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The map label for this gene is dlgD

Identifier: 15603121

GI number: 15603121

Start: 1449321

End: 1450319

Strand: Reverse

Name: dlgD

Synonym: PM1256

Alternate gene names: 15603121

Gene position: 1450319-1449321 (Counterclockwise)

Preceding gene: 15603123

Following gene: 15603120

Centisome position: 64.24

GC content: 44.94

Gene sequence:

>999_bases
ATGAAAGTCAGTTACGAAGCCCTTAAACAAGAATTTAAACGCGTATTACTCGCTCGAAATGTCCGTGAAGACATTGCGGA
AGAATGTGCAACTATGTTTGCTGATACCACGGAGTCTGGTGTGTATTCACATGGTGTAAACCGCTTTCCACGTTTTATTT
CTCAACTTGAAAAAGGCGATATTGTACCCGATGCAGAACCCACGAAAGTGCTGTCTTTAGGCGCAATTGAACAATGGGAT
GCGCATCAAGCAATTGGTAACTTGACAGCCAAAAAAATGATGGATCGTGCTATGGAAATTGCGGACCAATTTGGTATCGG
TGTGGTCGCACTGAAAAACGCTAACCATTGGATGCGTGGTGGTGGATACGGCTGGCAAGCCGCCGAAAAAGGCTATATCG
GCATCTGTTGGACAAACTCCATCGCAGTTATGCCACCTTGGGGCGCAAAAGAATGCCGTATTGGAACAAACCCCTTGATT
ATTGCGGTGCCGACAACCCCGATTACAATGGTCGATATGTCTTGCTCTATGTATTCCTATGGCATGTTAGAAGTACACCG
TTTAGCAGGTCGCCAAACCTTTGTTGATGCCGGTTTTGATGATGAAGGGAACTTAACGCGCGATCCGGGCACCGTCGAGA
AAAATCGTCGTTTACTTCCAATGGGCTTCTGGAAAGGCTCCGGATTATCCATTGTACTCGATATGATCGCGACTTTACTT
TCTAATGGCTTGTCTGTTGCGGAAGTGACCGAAGAAAAAGACGATGAATACTGTGTCTCACAAATCTTTATTGCGATTGA
AGTTGATCGCCTCATTGACGGTAACACCAAAGACGAAAAACTAAACAAAATTATGGATTACGTCAGAACCGCTGAACGCG
CCGATCCCGATGTAGCTATTCGCTTACCGGGTCATGAATTCACAGCGATTCGGGCTGAAAACAAAGCCAATGGTATCCCT
GTTGATGAAACGGTATGGGAAAAAATTAAATCGTTATAA

Upstream 100 bases:

>100_bases
TTATTCTGCTTTTTAATTTGCTAACCCGCATAAATCCGATTATAATTTTTTAAAACGTCGTTTCAATATGTGAAATAAAA
CACTGAATTGTGAGGATAAT

Downstream 100 bases:

>100_bases
GGAAAGAATATGTTTTTTGGTCATCTCTCTCAGGTTGACAAAAAGCAGTATCCTGAAGCAATCAATATTGCTTTAGATTA
TTTGTCAAACACTGACTTTA

Product: 2,3-diketo-L-gulonate reductase

Products: NA

Alternate protein names: 2,3-DKG reductase; 3-dehydro-L-gulonate 2-dehydrogenase

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKVSYEALKQEFKRVLLARNVREDIAEECATMFADTTESGVYSHGVNRFPRFISQLEKGDIVPDAEPTKVLSLGAIEQWD
AHQAIGNLTAKKMMDRAMEIADQFGIGVVALKNANHWMRGGGYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
IAVPTTPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDEGNLTRDPGTVEKNRRLLPMGFWKGSGLSIVLDMIATLL
SNGLSVAEVTEEKDDEYCVSQIFIAIEVDRLIDGNTKDEKLNKIMDYVRTAERADPDVAIRLPGHEFTAIRAENKANGIP
VDETVWEKIKSL

Sequences:

>Translated_332_residues
MKVSYEALKQEFKRVLLARNVREDIAEECATMFADTTESGVYSHGVNRFPRFISQLEKGDIVPDAEPTKVLSLGAIEQWD
AHQAIGNLTAKKMMDRAMEIADQFGIGVVALKNANHWMRGGGYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
IAVPTTPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDEGNLTRDPGTVEKNRRLLPMGFWKGSGLSIVLDMIATLL
SNGLSVAEVTEEKDDEYCVSQIFIAIEVDRLIDGNTKDEKLNKIMDYVRTAERADPDVAIRLPGHEFTAIRAENKANGIP
VDETVWEKIKSL
>Mature_332_residues
MKVSYEALKQEFKRVLLARNVREDIAEECATMFADTTESGVYSHGVNRFPRFISQLEKGDIVPDAEPTKVLSLGAIEQWD
AHQAIGNLTAKKMMDRAMEIADQFGIGVVALKNANHWMRGGGYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
IAVPTTPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDEGNLTRDPGTVEKNRRLLPMGFWKGSGLSIVLDMIATLL
SNGLSVAEVTEEKDDEYCVSQIFIAIEVDRLIDGNTKDEKLNKIMDYVRTAERADPDVAIRLPGHEFTAIRAENKANGIP
VDETVWEKIKSL

Specific function: Catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH, to form 3-keto-L-gulonate

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family. DlgD subfamily

Homologues:

Organism=Escherichia coli, GI1790000, Length=332, Percent_Identity=75.3012048192771, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1787020, Length=301, Percent_Identity=26.2458471760797, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786727, Length=338, Percent_Identity=24.8520710059172, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17507179, Length=317, Percent_Identity=25.5520504731861, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17536633, Length=311, Percent_Identity=27.0096463022508, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45553211, Length=300, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24667912, Length=300, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24667908, Length=300, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24667904, Length=300, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24658010, Length=267, Percent_Identity=26.5917602996255, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648360, Length=304, Percent_Identity=25.6578947368421, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DLGD_PASMU (Q9CLH5)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246193.1
- ProteinModelPortal:   Q9CLH5
- SMR:   Q9CLH5
- GeneID:   1244603
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1256
- NMPDR:   fig|272843.1.peg.1256
- HOGENOM:   HBG293006
- OMA:   TNALAVM
- ProtClustDB:   PRK13260
- BioCyc:   PMUL272843:PM1256-MONOMER
- BRENDA:   1.1.1.130
- GO:   GO:0005737
- HAMAP:   MF_00820
- InterPro:   IPR003767
- PANTHER:   PTHR11091

Pfam domain/function: PF02615 Ldh_2; SSF89733 MDH_LDH

EC number: =1.1.1.130

Molecular weight: Translated: 36869; Mature: 36869

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: ACT_SITE 44-44

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSYEALKQEFKRVLLARNVREDIAEECATMFADTTESGVYSHGVNRFPRFISQLEKGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
IVPDAEPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMEIADQFGIGVVALKNANHWMRG
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECC
GGYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLIIAVPTTPITMVDMSCSMYSY
CCCCCCCCCCCEEEEEEECCEEEECCCCCCCCEECCCCEEEEECCCCEEEEECCCCHHHH
GMLEVHRLAGRQTFVDAGFDDEGNLTRDPGTVEKNRRLLPMGFWKGSGLSIVLDMIATLL
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
SNGLSVAEVTEEKDDEYCVSQIFIAIEVDRLIDGNTKDEKLNKIMDYVRTAERADPDVAI
HCCCEEEHHHHCCCHHHHHHHHEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEE
RLPGHEFTAIRAENKANGIPVDETVWEKIKSL
EECCCCEEEEEECCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKVSYEALKQEFKRVLLARNVREDIAEECATMFADTTESGVYSHGVNRFPRFISQLEKGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
IVPDAEPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMEIADQFGIGVVALKNANHWMRG
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECC
GGYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLIIAVPTTPITMVDMSCSMYSY
CCCCCCCCCCCEEEEEEECCEEEECCCCCCCCEECCCCEEEEECCCCEEEEECCCCHHHH
GMLEVHRLAGRQTFVDAGFDDEGNLTRDPGTVEKNRRLLPMGFWKGSGLSIVLDMIATLL
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
SNGLSVAEVTEEKDDEYCVSQIFIAIEVDRLIDGNTKDEKLNKIMDYVRTAERADPDVAI
HCCCEEEHHHHCCCHHHHHHHHEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEE
RLPGHEFTAIRAENKANGIPVDETVWEKIKSL
EECCCCEEEEEECCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100