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The map label for this gene is trpA

Identifier: 15602442

GI number: 15602442

Start: 665139

End: 665945

Strand: Reverse

Name: trpA

Synonym: PM0577

Alternate gene names: 15602442

Gene position: 665945-665139 (Counterclockwise)

Preceding gene: 15602443

Following gene: 15602440

Centisome position: 29.5

GC content: 42.5

Gene sequence:

>807_bases
ATGCGCCGTTTTGATACCCTCTTTTCACAATTACAACAAAAACAACAAGGTGCATTTGTCCCTTTTGTCACGCTATGCGA
TCCTAATCTGGCACAGTCATTTGAGATTATTCAAACCTTAGTGGAAAATGGTGCAGACGCCTTAGAATTAGGCTTTCCTT
TTTCTGATCCGATGTTAGATGGTCCAGTGATTCAAGCCGCTAACCAACGTGCGTTAGTCGCAGGTTGTAGCACAGATGCT
AGTTTTCAGTTATTAACACAAGTACGATCAACTTATCCGACACTACCAATAAGTTTACTGCTTTGTGCCAATCTGGTTTA
TGCACAAGGCATCGAACGTTTTTATCAACGTTGTGCTGAAGTGGGCGTGGATGCGGTATTAATTGCGGATTTACCATTAT
TCGCCCATCGAGAGTTTACGGTTATGGCTGAAAAATATAGCATTCAGCAGGTTTTTATCTGTCCGCCTAATGCCTGTGAA
GACACGATCAAACGTGTCGCTCAACAAACACAAGGTTACACCTATTTAGTTTCTCGTTCAGGTGTAACAGGAGTAGAAAA
TCAACAATCTGCTGACAACTTAAAAACACTCGTCGAGCGCCTCAAAGCCTATCAAGCTCCCCCTATTTTGCAAGGCTTTG
GTATTTCGCAACCACAGCAAGTTAGCGATGTGTTAAAAATAGGCGTCAATGGTGCCATTTCGGGTTCCGCTACCGTTCAA
ATTATTGAAAAATATCACCATGACCCACAAGCCTGCTTAGCTGAACTAGCGCAGTTTACGCAAAAAATGAAACAAGCGAC
CACATAA

Upstream 100 bases:

>100_bases
CAGCCTGAAAAAGAACAACTCTTGGTGGTCAATTTATCTGGACGAGGTGATAAAGATATTTTCACCGTAGATCGCATTTT
ATCGCAAAAAGGAGTTTCAT

Downstream 100 bases:

>100_bases
AAAAATATAGCCCGATTTGGATTCAATCGGGCTATATTTTAAAATTGCTGAGTCAGGCTAAATTTGACATTCCTACCTGT
TCCTGAAGCTAATTCTCCTA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRRFDTLFSQLQQKQQGAFVPFVTLCDPNLAQSFEIIQTLVENGADALELGFPFSDPMLDGPVIQAANQRALVAGCSTDA
SFQLLTQVRSTYPTLPISLLLCANLVYAQGIERFYQRCAEVGVDAVLIADLPLFAHREFTVMAEKYSIQQVFICPPNACE
DTIKRVAQQTQGYTYLVSRSGVTGVENQQSADNLKTLVERLKAYQAPPILQGFGISQPQQVSDVLKIGVNGAISGSATVQ
IIEKYHHDPQACLAELAQFTQKMKQATT

Sequences:

>Translated_268_residues
MRRFDTLFSQLQQKQQGAFVPFVTLCDPNLAQSFEIIQTLVENGADALELGFPFSDPMLDGPVIQAANQRALVAGCSTDA
SFQLLTQVRSTYPTLPISLLLCANLVYAQGIERFYQRCAEVGVDAVLIADLPLFAHREFTVMAEKYSIQQVFICPPNACE
DTIKRVAQQTQGYTYLVSRSGVTGVENQQSADNLKTLVERLKAYQAPPILQGFGISQPQQVSDVLKIGVNGAISGSATVQ
IIEKYHHDPQACLAELAQFTQKMKQATT
>Mature_268_residues
MRRFDTLFSQLQQKQQGAFVPFVTLCDPNLAQSFEIIQTLVENGADALELGFPFSDPMLDGPVIQAANQRALVAGCSTDA
SFQLLTQVRSTYPTLPISLLLCANLVYAQGIERFYQRCAEVGVDAVLIADLPLFAHREFTVMAEKYSIQQVFICPPNACE
DTIKRVAQQTQGYTYLVSRSGVTGVENQQSADNLKTLVERLKAYQAPPILQGFGISQPQQVSDVLKIGVNGAISGSATVQ
IIEKYHHDPQACLAELAQFTQKMKQATT

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=267, Percent_Identity=52.059925093633, Blast_Score=277, Evalue=6e-76,
Organism=Saccharomyces cerevisiae, GI6321412, Length=235, Percent_Identity=28.936170212766, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_PASMU (P57854)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245514.1
- ProteinModelPortal:   P57854
- SMR:   P57854
- GeneID:   1243924
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0577
- NMPDR:   fig|272843.1.peg.577
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   PMUL272843:PM0577-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29439; Mature: 29439

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFDTLFSQLQQKQQGAFVPFVTLCDPNLAQSFEIIQTLVENGADALELGFPFSDPMLD
CCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQAANQRALVAGCSTDASFQLLTQVRSTYPTLPISLLLCANLVYAQGIERFYQRCAE
CHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGVDAVLIADLPLFAHREFTVMAEKYSIQQVFICPPNACEDTIKRVAQQTQGYTYLVSRS
CCCCEEEEECCCHHHCCHHEEEEHHHCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECC
GVTGVENQQSADNLKTLVERLKAYQAPPILQGFGISQPQQVSDVLKIGVNGAISGSATVQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
IIEKYHHDPQACLAELAQFTQKMKQATT
HHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRFDTLFSQLQQKQQGAFVPFVTLCDPNLAQSFEIIQTLVENGADALELGFPFSDPMLD
CCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQAANQRALVAGCSTDASFQLLTQVRSTYPTLPISLLLCANLVYAQGIERFYQRCAE
CHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VGVDAVLIADLPLFAHREFTVMAEKYSIQQVFICPPNACEDTIKRVAQQTQGYTYLVSRS
CCCCEEEEECCCHHHCCHHEEEEHHHCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECC
GVTGVENQQSADNLKTLVERLKAYQAPPILQGFGISQPQQVSDVLKIGVNGAISGSATVQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
IIEKYHHDPQACLAELAQFTQKMKQATT
HHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100