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The map label for this gene is rluB

Identifier: 15602526

GI number: 15602526

Start: 768603

End: 769640

Strand: Reverse

Name: rluB

Synonym: PM0661

Alternate gene names: 15602526

Gene position: 769640-768603 (Counterclockwise)

Preceding gene: 15602527

Following gene: 15602525

Centisome position: 34.09

GC content: 42.49

Gene sequence:

>1038_bases
ATGAAGTTTACCAAACCAAATTCTCGTCAAAATAAACCGCACGTTGAGCAAAAAACATCGCGACGTTCTACAAACGCGAA
TGCTGTGGCGAGTAAAAATGTGAAAAAGGTGACAGAAGCACAACCGAAATCGGTAAAAGCCAGTACCCATAGTGTAAAAC
CCACAGTTAAGAACCCCTTAGTTGAAGGGGAAAAACTACAGAAAGTGTTAGCGCGAGCCGGTCAAGGTTCACGTCGTGAA
ATAGAAGCGATGATTGCCGAAAATCGTGTCAGCGTTGATGGAAAGATCGCCACTCTAGGTGATCGTATTGATGTACACGC
TGGCGTGAAAATCCGTATTGATGGACATCTTATTAATTTACTTCATGCACAAAAAGAAGTGTGTCGTGTACTGATGTATT
ACAAACCTGAAGGGGAGTTATGTACACGTCATGATCCTGAAGGAAGACCGACCGTATTTGACCGTTTACCACGTTTAACC
GGTTCTCGTTGGATTGCTGTCGGACGCTTGGATATTAATACTTCAGGATTGTTGCTTTTCACGACCGATGGCGAATTAGC
GAACCGTTTAATGCATCCAAGTCGAGAAGTAGAGCGTGAATATTCTGTACGTGTTTTCGGGCAAGTTGATGAAGCGATGC
TACATCGCTTAAAAAAAGGTGTTCAATTAGAAGACGGTCCTGCCAATTTTAAAGAGATTAAATTTGCTGGTGGTGTGGGC
ATGAACCAATGGTTCGACGTGACTTTAATGGAGGGACGTAATCGCGAAGTTCGTCGTCTATGGGAGTCCCAAGGTGTACA
AGTGAGCAGATTAATTCGTACTCGATATGGCAATATTTCTTTAATGAAATCGTTACCTCGAGGCGGTTGGGAAGAAATGG
ATTTAACCAATGTGAATTATCTAAGGGAATTAGTTGGGTTACCAGCAGAGGTGGAAACGAAACTTGACGTGACAAAACCT
CGCCGTAGAGCGAAAACAGGTCAAATTCGTAAGGCTGTGAAGCGTTATGCTGAATTAAATAAACGTTATAAAAAATAA

Upstream 100 bases:

>100_bases
TACCCCGATAATTATCCGCGAAGGGAGTGGGTCTACCGCGCCTTTTGTCTAATATCATGTCGTAAAAAGACGCTTGTGAA
AGCGACAAATTAGAGGAAAT

Downstream 100 bases:

>100_bases
GTTTTTTCTTATACAGCCAGTGCTGTGAGAGGATAACTGAATGAAAATACAACAATTACGTTATATTGTAGAAATTGTTA
ATCAAAATTTAAATGTAACA

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKFTKPNSRQNKPHVEQKTSRRSTNANAVASKNVKKVTEAQPKSVKASTHSVKPTVKNPLVEGEKLQKVLARAGQGSRRE
IEAMIAENRVSVDGKIATLGDRIDVHAGVKIRIDGHLINLLHAQKEVCRVLMYYKPEGELCTRHDPEGRPTVFDRLPRLT
GSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDEAMLHRLKKGVQLEDGPANFKEIKFAGGVG
MNQWFDVTLMEGRNREVRRLWESQGVQVSRLIRTRYGNISLMKSLPRGGWEEMDLTNVNYLRELVGLPAEVETKLDVTKP
RRRAKTGQIRKAVKRYAELNKRYKK

Sequences:

>Translated_345_residues
MKFTKPNSRQNKPHVEQKTSRRSTNANAVASKNVKKVTEAQPKSVKASTHSVKPTVKNPLVEGEKLQKVLARAGQGSRRE
IEAMIAENRVSVDGKIATLGDRIDVHAGVKIRIDGHLINLLHAQKEVCRVLMYYKPEGELCTRHDPEGRPTVFDRLPRLT
GSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDEAMLHRLKKGVQLEDGPANFKEIKFAGGVG
MNQWFDVTLMEGRNREVRRLWESQGVQVSRLIRTRYGNISLMKSLPRGGWEEMDLTNVNYLRELVGLPAEVETKLDVTKP
RRRAKTGQIRKAVKRYAELNKRYKK
>Mature_345_residues
MKFTKPNSRQNKPHVEQKTSRRSTNANAVASKNVKKVTEAQPKSVKASTHSVKPTVKNPLVEGEKLQKVLARAGQGSRRE
IEAMIAENRVSVDGKIATLGDRIDVHAGVKIRIDGHLINLLHAQKEVCRVLMYYKPEGELCTRHDPEGRPTVFDRLPRLT
GSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGQVDEAMLHRLKKGVQLEDGPANFKEIKFAGGVG
MNQWFDVTLMEGRNREVRRLWESQGVQVSRLIRTRYGNISLMKSLPRGGWEEMDLTNVNYLRELVGLPAEVETKLDVTKP
RRRAKTGQIRKAVKRYAELNKRYKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787524, Length=277, Percent_Identity=73.6462093862816, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=31.3304721030043, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=31.2236286919831, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081838, Length=185, Percent_Identity=32.4324324324324, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUB_PASMU (Q9CMY9)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245598.1
- ProteinModelPortal:   Q9CMY9
- SMR:   Q9CMY9
- GeneID:   1244008
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0661
- NMPDR:   fig|272843.1.peg.661
- HOGENOM:   HBG754478
- OMA:   ELCTRSD
- ProtClustDB:   CLSK870190
- BioCyc:   PMUL272843:PM0661-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 39236; Mature: 39236

Theoretical pI: Translated: 10.87; Mature: 10.87

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTKPNSRQNKPHVEQKTSRRSTNANAVASKNVKKVTEAQPKSVKASTHSVKPTVKNPL
CCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCHHCCC
VEGEKLQKVLARAGQGSRREIEAMIAENRVSVDGKIATLGDRIDVHAGVKIRIDGHLINL
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEECCCEEEEECCEEEEECCHHHHH
LHAQKEVCRVLMYYKPEGELCTRHDPEGRPTVFDRLPRLTGSRWIAVGRLDINTSGLLLF
HHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCEEEE
TTDGELANRLMHPSREVEREYSVRVFGQVDEAMLHRLKKGVQLEDGPANFKEIKFAGGVG
ECCHHHHHHHCCCCHHHHHHHCEEEECCHHHHHHHHHHCCCEECCCCCCHHEEEEECCCC
MNQWFDVTLMEGRNREVRRLWESQGVQVSRLIRTRYGNISLMKSLPRGGWEEMDLTNVNY
CCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHH
LRELVGLPAEVETKLDVTKPRRRAKTGQIRKAVKRYAELNKRYKK
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFTKPNSRQNKPHVEQKTSRRSTNANAVASKNVKKVTEAQPKSVKASTHSVKPTVKNPL
CCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCHHCCC
VEGEKLQKVLARAGQGSRREIEAMIAENRVSVDGKIATLGDRIDVHAGVKIRIDGHLINL
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEECCCEEEEECCEEEEECCHHHHH
LHAQKEVCRVLMYYKPEGELCTRHDPEGRPTVFDRLPRLTGSRWIAVGRLDINTSGLLLF
HHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEECCCCEEEE
TTDGELANRLMHPSREVEREYSVRVFGQVDEAMLHRLKKGVQLEDGPANFKEIKFAGGVG
ECCHHHHHHHCCCCHHHHHHHCEEEECCHHHHHHHHHHCCCEECCCCCCHHEEEEECCCC
MNQWFDVTLMEGRNREVRRLWESQGVQVSRLIRTRYGNISLMKSLPRGGWEEMDLTNVNY
CCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHH
LRELVGLPAEVETKLDVTKPRRRAKTGQIRKAVKRYAELNKRYKK
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100