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The map label for this gene is trpB

Identifier: 15602443

GI number: 15602443

Start: 665932

End: 667140

Strand: Reverse

Name: trpB

Synonym: PM0578

Alternate gene names: 15602443

Gene position: 667140-665932 (Counterclockwise)

Preceding gene: 15602444

Following gene: 15602442

Centisome position: 29.55

GC content: 45.99

Gene sequence:

>1209_bases
ATGTCAGAAACCTTACTTAATCCCTATTTCGGCGAATTTGGAGGAATGTATGTTCCCGAAATTCTGATGCCGGTGTTGAA
AAATCTCGAAAAAGCCTTTGTTGAGGCACAACAAGATCCCACATTTAAAGAAACCTTCCTCGATTTATTGAAGAACTATG
CAGGTCGCCCAACTGCGCTGACTCGCTGTCGCAATCTCACTCAAGGCAGTAAAACCAAACTGTACCTGAAGCGTGAAGAT
TTATTGCACGGCGGTGCGCATAAAACCAATCAAGTATTAGGGCAAATCTTATTAGCGAAACGGATGGGAAAAACCCGTAT
TATTGCGGAAACAGGGGCGGGACAACATGGCGTAGCAACAGCTCTCGCTTGTGCCATGCTCGGTATGCCATGCCAAATCT
ACATGGGCGCAAAAGATGTTGAACGTCAATCGCCGAATGTATTTCGTATGCGTTTAATGGGGGCGAATGTGACTGCGGTA
ACGAAAGGCTCAGCCTCATTAAAAGACGCCTGCTGTGAAGCCATGCGTGATTGGGCAGAAAACTATGAACACACTCATTA
TTTACTCGGCACGGCAGCAGGTCCTCATCCCTTTCCTACTATCGTGCGTGAGTTCCAAAAAATCATTGGTGAAGAAACTA
AGCAACAAATTTTAGCACGCGAAGGGCGTTTACCTGATGCAGTCATCGCCGCGGTTGGTGGGGGTTCAAATGCCATTGGT
ATGTTCAATGACTTTATCGAGGAAACCAGCGTACGTTTAATTGGTGTTGAACCAGCGGGCAAAGGAATTGCCACTGGACA
ACATGGCGCTCCACTAGGTCATGGCACAACAGGAATCTATTTTGGTATGAAAGCGCCCTTGATGCAAACACCAGATGGAC
AAATTGAAGAATCGTATTCGATTTCAGCAGGACTTGATTTTCCTTCTGTTGGTCCACAGCACGCACATTTACAAGCGATT
GGGCGTGCCCAATATGAATCCATTACCGATGATGAAGCCTTATCCGCCTTCCAAGCCTTAGCACGCCATGAAGGCATTAT
TCCCGCATTAGAAAGTGCCCATGCCCTCGCTTATGCCTTAAAACTGATTCAGCGCCAGCCTGAAAAAGAACAACTCTTGG
TGGTCAATTTATCTGGACGAGGTGATAAAGATATTTTCACCGTAGATCGCATTTTATCGCAAAAAGGAGTTTCATATGCG
CCGTTTTGA

Upstream 100 bases:

>100_bases
TACACTCTGAACTAGTTTAATAGTGCAATATTCAAAAGTGAAATAGTTTCAATTTTATCCATCACAACGATTTATTATTA
TATTGTTTAAAGGAATAACC

Downstream 100 bases:

>100_bases
TACCCTCTTTTCACAATTACAACAAAAACAACAAGGTGCATTTGTCCCTTTTGTCACGCTATGCGATCCTAATCTGGCAC
AGTCATTTGAGATTATTCAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSETLLNPYFGEFGGMYVPEILMPVLKNLEKAFVEAQQDPTFKETFLDLLKNYAGRPTALTRCRNLTQGSKTKLYLKRED
LLHGGAHKTNQVLGQILLAKRMGKTRIIAETGAGQHGVATALACAMLGMPCQIYMGAKDVERQSPNVFRMRLMGANVTAV
TKGSASLKDACCEAMRDWAENYEHTHYLLGTAAGPHPFPTIVREFQKIIGEETKQQILAREGRLPDAVIAAVGGGSNAIG
MFNDFIEETSVRLIGVEPAGKGIATGQHGAPLGHGTTGIYFGMKAPLMQTPDGQIEESYSISAGLDFPSVGPQHAHLQAI
GRAQYESITDDEALSAFQALARHEGIIPALESAHALAYALKLIQRQPEKEQLLVVNLSGRGDKDIFTVDRILSQKGVSYA
PF

Sequences:

>Translated_402_residues
MSETLLNPYFGEFGGMYVPEILMPVLKNLEKAFVEAQQDPTFKETFLDLLKNYAGRPTALTRCRNLTQGSKTKLYLKRED
LLHGGAHKTNQVLGQILLAKRMGKTRIIAETGAGQHGVATALACAMLGMPCQIYMGAKDVERQSPNVFRMRLMGANVTAV
TKGSASLKDACCEAMRDWAENYEHTHYLLGTAAGPHPFPTIVREFQKIIGEETKQQILAREGRLPDAVIAAVGGGSNAIG
MFNDFIEETSVRLIGVEPAGKGIATGQHGAPLGHGTTGIYFGMKAPLMQTPDGQIEESYSISAGLDFPSVGPQHAHLQAI
GRAQYESITDDEALSAFQALARHEGIIPALESAHALAYALKLIQRQPEKEQLLVVNLSGRGDKDIFTVDRILSQKGVSYA
PF
>Mature_401_residues
SETLLNPYFGEFGGMYVPEILMPVLKNLEKAFVEAQQDPTFKETFLDLLKNYAGRPTALTRCRNLTQGSKTKLYLKREDL
LHGGAHKTNQVLGQILLAKRMGKTRIIAETGAGQHGVATALACAMLGMPCQIYMGAKDVERQSPNVFRMRLMGANVTAVT
KGSASLKDACCEAMRDWAENYEHTHYLLGTAAGPHPFPTIVREFQKIIGEETKQQILAREGRLPDAVIAAVGGGSNAIGM
FNDFIEETSVRLIGVEPAGKGIATGQHGAPLGHGTTGIYFGMKAPLMQTPDGQIEESYSISAGLDFPSVGPQHAHLQAIG
RAQYESITDDEALSAFQALARHEGIIPALESAHALAYALKLIQRQPEKEQLLVVNLSGRGDKDIFTVDRILSQKGVSYAP
F

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=393, Percent_Identity=78.6259541984733, Blast_Score=646, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321412, Length=389, Percent_Identity=52.6992287917738, Blast_Score=417, Evalue=1e-117,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_PASMU (P54203)

Other databases:

- EMBL:   U22344
- EMBL:   AE004439
- RefSeq:   NP_245515.1
- ProteinModelPortal:   P54203
- SMR:   P54203
- GeneID:   1243925
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0578
- NMPDR:   fig|272843.1.peg.578
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   PMUL272843:PM0578-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43625; Mature: 43493

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETLLNPYFGEFGGMYVPEILMPVLKNLEKAFVEAQQDPTFKETFLDLLKNYAGRPTAL
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
TRCRNLTQGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRMGKTRIIAETGAGQHGVAT
HHHHHCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
ALACAMLGMPCQIYMGAKDVERQSPNVFRMRLMGANVTAVTKGSASLKDACCEAMRDWAE
HHHHHHHCCCCEEEECCHHHHHCCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHH
NYEHTHYLLGTAAGPHPFPTIVREFQKIIGEETKQQILAREGRLPDAVIAAVGGGSNAIG
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCHH
MFNDFIEETSVRLIGVEPAGKGIATGQHGAPLGHGTTGIYFGMKAPLMQTPDGQIEESYS
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCE
ISAGLDFPSVGPQHAHLQAIGRAQYESITDDEALSAFQALARHEGIIPALESAHALAYAL
EECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KLIQRQPEKEQLLVVNLSGRGDKDIFTVDRILSQKGVSYAPF
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SETLLNPYFGEFGGMYVPEILMPVLKNLEKAFVEAQQDPTFKETFLDLLKNYAGRPTAL
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHH
TRCRNLTQGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRMGKTRIIAETGAGQHGVAT
HHHHHCCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
ALACAMLGMPCQIYMGAKDVERQSPNVFRMRLMGANVTAVTKGSASLKDACCEAMRDWAE
HHHHHHHCCCCEEEECCHHHHHCCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHH
NYEHTHYLLGTAAGPHPFPTIVREFQKIIGEETKQQILAREGRLPDAVIAAVGGGSNAIG
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCHH
MFNDFIEETSVRLIGVEPAGKGIATGQHGAPLGHGTTGIYFGMKAPLMQTPDGQIEESYS
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCE
ISAGLDFPSVGPQHAHLQAIGRAQYESITDDEALSAFQALARHEGIIPALESAHALAYAL
EECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KLIQRQPEKEQLLVVNLSGRGDKDIFTVDRILSQKGVSYAPF
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8581158; 11248100