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The map label for this gene is fdxG_2 [H]

Identifier: 15602274

GI number: 15602274

Start: 478737

End: 479324

Strand: Reverse

Name: fdxG_2 [H]

Synonym: PM0409

Alternate gene names: 15602274

Gene position: 479324-478737 (Counterclockwise)

Preceding gene: 15602279

Following gene: 15602273

Centisome position: 21.23

GC content: 47.28

Gene sequence:

>588_bases
ATGCAGGTCAGTAGAAGAAAGTTCTTCAAGATCTGTGCAGGGGGAATGGCGGGGACATCTGCGGCAATGTTAGGCTTTGC
GCCAACAGAAGCGTTAGCAGCGCCTCGCAATTACAAATTATTACGCGCCAAAGAAACGCGTAATACCTGTACTTATTGCG
CTGTTGGTTGTGGTATGTTGTTGTACAGCTTGGGTGATGGCTCAAAAAACAGCAAAGGCAAACTCTTTCATATCGAGGGC
GACCCCGATCATCCAGTTAGTCGCGGTGCCTTATGTCCAAAAGGGGCAGGTGCTTTAGACTATGTCAATAGTGACCGTCG
TGTTAAATACCCTGAAGTGCGTGAAGCCGGTTCAAAAGAATGGAAACGGATTTCATGGCACGAAGCCATTGAGCGTATTG
CTCGTCACATCAAAGATGACCGTGATGCTAACTTCGTTGAAAAAAATGACGCAGGTGAGCCAGTCAATCGTTGGATGACA
GCAGGCTTCTTAGCGGGTTCGGCATGTAGTAACGAAACCGGCATCCTTACACAAAAATTTGTGCGTTCTCTCGGTATTAT
CTTTACCGACAACCAAGCGAGTATCTGA

Upstream 100 bases:

>100_bases
TAACAAGTGAAAAACAAGTCAAAATATTTAATTATCTATTTAACGTGTTTACGACTATCAAATGCTAACAAAATTTTTGT
TACGCTAAGGAGTATTATCT

Downstream 100 bases:

>100_bases
CACGGACCAACGGTAGCAAGTCTTGCTCCATCATTTGGTCGCGGTGCGATGACCAACCACTGGGTTGATATTAAAAACGC
AGATTTAGTGATTGTTATGG

Product: FdxG

Products: NA

Alternate protein names: Anaerobic formate dehydrogenase major subunit; Formate dehydrogenase-N subunit alpha; FDH-N subunit alpha [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MQVSRRKFFKICAGGMAGTSAAMLGFAPTEALAAPRNYKLLRAKETRNTCTYCAVGCGMLLYSLGDGSKNSKGKLFHIEG
DPDHPVSRGALCPKGAGALDYVNSDRRVKYPEVREAGSKEWKRISWHEAIERIARHIKDDRDANFVEKNDAGEPVNRWMT
AGFLAGSACSNETGILTQKFVRSLGIIFTDNQASI

Sequences:

>Translated_195_residues
MQVSRRKFFKICAGGMAGTSAAMLGFAPTEALAAPRNYKLLRAKETRNTCTYCAVGCGMLLYSLGDGSKNSKGKLFHIEG
DPDHPVSRGALCPKGAGALDYVNSDRRVKYPEVREAGSKEWKRISWHEAIERIARHIKDDRDANFVEKNDAGEPVNRWMT
AGFLAGSACSNETGILTQKFVRSLGIIFTDNQASI
>Mature_195_residues
MQVSRRKFFKICAGGMAGTSAAMLGFAPTEALAAPRNYKLLRAKETRNTCTYCAVGCGMLLYSLGDGSKNSKGKLFHIEG
DPDHPVSRGALCPKGAGALDYVNSDRRVKYPEVREAGSKEWKRISWHEAIERIARHIKDDRDANFVEKNDAGEPVNRWMT
AGFLAGSACSNETGILTQKFVRSLGIIFTDNQASI

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868719, Length=195, Percent_Identity=69.7435897435897, Blast_Score=301, Evalue=1e-83,
Organism=Escherichia coli, GI3868720, Length=195, Percent_Identity=66.1538461538461, Blast_Score=291, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006443
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.2.1.2 [H]

Molecular weight: Translated: 21378; Mature: 21378

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSRRKFFKICAGGMAGTSAAMLGFAPTEALAAPRNYKLLRAKETRNTCTYCAVGCGML
CCCHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
LYSLGDGSKNSKGKLFHIEGDPDHPVSRGALCPKGAGALDYVNSDRRVKYPEVREAGSKE
HHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHCCCHH
WKRISWHEAIERIARHIKDDRDANFVEKNDAGEPVNRWMTAGFLAGSACSNETGILTQKF
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
VRSLGIIFTDNQASI
HHHCCCEEECCCCCC
>Mature Secondary Structure
MQVSRRKFFKICAGGMAGTSAAMLGFAPTEALAAPRNYKLLRAKETRNTCTYCAVGCGML
CCCHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
LYSLGDGSKNSKGKLFHIEGDPDHPVSRGALCPKGAGALDYVNSDRRVKYPEVREAGSKE
HHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHCCCHH
WKRISWHEAIERIARHIKDDRDANFVEKNDAGEPVNRWMTAGFLAGSACSNETGILTQKF
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH
VRSLGIIFTDNQASI
HHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]