Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 15603351

GI number: 15603351

Start: 1679494

End: 1680126

Strand: Direct

Name: gidB

Synonym: PM1486

Alternate gene names: 15603351

Gene position: 1679494-1680126 (Clockwise)

Preceding gene: 15603350

Following gene: 15603352

Centisome position: 74.4

GC content: 34.91

Gene sequence:

>633_bases
ATGACTAACCTTGAGCAACAACTTAGCCAAAAATTAGAAATTTTATTAAAACAGACCGCACTTTCGATAACCGATCAACA
GAAAGAGCAGTTAATAAAATTGGTTTTATTATTAAATAAGTGGAATAAAGCCTATAATCTCACTTCTGTACGTGATCCGA
TGGAAATGTTGGTAAAGCATATCTTAGACAGTGTTGTGGTGAGCCCTCATTTACAAGGTAAACATTTTATTGATGTTGGT
ACAGGTCCTGGCTTACCGGGATTACCGCTTGCTATTGTTAATCCTAATAAACACTTTGTACTTTTAGACAGTTTGGGTAA
ACGAATTAGTTTTATTCGTAATGCGATTCGTGAGTTAGGCTTAGATAATGTTGAAGCTGTACTAAGCCGAGTTGAAGAAT
ATCATCCAGAACAACCATTTGATGGTGTATTAAGTCGTGCTTTTGCTTCTTTAAAAGATATGACGGATTGGTGTCAACAT
TTACCAAAACAAGATGGTTATTTTTATGCTTTAAAAGGGCTTTACCATCAAGAAGAAGTGGAAGAATTAAGTGAAAAGTT
TACTATTCAACAGGTGATTCGACTACAAGTGCCAGAGCTGATTGGAGAAAGACATTTAGTGATTGTTAAGTAA

Upstream 100 bases:

>100_bases
CGATTGGACAAGCTTCGCGTATTTCTGGTGTGACTCCAGCGGCGATTTCAATTTTATTAGTAAGCTTGAAAAAACAAGGC
ATGTTAAAGCGTGGTGAATA

Downstream 100 bases:

>100_bases
ACTTTAAAGGAATGTTTAAAAAATATAATTTTGTGATCTTGTTCACATATTTTAATTTCTAATAGTATTAAAACTAGCGT
ATAATCCACAGCAATTTTGT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTNLEQQLSQKLEILLKQTALSITDQQKEQLIKLVLLLNKWNKAYNLTSVRDPMEMLVKHILDSVVVSPHLQGKHFIDVG
TGPGLPGLPLAIVNPNKHFVLLDSLGKRISFIRNAIRELGLDNVEAVLSRVEEYHPEQPFDGVLSRAFASLKDMTDWCQH
LPKQDGYFYALKGLYHQEEVEELSEKFTIQQVIRLQVPELIGERHLVIVK

Sequences:

>Translated_210_residues
MTNLEQQLSQKLEILLKQTALSITDQQKEQLIKLVLLLNKWNKAYNLTSVRDPMEMLVKHILDSVVVSPHLQGKHFIDVG
TGPGLPGLPLAIVNPNKHFVLLDSLGKRISFIRNAIRELGLDNVEAVLSRVEEYHPEQPFDGVLSRAFASLKDMTDWCQH
LPKQDGYFYALKGLYHQEEVEELSEKFTIQQVIRLQVPELIGERHLVIVK
>Mature_209_residues
TNLEQQLSQKLEILLKQTALSITDQQKEQLIKLVLLLNKWNKAYNLTSVRDPMEMLVKHILDSVVVSPHLQGKHFIDVGT
GPGLPGLPLAIVNPNKHFVLLDSLGKRISFIRNAIRELGLDNVEAVLSRVEEYHPEQPFDGVLSRAFASLKDMTDWCQHL
PKQDGYFYALKGLYHQEEVEELSEKFTIQQVIRLQVPELIGERHLVIVK

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=201, Percent_Identity=63.18407960199, Blast_Score=271, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_PASMU (P57946)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246425.1
- ProteinModelPortal:   P57946
- SMR:   P57946
- GeneID:   1244833
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1486
- NMPDR:   fig|272843.1.peg.1487
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- BioCyc:   PMUL272843:PM1486-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 24008; Mature: 23877

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLEQQLSQKLEILLKQTALSITDQQKEQLIKLVLLLNKWNKAYNLTSVRDPMEMLVKH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHH
ILDSVVVSPHLQGKHFIDVGTGPGLPGLPLAIVNPNKHFVLLDSLGKRISFIRNAIRELG
HHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHCC
LDNVEAVLSRVEEYHPEQPFDGVLSRAFASLKDMTDWCQHLPKQDGYFYALKGLYHQEEV
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
EELSEKFTIQQVIRLQVPELIGERHLVIVK
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
TNLEQQLSQKLEILLKQTALSITDQQKEQLIKLVLLLNKWNKAYNLTSVRDPMEMLVKH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHH
ILDSVVVSPHLQGKHFIDVGTGPGLPGLPLAIVNPNKHFVLLDSLGKRISFIRNAIRELG
HHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHCC
LDNVEAVLSRVEEYHPEQPFDGVLSRAFASLKDMTDWCQHLPKQDGYFYALKGLYHQEEV
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
EELSEKFTIQQVIRLQVPELIGERHLVIVK
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100