Click here to switch to the map view.

The map label for this gene is gidA

Identifier: 15603350

GI number: 15603350

Start: 1677605

End: 1679494

Strand: Direct

Name: gidA

Synonym: PM1485

Alternate gene names: 15603350

Gene position: 1677605-1679494 (Clockwise)

Preceding gene: 15603349

Following gene: 15603351

Centisome position: 74.31

GC content: 40.26

Gene sequence:

>1890_bases
ATGTTTTATACTGAAAATTATGATGTTATTGTGATCGGTGGTGGACACGCAGGTACTGAAGCTGCACTTGCACCGGCACG
CATGGGACTCAAGACCCTATTATTGACCCATAACGTTGATACACTAGGGCAAATGTCTTGTAATCCTGCGATTGGTGGGA
TTGGTAAAGGTCATTTAGTCCGAGAAATTGATGCGATGGGCGGTTTAATGGCAACTGCTGCGGACCAAGCAGGAATCCAA
TTTCGTACCTTAAACAGTAGCAAAGGACCGGCGGTACGTGCTACACGTGCGCAAGCTGACCGCGTTTTATATCGCCAAGC
AGTACGTATTGCATTAGAAAATCAAGAAAATTTAGATATTTTTCAACAAGAAGTGACCGATATTATTTTAGATCAGGATC
GTGTCTGCGGTGTTGTTACTAAAATGGGTTTAAAATTTCATGCAAAAGCAGTGATTTTAACAGCCGGTACTTTCCTTTCT
GGTAAGATCCACATTGGTTTAGAAAATTATACAGGTGGACGCGCGGGTGATCCTGCTTCAGTGATGTTAGCCGATCGTTT
AAGAGAACTGAATTTACGTGTAGATCGTTTAAAAACGGGTACACCGCCCCGTATTGATGCACGTACTATTGATTTCTCAG
TACTGGCTAAACAACATGGCGATGAAAAATTACCTGTCTTTTCCTTCATGGGATCTGTTGATCAACACCCACGTCAAATT
CCATGTTTTATTACCCATACAAATGAACAAACGCATGAAGTGATCCGTAATAACTTAGATCGCAGCCCAATGTATGCTGG
GATCATTGAAGGGATCGGTCCACGTTATTGCCCTTCTATTGAAGATAAAGTAATGCGTTTTTCTGAGCGTAATTCTCATC
AAATCTACCTTGAACCTGAAGGGTTAACCAGTAACGAGATCTATCCAAATGGGATCTCCACCAGTTTACCTTTTGATGTT
CAAATGAAGATCGTTAATTCAATGAAAGGGATGGAAAAAGCACGCATTATCAAGCCAGGTTATGCAATTGAATACGACTA
TTTTGATCCAAGAGATCTCAAACCCACTTTAGAAACAAAATCGATCCGTGGTTTATTTTTTGCTGGTCAAATTAACGGAA
CCACAGGTTATGAAGAAGCCGCAGGGCAAGGTTTACTGGCAGGAATTAATGCTGGGTTATTTGTACAAGAAAAAGAAGCT
TGGTTCCCACGTCGTGATCAAGCATATATCGGTGTTCTTGTTGATGATCTTTGTACTTTAGGGACGAAAGAACCTTATCG
TGTATTTACGTCTCGTGCAGAATATCGCTTATTGTTGCGTGAAGATAATGCTGATAGTCGTTTAACACCAATTGCTCATC
AATTAGGTTTAATTGATGAAAAACGTTGGGCAAGATTCAATCAAAAAATGGAAAATATTGAATTAGAAAGACAACGTTTA
CGCCAAATTTGGCTCCACCCACGTTCTGAATATTTAGACGAAGCAAATAAGGTCTTAGGTAGTCCATTAGTGCGTGAAGC
CAGTGGGGAAGATTTATTACGTCGCCCAGAAATGAATTATCAGATTTTAACTTCATTAACGCCATTCCAACCAGCAATGG
ATGATCAAGAGGCGGTAGAACAAGTAGAAATTGCGATTAAATATCAAGGTTATATTGAACATCAACAAGAAGAAATTGCA
CGTCAAAAACGGCATGAAAGTACCGCGATTCCTGCTCATTTTGATTATACTGTCGTATCTGGTTTATCCAATGAAGTACG
TGCTAAGTTAGAGCAACATCGCCCTGTTTCGATTGGACAAGCTTCGCGTATTTCTGGTGTGACTCCAGCGGCGATTTCAA
TTTTATTAGTAAGCTTGAAAAAACAAGGCATGTTAAAGCGTGGTGAATAA

Upstream 100 bases:

>100_bases
AAAATGTTTCTTTCATGGATCCATAAATTTCAGTAGAATAGCCAACCAGCAAAAAGGATCAAAAGATCCATAAAATCCGA
GATAAATTAACAAGGTTACT

Downstream 100 bases:

>100_bases
TGACTAACCTTGAGCAACAACTTAGCCAAAAATTAGAAATTTTATTAAAACAGACCGCACTTTCGATAACCGATCAACAG
AAAGAGCAGTTAATAAAATT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MFYTENYDVIVIGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVREIDAMGGLMATAADQAGIQ
FRTLNSSKGPAVRATRAQADRVLYRQAVRIALENQENLDIFQQEVTDIILDQDRVCGVVTKMGLKFHAKAVILTAGTFLS
GKIHIGLENYTGGRAGDPASVMLADRLRELNLRVDRLKTGTPPRIDARTIDFSVLAKQHGDEKLPVFSFMGSVDQHPRQI
PCFITHTNEQTHEVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFSERNSHQIYLEPEGLTSNEIYPNGISTSLPFDV
QMKIVNSMKGMEKARIIKPGYAIEYDYFDPRDLKPTLETKSIRGLFFAGQINGTTGYEEAAGQGLLAGINAGLFVQEKEA
WFPRRDQAYIGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADSRLTPIAHQLGLIDEKRWARFNQKMENIELERQRL
RQIWLHPRSEYLDEANKVLGSPLVREASGEDLLRRPEMNYQILTSLTPFQPAMDDQEAVEQVEIAIKYQGYIEHQQEEIA
RQKRHESTAIPAHFDYTVVSGLSNEVRAKLEQHRPVSIGQASRISGVTPAAISILLVSLKKQGMLKRGE

Sequences:

>Translated_629_residues
MFYTENYDVIVIGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVREIDAMGGLMATAADQAGIQ
FRTLNSSKGPAVRATRAQADRVLYRQAVRIALENQENLDIFQQEVTDIILDQDRVCGVVTKMGLKFHAKAVILTAGTFLS
GKIHIGLENYTGGRAGDPASVMLADRLRELNLRVDRLKTGTPPRIDARTIDFSVLAKQHGDEKLPVFSFMGSVDQHPRQI
PCFITHTNEQTHEVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFSERNSHQIYLEPEGLTSNEIYPNGISTSLPFDV
QMKIVNSMKGMEKARIIKPGYAIEYDYFDPRDLKPTLETKSIRGLFFAGQINGTTGYEEAAGQGLLAGINAGLFVQEKEA
WFPRRDQAYIGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADSRLTPIAHQLGLIDEKRWARFNQKMENIELERQRL
RQIWLHPRSEYLDEANKVLGSPLVREASGEDLLRRPEMNYQILTSLTPFQPAMDDQEAVEQVEIAIKYQGYIEHQQEEIA
RQKRHESTAIPAHFDYTVVSGLSNEVRAKLEQHRPVSIGQASRISGVTPAAISILLVSLKKQGMLKRGE
>Mature_629_residues
MFYTENYDVIVIGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVREIDAMGGLMATAADQAGIQ
FRTLNSSKGPAVRATRAQADRVLYRQAVRIALENQENLDIFQQEVTDIILDQDRVCGVVTKMGLKFHAKAVILTAGTFLS
GKIHIGLENYTGGRAGDPASVMLADRLRELNLRVDRLKTGTPPRIDARTIDFSVLAKQHGDEKLPVFSFMGSVDQHPRQI
PCFITHTNEQTHEVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFSERNSHQIYLEPEGLTSNEIYPNGISTSLPFDV
QMKIVNSMKGMEKARIIKPGYAIEYDYFDPRDLKPTLETKSIRGLFFAGQINGTTGYEEAAGQGLLAGINAGLFVQEKEA
WFPRRDQAYIGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADSRLTPIAHQLGLIDEKRWARFNQKMENIELERQRL
RQIWLHPRSEYLDEANKVLGSPLVREASGEDLLRRPEMNYQILTSLTPFQPAMDDQEAVEQVEIAIKYQGYIEHQQEEIA
RQKRHESTAIPAHFDYTVVSGLSNEVRAKLEQHRPVSIGQASRISGVTPAAISILLVSLKKQGMLKRGE

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=643, Percent_Identity=46.9673405909798, Blast_Score=516, Evalue=1e-146,
Organism=Homo sapiens, GI19882217, Length=668, Percent_Identity=45.2095808383234, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI183227703, Length=683, Percent_Identity=44.2166910688141, Blast_Score=498, Evalue=1e-141,
Organism=Escherichia coli, GI2367273, Length=627, Percent_Identity=76.3955342902711, Blast_Score=1010, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=632, Percent_Identity=38.7658227848101, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6321202, Length=636, Percent_Identity=44.811320754717, Blast_Score=501, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24658174, Length=636, Percent_Identity=44.6540880503145, Blast_Score=501, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_PASMU (P57945)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246424.1
- ProteinModelPortal:   P57945
- SMR:   P57945
- PRIDE:   P57945
- GeneID:   1244832
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1485
- NMPDR:   fig|272843.1.peg.1486
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   PMUL272843:PM1485-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70325; Mature: 70325

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 125-125 BINDING 180-180 BINDING 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYTENYDVIVIGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQMSCNPAIGGIGKGHLV
CCEECCCCEEEEECCCCCCHHHHHHHHHCHHHEEEECCCCHHHCCCCCCCCCCCCCCHHH
REIDAMGGLMATAADQAGIQFRTLNSSKGPAVRATRAQADRVLYRQAVRIALENQENLDI
HHHHHHCCHHHHHHHHCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHH
FQQEVTDIILDQDRVCGVVTKMGLKFHAKAVILTAGTFLSGKIHIGLENYTGGRAGDPAS
HHHHHHHHHHCCCHHHHHHHHHCCCEECEEEEEEECCEEECEEEEEEECCCCCCCCCCHH
VMLADRLRELNLRVDRLKTGTPPRIDARTIDFSVLAKQHGDEKLPVFSFMGSVDQHPRQI
HHHHHHHHHHCCEEEHHCCCCCCCCCEEEEHHHHHHHHCCCCCCCHHHHHCCCCCCCCCC
PCFITHTNEQTHEVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFSERNSHQIYLEPE
CEEEECCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMKIVNSMKGMEKARIIKPGYAIEYDYFDPRDLKPTLETK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHEEECCCCEEEECCCCCCCCCCCCCCC
SIRGLFFAGQINGTTGYEEAAGQGLLAGINAGLFVQEKEAWFPRRDQAYIGVLVDDLCTL
CCCEEEEEEEECCCCCCHHHCCCCEEEECCCCEEEEEHHCCCCCCCCCEEHHHHHHHHHC
GTKEPYRVFTSRAEYRLLLREDNADSRLTPIAHQLGLIDEKRWARFNQKMENIELERQRL
CCCCCHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RQIWLHPRSEYLDEANKVLGSPLVREASGEDLLRRPEMNYQILTSLTPFQPAMDDQEAVE
HHHHCCCCHHHHHHHHHHHCCCHHHCCCCCHHHHCCCCCEEEEECCCCCCCCCCHHHHHH
QVEIAIKYQGYIEHQQEEIARQKRHESTAIPAHFDYTVVSGLSNEVRAKLEQHRPVSIGQ
HEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCC
ASRISGVTPAAISILLVSLKKQGMLKRGE
CCCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFYTENYDVIVIGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQMSCNPAIGGIGKGHLV
CCEECCCCEEEEECCCCCCHHHHHHHHHCHHHEEEECCCCHHHCCCCCCCCCCCCCCHHH
REIDAMGGLMATAADQAGIQFRTLNSSKGPAVRATRAQADRVLYRQAVRIALENQENLDI
HHHHHHCCHHHHHHHHCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHH
FQQEVTDIILDQDRVCGVVTKMGLKFHAKAVILTAGTFLSGKIHIGLENYTGGRAGDPAS
HHHHHHHHHHCCCHHHHHHHHHCCCEECEEEEEEECCEEECEEEEEEECCCCCCCCCCHH
VMLADRLRELNLRVDRLKTGTPPRIDARTIDFSVLAKQHGDEKLPVFSFMGSVDQHPRQI
HHHHHHHHHHCCEEEHHCCCCCCCCCEEEEHHHHHHHHCCCCCCCHHHHHCCCCCCCCCC
PCFITHTNEQTHEVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFSERNSHQIYLEPE
CEEEECCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMKIVNSMKGMEKARIIKPGYAIEYDYFDPRDLKPTLETK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHEEECCCCEEEECCCCCCCCCCCCCCC
SIRGLFFAGQINGTTGYEEAAGQGLLAGINAGLFVQEKEAWFPRRDQAYIGVLVDDLCTL
CCCEEEEEEEECCCCCCHHHCCCCEEEECCCCEEEEEHHCCCCCCCCCEEHHHHHHHHHC
GTKEPYRVFTSRAEYRLLLREDNADSRLTPIAHQLGLIDEKRWARFNQKMENIELERQRL
CCCCCHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RQIWLHPRSEYLDEANKVLGSPLVREASGEDLLRRPEMNYQILTSLTPFQPAMDDQEAVE
HHHHCCCCHHHHHHHHHHHCCCHHHCCCCCHHHHCCCCCEEEEECCCCCCCCCCHHHHHH
QVEIAIKYQGYIEHQQEEIARQKRHESTAIPAHFDYTVVSGLSNEVRAKLEQHRPVSIGQ
HEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCC
ASRISGVTPAAISILLVSLKKQGMLKRGE
CCCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100