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The map label for this gene is prfA [H]

Identifier: 15602420

GI number: 15602420

Start: 638702

End: 639784

Strand: Direct

Name: prfA [H]

Synonym: PM0555

Alternate gene names: 15602420

Gene position: 638702-639784 (Clockwise)

Preceding gene: 15602419

Following gene: 15602421

Centisome position: 28.29

GC content: 41.27

Gene sequence:

>1083_bases
ATGAAACCCACAATTATCAGTAAATTAGAAAGTTTGAAAGAGCGCCACGAGGAGCTAGAGGCACTATTGGGTGAGGCTTC
TGTTATCAGTGATCAAGATAAATTTCGAGCTTACTCAAAAGAATATGCACAATTAGAAGATGTGGTGAAATGTTTTGCTC
GTTGGAATCAATTAAACAGTAATATGGAAGAAGCGCAACTTTTACTCGATGATCCTAGCATGAAAGAAATGGCGCAAGAA
GAAATTGAAGCATGTAAAACAGAAATCGAACAAGTTGAACAACAATTACAAATTTTATTGCTGCCAAAAGATCCAAATGA
TGAATATAACTGCTATCTAGAAATTCGTGCAGGAACAGGTGGAGATGAAGCCGGGATTTTTGCTGGCGATTTATTCCGTA
TGTATAGCCGTTATGCAGAAAGTAAACGTTGGAAAGTAGAAGTATTAAGTGCCAATGAAAGTGAGCAGGGTGGTTATAAA
GAAATTATTGTGAAGGTCAATGGTGAAGGCGTTTATGGGCAATTAAAATTTGAGTCAGGTGGTCACCGTGTTCAGCGTGT
TCCTAAGACCGAATCACAAGGCCGTATTCATACCTCTGCTTGTACCGTTGCCGTGATGCCAGAGTTACCTGAGTCTGAGT
TACCAGAAATCAATCCATCTGATTTACGTATTGATACTTACCGTTCTTCAGGGGCGGGTGGTCAGCACGTGAATACCACA
GATTCGGCGGTGCGTATTACTCATATTCCAACGGGAATTGTGGTCGAGTGTCAAGATGAGCGTTCGCAGCATAAAAACAA
AGCGAAAGCCATGTCTGTATTAGCTTCACGTATTGTTCAGGCGGAAAAAGAACGTCAAGAGCAAGCCCAAGCGGATACCC
GTCGTAATTTATTGGGTTCTGGTGACCGATCAGACAAAATCCGCACCTACAACTATCCACAAGGTCGTGTGACAGATCAC
CGTATTAACTTAACGGTTTACCGTTTAGATGAAGTGATGAATGGTAAAATTGATGAATTAATTCAACCGATTATTACCGA
ATATCAAGCAGATCAACTTGCGGCCTTGTCAGAGCAAGCCTAA

Upstream 100 bases:

>100_bases
GAAACAGATTATTGGCAATATTTCTATAATTTCTTTCACAATACATGTTAGAATTATTCGATTTTTTATTTCGTTTTTGT
TAATTCAGTAAGGTTAAGGA

Downstream 100 bases:

>100_bases
TAGATATTAAAGCGAAATCTGTAAGTAATGGATATAAACATATTCAAATGATTAAATAATGACGTATCAAGAATGGCGAC
AATTCGCTGAACATGTATTG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKPTIISKLESLKERHEELEALLGEASVISDQDKFRAYSKEYAQLEDVVKCFARWNQLNSNMEEAQLLLDDPSMKEMAQE
EIEACKTEIEQVEQQLQILLLPKDPNDEYNCYLEIRAGTGGDEAGIFAGDLFRMYSRYAESKRWKVEVLSANESEQGGYK
EIIVKVNGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESELPEINPSDLRIDTYRSSGAGGQHVNTT
DSAVRITHIPTGIVVECQDERSQHKNKAKAMSVLASRIVQAEKERQEQAQADTRRNLLGSGDRSDKIRTYNYPQGRVTDH
RINLTVYRLDEVMNGKIDELIQPIITEYQADQLAALSEQA

Sequences:

>Translated_360_residues
MKPTIISKLESLKERHEELEALLGEASVISDQDKFRAYSKEYAQLEDVVKCFARWNQLNSNMEEAQLLLDDPSMKEMAQE
EIEACKTEIEQVEQQLQILLLPKDPNDEYNCYLEIRAGTGGDEAGIFAGDLFRMYSRYAESKRWKVEVLSANESEQGGYK
EIIVKVNGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESELPEINPSDLRIDTYRSSGAGGQHVNTT
DSAVRITHIPTGIVVECQDERSQHKNKAKAMSVLASRIVQAEKERQEQAQADTRRNLLGSGDRSDKIRTYNYPQGRVTDH
RINLTVYRLDEVMNGKIDELIQPIITEYQADQLAALSEQA
>Mature_360_residues
MKPTIISKLESLKERHEELEALLGEASVISDQDKFRAYSKEYAQLEDVVKCFARWNQLNSNMEEAQLLLDDPSMKEMAQE
EIEACKTEIEQVEQQLQILLLPKDPNDEYNCYLEIRAGTGGDEAGIFAGDLFRMYSRYAESKRWKVEVLSANESEQGGYK
EIIVKVNGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESELPEINPSDLRIDTYRSSGAGGQHVNTT
DSAVRITHIPTGIVVECQDERSQHKNKAKAMSVLASRIVQAEKERQEQAQADTRRNLLGSGDRSDKIRTYNYPQGRVTDH
RINLTVYRLDEVMNGKIDELIQPIITEYQADQLAALSEQA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=305, Percent_Identity=48.1967213114754, Blast_Score=285, Evalue=7e-77,
Organism=Homo sapiens, GI34577120, Length=361, Percent_Identity=36.8421052631579, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI166795305, Length=210, Percent_Identity=48.5714285714286, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=74.6518105849582, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=39.2857142857143, Blast_Score=224, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17542784, Length=322, Percent_Identity=34.1614906832298, Blast_Score=195, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=315, Percent_Identity=42.5396825396825, Blast_Score=255, Evalue=9e-69,
Organism=Drosophila melanogaster, GI19921226, Length=327, Percent_Identity=40.6727828746177, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40653; Mature: 40653

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTIISKLESLKERHEELEALLGEASVISDQDKFRAYSKEYAQLEDVVKCFARWNQLNS
CCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NMEEAQLLLDDPSMKEMAQEEIEACKTEIEQVEQQLQILLLPKDPNDEYNCYLEIRAGTG
CHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEEEEEECCC
GDEAGIFAGDLFRMYSRYAESKRWKVEVLSANESEQGGYKEIIVKVNGEGVYGQLKFESG
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEEECCC
GHRVQRVPKTESQGRIHTSACTVAVMPELPESELPEINPSDLRIDTYRSSGAGGQHVNTT
CCHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
DSAVRITHIPTGIVVECQDERSQHKNKAKAMSVLASRIVQAEKERQEQAQADTRRNLLGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMNGKIDELIQPIITEYQADQLAALSEQA
CCCCCCEEEEECCCCCCCCEEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPTIISKLESLKERHEELEALLGEASVISDQDKFRAYSKEYAQLEDVVKCFARWNQLNS
CCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NMEEAQLLLDDPSMKEMAQEEIEACKTEIEQVEQQLQILLLPKDPNDEYNCYLEIRAGTG
CHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEEEEEECCC
GDEAGIFAGDLFRMYSRYAESKRWKVEVLSANESEQGGYKEIIVKVNGEGVYGQLKFESG
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEEECCC
GHRVQRVPKTESQGRIHTSACTVAVMPELPESELPEINPSDLRIDTYRSSGAGGQHVNTT
CCHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
DSAVRITHIPTGIVVECQDERSQHKNKAKAMSVLASRIVQAEKERQEQAQADTRRNLLGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMNGKIDELIQPIITEYQADQLAALSEQA
CCCCCCEEEEECCCCCCCCEEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA