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The map label for this gene is ygbJ [C]

Identifier: 15603231

GI number: 15603231

Start: 1557064

End: 1557969

Strand: Reverse

Name: ygbJ [C]

Synonym: PM1366

Alternate gene names: 15603231

Gene position: 1557969-1557064 (Counterclockwise)

Preceding gene: 15603233

Following gene: 15603230

Centisome position: 69.01

GC content: 46.47

Gene sequence:

>906_bases
ATGAATCAAAATATTTCAGTCGCCGTGATTGGCTTAGGTGCAATGGGAATGGGCGCCGCGCAATCTTGTATTCGAGCAGG
ATTAACAACTTACGGTGCAGATTTGAACCCGCTTGCCTTGGAGAAACTTAAAGCCGAGGGAGCAAAGGGCGTATCACAAA
GTGCGGTGGATTTTGCACAAGAATTAGATGCGGTGTTGTTGTTAGTTGTCAATGCTAGCCAAGTGAATACAGTGCTATTT
GGCGAGAAAGGCTTGGCAAGTCATTTAAAACCAGGTACTGCGGTGATGGTCTCTTCTACCATTTCTGCACAAGATGCGCA
GCAGATTTCACAAAAATTAACAGGGCTAGGGTTGGTGATGTTAGATGCGCCTGTTTCTGGGGGGGCGGCAAAAGCGGCGG
CAGGCGAGATGACCGTGATGGCATCTGGTTCTCAACAAGCTTTTACGAAATTGCAACCAGTCTTAGATGCGGTGGCGGGT
AAAGTGTATAACATCGGTGAAGAAATTGGCTTAGGGGCAACCGTGAAAATTATTCACCAATTACTCGCAGGGGTACATAT
TGCCGCGGGTGCAGAAGCCATGGCATTAGCGGCTAGAGCTGGTATTCCACTCGATTTAATGTATGACGTGGTGACTCATG
CTGCAGGGAATTCTTGGATGTTTGAAAATCGTATGAAGCACGTTGTAGAGGGCGACTATACCCCACTGTCTATGGTAGAT
ATTTTTGTGAAAGATTTAGGTCTTGTCACGGATACTGCAAAAGCCTTGAAGTTCCCGTTACCGCTAGCAAGCACGGCGTT
TAATATGTTTACGTCAGCCAGCAATGCGGGGTATGGCAAAGAAGATGATAGTGCGGTCATTAAGATTTTTAGTGGCATTG
AATTACCACAGAAAAAAGGAGCGTGA

Upstream 100 bases:

>100_bases
TGATTTGTTAAATTTTGAGATCTCGATCGCAGATTTATTTTATAAAAGGAGGCAATATAGTGTTAGTCGAACACCCGTTA
AAATCTGAGTGGAGGAACTT

Downstream 100 bases:

>100_bases
TTATGTTAGGTGTCATTGCAGATGATTTTACTGGTGCGAGTGATATTGCCAGTTTTCTAGTGGAAAATGGGCTTGCTACC
GTACAGATGAATGGGGTGCC

Product: hypothetical protein

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MNQNISVAVIGLGAMGMGAAQSCIRAGLTTYGADLNPLALEKLKAEGAKGVSQSAVDFAQELDAVLLLVVNASQVNTVLF
GEKGLASHLKPGTAVMVSSTISAQDAQQISQKLTGLGLVMLDAPVSGGAAKAAAGEMTVMASGSQQAFTKLQPVLDAVAG
KVYNIGEEIGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTHAAGNSWMFENRMKHVVEGDYTPLSMVD
IFVKDLGLVTDTAKALKFPLPLASTAFNMFTSASNAGYGKEDDSAVIKIFSGIELPQKKGA

Sequences:

>Translated_301_residues
MNQNISVAVIGLGAMGMGAAQSCIRAGLTTYGADLNPLALEKLKAEGAKGVSQSAVDFAQELDAVLLLVVNASQVNTVLF
GEKGLASHLKPGTAVMVSSTISAQDAQQISQKLTGLGLVMLDAPVSGGAAKAAAGEMTVMASGSQQAFTKLQPVLDAVAG
KVYNIGEEIGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTHAAGNSWMFENRMKHVVEGDYTPLSMVD
IFVKDLGLVTDTAKALKFPLPLASTAFNMFTSASNAGYGKEDDSAVIKIFSGIELPQKKGA
>Mature_301_residues
MNQNISVAVIGLGAMGMGAAQSCIRAGLTTYGADLNPLALEKLKAEGAKGVSQSAVDFAQELDAVLLLVVNASQVNTVLF
GEKGLASHLKPGTAVMVSSTISAQDAQQISQKLTGLGLVMLDAPVSGGAAKAAAGEMTVMASGSQQAFTKLQPVLDAVAG
KVYNIGEEIGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTHAAGNSWMFENRMKHVVEGDYTPLSMVD
IFVKDLGLVTDTAKALKFPLPLASTAFNMFTSASNAGYGKEDDSAVIKIFSGIELPQKKGA

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=28.5223367697594, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI40556376, Length=284, Percent_Identity=21.830985915493, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789092, Length=293, Percent_Identity=77.4744027303754, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI145693186, Length=282, Percent_Identity=33.6879432624113, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790315, Length=284, Percent_Identity=29.5774647887324, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786719, Length=284, Percent_Identity=27.8169014084507, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17557316, Length=269, Percent_Identity=27.8810408921933, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24655230, Length=291, Percent_Identity=27.1477663230241, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922568, Length=291, Percent_Identity=27.1477663230241, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=25.886524822695, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30947; Mature: 30947

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNISVAVIGLGAMGMGAAQSCIRAGLTTYGADLNPLALEKLKAEGAKGVSQSAVDFAQ
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
ELDAVLLLVVNASQVNTVLFGEKGLASHLKPGTAVMVSSTISAQDAQQISQKLTGLGLVM
HHHHHHHEEECCCHHEEEEECCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEE
LDAPVSGGAAKAAAGEMTVMASGSQQAFTKLQPVLDAVAGKVYNIGEEIGLGATVKIIHQ
EECCCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHH
LLAGVHIAAGAEAMALAARAGIPLDLMYDVVTHAAGNSWMFENRMKHVVEGDYTPLSMVD
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHH
IFVKDLGLVTDTAKALKFPLPLASTAFNMFTSASNAGYGKEDDSAVIKIFSGIELPQKKG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
A
C
>Mature Secondary Structure
MNQNISVAVIGLGAMGMGAAQSCIRAGLTTYGADLNPLALEKLKAEGAKGVSQSAVDFAQ
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
ELDAVLLLVVNASQVNTVLFGEKGLASHLKPGTAVMVSSTISAQDAQQISQKLTGLGLVM
HHHHHHHEEECCCHHEEEEECCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEE
LDAPVSGGAAKAAAGEMTVMASGSQQAFTKLQPVLDAVAGKVYNIGEEIGLGATVKIIHQ
EECCCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHH
LLAGVHIAAGAEAMALAARAGIPLDLMYDVVTHAAGNSWMFENRMKHVVEGDYTPLSMVD
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHH
IFVKDLGLVTDTAKALKFPLPLASTAFNMFTSASNAGYGKEDDSAVIKIFSGIELPQKKG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]