Click here to switch to the map view.

The map label for this gene is thiD [H]

Identifier: 15603126

GI number: 15603126

Start: 1454114

End: 1454935

Strand: Reverse

Name: thiD [H]

Synonym: PM1261

Alternate gene names: 15603126

Gene position: 1454935-1454114 (Counterclockwise)

Preceding gene: 15603127

Following gene: 15603125

Centisome position: 64.45

GC content: 46.47

Gene sequence:

>822_bases
ATGAGTAACATTCCTATCGCCTTAACCATCGCTGGCTCAGATAATTGTGGTGGCGCAGGTTTCCAAGCGGATTTAAAAAC
TTTCCAGATGCAACGTGTTTTTGGCATGACCACCATTACTGCCATTCCGGTGCAAAATAGTCTTGGTGTATATGATATTC
ATCCGGTGCCATTGAATATTATTGAAGGGCAATTAAAAGCCATTGCAGACGATTACCAAGTGAATGCATTCAAAATCGGC
ATGTTAGGCAATACGGCGATTATCGAATGTATCGCTCACGCACTCAAAAAATATGATTTTGGCAGAATGGTATTGGATCC
CGTCATGTACGCCAAAGGCGGTGCTGCCTTGTTAGAACCTGATGCTGTGGAGGCGTTGCGCACCTTATTGGTGCCAAACG
CTGAAGTCATCACACCCAATTTACCCGAAGCAGAAGCACTCACAGGCATCAACATTGTCGATGATAACACCGCCTGCGAA
GCAGCAGAAAAATTACGCGCAATGGGTGTACGCCATGCGGTGATCAAGGGGGGACATAGTGAAAACTCACAAAGTGATGT
CTGTCGTGACTGGTTGTTCACCCCAACAGAAATCTTGCATTTCGACAGTCCGCGCTTTAACACGCGCCATACTCACGGGA
CAGGTTGTACCTTCTCAGCCTGTATTGCCGCGGAATTAGCCAAAGGAGCACAAGTGGTGGATGCAGTAAAAACTGCGAAA
GCCTTTATAACCGCTGCGATTAGTCATCCGCTCAATATCGGTCATGGTCATGGACCGACAAACCATTGGGCTTATGCTGA
ATGTAATCAAAAAAGCCGTTAA

Upstream 100 bases:

>100_bases
GGATTAAAAACAACACAACATGGACAATTTTATGTGGGCTTGCTGGATCAACTTGCCCACCTTTCACCTGAAACAATCAA
TCAATATGCAAGGATTTCTT

Downstream 100 bases:

>100_bases
GGAGAAAAGATGAAACCAGTCCACGCCTTTATGCGCTTATATTTCATCGCTGGTACGCAAGATTGTTTACATCTTGACGG
TGATCCTGCCCAAAATTTAC

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSNIPIALTIAGSDNCGGAGFQADLKTFQMQRVFGMTTITAIPVQNSLGVYDIHPVPLNIIEGQLKAIADDYQVNAFKIG
MLGNTAIIECIAHALKKYDFGRMVLDPVMYAKGGAALLEPDAVEALRTLLVPNAEVITPNLPEAEALTGINIVDDNTACE
AAEKLRAMGVRHAVIKGGHSENSQSDVCRDWLFTPTEILHFDSPRFNTRHTHGTGCTFSACIAAELAKGAQVVDAVKTAK
AFITAAISHPLNIGHGHGPTNHWAYAECNQKSR

Sequences:

>Translated_273_residues
MSNIPIALTIAGSDNCGGAGFQADLKTFQMQRVFGMTTITAIPVQNSLGVYDIHPVPLNIIEGQLKAIADDYQVNAFKIG
MLGNTAIIECIAHALKKYDFGRMVLDPVMYAKGGAALLEPDAVEALRTLLVPNAEVITPNLPEAEALTGINIVDDNTACE
AAEKLRAMGVRHAVIKGGHSENSQSDVCRDWLFTPTEILHFDSPRFNTRHTHGTGCTFSACIAAELAKGAQVVDAVKTAK
AFITAAISHPLNIGHGHGPTNHWAYAECNQKSR
>Mature_272_residues
SNIPIALTIAGSDNCGGAGFQADLKTFQMQRVFGMTTITAIPVQNSLGVYDIHPVPLNIIEGQLKAIADDYQVNAFKIGM
LGNTAIIECIAHALKKYDFGRMVLDPVMYAKGGAALLEPDAVEALRTLLVPNAEVITPNLPEAEALTGINIVDDNTACEA
AEKLRAMGVRHAVIKGGHSENSQSDVCRDWLFTPTEILHFDSPRFNTRHTHGTGCTFSACIAAELAKGAQVVDAVKTAKA
FITAAISHPLNIGHGHGPTNHWAYAECNQKSR

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=259, Percent_Identity=42.4710424710425, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1788758, Length=253, Percent_Identity=28.8537549407115, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325378, Length=274, Percent_Identity=30.6569343065693, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324517, Length=275, Percent_Identity=32, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=32.1167883211679, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29232; Mature: 29101

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIPIALTIAGSDNCGGAGFQADLKTFQMQRVFGMTTITAIPVQNSLGVYDIHPVPLNI
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEECCCCHHH
IEGQLKAIADDYQVNAFKIGMLGNTAIIECIAHALKKYDFGRMVLDPVMYAKGGAALLEP
HHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECC
DAVEALRTLLVPNAEVITPNLPEAEALTGINIVDDNTACEAAEKLRAMGVRHAVIKGGHS
HHHHHHHHHHCCCCEEECCCCCCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
ENSQSDVCRDWLFTPTEILHFDSPRFNTRHTHGTGCTFSACIAAELAKGAQVVDAVKTAK
CCCHHHHHHHHCCCCHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
AFITAAISHPLNIGHGHGPTNHWAYAECNQKSR
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
SNIPIALTIAGSDNCGGAGFQADLKTFQMQRVFGMTTITAIPVQNSLGVYDIHPVPLNI
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEECCCCHHH
IEGQLKAIADDYQVNAFKIGMLGNTAIIECIAHALKKYDFGRMVLDPVMYAKGGAALLEP
HHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECC
DAVEALRTLLVPNAEVITPNLPEAEALTGINIVDDNTACEAAEKLRAMGVRHAVIKGGHS
HHHHHHHHHHCCCCEEECCCCCCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
ENSQSDVCRDWLFTPTEILHFDSPRFNTRHTHGTGCTFSACIAAELAKGAQVVDAVKTAK
CCCHHHHHHHHCCCCHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHH
AFITAAISHPLNIGHGHGPTNHWAYAECNQKSR
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]