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The map label for this gene is phoB [H]

Identifier: 15602297

GI number: 15602297

Start: 507610

End: 508305

Strand: Reverse

Name: phoB [H]

Synonym: PM0432

Alternate gene names: 15602297

Gene position: 508305-507610 (Counterclockwise)

Preceding gene: 15602298

Following gene: 15602296

Centisome position: 22.52

GC content: 39.8

Gene sequence:

>696_bases
ATGACCAAAATACTGGTGGTTGAAGATGAAAGTGCGATTCGCGAAATGATCAGCCTATTCTTAACACAACAAGGTTATGA
CATTATTGAAGCGGCTGATTATCAAAGTGCGGTCAAAAAATTAGTAGAAAAGCCACAACTCATTTTATTAGACTGGATGT
TGCCCGGTCGTTCTGGTATCCAATTTATTCAGTTTTTGAAAAAACACGAAGACACCGAAAACATCCCTGTCATCATGCTG
ACTGCGCGTAGTGAAGAAGAAGATTGTATTACCGGCTTAAATACAGGCGCGGATGATTACATCACTAAACCTTTCTCACC
TAAAATCTTACTTGCCAGAATTGAAGCCATGCTCCGACGTGTTTATGAACAAAGCCAGAATAGCATTGAAATTGACGGGC
TACTTTTAGATCAAAATGCCTTGCGGGTGAGCTATCAAAAAAATGTGATTAACCTTAGCTCGACTGAATTTAAACTGCTT
AAATTTCTCATGACTCACCCTGAAAAAGTCTATACACGCGAACAGCTATTAGATTGTATTTGGGGAAATGATATTTATGT
GGAAGATAGAACGGTAGATAGCTATATTCGTCGCTTACGCAAAAGCCTTGAGCAATATGGTTTTGATCGCTATATCCAAA
CGGTACGTGGGGCGGGCTATCGTTTTTCGCCTCATTTTCAGGACGAGATGAAATGA

Upstream 100 bases:

>100_bases
GTATCATTTTGATTAAAATCAAACGAATTATGGACTTCTCCTCACGCTCTGATGAGGAGAAAGTTCGCTACTCCTATACG
GTGTATAAAGTGAGGTACAA

Downstream 100 bases:

>100_bases
AAAGAACCTTTTCAATTAAGTATTTTTTTATTGAATTAGCGATAAGCCTACTGATTGCCAGTCTTTTTTCCTATTTTACT
CAATCCTTTACATTCTGGTT

Product: PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTKILVVEDESAIREMISLFLTQQGYDIIEAADYQSAVKKLVEKPQLILLDWMLPGRSGIQFIQFLKKHEDTENIPVIML
TARSEEEDCITGLNTGADDYITKPFSPKILLARIEAMLRRVYEQSQNSIEIDGLLLDQNALRVSYQKNVINLSSTEFKLL
KFLMTHPEKVYTREQLLDCIWGNDIYVEDRTVDSYIRRLRKSLEQYGFDRYIQTVRGAGYRFSPHFQDEMK

Sequences:

>Translated_231_residues
MTKILVVEDESAIREMISLFLTQQGYDIIEAADYQSAVKKLVEKPQLILLDWMLPGRSGIQFIQFLKKHEDTENIPVIML
TARSEEEDCITGLNTGADDYITKPFSPKILLARIEAMLRRVYEQSQNSIEIDGLLLDQNALRVSYQKNVINLSSTEFKLL
KFLMTHPEKVYTREQLLDCIWGNDIYVEDRTVDSYIRRLRKSLEQYGFDRYIQTVRGAGYRFSPHFQDEMK
>Mature_230_residues
TKILVVEDESAIREMISLFLTQQGYDIIEAADYQSAVKKLVEKPQLILLDWMLPGRSGIQFIQFLKKHEDTENIPVIMLT
ARSEEEDCITGLNTGADDYITKPFSPKILLARIEAMLRRVYEQSQNSIEIDGLLLDQNALRVSYQKNVINLSSTEFKLLK
FLMTHPEKVYTREQLLDCIWGNDIYVEDRTVDSYIRRLRKSLEQYGFDRYIQTVRGAGYRFSPHFQDEMK

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=226, Percent_Identity=60.6194690265487, Blast_Score=279, Evalue=1e-76,
Organism=Escherichia coli, GI1789809, Length=231, Percent_Identity=37.2294372294372, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=37.7777777777778, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=37.117903930131, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=37.5565610859729, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=38.5650224215247, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1786911, Length=226, Percent_Identity=36.7256637168142, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790863, Length=236, Percent_Identity=33.8983050847458, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=33.1858407079646, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=29.4117647058824, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=29.7297297297297, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790552, Length=219, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI145693140, Length=239, Percent_Identity=31.7991631799163, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787229, Length=227, Percent_Identity=30.3964757709251, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788550, Length=106, Percent_Identity=33.0188679245283, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1788191, Length=124, Percent_Identity=29.0322580645161, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26972; Mature: 26841

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKILVVEDESAIREMISLFLTQQGYDIIEAADYQSAVKKLVEKPQLILLDWMLPGRSGI
CCEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHH
QFIQFLKKHEDTENIPVIMLTARSEEEDCITGLNTGADDYITKPFSPKILLARIEAMLRR
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VYEQSQNSIEIDGLLLDQNALRVSYQKNVINLSSTEFKLLKFLMTHPEKVYTREQLLDCI
HHHHCCCCEEEEEEEECCCHHEEEECCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHH
WGNDIYVEDRTVDSYIRRLRKSLEQYGFDRYIQTVRGAGYRFSPHFQDEMK
CCCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKILVVEDESAIREMISLFLTQQGYDIIEAADYQSAVKKLVEKPQLILLDWMLPGRSGI
CEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHH
QFIQFLKKHEDTENIPVIMLTARSEEEDCITGLNTGADDYITKPFSPKILLARIEAMLRR
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VYEQSQNSIEIDGLLLDQNALRVSYQKNVINLSSTEFKLLKFLMTHPEKVYTREQLLDCI
HHHHCCCCEEEEEEEECCCHHEEEECCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHH
WGNDIYVEDRTVDSYIRRLRKSLEQYGFDRYIQTVRGAGYRFSPHFQDEMK
CCCEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]