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The map label for this gene is folE

Identifier: 15602558

GI number: 15602558

Start: 804251

End: 804907

Strand: Reverse

Name: folE

Synonym: PM0693

Alternate gene names: 15602558

Gene position: 804907-804251 (Counterclockwise)

Preceding gene: 15602565

Following gene: 15602556

Centisome position: 35.66

GC content: 40.49

Gene sequence:

>657_bases
ATGAACAAGATTTCACCAGAAGCAGAAAAAGTCCGCAACGCATTATTAAGTAAGGGGATTGAAACCCCAATGATTACCCC
AGAACAAGACAGAGATACGCGTCGAGCGGGGATTGAACAACATATGCGTGAAGTGATGAAGCTGATTGGGTTAGATTTAC
GTGATGATAGTTTAGAAGAAACCCCCGTGCGTTTGGCGAAAATGTTTGTTGATGAAATTTTCAGCGGATTAGACTATGCG
AATTTCCCGAAAATTACTAACATTGAAAATCGTATGAAAGTCAGTGAAATGGTGTTGGTGAATGACGTGACGTTAACCAG
TACTTGTGAACACCATTTTGTGACTATTGATGGGATGGTTTCGGTGGCGTATTACCCGAAAAAATGGGTCATTGGGTTGT
CTAAAATTAATCGCATCGTGGCGTTTTTTGCACAACGTCCACAAGTACAAGAGCGTTTGACTGAACAAATTTTGTTGGCA
TTCCAAACGATTTTAGAAACGGACGATGTGGCGGTGTATGTAAAGGCTACTCATTTCTGCGTGAAATGCCGTGGCATTAA
AGATACCAACAGTTATACGGTGACATCCGCATTTGGTGGTGTCTTTTTAGATGACCGTGAAACGCGTAAAGAGTTTTTAA
CCTTAATTAATAAATAA

Upstream 100 bases:

>100_bases
ACCATATTTCTAAAACTTTTCATCCTCTCTACCAATTATTCTCATTTGTACTTTGGCGAGAGTTTGCTAGAATGTGCGGA
TTATTTATCGGGAGTTTTAC

Downstream 100 bases:

>100_bases
GTGCGTTAAAACTTTCTAGAAACCGACCGCACTTTGGGCGTCAATATAACTAAAGCACCTTGAGTCAGAACAAGGTGCTT
TTTGTTTGTTCGGCTTAAAG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MNKISPEAEKVRNALLSKGIETPMITPEQDRDTRRAGIEQHMREVMKLIGLDLRDDSLEETPVRLAKMFVDEIFSGLDYA
NFPKITNIENRMKVSEMVLVNDVTLTSTCEHHFVTIDGMVSVAYYPKKWVIGLSKINRIVAFFAQRPQVQERLTEQILLA
FQTILETDDVAVYVKATHFCVKCRGIKDTNSYTVTSAFGGVFLDDRETRKEFLTLINK

Sequences:

>Translated_218_residues
MNKISPEAEKVRNALLSKGIETPMITPEQDRDTRRAGIEQHMREVMKLIGLDLRDDSLEETPVRLAKMFVDEIFSGLDYA
NFPKITNIENRMKVSEMVLVNDVTLTSTCEHHFVTIDGMVSVAYYPKKWVIGLSKINRIVAFFAQRPQVQERLTEQILLA
FQTILETDDVAVYVKATHFCVKCRGIKDTNSYTVTSAFGGVFLDDRETRKEFLTLINK
>Mature_218_residues
MNKISPEAEKVRNALLSKGIETPMITPEQDRDTRRAGIEQHMREVMKLIGLDLRDDSLEETPVRLAKMFVDEIFSGLDYA
NFPKITNIENRMKVSEMVLVNDVTLTSTCEHHFVTIDGMVSVAYYPKKWVIGLSKINRIVAFFAQRPQVQERLTEQILLA
FQTILETDDVAVYVKATHFCVKCRGIKDTNSYTVTSAFGGVFLDDRETRKEFLTLINK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=218, Percent_Identity=38.5321100917431, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI4503949, Length=218, Percent_Identity=38.5321100917431, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI66932970, Length=179, Percent_Identity=34.6368715083799, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI66932972, Length=179, Percent_Identity=34.6368715083799, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788476, Length=215, Percent_Identity=64.6511627906977, Blast_Score=280, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI17560486, Length=183, Percent_Identity=40.4371584699454, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321706, Length=183, Percent_Identity=40.4371584699454, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24656791, Length=122, Percent_Identity=50, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24656782, Length=122, Percent_Identity=50, Blast_Score=116, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24656796, Length=122, Percent_Identity=50, Blast_Score=116, Evalue=9e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_PASMU (P57865)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245630.1
- ProteinModelPortal:   P57865
- SMR:   P57865
- GeneID:   1244040
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0693
- NMPDR:   fig|272843.1.peg.693
- HOGENOM:   HBG370191
- OMA:   ACSHHLC
- ProtClustDB:   PRK09347
- BioCyc:   PMUL272843:PM0693-MONOMER
- BRENDA:   3.5.4.16
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 24953; Mature: 24953

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKISPEAEKVRNALLSKGIETPMITPEQDRDTRRAGIEQHMREVMKLIGLDLRDDSLEE
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
TPVRLAKMFVDEIFSGLDYANFPKITNIENRMKVSEMVLVNDVTLTSTCEHHFVTIDGMV
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEECCEEECCCCCEEEEECCEE
SVAYYPKKWVIGLSKINRIVAFFAQRPQVQERLTEQILLAFQTILETDDVAVYVKATHFC
EEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE
VKCRGIKDTNSYTVTSAFGGVFLDDRETRKEFLTLINK
EEECCCCCCCCEEEEECCCCEEECCHHHHHHHHHHHCC
>Mature Secondary Structure
MNKISPEAEKVRNALLSKGIETPMITPEQDRDTRRAGIEQHMREVMKLIGLDLRDDSLEE
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
TPVRLAKMFVDEIFSGLDYANFPKITNIENRMKVSEMVLVNDVTLTSTCEHHFVTIDGMV
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEECCEEECCCCCEEEEECCEE
SVAYYPKKWVIGLSKINRIVAFFAQRPQVQERLTEQILLAFQTILETDDVAVYVKATHFC
EEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE
VKCRGIKDTNSYTVTSAFGGVFLDDRETRKEFLTLINK
EEECCCCCCCCEEEEECCCCEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100