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The map label for this gene is dnaB

Identifier: 15602277

GI number: 15602277

Start: 482890

End: 484293

Strand: Direct

Name: dnaB

Synonym: PM0412

Alternate gene names: 15602277

Gene position: 482890-484293 (Clockwise)

Preceding gene: 15602276

Following gene: 15602278

Centisome position: 21.39

GC content: 41.38

Gene sequence:

>1404_bases
ATGGCAAAAAAGAACGCCCAAATTCCAAGTAAACCTCTTGAACAAGGCATTGTTCCGCCACATTCTTTAGAAGCAGAACA
GTCTGTTTTAGGCGGTATTTTGATTCACCCAGCTCATTGGGATAGCATTAGTGACATGCTCATTGCCGATGATTTCTATT
TAGCTGCCCATCGTGTCATTTTCCAAGAAATGGAAAATTTACTCCGTCAAGGGAAGCCAATTGATCTGATCAACCTCTAC
GAATTTTTACGCGAAAAGAATCTCAGTGAAGATGTTGGTGGCTTTGCGTATTTAGCTGAATTATCCAAAAACACACCAAG
TGTATCCAATATTGTTTCGTATGCCGCTATCGTGCGTGATCGTGCTATTTTGCGTGATTTGATTAGTGTCAGTAATGATG
TGGCGCAAAGCTGTTACACGACAAAAGGGATGGAGGTGAAAGATATTCTGGATGAGGCAGAACGAAAAGTCTTTGCCATT
TCGGAGAAACGCACCACTTCAAACGAAGGACCGCAAAATATCTGTGCGATTTTAGAAAAAACCATTGATCGTATTGAATT
GTTAAGTAAAACCGAAGGGCATAATGGTATCACAGGTGTCAGTACCGGTTTTGATGCGCTCGATAAGAAAACCGCCGGTT
TACAGCCTTCCGATCTCATCATTGTGGCAGCTCGTCCTTCAATGGGTAAAACGACTTTTGCAATGAATTTATGTGAAAAT
GCAGCACTCAAAAGTGATAAACCAGTTTTAGTGTTCAGTTTAGAGATGCCGGCAGAACAAATTATGATGCGTTCTCTTGC
TTCATTATCGCGTGTGGATCAAACCAAAATTCGTACTGGACAAGGGTTAGATCAAAGTGACTGGTCGAAGATTGGGAGTA
CAATGGGGCTGTTCGCTAATAAACCCAACTTATATATTGATGATTCATCCGGCTTAACCCCAACGGAATTACGTTCACGT
GCAAGACGAGTATATCGTGAAAACGGCGGCTTAAGTTTGATTATGGTGGACTATTTGCAATTAATGCGGGCACCGGGATT
TGCCGATAACCGTACCTTAGAGATTGCTGAAATTTCGCGTTCACTAAAAGCCTTAGCAAAAGAATTAGAAGTGCCAGTGA
TTGCCTTATCCCAGTTAAACCGTACCCTTGAAAATCGTGCTGATAAACGTCCAGTGAATTCTGATTTGCGTGAATCTGGT
TCCATTGAACAAGATGCGGACTTAATTATGTTTATTTATCGTGATGAAGTGTATAACGAAAACTCGGAAGATAAAGGCGT
GGCAGAAATTATTATTGGCAAACAGCGTAATGGTCCGATTGGACGCGTGAGATTGGCGTTTAGAGGACAATTCTCACGCT
TTGATAACTTAGCCGAACAACGAGATATAAGAGACGATTATTAA

Upstream 100 bases:

>100_bases
TTTTTATCCCTTCATTTTTGACAGAATTCGCGCTTAAATTAAAAGAAGAATTGGTATATCATATAGGCTAATTTTTAAAT
TCACGTATAGACCCATTCTT

Downstream 100 bases:

>100_bases
TGAATATGAAACCTGCCACAGCAAAAATCAGTTCTGTGGCATTAAAACACAACATTCAAACGATTAAACAAAAAGCCCCG
CAAAGTAAAATTATTGCTGT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MAKKNAQIPSKPLEQGIVPPHSLEAEQSVLGGILIHPAHWDSISDMLIADDFYLAAHRVIFQEMENLLRQGKPIDLINLY
EFLREKNLSEDVGGFAYLAELSKNTPSVSNIVSYAAIVRDRAILRDLISVSNDVAQSCYTTKGMEVKDILDEAERKVFAI
SEKRTTSNEGPQNICAILEKTIDRIELLSKTEGHNGITGVSTGFDALDKKTAGLQPSDLIIVAARPSMGKTTFAMNLCEN
AALKSDKPVLVFSLEMPAEQIMMRSLASLSRVDQTKIRTGQGLDQSDWSKIGSTMGLFANKPNLYIDDSSGLTPTELRSR
ARRVYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEVPVIALSQLNRTLENRADKRPVNSDLRESG
SIEQDADLIMFIYRDEVYNENSEDKGVAEIIIGKQRNGPIGRVRLAFRGQFSRFDNLAEQRDIRDDY

Sequences:

>Translated_467_residues
MAKKNAQIPSKPLEQGIVPPHSLEAEQSVLGGILIHPAHWDSISDMLIADDFYLAAHRVIFQEMENLLRQGKPIDLINLY
EFLREKNLSEDVGGFAYLAELSKNTPSVSNIVSYAAIVRDRAILRDLISVSNDVAQSCYTTKGMEVKDILDEAERKVFAI
SEKRTTSNEGPQNICAILEKTIDRIELLSKTEGHNGITGVSTGFDALDKKTAGLQPSDLIIVAARPSMGKTTFAMNLCEN
AALKSDKPVLVFSLEMPAEQIMMRSLASLSRVDQTKIRTGQGLDQSDWSKIGSTMGLFANKPNLYIDDSSGLTPTELRSR
ARRVYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEVPVIALSQLNRTLENRADKRPVNSDLRESG
SIEQDADLIMFIYRDEVYNENSEDKGVAEIIIGKQRNGPIGRVRLAFRGQFSRFDNLAEQRDIRDDY
>Mature_466_residues
AKKNAQIPSKPLEQGIVPPHSLEAEQSVLGGILIHPAHWDSISDMLIADDFYLAAHRVIFQEMENLLRQGKPIDLINLYE
FLREKNLSEDVGGFAYLAELSKNTPSVSNIVSYAAIVRDRAILRDLISVSNDVAQSCYTTKGMEVKDILDEAERKVFAIS
EKRTTSNEGPQNICAILEKTIDRIELLSKTEGHNGITGVSTGFDALDKKTAGLQPSDLIIVAARPSMGKTTFAMNLCENA
ALKSDKPVLVFSLEMPAEQIMMRSLASLSRVDQTKIRTGQGLDQSDWSKIGSTMGLFANKPNLYIDDSSGLTPTELRSRA
RRVYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEVPVIALSQLNRTLENRADKRPVNSDLRESGS
IEQDADLIMFIYRDEVYNENSEDKGVAEIIIGKQRNGPIGRVRLAFRGQFSRFDNLAEQRDIRDDY

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain

Homologues:

Organism=Escherichia coli, GI1790486, Length=441, Percent_Identity=69.3877551020408, Blast_Score=630, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAB_PASMU (Q9CNL6)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245349.1
- ProteinModelPortal:   Q9CNL6
- SMR:   Q9CNL6
- GeneID:   1243759
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0412
- NMPDR:   fig|272843.1.peg.412
- HOGENOM:   HBG734566
- OMA:   TGYTDLD
- BioCyc:   PMUL272843:PM0412-MONOMER
- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- Gene3D:   G3DSA:1.10.860.10
- SMART:   SM00382
- TIGRFAMs:   TIGR00665

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C; SSF48024 DNA_helicase_DnaB-like_N

EC number: =3.6.4.12

Molecular weight: Translated: 52012; Mature: 51881

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS51199 SF4_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKNAQIPSKPLEQGIVPPHSLEAEQSVLGGILIHPAHWDSISDMLIADDFYLAAHRVI
CCCCCCCCCCCHHHHCCCCCCCCCHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHH
FQEMENLLRQGKPIDLINLYEFLREKNLSEDVGGFAYLAELSKNTPSVSNIVSYAAIVRD
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RAILRDLISVSNDVAQSCYTTKGMEVKDILDEAERKVFAISEKRTTSNEGPQNICAILEK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHH
TIDRIELLSKTEGHNGITGVSTGFDALDKKTAGLQPSDLIIVAARPSMGKTTFAMNLCEN
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHH
AALKSDKPVLVFSLEMPAEQIMMRSLASLSRVDQTKIRTGQGLDQSDWSKIGSTMGLFAN
HCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
KPNLYIDDSSGLTPTELRSRARRVYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISR
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCEEHHHHHHH
SLKALAKELEVPVIALSQLNRTLENRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNE
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEEHHHCCC
NSEDKGVAEIIIGKQRNGPIGRVRLAFRGQFSRFDNLAEQRDIRDDY
CCCCCCHHHHEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AKKNAQIPSKPLEQGIVPPHSLEAEQSVLGGILIHPAHWDSISDMLIADDFYLAAHRVI
CCCCCCCCCCHHHHCCCCCCCCCHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHH
FQEMENLLRQGKPIDLINLYEFLREKNLSEDVGGFAYLAELSKNTPSVSNIVSYAAIVRD
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RAILRDLISVSNDVAQSCYTTKGMEVKDILDEAERKVFAISEKRTTSNEGPQNICAILEK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHH
TIDRIELLSKTEGHNGITGVSTGFDALDKKTAGLQPSDLIIVAARPSMGKTTFAMNLCEN
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHH
AALKSDKPVLVFSLEMPAEQIMMRSLASLSRVDQTKIRTGQGLDQSDWSKIGSTMGLFAN
HCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
KPNLYIDDSSGLTPTELRSRARRVYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISR
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCEEHHHHHHH
SLKALAKELEVPVIALSQLNRTLENRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNE
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEEHHHCCC
NSEDKGVAEIIIGKQRNGPIGRVRLAFRGQFSRFDNLAEQRDIRDDY
CCCCCCHHHHEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100