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The map label for this gene is glgx [H]

Identifier: 15602407

GI number: 15602407

Start: 620710

End: 622698

Strand: Direct

Name: glgx [H]

Synonym: PM0542

Alternate gene names: 15602407

Gene position: 620710-622698 (Clockwise)

Preceding gene: 15602406

Following gene: 15602408

Centisome position: 27.5

GC content: 42.38

Gene sequence:

>1989_bases
ATGAATACGATAAAAAGTGGGAAACCCTCTCCGTTGGGGTGTTCAAAACAAACGGAAGAAGGGCAAGACGGCTATAACTT
CGCGCTTTTTTCTCAAGCTGCGCGACGAGTTGAGTTGTGTTTGTTCAGCGAAGGGCAAGAGCGACGGGTTGATTTACAAC
CAAGTGATGATGGGATTTGGCATATTTGGTTGAGCGATGTGTCTGTTGGGACAGAATATGCTTATCGAATTTCGGGTAAA
ACTGACCGCACTTTAGCAAACTCGAATAAGTTGATGCTTGATCCCTATGCGAAAGCGGTTGTGGGCAAACCGGATCTGAG
TACACCGGAAAAACGTGCTTGGTTTGATCTTAATGACCCTCGAGATAACGCACATCTTGCGCCGAAGGCAGTTATTATAG
ATGAGGCTTTTGATTGGCAAGGAGATAAGCCACTACGAACCCCTTGGGCACAAACAGTGATTTATGAAATGCATGTCAAA
GGCTTTAGCCAACTACGTGAAGATTTACCTGAAGCCTTGCGAGGGACTTATGCGGGTTTAGCACATCCTAATATGATTGC
GTATTTGCAAGACTTAGGCGTGACAGCAGTCGAGCTATTACCAGTTCAATATCAAGCGGATGAAGTGCATTTACAAGAAA
AAGGTTTAGCCAATTATTGGGGGTATAACCCATTAGCGATGTTTGCGCTAGAACCTCGTTATAGCTCACAGCACAGCAGT
ATGGGAGCATTGCATGAGTTTAAAACGATGGTTAAAGCATTACATCAAGCGGGTATCGAAGTAATTTTGGATGTCGTGTT
TAACCATACGGCTGAGTCAGAAAAGTATTTCCCGACGTTCAGTCAGCGTGGCATTGATGATGAAACTTACTATTGGCAAG
ACGCACAAGGATATTATCACAATTGGACGGGCTGCGGTAATACACTTAATTTGTCACAGCCGATCACGCGTCGTTGGGTG
CTAGATTGTTTATGCTATTGGGTTGAGGCTTGCCATGTAGACGGTTTTCGTTTTGATTTAGGTTCAGTACTTGGGCGTGA
GGGAGAAGGGGGCTTTAACCCACAAGCACAATTATTTGCCGATATTCAAGCAGAACCTCGCTTACAAGGCTGCAAATTGA
TTGCTGAACCTTGGGATATTGGTGATTTTGGCTATCAAATGGGTAACTTCCCCGCTTATTTTGCTGAATGGAATGATCGT
TTTCGTGATGATGTGACGCGTTTTTGGTTGTGGAAGAGTGGTGAAAGTGGTGCATTTGCACAACGTTTAGCGGGGTCAAG
TGATTTATTCCAACAAACCCGCCAAGTACACAATTCGATTAATTTCATTACCGCACATGACGGTTTTACTTTACGTGATT
TAGTCAGTTATAACGAGAAACACAATTGGGCAAATGGTGAAAATAACCGAGATGGGCGTAATCACAATTACAGTTATAAC
CACGGTGTTGAAGGGATCGACAATGTGCCAAGTGCGGTAGAAAATGGGCGTATTTTAAGTAGCATTGCCTTATTGATGAA
TTTACTCTTGGCAAATGGCACGCCGATGCTCCTTGCGGGGGACGAATTTGGCAATTCTCAGTTTGGTAATAATAATGCCT
ATTGCCAAGATAATGAAATTGCTTGGCTTAAATGGCAAGATTTTAATCCTGTATTATTTAATGCGACTAAGCAAACGATT
GCATTGCGAAAACAAATAGCGAGTTTACAACAAGACTGTTGGTGGTCTGAGGTAAATGTGCAATGGCTCAATACAAAAGG
GGAGCCAATGACAATCGCGGATTGGCATAATCAAGAAAGCAAAGCCTTACAAGTGCTTTTAGATAACAAATGGCTTTTTT
TGATTAATGCGAAACAAAAATTACAAACTTTTTCGTTACCAGAAGGTGATTGGGAAGTTATGTATTGTGCAACAAACTTA
ACCACTCAAGGCGGTTTTATCGAGTTGGATGATGTCACTTTTGGTGTGTTGCAACAGTTAAATAAGTAG

Upstream 100 bases:

>100_bases
ATTTATTTGAAATATCAAGGGGAATAACGGTGCGTGATAGGCTGTCCTATTCCATATATAAGCAGTCAAAATGTGTAGTC
AAAGTGATAATGTTGTACAA

Downstream 100 bases:

>100_bases
TAATTATATGTAAACAATAATAAGGGTTGTTGATATGAGGTTGTGCAACGTTACCCAATAATGGAGGTCATTATGAATAG
TAGCATTGTAAAATTGCCTA

Product: Glgx

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MNTIKSGKPSPLGCSKQTEEGQDGYNFALFSQAARRVELCLFSEGQERRVDLQPSDDGIWHIWLSDVSVGTEYAYRISGK
TDRTLANSNKLMLDPYAKAVVGKPDLSTPEKRAWFDLNDPRDNAHLAPKAVIIDEAFDWQGDKPLRTPWAQTVIYEMHVK
GFSQLREDLPEALRGTYAGLAHPNMIAYLQDLGVTAVELLPVQYQADEVHLQEKGLANYWGYNPLAMFALEPRYSSQHSS
MGALHEFKTMVKALHQAGIEVILDVVFNHTAESEKYFPTFSQRGIDDETYYWQDAQGYYHNWTGCGNTLNLSQPITRRWV
LDCLCYWVEACHVDGFRFDLGSVLGREGEGGFNPQAQLFADIQAEPRLQGCKLIAEPWDIGDFGYQMGNFPAYFAEWNDR
FRDDVTRFWLWKSGESGAFAQRLAGSSDLFQQTRQVHNSINFITAHDGFTLRDLVSYNEKHNWANGENNRDGRNHNYSYN
HGVEGIDNVPSAVENGRILSSIALLMNLLLANGTPMLLAGDEFGNSQFGNNNAYCQDNEIAWLKWQDFNPVLFNATKQTI
ALRKQIASLQQDCWWSEVNVQWLNTKGEPMTIADWHNQESKALQVLLDNKWLFLINAKQKLQTFSLPEGDWEVMYCATNL
TTQGGFIELDDVTFGVLQQLNK

Sequences:

>Translated_662_residues
MNTIKSGKPSPLGCSKQTEEGQDGYNFALFSQAARRVELCLFSEGQERRVDLQPSDDGIWHIWLSDVSVGTEYAYRISGK
TDRTLANSNKLMLDPYAKAVVGKPDLSTPEKRAWFDLNDPRDNAHLAPKAVIIDEAFDWQGDKPLRTPWAQTVIYEMHVK
GFSQLREDLPEALRGTYAGLAHPNMIAYLQDLGVTAVELLPVQYQADEVHLQEKGLANYWGYNPLAMFALEPRYSSQHSS
MGALHEFKTMVKALHQAGIEVILDVVFNHTAESEKYFPTFSQRGIDDETYYWQDAQGYYHNWTGCGNTLNLSQPITRRWV
LDCLCYWVEACHVDGFRFDLGSVLGREGEGGFNPQAQLFADIQAEPRLQGCKLIAEPWDIGDFGYQMGNFPAYFAEWNDR
FRDDVTRFWLWKSGESGAFAQRLAGSSDLFQQTRQVHNSINFITAHDGFTLRDLVSYNEKHNWANGENNRDGRNHNYSYN
HGVEGIDNVPSAVENGRILSSIALLMNLLLANGTPMLLAGDEFGNSQFGNNNAYCQDNEIAWLKWQDFNPVLFNATKQTI
ALRKQIASLQQDCWWSEVNVQWLNTKGEPMTIADWHNQESKALQVLLDNKWLFLINAKQKLQTFSLPEGDWEVMYCATNL
TTQGGFIELDDVTFGVLQQLNK
>Mature_662_residues
MNTIKSGKPSPLGCSKQTEEGQDGYNFALFSQAARRVELCLFSEGQERRVDLQPSDDGIWHIWLSDVSVGTEYAYRISGK
TDRTLANSNKLMLDPYAKAVVGKPDLSTPEKRAWFDLNDPRDNAHLAPKAVIIDEAFDWQGDKPLRTPWAQTVIYEMHVK
GFSQLREDLPEALRGTYAGLAHPNMIAYLQDLGVTAVELLPVQYQADEVHLQEKGLANYWGYNPLAMFALEPRYSSQHSS
MGALHEFKTMVKALHQAGIEVILDVVFNHTAESEKYFPTFSQRGIDDETYYWQDAQGYYHNWTGCGNTLNLSQPITRRWV
LDCLCYWVEACHVDGFRFDLGSVLGREGEGGFNPQAQLFADIQAEPRLQGCKLIAEPWDIGDFGYQMGNFPAYFAEWNDR
FRDDVTRFWLWKSGESGAFAQRLAGSSDLFQQTRQVHNSINFITAHDGFTLRDLVSYNEKHNWANGENNRDGRNHNYSYN
HGVEGIDNVPSAVENGRILSSIALLMNLLLANGTPMLLAGDEFGNSQFGNNNAYCQDNEIAWLKWQDFNPVLFNATKQTI
ALRKQIASLQQDCWWSEVNVQWLNTKGEPMTIADWHNQESKALQVLLDNKWLFLINAKQKLQTFSLPEGDWEVMYCATNL
TTQGGFIELDDVTFGVLQQLNK

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=641, Percent_Identity=51.7940717628705, Blast_Score=616, Evalue=1e-177,
Organism=Escherichia coli, GI1789839, Length=352, Percent_Identity=25.5681818181818, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75237; Mature: 75237

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIKSGKPSPLGCSKQTEEGQDGYNFALFSQAARRVELCLFSEGQERRVDLQPSDDGIW
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCEEECCCCCCCEE
HIWLSDVSVGTEYAYRISGKTDRTLANSNKLMLDPYAKAVVGKPDLSTPEKRAWFDLNDP
EEEECCCCCCCEEEEEECCCCCCEECCCCCEEECHHHHHHCCCCCCCCCCCCCEECCCCC
RDNAHLAPKAVIIDEAFDWQGDKPLRTPWAQTVIYEMHVKGFSQLREDLPEALRGTYAGL
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AHPNMIAYLQDLGVTAVELLPVQYQADEVHLQEKGLANYWGYNPLAMFALEPRYSSQHSS
CCCHHHHHHHHCCCEEEEEEEEEECCCCEEHHHCCCHHHCCCCCEEEEEECCCCCCCCHH
MGALHEFKTMVKALHQAGIEVILDVVFNHTAESEKYFPTFSQRGIDDETYYWQDAQGYYH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCC
NWTGCGNTLNLSQPITRRWVLDCLCYWVEACHVDGFRFDLGSVLGREGEGGFNPQAQLFA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCCCCCCCCCHHEEE
DIQAEPRLQGCKLIAEPWDIGDFGYQMGNFPAYFAEWNDRFRDDVTRFWLWKSGESGAFA
ECCCCCCCCCCEEEECCCCCCHHHHHCCCCCEEHHHHHHHHHHHHHEEEEEECCCCCHHH
QRLAGSSDLFQQTRQVHNSINFITAHDGFTLRDLVSYNEKHNWANGENNRDGRNHNYSYN
HHHCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
HGVEGIDNVPSAVENGRILSSIALLMNLLLANGTPMLLAGDEFGNSQFGNNNAYCQDNEI
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCE
AWLKWQDFNPVLFNATKQTIALRKQIASLQQDCWWSEVNVQWLNTKGEPMTIADWHNQES
EEEEECCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCHH
KALQVLLDNKWLFLINAKQKLQTFSLPEGDWEVMYCATNLTTQGGFIELDDVTFGVLQQL
HEEEEEECCCEEEEEECHHHHHCCCCCCCCEEEEEEEECCCCCCCEEEECCHHHHHHHHH
NK
CC
>Mature Secondary Structure
MNTIKSGKPSPLGCSKQTEEGQDGYNFALFSQAARRVELCLFSEGQERRVDLQPSDDGIW
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCEEECCCCCCCEE
HIWLSDVSVGTEYAYRISGKTDRTLANSNKLMLDPYAKAVVGKPDLSTPEKRAWFDLNDP
EEEECCCCCCCEEEEEECCCCCCEECCCCCEEECHHHHHHCCCCCCCCCCCCCEECCCCC
RDNAHLAPKAVIIDEAFDWQGDKPLRTPWAQTVIYEMHVKGFSQLREDLPEALRGTYAGL
CCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AHPNMIAYLQDLGVTAVELLPVQYQADEVHLQEKGLANYWGYNPLAMFALEPRYSSQHSS
CCCHHHHHHHHCCCEEEEEEEEEECCCCEEHHHCCCHHHCCCCCEEEEEECCCCCCCCHH
MGALHEFKTMVKALHQAGIEVILDVVFNHTAESEKYFPTFSQRGIDDETYYWQDAQGYYH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCC
NWTGCGNTLNLSQPITRRWVLDCLCYWVEACHVDGFRFDLGSVLGREGEGGFNPQAQLFA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCCCCCCCCCHHEEE
DIQAEPRLQGCKLIAEPWDIGDFGYQMGNFPAYFAEWNDRFRDDVTRFWLWKSGESGAFA
ECCCCCCCCCCEEEECCCCCCHHHHHCCCCCEEHHHHHHHHHHHHHEEEEEECCCCCHHH
QRLAGSSDLFQQTRQVHNSINFITAHDGFTLRDLVSYNEKHNWANGENNRDGRNHNYSYN
HHHCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
HGVEGIDNVPSAVENGRILSSIALLMNLLLANGTPMLLAGDEFGNSQFGNNNAYCQDNEI
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCE
AWLKWQDFNPVLFNATKQTIALRKQIASLQQDCWWSEVNVQWLNTKGEPMTIADWHNQES
EEEEECCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCHH
KALQVLLDNKWLFLINAKQKLQTFSLPEGDWEVMYCATNLTTQGGFIELDDVTFGVLQQL
HEEEEEECCCEEEEEECHHHHHCCCCCCCCEEEEEEEECCCCCCCEEEECCHHHHHHHHH
NK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]