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The map label for this gene is hemK

Identifier: 15602421

GI number: 15602421

Start: 639843

End: 640739

Strand: Direct

Name: hemK

Synonym: PM0556

Alternate gene names: 15602421

Gene position: 639843-640739 (Clockwise)

Preceding gene: 15602420

Following gene: 15602422

Centisome position: 28.34

GC content: 41.58

Gene sequence:

>897_bases
ATGACGTATCAAGAATGGCGACAATTCGCTGAACATGTATTGATGAAAAATAAGGAGAACGATCCTTTTTTAGATGTAAA
AAGTGAGAGTGTTCTTTTATTACAAACCGTCACTAAACGTTCAAAAGCGAGTATTCTCGCTTTTTCAGAAACAGTGCTAA
CGGAAGTAGAATTACAGCAGTTAGCACAACTTTTAATGCGTCGTGCTAAAGGTGAGCCGATTGCCTACATTCTCGGGGAA
AAAGCATTTTGGTCATTGTCATTGAAAGTGTCAGAACACACATTGATTCCTCGTCCAGATACCGAAGTTTTAGTGGAGCA
TGCGTTAGACTTCGCAAAACAACGAGTGACTTCAGCGCACGTTTCCGGCGAACTTTCAATACTCGATTTAGGCACAGGAA
CGGGGGCAATTGCATTAGCCTTAGCGGCAGAATTGACGCCGTTAACGCAAAAGTGCGGTATCAATTTAAATATTTTGGGT
GTTGATCGTATTGCAGAAGCGGTCGCCTTAGCAAAAGACAATGCCAAGCAAAATGAGTTAAAGGTTAACTTTTTACAAAG
TGTGTGGTTTGATGCACTTAATCCAGAGATTCGCTTTGATTTGATTGTCAGTAACCCCCCTTACATTGATAAAAACGACC
CGCATTTGACACAAGGTGATGTCCGTTTTGAGCCTTTATCTGCCTTAGTCGCAGCGGAAGAGGGGTATGCGGATATCCGC
CATATTATTGAACAAGCCCCTTTGTTTTTAAAGCCACAAGGAGCGTTATTACTCGAACATGGCTGGCAACAAGCTGAAAA
AGTGCGGTCAATTTTTCAAAAAAATTTATGGCATAACGTTGCGACGCTAAAAGATTATAGTGGGAATGAAAGGGTAACCT
TAGGTTGTTGGAGGTGA

Upstream 100 bases:

>100_bases
TATCAAGCAGATCAACTTGCGGCCTTGTCAGAGCAAGCCTAATAGATATTAAAGCGAAATCTGTAAGTAATGGATATAAA
CATATTCAAATGATTAAATA

Downstream 100 bases:

>100_bases
GTAGAATGAACTATGTGAAAAAAGCCCTATATGATGAAATGTTGAGTTTTTACCATACAACACTCGGTATGGAACATGAA
GATCGGTTACGTCTTCGTGG

Product: hypothetical protein

Products: NA

Alternate protein names: M.PmuHemKP

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTYQEWRQFAEHVLMKNKENDPFLDVKSESVLLLQTVTKRSKASILAFSETVLTEVELQQLAQLLMRRAKGEPIAYILGE
KAFWSLSLKVSEHTLIPRPDTEVLVEHALDFAKQRVTSAHVSGELSILDLGTGTGAIALALAAELTPLTQKCGINLNILG
VDRIAEAVALAKDNAKQNELKVNFLQSVWFDALNPEIRFDLIVSNPPYIDKNDPHLTQGDVRFEPLSALVAAEEGYADIR
HIIEQAPLFLKPQGALLLEHGWQQAEKVRSIFQKNLWHNVATLKDYSGNERVTLGCWR

Sequences:

>Translated_298_residues
MTYQEWRQFAEHVLMKNKENDPFLDVKSESVLLLQTVTKRSKASILAFSETVLTEVELQQLAQLLMRRAKGEPIAYILGE
KAFWSLSLKVSEHTLIPRPDTEVLVEHALDFAKQRVTSAHVSGELSILDLGTGTGAIALALAAELTPLTQKCGINLNILG
VDRIAEAVALAKDNAKQNELKVNFLQSVWFDALNPEIRFDLIVSNPPYIDKNDPHLTQGDVRFEPLSALVAAEEGYADIR
HIIEQAPLFLKPQGALLLEHGWQQAEKVRSIFQKNLWHNVATLKDYSGNERVTLGCWR
>Mature_297_residues
TYQEWRQFAEHVLMKNKENDPFLDVKSESVLLLQTVTKRSKASILAFSETVLTEVELQQLAQLLMRRAKGEPIAYILGEK
AFWSLSLKVSEHTLIPRPDTEVLVEHALDFAKQRVTSAHVSGELSILDLGTGTGAIALALAAELTPLTQKCGINLNILGV
DRIAEAVALAKDNAKQNELKVNFLQSVWFDALNPEIRFDLIVSNPPYIDKNDPHLTQGDVRFEPLSALVAAEEGYADIRH
IIEQAPLFLKPQGALLLEHGWQQAEKVRSIFQKNLWHNVATLKDYSGNERVTLGCWR

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family

Homologues:

Organism=Homo sapiens, GI7705409, Length=253, Percent_Identity=34.7826086956522, Blast_Score=108, Evalue=5e-24,
Organism=Escherichia coli, GI1787463, Length=296, Percent_Identity=51.6891891891892, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI87082085, Length=214, Percent_Identity=28.9719626168224, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324266, Length=185, Percent_Identity=31.8918918918919, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582226, Length=273, Percent_Identity=27.1062271062271, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMK_PASMU (Q9CN82)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245493.1
- ProteinModelPortal:   Q9CN82
- SMR:   Q9CN82
- GeneID:   1243903
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0556
- NMPDR:   fig|272843.1.peg.556
- HOGENOM:   HBG732041
- OMA:   ISAVFTM
- ProtClustDB:   PRK09328
- BioCyc:   PMUL272843:PM0556-MONOMER
- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- TIGRFAMs:   TIGR00536
- TIGRFAMs:   TIGR03534

Pfam domain/function: PF05175 MTS

EC number: 2.1.1.-

Molecular weight: Translated: 33429; Mature: 33298

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 162-162 BINDING 189-189 BINDING 205-205

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYQEWRQFAEHVLMKNKENDPFLDVKSESVLLLQTVTKRSKASILAFSETVLTEVELQQ
CCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEHHHHHHHHHHEEEEHHHHHHHHHHHH
LAQLLMRRAKGEPIAYILGEKAFWSLSLKVSEHTLIPRPDTEVLVEHALDFAKQRVTSAH
HHHHHHHHHCCCCEEEEECCCEEEEEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHH
VSGELSILDLGTGTGAIALALAAELTPLTQKCGINLNILGVDRIAEAVALAKDNAKQNEL
CCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCHHH
KVNFLQSVWFDALNPEIRFDLIVSNPPYIDKNDPHLTQGDVRFEPLSALVAAEEGYADIR
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCHHHHH
HIIEQAPLFLKPQGALLLEHGWQQAEKVRSIFQKNLWHNVATLKDYSGNERVTLGCWR
HHHHHCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
>Mature Secondary Structure 
TYQEWRQFAEHVLMKNKENDPFLDVKSESVLLLQTVTKRSKASILAFSETVLTEVELQQ
CHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEHHHHHHHHHHEEEEHHHHHHHHHHHH
LAQLLMRRAKGEPIAYILGEKAFWSLSLKVSEHTLIPRPDTEVLVEHALDFAKQRVTSAH
HHHHHHHHHCCCCEEEEECCCEEEEEEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHH
VSGELSILDLGTGTGAIALALAAELTPLTQKCGINLNILGVDRIAEAVALAKDNAKQNEL
CCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCHHH
KVNFLQSVWFDALNPEIRFDLIVSNPPYIDKNDPHLTQGDVRFEPLSALVAAEEGYADIR
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCHHHHH
HIIEQAPLFLKPQGALLLEHGWQQAEKVRSIFQKNLWHNVATLKDYSGNERVTLGCWR
HHHHHCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100