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The map label for this gene is hpaB [H]

Identifier: 15603388

GI number: 15603388

Start: 1721515

End: 1723113

Strand: Reverse

Name: hpaB [H]

Synonym: PM1523

Alternate gene names: 15603388

Gene position: 1723113-1721515 (Counterclockwise)

Preceding gene: 15603407

Following gene: 15603387

Centisome position: 76.33

GC content: 43.4

Gene sequence:

>1599_bases
ATGACGCAAGCAGAAAAAAAACAGCACAAATTACCGGATGATTACCGTAAAGAAAAAAATCGTCCATTCACCGGTGAAGA
ATATTTAGCAAGTTTAAGAGATGGGCGCAAAGTCTATATTTATGGTGAGTTAGTCGATGACGTGACAACCCACCCTGCAT
TTCGTAATGCCGCCGCCTCTGTGGCAAAACTCTACGATGCGTTACATGATCCAGCAACCAAAGACGAATTGTGTTGGGAA
ACAGATACTGGCAATGGCGGTTATACCCATAAATTCTTCCGTTATGCCCGTACCGCAGAAGAACTCAAAGCACAACGCGA
TGCTATCGCAGCTTGGTCACGTTTTAGCTATGGCTGGATGGGGCGTACTCCAGACTATAAAGCGGCATTTGGTAGCGCGT
TAGGCGCCAATCCAGAATTTTACGGTAAGTTCGCCGATAATGCGCGCAATTGGTATAAACGTATCCAAGAATCTTGCCTC
TATTTCAACCACGCTATCGTTAATCCACCGATTGACCGTAACAAACCGATTGATCAAGTTAAAGATATCTATGTGAAAAT
CGAAGAAGAACGTGCCGATGGGATTGTGGTCAGTGGGGCGAAAGTGGTGGCGACTAACTCAGCACTGACTCACTATAACT
TTATTGGATTTGGTTCCGCACAAATTATTGGTGATAACGACGACTTTGCCTTGATGTTTATTGCACCCATGGATGCAGAA
GGTGTCAAACTCATTTGCCGTCACTCTTATGAACTTGCCGCAGGCGCGACCGGTTCACCGTTTGACTTCCCACTTTCTAC
TCGTTTCGATGAAAACGATGCCATTTTGGTGTTAGACAGCGTCTTTATTCCTTGGGAAAACATTTTAATCTATCGTGATC
TAGAGCGTTGTCGTACTTGGGCACAATATGCCGGCTTTGCAACGCTTTTCCCAATGCAAGCTTGTACCCGTTTGGCGGTG
AAATTAGATTTCATTACAGGTTTATTACATAAAGCATTACAATGTACTGGCGTGATTGAATTCCGTGGCGTCGCGGCACA
AATGGGCGAAGTGATGATGTATCGCAACCTCTTTTGGACACTTACTGATGCCATGGCGGGATCTTCTTATCAGTGGAAAA
ATGGTTGCCAAATGCCAAGTGCACAAGCAATCCAAACCTATCGCGTGATGGCACCAATGGCCTACCATACGGTGAAAAAA
ATCATTGAACAAACGGTAACAAGTGGGTTAATTTATTTGCCTTCACATATTCGCGATTTCAATAACCCTGAAATCGACCA
ATATTTAGAAAAATACGTGCGTGGTTCAAACAATATTGAATACCGTGATCGTATCAAAATTTTAAAACTTCTGTGGGATG
CCATTGGCACGGAGTTCGGTGGTCGTCACGAACTTTACGAAATCAACTACGCCGGTAGCCAAGAAGAAGTGCGTTTACAA
GTACTCGGGCAATCCACCGGTAACGGTACATTACAGCGTTGTTTCGATTTAGTGGATAAATGCTTGGCTGACTATGATGA
AAAAGGTTGGAAAGTGCCACATCTGAATAACTCAGAAGATATTAATATTTTGCATAACCTCAACCGTTTTTCAAAATAA

Upstream 100 bases:

>100_bases
TCTTGCGCAGAGATCCACTATAAATTTAACAAATTCGCAACATGCCAGCGATTTTGCCCGAAATGCTATTCAACCCTTAT
TTATTCAAAAGGAGTAAATC

Downstream 100 bases:

>100_bases
CGTACGTTGTAAAACATAAGGAAAATCAATATGCTTACGCCAATTCAACAACACTTTCGTAATTCCATGGCAAAATTGGC
CGCTGGCGTGAATATTATTA

Product: hypothetical protein

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTQAEKKQHKLPDDYRKEKNRPFTGEEYLASLRDGRKVYIYGELVDDVTTHPAFRNAAASVAKLYDALHDPATKDELCWE
TDTGNGGYTHKFFRYARTAEELKAQRDAIAAWSRFSYGWMGRTPDYKAAFGSALGANPEFYGKFADNARNWYKRIQESCL
YFNHAIVNPPIDRNKPIDQVKDIYVKIEEERADGIVVSGAKVVATNSALTHYNFIGFGSAQIIGDNDDFALMFIAPMDAE
GVKLICRHSYELAAGATGSPFDFPLSTRFDENDAILVLDSVFIPWENILIYRDLERCRTWAQYAGFATLFPMQACTRLAV
KLDFITGLLHKALQCTGVIEFRGVAAQMGEVMMYRNLFWTLTDAMAGSSYQWKNGCQMPSAQAIQTYRVMAPMAYHTVKK
IIEQTVTSGLIYLPSHIRDFNNPEIDQYLEKYVRGSNNIEYRDRIKILKLLWDAIGTEFGGRHELYEINYAGSQEEVRLQ
VLGQSTGNGTLQRCFDLVDKCLADYDEKGWKVPHLNNSEDINILHNLNRFSK

Sequences:

>Translated_532_residues
MTQAEKKQHKLPDDYRKEKNRPFTGEEYLASLRDGRKVYIYGELVDDVTTHPAFRNAAASVAKLYDALHDPATKDELCWE
TDTGNGGYTHKFFRYARTAEELKAQRDAIAAWSRFSYGWMGRTPDYKAAFGSALGANPEFYGKFADNARNWYKRIQESCL
YFNHAIVNPPIDRNKPIDQVKDIYVKIEEERADGIVVSGAKVVATNSALTHYNFIGFGSAQIIGDNDDFALMFIAPMDAE
GVKLICRHSYELAAGATGSPFDFPLSTRFDENDAILVLDSVFIPWENILIYRDLERCRTWAQYAGFATLFPMQACTRLAV
KLDFITGLLHKALQCTGVIEFRGVAAQMGEVMMYRNLFWTLTDAMAGSSYQWKNGCQMPSAQAIQTYRVMAPMAYHTVKK
IIEQTVTSGLIYLPSHIRDFNNPEIDQYLEKYVRGSNNIEYRDRIKILKLLWDAIGTEFGGRHELYEINYAGSQEEVRLQ
VLGQSTGNGTLQRCFDLVDKCLADYDEKGWKVPHLNNSEDINILHNLNRFSK
>Mature_531_residues
TQAEKKQHKLPDDYRKEKNRPFTGEEYLASLRDGRKVYIYGELVDDVTTHPAFRNAAASVAKLYDALHDPATKDELCWET
DTGNGGYTHKFFRYARTAEELKAQRDAIAAWSRFSYGWMGRTPDYKAAFGSALGANPEFYGKFADNARNWYKRIQESCLY
FNHAIVNPPIDRNKPIDQVKDIYVKIEEERADGIVVSGAKVVATNSALTHYNFIGFGSAQIIGDNDDFALMFIAPMDAEG
VKLICRHSYELAAGATGSPFDFPLSTRFDENDAILVLDSVFIPWENILIYRDLERCRTWAQYAGFATLFPMQACTRLAVK
LDFITGLLHKALQCTGVIEFRGVAAQMGEVMMYRNLFWTLTDAMAGSSYQWKNGCQMPSAQAIQTYRVMAPMAYHTVKKI
IEQTVTSGLIYLPSHIRDFNNPEIDQYLEKYVRGSNNIEYRDRIKILKLLWDAIGTEFGGRHELYEINYAGSQEEVRLQV
LGQSTGNGTLQRCFDLVDKCLADYDEKGWKVPHLNNSEDINILHNLNRFSK

Specific function: Utilizes FADH(2) supplied by hpaC or by another flavin reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-DHPA. Can also oxidize phenol to catechol, and hydroxylate other phenol derivatives [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012688 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 60589; Mature: 60458

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAEKKQHKLPDDYRKEKNRPFTGEEYLASLRDGRKVYIYGELVDDVTTHPAFRNAAAS
CCCCCHHHCCCCHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEEHHHHHCCCHHHHHHHH
VAKLYDALHDPATKDELCWETDTGNGGYTHKFFRYARTAEELKAQRDAIAAWSRFSYGWM
HHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GRTPDYKAAFGSALGANPEFYGKFADNARNWYKRIQESCLYFNHAIVNPPIDRNKPIDQV
CCCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
KDIYVKIEEERADGIVVSGAKVVATNSALTHYNFIGFGSAQIIGDNDDFALMFIAPMDAE
HHHHEEEEECCCCEEEEECCEEEEECCCCCEEEEEECCCEEEECCCCCEEEEEEECCCCC
GVKLICRHSYELAAGATGSPFDFPLSTRFDENDAILVLDSVFIPWENILIYRDLERCRTW
CHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEECEECCCCCEEEEECHHHHHHH
AQYAGFATLFPMQACTRLAVKLDFITGLLHKALQCTGVIEFRGVAAQMGEVMMYRNLFWT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LTDAMAGSSYQWKNGCQMPSAQAIQTYRVMAPMAYHTVKKIIEQTVTSGLIYLPSHIRDF
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHCCC
NNPEIDQYLEKYVRGSNNIEYRDRIKILKLLWDAIGTEFGGRHELYEINYAGSQEEVRLQ
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEE
VLGQSTGNGTLQRCFDLVDKCLADYDEKGWKVPHLNNSEDINILHNLNRFSK
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TQAEKKQHKLPDDYRKEKNRPFTGEEYLASLRDGRKVYIYGELVDDVTTHPAFRNAAAS
CCCCHHHCCCCHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEEHHHHHCCCHHHHHHHH
VAKLYDALHDPATKDELCWETDTGNGGYTHKFFRYARTAEELKAQRDAIAAWSRFSYGWM
HHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GRTPDYKAAFGSALGANPEFYGKFADNARNWYKRIQESCLYFNHAIVNPPIDRNKPIDQV
CCCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
KDIYVKIEEERADGIVVSGAKVVATNSALTHYNFIGFGSAQIIGDNDDFALMFIAPMDAE
HHHHEEEEECCCCEEEEECCEEEEECCCCCEEEEEECCCEEEECCCCCEEEEEEECCCCC
GVKLICRHSYELAAGATGSPFDFPLSTRFDENDAILVLDSVFIPWENILIYRDLERCRTW
CHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEECEECCCCCEEEEECHHHHHHH
AQYAGFATLFPMQACTRLAVKLDFITGLLHKALQCTGVIEFRGVAAQMGEVMMYRNLFWT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LTDAMAGSSYQWKNGCQMPSAQAIQTYRVMAPMAYHTVKKIIEQTVTSGLIYLPSHIRDF
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHCCC
NNPEIDQYLEKYVRGSNNIEYRDRIKILKLLWDAIGTEFGGRHELYEINYAGSQEEVRLQ
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEE
VLGQSTGNGTLQRCFDLVDKCLADYDEKGWKVPHLNNSEDINILHNLNRFSK
EEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10653707 [H]