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The map label for this gene is xthA [H]

Identifier: 15602556

GI number: 15602556

Start: 801144

End: 801947

Strand: Reverse

Name: xthA [H]

Synonym: PM0691

Alternate gene names: 15602556

Gene position: 801947-801144 (Counterclockwise)

Preceding gene: 15602558

Following gene: 15602555

Centisome position: 35.52

GC content: 39.55

Gene sequence:

>804_bases
ATGAAAGTCATTTCTTTTAATATTAATGGATTGAGAGCAAGACCGCATCAATTAGAAGCGGTGATTGAGAAATACCAACC
CGATGTGTTGGGATTGCAAGAGATTAAGGTAGCAGATGAAATGTTCCCTTATGAACTGGTTGAACATTTGGGCTACCATG
TTTTTCATCATGGTCAAAAAGGACACTACGGTGTTGCGTTGTTAACCAAGCAAGCGCCTAAAGCGGTACGTAAAGGGTTT
CCAACAGACAGCGAGGAAGCTCAAAAACGTATTATTATGGCGGATTTAGAAACAGATTTTGGCTTATTAACAGTGATTAA
TGGTTATTTTCCACAAGGCGAAAGTCGCGATCATGAAACTAAATTCCCCGCTAAAGAAAAATTTTATGCGGATTTACAAC
AGTATTTAGAACAGGATCACAATGCCAATAATCCGATTATTATTATGGGCGACATGAATATTAGTCCGACAGATCTGGAT
ATTGGGATTGGTGATGAAAATCGTAAACGTTGGTTACGCACGGGCAAATGTTCGTTTTTACCGGAAGAAAGAGAATGGAT
GAACCGTTTGTATGGCTACGGTTTAGTGGACACTTTCCGTCATTTAAATCCGGATGTGAACGATCAATTTTCGTGGTTTG
ATTATCGCTCAAAAGGGTTTGATGATAATCGTGGTTTACGTATTGATCATATTATTGCAAACCATGCTCTAGCAGCACGC
TGTGTTGAAACAGGGATTGCGTTGGATATCCGTGCAATGGAAAAACCTTCTGATCATGCACCGGTTTGGGCAGTATTTAA
ATAA

Upstream 100 bases:

>100_bases
AAGCTAAGGGGCAATGACAGAATGTTTTTATTGCTTTGGCAATACACGAGATCACAGTATAATGCTGACTTTATCTAATG
ATGAATTAGGAAATTTAGGC

Downstream 100 bases:

>100_bases
GGTACTCGCATGGATAAAGGACAAAGCTGGCAAAATTATCGTTCTGTACTGAATGACAAACTGGCGATTTTTTCAGTGAA
TCTTGAGGTATTACATCAAT

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKVISFNINGLRARPHQLEAVIEKYQPDVLGLQEIKVADEMFPYELVEHLGYHVFHHGQKGHYGVALLTKQAPKAVRKGF
PTDSEEAQKRIIMADLETDFGLLTVINGYFPQGESRDHETKFPAKEKFYADLQQYLEQDHNANNPIIIMGDMNISPTDLD
IGIGDENRKRWLRTGKCSFLPEEREWMNRLYGYGLVDTFRHLNPDVNDQFSWFDYRSKGFDDNRGLRIDHIIANHALAAR
CVETGIALDIRAMEKPSDHAPVWAVFK

Sequences:

>Translated_267_residues
MKVISFNINGLRARPHQLEAVIEKYQPDVLGLQEIKVADEMFPYELVEHLGYHVFHHGQKGHYGVALLTKQAPKAVRKGF
PTDSEEAQKRIIMADLETDFGLLTVINGYFPQGESRDHETKFPAKEKFYADLQQYLEQDHNANNPIIIMGDMNISPTDLD
IGIGDENRKRWLRTGKCSFLPEEREWMNRLYGYGLVDTFRHLNPDVNDQFSWFDYRSKGFDDNRGLRIDHIIANHALAAR
CVETGIALDIRAMEKPSDHAPVWAVFK
>Mature_267_residues
MKVISFNINGLRARPHQLEAVIEKYQPDVLGLQEIKVADEMFPYELVEHLGYHVFHHGQKGHYGVALLTKQAPKAVRKGF
PTDSEEAQKRIIMADLETDFGLLTVINGYFPQGESRDHETKFPAKEKFYADLQQYLEQDHNANNPIIIMGDMNISPTDLD
IGIGDENRKRWLRTGKCSFLPEEREWMNRLYGYGLVDTFRHLNPDVNDQFSWFDYRSKGFDDNRGLRIDHIIANHALAAR
CVETGIALDIRAMEKPSDHAPVWAVFK

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=269, Percent_Identity=26.0223048327138, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI18375503, Length=269, Percent_Identity=26.0223048327138, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI18375501, Length=269, Percent_Identity=26.0223048327138, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=74.1573033707865, Blast_Score=428, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71989536, Length=269, Percent_Identity=25.6505576208178, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI221330655, Length=269, Percent_Identity=31.5985130111524, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17136678, Length=269, Percent_Identity=31.5985130111524, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30756; Mature: 30756

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVISFNINGLRARPHQLEAVIEKYQPDVLGLQEIKVADEMFPYELVEHLGYHVFHHGQK
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHCCHHEEECCCC
GHYGVALLTKQAPKAVRKGFPTDSEEAQKRIIMADLETDFGLLTVINGYFPQGESRDHET
CCEEEEEEECHHHHHHHCCCCCCHHHHHHEEEEEECCCCCCCEEHHHCCCCCCCCCCCCC
KFPAKEKFYADLQQYLEQDHNANNPIIIMGDMNISPTDLDIGIGDENRKRWLRTGKCSFL
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHCCCCCCC
PEEREWMNRLYGYGLVDTFRHLNPDVNDQFSWFDYRSKGFDDNRGLRIDHIIANHALAAR
CHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEHHCCCCCCCCCCEEHHHHHHHHHHHH
CVETGIALDIRAMEKPSDHAPVWAVFK
HHHCCCEEEEEECCCCCCCCCEEEEEC
>Mature Secondary Structure
MKVISFNINGLRARPHQLEAVIEKYQPDVLGLQEIKVADEMFPYELVEHLGYHVFHHGQK
CEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHCCHHEEECCCC
GHYGVALLTKQAPKAVRKGFPTDSEEAQKRIIMADLETDFGLLTVINGYFPQGESRDHET
CCEEEEEEECHHHHHHHCCCCCCHHHHHHEEEEEECCCCCCCEEHHHCCCCCCCCCCCCC
KFPAKEKFYADLQQYLEQDHNANNPIIIMGDMNISPTDLDIGIGDENRKRWLRTGKCSFL
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHCCCCCCC
PEEREWMNRLYGYGLVDTFRHLNPDVNDQFSWFDYRSKGFDDNRGLRIDHIIANHALAAR
CHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEHHCCCCCCCCCCEEHHHHHHHHHHHH
CVETGIALDIRAMEKPSDHAPVWAVFK
HHHCCCEEEEEECCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]