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The map label for this gene is fdxG_1 [H]

Identifier: 15602273

GI number: 15602273

Start: 476259

End: 478688

Strand: Reverse

Name: fdxG_1 [H]

Synonym: PM0408

Alternate gene names: 15602273

Gene position: 478688-476259 (Counterclockwise)

Preceding gene: 15602274

Following gene: 15602272

Centisome position: 21.2

GC content: 43.21

Gene sequence:

>2430_bases
ATGACCAACCACTGGGTTGATATTAAAAACGCAGATTTAGTGATTGTTATGGGCGGTAACGCAGCAGAAGCACACCCTGT
TGGATTCCGTTGGGCGATTGAAGCCAAAAAACAAAACGGTGCCAAACTGATGGTGGTCGATCCTCGTTTTAACCGTACTG
CAGCTGTCGCTGATATTTATATGCCTCTTCGTCCAGGTACGGATATTGCGTTCCTGTCTGGGGTGATTCGCTATTTGTTG
AAAAACGATAAAATCCAACACGAATATGTGAAACACTATACCAATGCCACCTTCCTCGTGAATGAAAACTTCAAATTTGA
AGACGGGCTATTCTCTGGTTATGACGAAGCCACACGCAAGTATGATCGCTCCACTTGGGCTTACCAATTTGATGAAAATG
GTCAGCCAAAACGTGATATGGATATGCAAGATCCACGCTGTGTGATCAATATGCTACGTGAACATGTGGAACGCTATACA
CCAGAAATGGTAGAACGTATTACTGGCACACCACAGAAAGACTTCCAAATCTTCTGTGAAGAAATTGCTAAAACCTCTGC
GCCAGATAAAGCAGCAACATTCCTTTATGCATTAGGTTGGACTCAACATACCGTTGGTTCACAAAATATTCGAACCATGG
CGATGATCCAATTATTACTCGGTAACATTGGTGTATCGGGTGGTGGTGTGAATGCATTACGTGGTCACTCAAACGTACAA
GGGATTACAGATTTAGGCTTATTCCCAAATCGTTTACCTGCATATATTCCATTACCAACAGAAGCTGATAAAAGCTTACA
GTCGTTCTTGGATCGTATCACACCAAAAACTATGATGAACGATCAGGTGAACTATTGGAAAAATACACCAAAATTCATGG
TCAGTATGCTGAAATCCTTCTATGGTGATAAAGGTACGCAAGATAACGAATTTGGTTATCACTATTTACCAAAATTACCG
AAAGGTGGTACCGACCAATTCCGTTACATCGAAGACATGTATAACGGTAAAGTGAATGGTTTCTTCTGCCAAGGGATGAA
CCCAGTTGCCTCTTACGCTAACTCACAAAAAATCATTAAAGCCTTAAGTAAACTAAAATATTTAGTGATTTTTGACCCAT
TAATCACCGATACCTCTGAATTCTGGAAAAACTACGGTGAATTTAATGATGTGAAAACCGAAGAGATTCAAACTGAAGTA
TTCCGTTTACCAACAACCTGCTTCGTCGAAGAAGATGGTTCCATTGCGAACTCCGGTCGTTGGCTACAATGGCACTGGAA
AGGCGCGGAGCCACCGGGTGAAGCCAAAACTGACGGTGAAATTCTTTCTGAATTGCGTGCTGAGCTTATCCACCTATATA
AAACAGAAGGCGGAAAAGCCCCACTTGAGCCACTAGAAGCAATGAGCTGGGATTATGCAAACCCACTTGAGCCAAAAGCG
GAAGAAGTCGCAAAAGAGAATAATGGTTACGCATTAGAAGACATCAAAGATGCAGACGGCAACATTATCTTGAAAAAAGG
TCAGTTACTGTCGAGCTTTGCACAAATGCGTGATGATGGCACGACATCAGGAGCTTGCTGGATTTACACGGGACAATGGA
CCGAAAAAGGTAACCAAATGGCAAACCGTGATAACGCTGATCCATCGAACTTAGGAAATACGCTAGGTTGGGCATTTGCT
TGGCCATTAAACCGCCGTATTCTTTATAACCGTGCAGGTGCAGACTTAGCCGGTAATCCATTTAACCCGAAACGTCAATT
GATCAAGTGGAACGGTAAAAACTGGAACTATGTGGATGTTGCCGACTACGGTACTGCCCCACCTAATAGCCCGGTTATGC
CATTTATCATGCAACCAGAAGGGGTAAGTGGCTTGTTCGTACGTGAACGTATGGCTGATGGTCCATTCCCAGAGCACTAT
GAACCCATGGAAACACCAATTGGTACTAACCCACTTCATCCAAATGTGGTATCAAGTCCAGTTGCACGGATTTTAGCCAG
TGATAAAGAAGATCTGGGAACAAGTGCGGATTTCCCATATGTGGGTACGACTTACCGTTTAACCGAACACTTCCATTATT
GGACGAAAAACGTATTACTGAACGTGATTGCACAACCAGAACAATTTGTTGAGATTGGTGAAGCCTTGGCGGCTGAAAAA
GGCATTAAACATGGCGATATCGTGAAAGTCAGCTCAAAACGCGGTTATATTAAAGCCGTTGCTGTGGTGACCAAACGTAT
CCGTGCTTTAGTGTCTGACGGCAAGCCAATTCATACCGTCGGAATTCCTATTCACTGGGGCTTTGCCGCAACGACTGGTG
CGAAAAAAGGTTTCTTCGCCAACAACTTAACTGTTCGTACTGGTGATGCGAATACACAAACACCAGAATCTAAATGTATG
TTAGTCAATATTGAGAAAGTGGGGGCGTAA

Upstream 100 bases:

>100_bases
ATTTGTGCGTTCTCTCGGTATTATCTTTACCGACAACCAAGCGAGTATCTGACACGGACCAACGGTAGCAAGTCTTGCTC
CATCATTTGGTCGCGGTGCG

Downstream 100 bases:

>100_bases
AATGGGTGTAGTACAATCTCAAAACATTATTAAAGCCTCTGCAACATCAAGCTTAACGCCACCACCACAAGCGCGTGAGC
ATCAAGTTGAAGTGGCAAAA

Product: FdxG

Products: NA

Alternate protein names: Anaerobic formate dehydrogenase major subunit; Formate dehydrogenase-N subunit alpha; FDH-N subunit alpha [H]

Number of amino acids: Translated: 809; Mature: 808

Protein sequence:

>809_residues
MTNHWVDIKNADLVIVMGGNAAEAHPVGFRWAIEAKKQNGAKLMVVDPRFNRTAAVADIYMPLRPGTDIAFLSGVIRYLL
KNDKIQHEYVKHYTNATFLVNENFKFEDGLFSGYDEATRKYDRSTWAYQFDENGQPKRDMDMQDPRCVINMLREHVERYT
PEMVERITGTPQKDFQIFCEEIAKTSAPDKAATFLYALGWTQHTVGSQNIRTMAMIQLLLGNIGVSGGGVNALRGHSNVQ
GITDLGLFPNRLPAYIPLPTEADKSLQSFLDRITPKTMMNDQVNYWKNTPKFMVSMLKSFYGDKGTQDNEFGYHYLPKLP
KGGTDQFRYIEDMYNGKVNGFFCQGMNPVASYANSQKIIKALSKLKYLVIFDPLITDTSEFWKNYGEFNDVKTEEIQTEV
FRLPTTCFVEEDGSIANSGRWLQWHWKGAEPPGEAKTDGEILSELRAELIHLYKTEGGKAPLEPLEAMSWDYANPLEPKA
EEVAKENNGYALEDIKDADGNIILKKGQLLSSFAQMRDDGTTSGACWIYTGQWTEKGNQMANRDNADPSNLGNTLGWAFA
WPLNRRILYNRAGADLAGNPFNPKRQLIKWNGKNWNYVDVADYGTAPPNSPVMPFIMQPEGVSGLFVRERMADGPFPEHY
EPMETPIGTNPLHPNVVSSPVARILASDKEDLGTSADFPYVGTTYRLTEHFHYWTKNVLLNVIAQPEQFVEIGEALAAEK
GIKHGDIVKVSSKRGYIKAVAVVTKRIRALVSDGKPIHTVGIPIHWGFAATTGAKKGFFANNLTVRTGDANTQTPESKCM
LVNIEKVGA

Sequences:

>Translated_809_residues
MTNHWVDIKNADLVIVMGGNAAEAHPVGFRWAIEAKKQNGAKLMVVDPRFNRTAAVADIYMPLRPGTDIAFLSGVIRYLL
KNDKIQHEYVKHYTNATFLVNENFKFEDGLFSGYDEATRKYDRSTWAYQFDENGQPKRDMDMQDPRCVINMLREHVERYT
PEMVERITGTPQKDFQIFCEEIAKTSAPDKAATFLYALGWTQHTVGSQNIRTMAMIQLLLGNIGVSGGGVNALRGHSNVQ
GITDLGLFPNRLPAYIPLPTEADKSLQSFLDRITPKTMMNDQVNYWKNTPKFMVSMLKSFYGDKGTQDNEFGYHYLPKLP
KGGTDQFRYIEDMYNGKVNGFFCQGMNPVASYANSQKIIKALSKLKYLVIFDPLITDTSEFWKNYGEFNDVKTEEIQTEV
FRLPTTCFVEEDGSIANSGRWLQWHWKGAEPPGEAKTDGEILSELRAELIHLYKTEGGKAPLEPLEAMSWDYANPLEPKA
EEVAKENNGYALEDIKDADGNIILKKGQLLSSFAQMRDDGTTSGACWIYTGQWTEKGNQMANRDNADPSNLGNTLGWAFA
WPLNRRILYNRAGADLAGNPFNPKRQLIKWNGKNWNYVDVADYGTAPPNSPVMPFIMQPEGVSGLFVRERMADGPFPEHY
EPMETPIGTNPLHPNVVSSPVARILASDKEDLGTSADFPYVGTTYRLTEHFHYWTKNVLLNVIAQPEQFVEIGEALAAEK
GIKHGDIVKVSSKRGYIKAVAVVTKRIRALVSDGKPIHTVGIPIHWGFAATTGAKKGFFANNLTVRTGDANTQTPESKCM
LVNIEKVGA
>Mature_808_residues
TNHWVDIKNADLVIVMGGNAAEAHPVGFRWAIEAKKQNGAKLMVVDPRFNRTAAVADIYMPLRPGTDIAFLSGVIRYLLK
NDKIQHEYVKHYTNATFLVNENFKFEDGLFSGYDEATRKYDRSTWAYQFDENGQPKRDMDMQDPRCVINMLREHVERYTP
EMVERITGTPQKDFQIFCEEIAKTSAPDKAATFLYALGWTQHTVGSQNIRTMAMIQLLLGNIGVSGGGVNALRGHSNVQG
ITDLGLFPNRLPAYIPLPTEADKSLQSFLDRITPKTMMNDQVNYWKNTPKFMVSMLKSFYGDKGTQDNEFGYHYLPKLPK
GGTDQFRYIEDMYNGKVNGFFCQGMNPVASYANSQKIIKALSKLKYLVIFDPLITDTSEFWKNYGEFNDVKTEEIQTEVF
RLPTTCFVEEDGSIANSGRWLQWHWKGAEPPGEAKTDGEILSELRAELIHLYKTEGGKAPLEPLEAMSWDYANPLEPKAE
EVAKENNGYALEDIKDADGNIILKKGQLLSSFAQMRDDGTTSGACWIYTGQWTEKGNQMANRDNADPSNLGNTLGWAFAW
PLNRRILYNRAGADLAGNPFNPKRQLIKWNGKNWNYVDVADYGTAPPNSPVMPFIMQPEGVSGLFVRERMADGPFPEHYE
PMETPIGTNPLHPNVVSSPVARILASDKEDLGTSADFPYVGTTYRLTEHFHYWTKNVLLNVIAQPEQFVEIGEALAAEKG
IKHGDIVKVSSKRGYIKAVAVVTKRIRALVSDGKPIHTVGIPIHWGFAATTGAKKGFFANNLTVRTGDANTQTPESKCML
VNIEKVGA

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868719, Length=807, Percent_Identity=66.1710037174721, Blast_Score=1148, Evalue=0.0,
Organism=Escherichia coli, GI3868720, Length=808, Percent_Identity=66.2128712871287, Blast_Score=1119, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=449, Percent_Identity=25.1670378619154, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1787870, Length=239, Percent_Identity=30.1255230125523, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI171474008, Length=233, Percent_Identity=26.6094420600858, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87081797, Length=233, Percent_Identity=26.1802575107296, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006443
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.2.1.2 [H]

Molecular weight: Translated: 90723; Mature: 90592

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHWVDIKNADLVIVMGGNAAEAHPVGFRWAIEAKKQNGAKLMVVDPRFNRTAAVADIY
CCCCEEEECCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCEEEEEEE
MPLRPGTDIAFLSGVIRYLLKNDKIQHEYVKHYTNATFLVNENFKFEDGLFSGYDEATRK
EECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHH
YDRSTWAYQFDENGQPKRDMDMQDPRCVINMLREHVERYTPEMVERITGTPQKDFQIFCE
HCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHH
EIAKTSAPDKAATFLYALGWTQHTVGSQNIRTMAMIQLLLGNIGVSGGGVNALRGHSNVQ
HHHHCCCCCHHHHHHHEECCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCC
GITDLGLFPNRLPAYIPLPTEADKSLQSFLDRITPKTMMNDQVNYWKNTPKFMVSMLKSF
CCHHCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHCCCHHHHCCCHHHHHHHHHHH
YGDKGTQDNEFGYHYLPKLPKGGTDQFRYIEDMYNGKVNGFFCQGMNPVASYANSQKIIK
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCHHHHHH
ALSKLKYLVIFDPLITDTSEFWKNYGEFNDVKTEEIQTEVFRLPTTCFVEEDGSIANSGR
HHHHCEEEEEECCHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCC
WLQWHWKGAEPPGEAKTDGEILSELRAELIHLYKTEGGKAPLEPLEAMSWDYANPLEPKA
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCHH
EEVAKENNGYALEDIKDADGNIILKKGQLLSSFAQMRDDGTTSGACWIYTGQWTEKGNQM
HHHHHCCCCEEECCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEECCHHHCCCHH
ANRDNADPSNLGNTLGWAFAWPLNRRILYNRAGADLAGNPFNPKRQLIKWNGKNWNYVDV
CCCCCCCHHHHCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCHHEEECCCCCCEEEE
ADYGTAPPNSPVMPFIMQPEGVSGLFVRERMADGPFPEHYEPMETPIGTNPLHPNVVSSP
CCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VARILASDKEDLGTSADFPYVGTTYRLTEHFHYWTKNVLLNVIAQPEQFVEIGEALAAEK
HHHHHHCCHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GIKHGDIVKVSSKRGYIKAVAVVTKRIRALVSDGKPIHTVGIPIHWGFAATTGAKKGFFA
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECEEEECCEECCCCCCCEEE
NNLTVRTGDANTQTPESKCMLVNIEKVGA
CCCEEEECCCCCCCCCCCEEEEEEHCCCC
>Mature Secondary Structure 
TNHWVDIKNADLVIVMGGNAAEAHPVGFRWAIEAKKQNGAKLMVVDPRFNRTAAVADIY
CCCEEEECCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCEEEEEEE
MPLRPGTDIAFLSGVIRYLLKNDKIQHEYVKHYTNATFLVNENFKFEDGLFSGYDEATRK
EECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHH
YDRSTWAYQFDENGQPKRDMDMQDPRCVINMLREHVERYTPEMVERITGTPQKDFQIFCE
HCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHH
EIAKTSAPDKAATFLYALGWTQHTVGSQNIRTMAMIQLLLGNIGVSGGGVNALRGHSNVQ
HHHHCCCCCHHHHHHHEECCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCC
GITDLGLFPNRLPAYIPLPTEADKSLQSFLDRITPKTMMNDQVNYWKNTPKFMVSMLKSF
CCHHCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHCCCHHHHCCCHHHHHHHHHHH
YGDKGTQDNEFGYHYLPKLPKGGTDQFRYIEDMYNGKVNGFFCQGMNPVASYANSQKIIK
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCHHHHHH
ALSKLKYLVIFDPLITDTSEFWKNYGEFNDVKTEEIQTEVFRLPTTCFVEEDGSIANSGR
HHHHCEEEEEECCHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCC
WLQWHWKGAEPPGEAKTDGEILSELRAELIHLYKTEGGKAPLEPLEAMSWDYANPLEPKA
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCHH
EEVAKENNGYALEDIKDADGNIILKKGQLLSSFAQMRDDGTTSGACWIYTGQWTEKGNQM
HHHHHCCCCEEECCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEECCHHHCCCHH
ANRDNADPSNLGNTLGWAFAWPLNRRILYNRAGADLAGNPFNPKRQLIKWNGKNWNYVDV
CCCCCCCHHHHCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCHHEEECCCCCCEEEE
ADYGTAPPNSPVMPFIMQPEGVSGLFVRERMADGPFPEHYEPMETPIGTNPLHPNVVSSP
CCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VARILASDKEDLGTSADFPYVGTTYRLTEHFHYWTKNVLLNVIAQPEQFVEIGEALAAEK
HHHHHHCCHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GIKHGDIVKVSSKRGYIKAVAVVTKRIRALVSDGKPIHTVGIPIHWGFAATTGAKKGFFA
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECEEEECCEECCCCCCCEEE
NNLTVRTGDANTQTPESKCMLVNIEKVGA
CCCEEEECCCCCCCCCCCEEEEEEHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]