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The map label for this gene is thiM

Identifier: 15603127

GI number: 15603127

Start: 1454928

End: 1455731

Strand: Reverse

Name: thiM

Synonym: PM1262

Alternate gene names: 15603127

Gene position: 1455731-1454928 (Counterclockwise)

Preceding gene: 15603128

Following gene: 15603126

Centisome position: 64.48

GC content: 47.14

Gene sequence:

>804_bases
ATGCTAAACCCGACATACTTACAACAAATCCGCTCCCAAAATCCCCTTATCCACAATATCACTAACGTGGTAGCGGCGAA
TTTTTCCGCTAACGGACTGTTAGCGATTGGTGCCTCACCGCTGATGTCTGCGGCAATGGAAGAAATGCACGAAGTACCTC
GTCTTAGCCAAGCGCTAGTAATCAACATTGGCACCTTAATGGGCAAAGAAGTCGATGCCATGGTCTTAGCGGGTAAAACA
GCCAATGAAGTAGGTATTCCCGTGGTGCTTGATCCGGTTGGTGTTGGCGCAACCACCTTCCGCAAACAAACGGTGGCAAG
GCTACTAAAAGAAGTCAAATTTGCTGCCATTCGTGGTAATGCTGGTGAGCTAGCGACAATCGCAGAGGTGAATTGGCAAG
CGAAAGGAGTGGACGCGGGATCGGGCGAAGCCGATTTAGCACAGATTGCGCATATCGTGGCAAAAACATACCAATGCATT
GCCATGATAAGCGGCGCGACTGATTATCTTAGTGATGGCGAACACCATGCGCAAATCCATAATGGTACGCCCTTATTTCC
AAAAATCACCGCGTCAGGTTGCTTATTAAGTGCGGTGTGTGGTGCCTTTTTAGCGGTAGCGAAACCTGAACAGTATTTTG
ATGCCATGTTAGAAGCTTGTACTGCCTATGCCATTGCTGGTGAACTTGCCGCGCAAGGATTAAAAACAACACAACATGGA
CAATTTTATGTGGGCTTGCTGGATCAACTTGCCCACCTTTCACCTGAAACAATCAATCAATATGCAAGGATTTCTTATGA
GTAA

Upstream 100 bases:

>100_bases
CTTTGCCTTCTTTCTTTTCGGCAAACAAAAGAAAGAAGGTCGCCCATCGGGCGAAAGCCGATAAATAATACAAAAACCTA
ACCCAAAAGTAACAAACAAC

Downstream 100 bases:

>100_bases
CATTCCTATCGCCTTAACCATCGCTGGCTCAGATAATTGTGGTGGCGCAGGTTTCCAAGCGGATTTAAAAACTTTCCAGA
TGCAACGTGTTTTTGGCATG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MLNPTYLQQIRSQNPLIHNITNVVAANFSANGLLAIGASPLMSAAMEEMHEVPRLSQALVINIGTLMGKEVDAMVLAGKT
ANEVGIPVVLDPVGVGATTFRKQTVARLLKEVKFAAIRGNAGELATIAEVNWQAKGVDAGSGEADLAQIAHIVAKTYQCI
AMISGATDYLSDGEHHAQIHNGTPLFPKITASGCLLSAVCGAFLAVAKPEQYFDAMLEACTAYAIAGELAAQGLKTTQHG
QFYVGLLDQLAHLSPETINQYARISYE

Sequences:

>Translated_267_residues
MLNPTYLQQIRSQNPLIHNITNVVAANFSANGLLAIGASPLMSAAMEEMHEVPRLSQALVINIGTLMGKEVDAMVLAGKT
ANEVGIPVVLDPVGVGATTFRKQTVARLLKEVKFAAIRGNAGELATIAEVNWQAKGVDAGSGEADLAQIAHIVAKTYQCI
AMISGATDYLSDGEHHAQIHNGTPLFPKITASGCLLSAVCGAFLAVAKPEQYFDAMLEACTAYAIAGELAAQGLKTTQHG
QFYVGLLDQLAHLSPETINQYARISYE
>Mature_267_residues
MLNPTYLQQIRSQNPLIHNITNVVAANFSANGLLAIGASPLMSAAMEEMHEVPRLSQALVINIGTLMGKEVDAMVLAGKT
ANEVGIPVVLDPVGVGATTFRKQTVARLLKEVKFAAIRGNAGELATIAEVNWQAKGVDAGSGEADLAQIAHIVAKTYQCI
AMISGATDYLSDGEHHAQIHNGTPLFPKITASGCLLSAVCGAFLAVAKPEQYFDAMLEACTAYAIAGELAAQGLKTTQHG
QFYVGLLDQLAHLSPETINQYARISYE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=250, Percent_Identity=36.8, Blast_Score=137, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6325042, Length=273, Percent_Identity=32.967032967033, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_PASMU (P57931)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246199.1
- ProteinModelPortal:   P57931
- SMR:   P57931
- GeneID:   1244609
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1262
- NMPDR:   fig|272843.1.peg.1262
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   PMUL272843:PM1262-MONOMER
- BRENDA:   2.7.1.50
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28246; Mature: 28246

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: BINDING 42-42 BINDING 118-118 BINDING 164-164 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPTYLQQIRSQNPLIHNITNVVAANFSANGLLAIGASPLMSAAMEEMHEVPRLSQALV
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
INIGTLMGKEVDAMVLAGKTANEVGIPVVLDPVGVGATTFRKQTVARLLKEVKFAAIRGN
HHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
AGELATIAEVNWQAKGVDAGSGEADLAQIAHIVAKTYQCIAMISGATDYLSDGEHHAQIH
CCCEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHEEEC
NGTPLFPKITASGCLLSAVCGAFLAVAKPEQYFDAMLEACTAYAIAGELAAQGLKTTQHG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QFYVGLLDQLAHLSPETINQYARISYE
CHHHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLNPTYLQQIRSQNPLIHNITNVVAANFSANGLLAIGASPLMSAAMEEMHEVPRLSQALV
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
INIGTLMGKEVDAMVLAGKTANEVGIPVVLDPVGVGATTFRKQTVARLLKEVKFAAIRGN
HHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
AGELATIAEVNWQAKGVDAGSGEADLAQIAHIVAKTYQCIAMISGATDYLSDGEHHAQIH
CCCEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHEEEC
NGTPLFPKITASGCLLSAVCGAFLAVAKPEQYFDAMLEACTAYAIAGELAAQGLKTTQHG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QFYVGLLDQLAHLSPETINQYARISYE
CHHHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100