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The map label for this gene is pckA [H]

Identifier: 15603407

GI number: 15603407

Start: 1745289

End: 1746905

Strand: Reverse

Name: pckA [H]

Synonym: PM1542

Alternate gene names: 15603407

Gene position: 1746905-1745289 (Counterclockwise)

Preceding gene: 15603408

Following gene: 15603388

Centisome position: 77.38

GC content: 42.3

Gene sequence:

>1617_bases
ATGACTGACTTAAATAAAGTAATCAATGAACTTGGTGCACTTGGTATTCATGATGTAAAAGAAATCGTTTATAACCCAAG
TTATGAGCAATTGTTCGAAGAGGAAACCAAACCAGGTTTAGAAGGTTACGAAAAAGGTATCGTGACACAATCAGGCGCGG
TGGCGGTTGATACCGGTATCTTTACAGGTCGTTCGCCAAAAGATAAATACATTGTGCTTGATGACAAAACCAAAGACACT
GTTTGGTGGACAAGCGATGCGGCGAAAAACGATAACAAACCAATGACACAAGATACGTGGAAAAGCTTAAAAGGCTTAGT
GACAGAACAACTTTCTGGCAAACGTTTATTTGTGATCGACGCATTCTGTGGTGCAAACGCTGACACCCGTTTATCAGTGC
GTATCGTGACAGAAGTAGCATGGCAAGCACACTTTGTGAAAAACATGTTCATTCGTCCAACAGAAGCAGAATTAGTTGGC
TTCAAACCAGATTTCGTAGTAATGAACGGTTCTAAAGTAACCAACCCGAACTGGAAAGAACAAGGTCTAAATTCTGAAAA
CTTTGTGGCATTTAACTTAACTGAAGGCGTGCAATTAATCGGTGGTACTTGGTACGGCGGTGAAATGAAAAAAGGTATGT
TCTCAATGATGAACTACTTCTTACCATTAAAAGGCATCGCATCTATGCACTGTTCAGCCAACGTGGGTGAAAAAGGCGAC
GTTGCTGTGTTCTTCGGTTTATCAGGTACAGGTAAAACCACCCTTTCAACAGATCCAAAACGTCAATTAATCGGTGACGA
TGAGCACGGTTGGGATGATGATGGCGTATTCAACTACGAAGGTGGTTGCTATGCGAAAACGATCAAACTGTCTCCAGAAA
ACGAACCAGATATCTATAAAGCCATCAAACGTGATGCCTTATTAGAAAACGTTGTAGTACGTGCAGATGGTTCAGTGGAT
TACGATGATGGTTCAAAAACAGAAAACACCCGTGTTTCTTACCCAATTTACCACATCGACAACATCGTAACACCGGTATC
AAAAGCAGGTCATGCGAAAAAAGTGATCTTCTTAACTGCGGACGCATTCGGTGTGTTACCACCAGTATCTAAATTAACGC
CAGAACAAACTAAATACTACTTCTTATCTGGTTTCACCGCGAAATTAGCCGGTACTGAGCGTGGTATCACAGAACCAACA
CCAACGTTCTCTGCATGTTTCGGTGCAGCGTTCTTATCACTTCACCCAACACAATATGCGGAAGTGTTAGTGAAACGTAT
GGAAGCAGCGGGTGCGGAAGCTTACTTAGTGAACACAGGTTGGAACGGTACAGGTAAACGTATCTCAATCAAAGATACGC
GCGGTATCATCGATGCAATCTTAGACGGTTCAATCGAAAAAGCAGAAATGGGCAAATTACCAATCTTTGATTTAGCGATC
CCAACTGCATTACCAGGTGTTGACCCTGCAATCTTAGATCCACGTGATACTTATGCAGACAAAGCACAATGGCAAGCGAA
AGCAGAAGACTTAGCTGGTCGTTTCGTGAAAAACTTCGAAAAATACACCACTAACGATGAAGGTAAAGCATTAGTGGCAG
CAGGTCCAAAAGCGTAA

Upstream 100 bases:

>100_bases
TTTCAAAATTTTGATCAAGCTAACAGTTTTTAGGTAGAAATTTGATTAGAATGCTCGCATTATTTTTCAACATTTAAAAC
TTAACTCTATGAGGTAACGT

Downstream 100 bases:

>100_bases
TTAAACGCTTAACGTGATAAATAACCAAACAGCTCGATGAAAATCGGGCTGTTTTTTTATGCCACGCTTTCCCCAACCTT
AAAACTTTTCCAAAAACCAC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTDLNKVINELGALGIHDVKEIVYNPSYEQLFEEETKPGLEGYEKGIVTQSGAVAVDTGIFTGRSPKDKYIVLDDKTKDT
VWWTSDAAKNDNKPMTQDTWKSLKGLVTEQLSGKRLFVIDAFCGANADTRLSVRIVTEVAWQAHFVKNMFIRPTEAELVG
FKPDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTEGVQLIGGTWYGGEMKKGMFSMMNYFLPLKGIASMHCSANVGEKGD
VAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGVFNYEGGCYAKTIKLSPENEPDIYKAIKRDALLENVVVRADGSVD
YDDGSKTENTRVSYPIYHIDNIVTPVSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPT
PTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGKLPIFDLAI
PTALPGVDPAILDPRDTYADKAQWQAKAEDLAGRFVKNFEKYTTNDEGKALVAAGPKA

Sequences:

>Translated_538_residues
MTDLNKVINELGALGIHDVKEIVYNPSYEQLFEEETKPGLEGYEKGIVTQSGAVAVDTGIFTGRSPKDKYIVLDDKTKDT
VWWTSDAAKNDNKPMTQDTWKSLKGLVTEQLSGKRLFVIDAFCGANADTRLSVRIVTEVAWQAHFVKNMFIRPTEAELVG
FKPDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTEGVQLIGGTWYGGEMKKGMFSMMNYFLPLKGIASMHCSANVGEKGD
VAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGVFNYEGGCYAKTIKLSPENEPDIYKAIKRDALLENVVVRADGSVD
YDDGSKTENTRVSYPIYHIDNIVTPVSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPT
PTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGKLPIFDLAI
PTALPGVDPAILDPRDTYADKAQWQAKAEDLAGRFVKNFEKYTTNDEGKALVAAGPKA
>Mature_537_residues
TDLNKVINELGALGIHDVKEIVYNPSYEQLFEEETKPGLEGYEKGIVTQSGAVAVDTGIFTGRSPKDKYIVLDDKTKDTV
WWTSDAAKNDNKPMTQDTWKSLKGLVTEQLSGKRLFVIDAFCGANADTRLSVRIVTEVAWQAHFVKNMFIRPTEAELVGF
KPDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTEGVQLIGGTWYGGEMKKGMFSMMNYFLPLKGIASMHCSANVGEKGDV
AVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGVFNYEGGCYAKTIKLSPENEPDIYKAIKRDALLENVVVRADGSVDY
DDGSKTENTRVSYPIYHIDNIVTPVSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTP
TFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGKLPIFDLAIP
TALPGVDPAILDPRDTYADKAQWQAKAEDLAGRFVKNFEKYTTNDEGKALVAAGPKA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=530, Percent_Identity=80.9433962264151, Blast_Score=901, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=504, Percent_Identity=47.0238095238095, Blast_Score=453, Evalue=1e-128,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 58880; Mature: 58749

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLNKVINELGALGIHDVKEIVYNPSYEQLFEEETKPGLEGYEKGIVTQSGAVAVDTGI
CCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCEECCCCEEEECCE
FTGRSPKDKYIVLDDKTKDTVWWTSDAAKNDNKPMTQDTWKSLKGLVTEQLSGKRLFVID
ECCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
AFCGANADTRLSVRIVTEVAWQAHFVKNMFIRPTEAELVGFKPDFVVMNGSKVTNPNWKE
ECCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEECCCEECCCCHHH
QGLNSENFVAFNLTEGVQLIGGTWYGGEMKKGMFSMMNYFLPLKGIASMHCSANVGEKGD
HCCCCCCEEEEEECCCCEEECCEEECCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCC
VAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGVFNYEGGCYAKTIKLSPENEPDIYK
EEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCEEEEEEEECCCCCCHHHH
AIKRDALLENVVVRADGSVDYDDGSKTENTRVSYPIYHIDNIVTPVSKAGHAKKVIFLTA
HHHHHHHHHHEEEEECCCEECCCCCCCCCCEEEEEEHHHHHHHCCHHCCCCCEEEEEEEE
DAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLSLHPTQYA
CCCCCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
EVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGKLPIFDLAI
HHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHCCCCCEEEEEC
PTALPGVDPAILDPRDTYADKAQWQAKAEDLAGRFVKNFEKYTTNDEGKALVAAGPKA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
>Mature Secondary Structure 
TDLNKVINELGALGIHDVKEIVYNPSYEQLFEEETKPGLEGYEKGIVTQSGAVAVDTGI
CHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCEECCCCEEEECCE
FTGRSPKDKYIVLDDKTKDTVWWTSDAAKNDNKPMTQDTWKSLKGLVTEQLSGKRLFVID
ECCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
AFCGANADTRLSVRIVTEVAWQAHFVKNMFIRPTEAELVGFKPDFVVMNGSKVTNPNWKE
ECCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEECCCEECCCCHHH
QGLNSENFVAFNLTEGVQLIGGTWYGGEMKKGMFSMMNYFLPLKGIASMHCSANVGEKGD
HCCCCCCEEEEEECCCCEEECCEEECCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCC
VAVFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDDGVFNYEGGCYAKTIKLSPENEPDIYK
EEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCEEEEEEEECCCCCCHHHH
AIKRDALLENVVVRADGSVDYDDGSKTENTRVSYPIYHIDNIVTPVSKAGHAKKVIFLTA
HHHHHHHHHHEEEEECCCEECCCCCCCCCCEEEEEEHHHHHHHCCHHCCCCCEEEEEEEE
DAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLSLHPTQYA
CCCCCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
EVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGKLPIFDLAI
HHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHCCCCCEEEEEC
PTALPGVDPAILDPRDTYADKAQWQAKAEDLAGRFVKNFEKYTTNDEGKALVAAGPKA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA