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The map label for this gene is rffG [H]

Identifier: 15602895

GI number: 15602895

Start: 1212437

End: 1213474

Strand: Reverse

Name: rffG [H]

Synonym: PM1030

Alternate gene names: 15602895

Gene position: 1213474-1212437 (Counterclockwise)

Preceding gene: 15602897

Following gene: 15602894

Centisome position: 53.75

GC content: 42.29

Gene sequence:

>1038_bases
ATGAAAAAGATCCTTATTACTGGTGGGGCAGGTTTTATTGGCTCCGCCGTTATTCGCTACATTTTAAGCCAAACATCAGA
TCAGATTATAAATCTCGATAAACTCACCTATGCCAGTAATTTGGACGCTTTAGCCACTGTTGTTGACAACCCACGTTACC
ATTTTGAACAAGGTGACATTGCAGATGCCACGACCTTGAGCAACATTTTTCAACGCTATCAACCGGATTGTGTTATGCAT
TTGGCAGCAGAAAGTCATGTTGATCGCTCAATTGCTGGCGCAGCCGCGTTTATTCAAACAAATATTGTTGGTACCTATAC
TTTACTTGAGGTGGCTCGGCAATATTGGCTCACATTAGACGAAAAAAGCAAAGCGGCTTTTCGTTTTCACCATGTTTCAA
CGGATGAGGTATTTGGTGACCTTGACTTCACACAACCTGCGTTTACAGAAACTTCCCCCTATCGTCCAAGTAGCCCTTAT
TCTGCCTCAAAAGCAGCTAGTGATCATTTAGTACGAGCTTGGCATCGAACTTATGGTTTGCCCATTGTGATGAGCAACAG
TACGAATAATTATGGTTTTTATCAACATACTGAAAAACTCATTCCTTTTATGTTAAGTCAGGCTTTACAGGGAAAACCGT
TGACGCTCTATGGCGATGGGCAGCAAGTGCGAGATTGGTTGTTTGTTGATGATCATGCACAAGCTTTGTATCTTGTACTA
ACAAAGGGCAAAGTGGGGGAAACCTATAATATTGGTGCACGTTGTGAGTACAGTAATATTCATCTTGTGATGCGGTTATG
TGAGATCTTAAATCAGTTAAGACAGGCGGGCGGATTGACAGCGTTTTCGGATCAACTCAGCGAAATAACCGACTTTAAGC
AGCTGATCACTTTTGTGAAAGATCGTCCCGGTCATGACTTGCGTTATGCGATAAATGCCTCAAAAATTCAAGACGAATTA
GGCTGGGTGGCAAAAACGGATTTTGAGACGGGATTACGAAAAACAGTCGCATGGTATGTGGATTATTTGTCACCGTGA

Upstream 100 bases:

>100_bases
GCATTTTGATTTTAATTGCTGTATATTATACGGTTGTGCTGAATTATACAAGTTTTGTGATGTACGGCAAGTTTTTATTC
CATCCGTTTCAAGATAAAAG

Downstream 100 bases:

>100_bases
AAGAAAAGATATTTTGTGGAAAAGGTACTAAAGTGCGGAAAAACTGGTATAGTGAATCGCACTTTATTTTTATTTAGATA
ATACAAAAAATAAAAGGATC

Product: RffG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKKILITGGAGFIGSAVIRYILSQTSDQIINLDKLTYASNLDALATVVDNPRYHFEQGDIADATTLSNIFQRYQPDCVMH
LAAESHVDRSIAGAAAFIQTNIVGTYTLLEVARQYWLTLDEKSKAAFRFHHVSTDEVFGDLDFTQPAFTETSPYRPSSPY
SASKAASDHLVRAWHRTYGLPIVMSNSTNNYGFYQHTEKLIPFMLSQALQGKPLTLYGDGQQVRDWLFVDDHAQALYLVL
TKGKVGETYNIGARCEYSNIHLVMRLCEILNQLRQAGGLTAFSDQLSEITDFKQLITFVKDRPGHDLRYAINASKIQDEL
GWVAKTDFETGLRKTVAWYVDYLSP

Sequences:

>Translated_345_residues
MKKILITGGAGFIGSAVIRYILSQTSDQIINLDKLTYASNLDALATVVDNPRYHFEQGDIADATTLSNIFQRYQPDCVMH
LAAESHVDRSIAGAAAFIQTNIVGTYTLLEVARQYWLTLDEKSKAAFRFHHVSTDEVFGDLDFTQPAFTETSPYRPSSPY
SASKAASDHLVRAWHRTYGLPIVMSNSTNNYGFYQHTEKLIPFMLSQALQGKPLTLYGDGQQVRDWLFVDDHAQALYLVL
TKGKVGETYNIGARCEYSNIHLVMRLCEILNQLRQAGGLTAFSDQLSEITDFKQLITFVKDRPGHDLRYAINASKIQDEL
GWVAKTDFETGLRKTVAWYVDYLSP
>Mature_345_residues
MKKILITGGAGFIGSAVIRYILSQTSDQIINLDKLTYASNLDALATVVDNPRYHFEQGDIADATTLSNIFQRYQPDCVMH
LAAESHVDRSIAGAAAFIQTNIVGTYTLLEVARQYWLTLDEKSKAAFRFHHVSTDEVFGDLDFTQPAFTETSPYRPSSPY
SASKAASDHLVRAWHRTYGLPIVMSNSTNNYGFYQHTEKLIPFMLSQALQGKPLTLYGDGQQVRDWLFVDDHAQALYLVL
TKGKVGETYNIGARCEYSNIHLVMRLCEILNQLRQAGGLTAFSDQLSEITDFKQLITFVKDRPGHDLRYAINASKIQDEL
GWVAKTDFETGLRKTVAWYVDYLSP

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=340, Percent_Identity=36.1764705882353, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI42516563, Length=343, Percent_Identity=23.3236151603499, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4504031, Length=275, Percent_Identity=24.7272727272727, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI48994969, Length=340, Percent_Identity=62.0588235294118, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1788353, Length=346, Percent_Identity=57.2254335260116, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1788366, Length=289, Percent_Identity=26.9896193771626, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=344, Percent_Identity=37.5, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=341, Percent_Identity=35.7771260997067, Blast_Score=184, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17539532, Length=343, Percent_Identity=24.4897959183673, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=359, Percent_Identity=24.5125348189415, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71982038, Length=363, Percent_Identity=24.7933884297521, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=359, Percent_Identity=23.3983286908078, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=359, Percent_Identity=23.3983286908078, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=359, Percent_Identity=23.3983286908078, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=359, Percent_Identity=23.3983286908078, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=359, Percent_Identity=23.3983286908078, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=359, Percent_Identity=22.5626740947075, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=262, Percent_Identity=26.7175572519084, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21356223, Length=348, Percent_Identity=22.9885057471264, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19923002, Length=366, Percent_Identity=24.8633879781421, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38802; Mature: 38802

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILITGGAGFIGSAVIRYILSQTSDQIINLDKLTYASNLDALATVVDNPRYHFEQGDI
CCEEEEECCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCHHHHHHHCCCCEEECCCCC
ADATTLSNIFQRYQPDCVMHLAAESHVDRSIAGAAAFIQTNIVGTYTLLEVARQYWLTLD
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEECC
EKSKAAFRFHHVSTDEVFGDLDFTQPAFTETSPYRPSSPYSASKAASDHLVRAWHRTYGL
CCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PIVMSNSTNNYGFYQHTEKLIPFMLSQALQGKPLTLYGDGQQVRDWLFVDDHAQALYLVL
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHEEEECCCCCEEEEEE
TKGKVGETYNIGARCEYSNIHLVMRLCEILNQLRQAGGLTAFSDQLSEITDFKQLITFVK
ECCCCCCEECCCCEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
DRPGHDLRYAINASKIQDELGWVAKTDFETGLRKTVAWYVDYLSP
CCCCCCEEEEECHHHHHHHHCCEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKILITGGAGFIGSAVIRYILSQTSDQIINLDKLTYASNLDALATVVDNPRYHFEQGDI
CCEEEEECCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCHHHHHHHCCCCEEECCCCC
ADATTLSNIFQRYQPDCVMHLAAESHVDRSIAGAAAFIQTNIVGTYTLLEVARQYWLTLD
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEECC
EKSKAAFRFHHVSTDEVFGDLDFTQPAFTETSPYRPSSPYSASKAASDHLVRAWHRTYGL
CCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PIVMSNSTNNYGFYQHTEKLIPFMLSQALQGKPLTLYGDGQQVRDWLFVDDHAQALYLVL
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHEEEECCCCCEEEEEE
TKGKVGETYNIGARCEYSNIHLVMRLCEILNQLRQAGGLTAFSDQLSEITDFKQLITFVK
ECCCCCCEECCCCEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
DRPGHDLRYAINASKIQDELGWVAKTDFETGLRKTVAWYVDYLSP
CCCCCCEEEEECHHHHHHHHCCEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]