHHHHCC
>Mature Secondary Structure 
TSSLPNIGFISLGCPKNLVDSERILTELRSDGYNIIPSYENADLVIVNTCGFIDSAVQE
CCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEEEECCCHHHHHHHH
SLEAIGEALEENGKVIVTGCLGAKEDRIREVHPKVLEVTGPHSYEAVMQQVHKYVPKPAY
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCC
NPYVNLVPKQGVKLTPKHYAYLKISEGCDHRCTFCIIPSMRGDLDSRSITQVLDEAKRLV
CCCEECCCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
EAGVKEILVVSQDTSAYALDRSKEEQNKTVFWNGMPIKNNLISLCEQLGKLGVWVRLHYV
HHCHHHEEEEECCCCHHHHCCCHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEE
YPYPHVDQLIPLMAEGKILPYLDIPLQHASPKILKAMKRPGSIERTLERIKKWREICPDL
CCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
TLRSTFIVGFPGESEEDFQLLLDFLKEALLDRVGCFKFSPVEGAIATEMPDQVPEEVKEA
EECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHCCCHHHHHHHHHH
RFHRFMQLQQEISAARLQQKVGKVFTVLVDEVDEEGIIARSIADAPEIDGVVYIDNPNRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEECCCEE
AVKAGQFIEVKITRADAYDLYASLIN
EEECCCEEEEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100